Rw2G009540

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0031954
Physical Location & Seq
Forward (+)
9700867 .. 9701998
1132 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G009540.1

Sequence Viewer

Length: 945 bp
ATGAAGCAAAAGATGTTAGTTTCTTTCATTCTACCTTCCAAGACCAGTAGCTGCAGTACTGCAGAAGAACTAGCCACAGGTACCAGAATGGGCGGGGAATCCACTGTAAAAGAAATAGAGTCTCCGGCTGAACCTGCTCCCGCAGTTGCGAAAGCATTAGATCAGTCTCCGAAGGAACCACCTACCCCCACGGTTGCAAAAGGACTAGATCAGTCTCTGAAGGAACCACCTGCCCCCGCAGTTGTGAAAGCAATATATCAATCTCCGACGGAACCAGCTTGCCCCGCAGTTGCGAAAGAAAAAGAGTCTCGGAAGGAACCAGCCGCCCTAGCAGTTGCGAAAGCCGTAGACCAGTCTCCCCAGGAACCAGCTGCCCCCGCAGTTGCAGATGCAATAGAGGATACAACAGAGAGAACCAGCTCCCCACGGAGTTGCAGTTACAGTTGGAGATGCATGTGTCTTCCATATGGAACCAGTACTACTACTAGTGCACATTTCCGAACAACAATACCGGCGCGGTACCTTGCAAAGTGGAATATTTTGTTTGCAATATCATGTGTGCTGGCTGTCTGCACGGATCCTTTGTATCTTTTTGTTCCTCTGGTTAACGACGACAGGAAGTGTGTCAGGTTCAACAAGAAGATACAGATGATAGTTCTCGTTTTCCGAGCATTAACTGATGCATTTTACACTATAGACATCTTGTTTCATAGCTTTACTAGATTTGAATCTCCCGGATATGATCCCTTAGGAGGGGAAACAACACAAACTGACGAAGAAGAAGCTTTGATTAACAAGGAAGGGGGGAATTCCATCAACACAAATCGGAAAATATTTTTCATAATCCTAGTTGACGTGCTAGCCATCTTGCCGGTCACATATGTACGAGAAGCTATCCCTAATGGAGAGAGTGGTTTTCACTTCTCCCTAACTATCAATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans Pkinase_Tyr cNMP_binding

Protein Analysis

314

Amino Acids

34.26

Weight (kDa)

5.59

Isoelectric Point (pI)

66.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031954)

