Rroxscaffold_2G00143630

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0031954
Physical Location & Seq
Reverse (-)
81624487 .. 81625819
1333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00143630.1

Sequence Viewer

Length: 993 bp
ATGAAGCAGATCCACCAGGAGAATTCTGGCGGAGAGATAGCTATGAAGCCTATGATCAGTACTGCAGAAGAACTAGCCACAGGTACCAGAATGGGCGGGGAATCCACTGTACAAGAAATAGAGTCTCCGGCGGAACCTGCTCCCATAGTTGCGAAAGCATTAGATCAGTCTCCGAAGGAACCATCTACCCCCACGGTTGCGAAAGGACTAGATCAGTCTCTGAAGGAACCACCTGCCCCCGCAGTTGTGAAATCCACATACCAGTGTCTCACGGAACCAACAGCTGCCCCCGCAGTTGCAAAAGCAATATATCAGTCTCCGACGGAACCAGCTGGCCCCGCAGTTGTGAAAGAAAAAGAGTCTCGGAAGGAACCAGCCGCCCCGGCAGTTGCGAAAGCCGTAGACCAGTCTCCCCAGGAACCAGCTGCCCCCGCAGTTGCAGATGCAATAGAGGATACAACAGAGAGAACCAGCTCCCCACGGAGTTGCAGATGCAGTTGGAGATGCATGTGTCTTCCATATGGAACCAGTACTACTACTAGTAGTGCACATTTCCGAACAACAATACTGGCGCGGTACCTTGCAAAGTGGAATATTTTGTTTGCAATATCATGTGTGCTGGCTGTCTGCACGGATCCTTTGTATCTTTATGTTCCTCTGGTTAACGACGACAGGAAGTGTGTCAGGTTCGACAAGAAGATGCAGATGATAGTTCTCGTTTTCCGAGCATTAACTGATGCATTTTACATTATAGACATCTTGTTTCATAGCTTTACTAGATTTGAATCTCCCGGATATGATCCCTTAGCAGGGGAAACAACACAAACTGACGAAGAAGTAGCTTTGATTAACAAGGAAGGGGGGAATTCCATCAACACAAATCGGAAAATATTTCTCATAATCCTAGTTGACGTGCTAGCCATCTTGCCGGTCACATATGTACGAGAAGATGGAGAGAGTGGTTTTCACTTCTCCCTAACTATCAATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans Pkinase_Tyr cNMP_binding

Protein Analysis

330

Amino Acids

35.93

Weight (kDa)

5.22

Isoelectric Point (pI)

63.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031954)

Species Orthologous Gene IDs
rosa_roxburghii Rroxscaffold_2G00143630
rosa_wichuraiana Rw2G009540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 241
Acc36I ACCTGC 2 cut(s) 145, 241
Acc65I GGTACC 2 cut(s) 83, 576
AccB1I GGYRCC 2 cut(s) 83, 576
AccI GTMKAC 1 cut(s) 402
AccII CGCG 1 cut(s) 574
AciI CCGC 9 cut(s) 30, 96, 131, 240, 291, 339, 378, 432, 574
AclWI GGATC 4 cut(s) 4, 629, 642, 794
AcsI RAATTY 2 cut(s) 22, 865
AcuI CTGAAG 1 cut(s) 242
AfaI GTAC 6 cut(s) 61, 85, 111, 532, 578, 942
AfiI CCNNNNNNNGG 2 cut(s) 809, 810
AgsI TTSAA 1 cut(s) 785
AhlI ACTAGT 1 cut(s) 539
AjiI CACGTC 1 cut(s) 913
AjnI CCWGG 2 cut(s) 15, 414
AluBI AGCT 7 cut(s) 41, 284, 332, 425, 474, 771, 842
AluI AGCT 7 cut(s) 41, 284, 332, 425, 474, 771, 842
Alw21I GWGCWC 1 cut(s) 550
Alw26I GTCTC 7 cut(s) 129, 174, 222, 272, 321, 366, 414
Alw44I GTGCAC 1 cut(s) 546
AlwI GGATC 4 cut(s) 4, 629, 642, 794
AlwNI CAGNNNCTG 1 cut(s) 220
AoxI GGCC 1 cut(s) 334
ApaLI GTGCAC 1 cut(s) 546
ApeKI GCWGC 2 cut(s) 284, 425
ApoI RAATTY 2 cut(s) 22, 865
Asp718I GGTACC 2 cut(s) 83, 576
AspLEI GCGC 1 cut(s) 574
AspS9I GGNCC 1 cut(s) 335
AsuC2I CCSGG 2 cut(s) 383, 792
AsuNHI GCTAGC 1 cut(s) 916
BaeGI GKGCMC 1 cut(s) 550
BamHI GGATCC 1 cut(s) 634
BanI GGYRCC 2 cut(s) 83, 576
BbsI GAAGAC 1 cut(s) 506
Bbv12I GWGCWC 1 cut(s) 550
BbvI GCAGC 2 cut(s) 271, 412
BccI CCATC 4 cut(s) 190, 878, 929, 944
BceAI ACGGC 1 cut(s) 383
BciT130I CCWGG 2 cut(s) 17, 416
BciVI GTATCC 1 cut(s) 448
BclI TGATCA 1 cut(s) 54
BcnI CCSGG 2 cut(s) 383, 792
BcoDI GTCTC 7 cut(s) 129, 174, 222, 272, 321, 366, 414
BcuI ACTAGT 1 cut(s) 539
BfaI CTAG 6 cut(s) 74, 209, 540, 777, 905, 917
BfmI CTRYAG 1 cut(s) 63
BfuAI ACCTGC 2 cut(s) 145, 241
BfuI GTATCC 1 cut(s) 448
BglI GCCNNNNNGGC 1 cut(s) 383
BisI GCNGC 3 cut(s) 285, 378, 426
BlsI GCNGC 3 cut(s) 286, 379, 427
BmcAI AGTACT 2 cut(s) 61, 532
Bme1390I CCNGG 4 cut(s) 17, 383, 416, 792
BmgBI CACGTC 1 cut(s) 913
BmgT120I GGNCC 1 cut(s) 335
BmrFI CCNGG 4 cut(s) 17, 383, 416, 792
BmsI GCATC 5 cut(s) 433, 482, 494, 690, 727
BmtI GCTAGC 1 cut(s) 920
BpiI GAAGAC 1 cut(s) 506
Bpu10I CCTNAGC 1 cut(s) 805
BpuMI CCSGG 2 cut(s) 383, 792
BsaBI GATNNNNATC 1 cut(s) 784
BsaJI CCNNGG 4 cut(s) 192, 381, 414, 479
Bsc4I CCNNNNNNNGG 2 cut(s) 809, 810
Bse118I RCCGGY 1 cut(s) 928
Bse1I ACTGG 4 cut(s) 262, 406, 528, 573
Bse8I GATNNNNATC 1 cut(s) 784
BseBI CCWGG 2 cut(s) 17, 416
BseDI CCNNGG 4 cut(s) 192, 381, 414, 479
BseJI GATNNNNATC 1 cut(s) 784
BseLI CCNNNNNNNGG 2 cut(s) 809, 810
BseNI ACTGG 4 cut(s) 262, 406, 528, 573
BseSI GKGCMC 1 cut(s) 550
BseXI GCAGC 2 cut(s) 271, 412
BsgI GTGCAG 1 cut(s) 613
Bsh1236I CGCG 1 cut(s) 574
BshFI GGCC 1 cut(s) 336
BshNI GGYRCC 2 cut(s) 83, 576
BsiHKAI GWGCWC 1 cut(s) 550
BsiSI CCGG 4 cut(s) 128, 383, 792, 929
BslI CCNNNNNNNGG 2 cut(s) 809, 810
BsmAI GTCTC 7 cut(s) 129, 174, 222, 272, 321, 366, 414
BsnI GGCC 1 cut(s) 336
Bsp1286I GDGCHC 1 cut(s) 550
Bsp1407I TGTACA 1 cut(s) 109
Bsp143I GATC 6 cut(s) 9, 54, 163, 211, 634, 799
BspACI CCGC 9 cut(s) 30, 96, 131, 240, 291, 339, 378, 432, 574
BspANI GGCC 1 cut(s) 336
BspFNI CGCG 1 cut(s) 574
BspMAI CTGCAG 1 cut(s) 67
BspMI ACCTGC 2 cut(s) 145, 241
BspOI GCTAGC 1 cut(s) 920
BspPI GGATC 4 cut(s) 4, 629, 642, 794
BspT107I GGYRCC 2 cut(s) 83, 576
BsrFI RCCGGY 1 cut(s) 928
BsrGI TGTACA 1 cut(s) 109
BsrI ACTGG 4 cut(s) 262, 406, 528, 573
BssAI RCCGGY 1 cut(s) 928
BssECI CCNNGG 4 cut(s) 192, 381, 414, 479
BssMI GATC 6 cut(s) 9, 54, 163, 211, 634, 799
Bst2UI CCWGG 2 cut(s) 17, 416
Bst4CI ACNGT 2 cut(s) 109, 196
BstAUI TGTACA 1 cut(s) 109
BstC8I GCNNGC 3 cut(s) 334, 621, 918
BstDEI CTNAG 1 cut(s) 805
BstDSI CCRYGG 2 cut(s) 192, 479
BstFNI CGCG 1 cut(s) 574
BstHHI GCGC 1 cut(s) 574
BstKTI GATC 6 cut(s) 12, 57, 166, 214, 637, 802
BstMAI GTCTC 7 cut(s) 129, 174, 222, 272, 321, 366, 414
BstMBI GATC 6 cut(s) 9, 54, 163, 211, 634, 799
BstMWI GCNNNNNNNGC 5 cut(s) 137, 290, 338, 383, 431
BstNI CCWGG 2 cut(s) 17, 416
BstNSI RCATGY 1 cut(s) 511
BstSCI CCNGG 4 cut(s) 15, 381, 414, 790
BstSFI CTRYAG 1 cut(s) 63
BstSLI GKGCMC 1 cut(s) 550
BstUI CGCG 1 cut(s) 574
BstV1I GCAGC 2 cut(s) 271, 412
BstV2I GAAGAC 1 cut(s) 506
BstX2I RGATCY 2 cut(s) 9, 634
BstYI RGATCY 2 cut(s) 9, 634
BsuI GTATCC 1 cut(s) 448
BsuRI GGCC 1 cut(s) 336
BtgI CCRYGG 2 cut(s) 192, 479
BtrI CACGTC 1 cut(s) 913
BtsIMutI CAGTG 2 cut(s) 105, 269
BveI ACCTGC 2 cut(s) 145, 241
Cac8I GCNNGC 3 cut(s) 334, 621, 918
CaiI CAGNNNCTG 1 cut(s) 220
CfoI GCGC 1 cut(s) 574
Cfr10I RCCGGY 1 cut(s) 928
Cfr13I GGNCC 1 cut(s) 335
Csp6I GTAC 6 cut(s) 60, 84, 110, 531, 577, 941
CviAII CATG 2 cut(s) 508, 612
CviQI GTAC 6 cut(s) 60, 84, 110, 531, 577, 941
DdeI CTNAG 1 cut(s) 805
DpnI GATC 6 cut(s) 11, 56, 165, 213, 636, 801
DpnII GATC 6 cut(s) 9, 54, 163, 211, 634, 799
EciI GGCGGA 2 cut(s) 45, 146
Eco57I CTGAAG 1 cut(s) 242
EcoRI GAATTC 2 cut(s) 22, 865
EcoRII CCWGG 2 cut(s) 15, 414
EcoT22I ATGCAT 2 cut(s) 509, 742
FaeI CATG 2 cut(s) 511, 615
FatI CATG 2 cut(s) 507, 611
FauI CCCGC 5 cut(s) 89, 247, 298, 346, 439
FauNDI CATATG 2 cut(s) 520, 937
FbaI TGATCA 1 cut(s) 54
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 3 cut(s) 285, 378, 426
Fsp4HI GCNGC 3 cut(s) 285, 378, 426
FspBI CTAG 6 cut(s) 74, 209, 540, 777, 905, 917
GlaI GCGC 1 cut(s) 573
GluI GCNGC 3 cut(s) 285, 378, 426
HaeIII GGCC 1 cut(s) 336
HapII CCGG 4 cut(s) 128, 383, 792, 929
HhaI GCGC 1 cut(s) 574
Hin1II CATG 2 cut(s) 511, 615
Hin6I GCGC 1 cut(s) 572
HinP1I GCGC 1 cut(s) 572
HincII GTYRAC 2 cut(s) 664, 910
HindII GTYRAC 2 cut(s) 664, 910
HinfI GANTC 4 cut(s) 101, 122, 359, 785
HpaI GTTAAC 1 cut(s) 664
HpaII CCGG 4 cut(s) 128, 383, 792, 929
Hpy166II GTNNAC 4 cut(s) 403, 548, 664, 910
Hpy188I TCNGA 7 cut(s) 174, 222, 321, 366, 557, 725, 885
Hpy8I GTNNAC 4 cut(s) 403, 548, 664, 910
Hpy99I CGWCG 2 cut(s) 325, 671
HpyAV CCTTC 4 cut(s) 169, 217, 361, 851
HpyCH4III ACNGT 2 cut(s) 109, 196
HpyCH4IV ACGT 1 cut(s) 912
HpyF10VI GCNNNNNNNGC 5 cut(s) 137, 290, 338, 383, 431
HpyF3I CTNAG 1 cut(s) 805
HpySE526I ACGT 1 cut(s) 912
Hsp92II CATG 2 cut(s) 511, 615
HspAI GCGC 1 cut(s) 572
KpnI GGTACC 2 cut(s) 87, 580
Ksp22I TGATCA 1 cut(s) 54
KspAI GTTAAC 1 cut(s) 664
Kzo9I GATC 6 cut(s) 9, 54, 163, 211, 634, 799
LmnI GCTCC 2 cut(s) 145, 479
Lsp1109I GCAGC 2 cut(s) 271, 412
LweI GCATC 5 cut(s) 433, 482, 494, 690, 727
MaeI CTAG 6 cut(s) 74, 209, 540, 777, 905, 917
MaeII ACGT 1 cut(s) 912
MaeIII GTNAC 1 cut(s) 931
MalI GATC 6 cut(s) 11, 56, 165, 213, 636, 801
MboI GATC 6 cut(s) 9, 54, 163, 211, 634, 799
MboII GAAGA 5 cut(s) 80, 506, 709, 845, 959
MflI RGATCY 2 cut(s) 9, 634
MhlI GDGCHC 1 cut(s) 550
MluCI AATT 3 cut(s) 22, 865, 985
MlyI GAGTC 2 cut(s) 131, 368
MmeI TCCRAC 2 cut(s) 344, 479
MnlI CCTC 2 cut(s) 445, 666
Mph1103I ATGCAT 2 cut(s) 509, 742
MseI TTAA 3 cut(s) 663, 731, 849
MslI CAYNNNNRTG 1 cut(s) 262
MspA1I CMGCKG 3 cut(s) 284, 332, 425
MspI CCGG 4 cut(s) 128, 383, 792, 929
MspR9I CCNGG 4 cut(s) 17, 383, 416, 792
MvaI CCWGG 2 cut(s) 17, 416
MvnI CGCG 1 cut(s) 574
MwoI GCNNNNNNNGC 5 cut(s) 137, 290, 338, 383, 431
NciI CCSGG 2 cut(s) 383, 792
NdeI CATATG 2 cut(s) 520, 937
NdeII GATC 6 cut(s) 9, 54, 163, 211, 634, 799
NheI GCTAGC 1 cut(s) 916
NlaIII CATG 2 cut(s) 511, 615
NmuCI GTSAC 1 cut(s) 931
NsiI ATGCAT 2 cut(s) 509, 742
NspI RCATGY 1 cut(s) 511
PaqCI CACCTGC 1 cut(s) 241
PfeI GAWTC 2 cut(s) 101, 785
PfoI TCCNGGA 1 cut(s) 790
PkrI GCNGC 3 cut(s) 286, 379, 427
PleI GAGTC 2 cut(s) 130, 367
PpsI GAGTC 2 cut(s) 130, 367
Psp6I CCWGG 2 cut(s) 15, 414
PspGI CCWGG 2 cut(s) 15, 414
PspPI GGNCC 1 cut(s) 335
PsrI GAACNNNNNNTAC 2 cut(s) 517, 549
PstI CTGCAG 1 cut(s) 67
PstNI CAGNNNCTG 1 cut(s) 220
PsuI RGATCY 2 cut(s) 9, 634
PvuII CAGCTG 3 cut(s) 284, 332, 425
RsaI GTAC 6 cut(s) 61, 85, 111, 532, 578, 942
RsaNI GTAC 6 cut(s) 60, 84, 110, 531, 577, 941
RseI CAYNNNNRTG 1 cut(s) 262
SaqAI TTAA 3 cut(s) 663, 731, 849
SatI GCNGC 3 cut(s) 285, 378, 426
Sau3AI GATC 6 cut(s) 9, 54, 163, 211, 634, 799
Sau96I GGNCC 1 cut(s) 335
ScaI AGTACT 2 cut(s) 61, 532
SchI GAGTC 2 cut(s) 131, 368
ScrFI CCNGG 4 cut(s) 17, 383, 416, 792
SduI GDGCHC 1 cut(s) 550
SfaNI GCATC 5 cut(s) 433, 482, 494, 690, 727
SfcI CTRYAG 1 cut(s) 63
SmiMI CAYNNNNRTG 1 cut(s) 262
SpeI ACTAGT 1 cut(s) 539
Sse9I AATT 3 cut(s) 22, 865, 985
SsiI CCGC 9 cut(s) 30, 96, 131, 240, 291, 339, 378, 432, 574
SspI AATATT 2 cut(s) 595, 891
SspMI CTAG 6 cut(s) 74, 209, 540, 777, 905, 917
StyD4I CCNGG 4 cut(s) 15, 381, 414, 790
TaaI ACNGT 2 cut(s) 109, 196
TaiI ACGT 1 cut(s) 915
TaqI TCGA 1 cut(s) 690
TasI AATT 3 cut(s) 22, 865, 985
TatI WGTACW 3 cut(s) 59, 109, 530
TauI GCSGC 1 cut(s) 380
TfiI GAWTC 2 cut(s) 101, 785
Tru1I TTAA 3 cut(s) 663, 731, 849
Tru9I TTAA 3 cut(s) 663, 731, 849
TscAI CASTG 2 cut(s) 112, 269
TseFI GTSAC 1 cut(s) 931
TseI GCWGC 2 cut(s) 284, 425
Tsp45I GTSAC 1 cut(s) 931
TspDTI ATGAA 3 cut(s) 17, 59, 755
TspGWI ACGGA 4 cut(s) 287, 338, 496, 647
TspRI CASTG 2 cut(s) 112, 269
VneI GTGCAC 1 cut(s) 546
XapI RAATTY 2 cut(s) 22, 865
XceI RCATGY 1 cut(s) 511
XcmI CCANNNNNNNNNTGG 1 cut(s) 23
XmiI GTMKAC 1 cut(s) 402
XspI CTAG 6 cut(s) 74, 209, 540, 777, 905, 917
ZrmI AGTACT 2 cut(s) 61, 532
Zsp2I ATGCAT 2 cut(s) 509, 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.