Species Orthologous Gene IDs
rosa_roxburghii Rroxscaffold_2G00143630
rosa_wichuraiana Rw2G009540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 238
Acc36I ACCTGC 2 cut(s) 142, 238
Acc65I GGTACC 2 cut(s) 80, 519
AccB1I GGYRCC 2 cut(s) 80, 519
AccI GTMKAC 1 cut(s) 348
AccII CGCG 1 cut(s) 517
AciI CCGC 7 cut(s) 93, 141, 237, 285, 324, 378, 517
AclWI GGATC 3 cut(s) 572, 585, 737
AcsI RAATTY 1 cut(s) 808
AcuI CTGAAG 1 cut(s) 239
AfaI GTAC 5 cut(s) 58, 82, 478, 521, 885
AfiI CCNNNNNNNGG 2 cut(s) 752, 753
AgsI TTSAA 2 cut(s) 634, 728
AhlI ACTAGT 1 cut(s) 485
AjiI CACGTC 1 cut(s) 856
AjnI CCWGG 1 cut(s) 360
AluBI AGCT 7 cut(s) 51, 278, 371, 420, 714, 785, 893
AluI AGCT 7 cut(s) 51, 278, 371, 420, 714, 785, 893
Alw21I GWGCWC 1 cut(s) 493
Alw26I GTCTC 5 cut(s) 126, 171, 219, 312, 360
Alw44I GTGCAC 1 cut(s) 489
AlwI GGATC 3 cut(s) 572, 585, 737
AlwNI CAGNNNCTG 2 cut(s) 51, 217
ApaLI GTGCAC 1 cut(s) 489
ApeKI GCWGC 2 cut(s) 51, 371
ApoI RAATTY 1 cut(s) 808
Asp718I GGTACC 2 cut(s) 80, 519
AspLEI GCGC 1 cut(s) 517
AsuC2I CCSGG 1 cut(s) 735
AsuNHI GCTAGC 1 cut(s) 859
AxyI CCTNAGG 1 cut(s) 748
BaeGI GKGCMC 1 cut(s) 493
BamHI GGATCC 1 cut(s) 577
BanI GGYRCC 2 cut(s) 80, 519
BbsI GAAGAC 1 cut(s) 452
Bbv12I GWGCWC 1 cut(s) 493
BbvI GCAGC 2 cut(s) 38, 358
BccI CCATC 2 cut(s) 821, 872
BceAI ACGGC 1 cut(s) 329
BciT130I CCWGG 1 cut(s) 362
BciVI GTATCC 1 cut(s) 394
BcnI CCSGG 1 cut(s) 735
BcoDI GTCTC 5 cut(s) 126, 171, 219, 312, 360
BcuI ACTAGT 1 cut(s) 485
BfaI CTAG 7 cut(s) 71, 206, 329, 486, 720, 848, 860
BfmI CTRYAG 3 cut(s) 52, 60, 693
BfuAI ACCTGC 2 cut(s) 142, 238
BfuI GTATCC 1 cut(s) 394
BisI GCNGC 3 cut(s) 52, 324, 372
BlsI GCNGC 3 cut(s) 53, 325, 373
BmcAI AGTACT 2 cut(s) 58, 478
Bme1390I CCNGG 2 cut(s) 362, 735
BmgBI CACGTC 1 cut(s) 856
BmiI GGNNCC 9 cut(s) 82, 177, 225, 273, 318, 366, 472, 521, 579
BmrFI CCNGG 2 cut(s) 362, 735
BmsI GCATC 3 cut(s) 379, 440, 670
BmtI GCTAGC 1 cut(s) 863
BpiI GAAGAC 1 cut(s) 452
BpuMI CCSGG 1 cut(s) 735
BsaBI GATNNNNATC 1 cut(s) 727
BsaJI CCNNGG 3 cut(s) 189, 360, 425
BsaXI ACNNNNNCTCC 2 cut(s) 421, 451
Bsc4I CCNNNNNNNGG 2 cut(s) 752, 753
Bse118I RCCGGY 2 cut(s) 511, 871
Bse1I ACTGG 3 cut(s) 45, 352, 474
Bse21I CCTNAGG 1 cut(s) 748
Bse8I GATNNNNATC 1 cut(s) 727
BseBI CCWGG 1 cut(s) 362
BseDI CCNNGG 3 cut(s) 189, 360, 425
BseJI GATNNNNATC 1 cut(s) 727
BseLI CCNNNNNNNGG 2 cut(s) 752, 753
BseNI ACTGG 3 cut(s) 45, 352, 474
BseSI GKGCMC 1 cut(s) 493
BseXI GCAGC 2 cut(s) 38, 358
BsgI GTGCAG 1 cut(s) 556
Bsh1236I CGCG 1 cut(s) 517
BshNI GGYRCC 2 cut(s) 80, 519
BsiHKAI GWGCWC 1 cut(s) 493
BsiSI CCGG 4 cut(s) 125, 512, 735, 872
BslI CCNNNNNNNGG 2 cut(s) 752, 753
BsmAI GTCTC 5 cut(s) 126, 171, 219, 312, 360
Bsp1286I GDGCHC 1 cut(s) 493
Bsp143I GATC 4 cut(s) 160, 208, 577, 742
BspACI CCGC 7 cut(s) 93, 141, 237, 285, 324, 378, 517
BspFNI CGCG 1 cut(s) 517
BspLI GGNNCC 9 cut(s) 82, 177, 225, 273, 318, 366, 472, 521, 579
BspMAI CTGCAG 2 cut(s) 56, 64
BspMI ACCTGC 2 cut(s) 142, 238
BspOI GCTAGC 1 cut(s) 863
BspPI GGATC 3 cut(s) 572, 585, 737
BspT107I GGYRCC 2 cut(s) 80, 519
BsrFI RCCGGY 2 cut(s) 511, 871
BsrI ACTGG 3 cut(s) 45, 352, 474
BssAI RCCGGY 2 cut(s) 511, 871
BssECI CCNNGG 3 cut(s) 189, 360, 425
BssMI GATC 4 cut(s) 160, 208, 577, 742
Bst2UI CCWGG 1 cut(s) 362
Bst4CI ACNGT 3 cut(s) 106, 193, 443
BstC8I GCNNGC 3 cut(s) 280, 564, 861
BstDEI CTNAG 1 cut(s) 748
BstDSI CCRYGG 2 cut(s) 189, 425
BstFNI CGCG 1 cut(s) 517
BstHHI GCGC 1 cut(s) 517
BstKTI GATC 4 cut(s) 163, 211, 580, 745
BstMAI GTCTC 5 cut(s) 126, 171, 219, 312, 360
BstMBI GATC 4 cut(s) 160, 208, 577, 742
BstMWI GCNNNNNNNGC 4 cut(s) 134, 284, 329, 377
BstNI CCWGG 1 cut(s) 362
BstNSI RCATGY 1 cut(s) 457
BstSCI CCNGG 2 cut(s) 360, 733
BstSFI CTRYAG 3 cut(s) 52, 60, 693
BstSLI GKGCMC 1 cut(s) 493
BstUI CGCG 1 cut(s) 517
BstV1I GCAGC 2 cut(s) 38, 358
BstV2I GAAGAC 1 cut(s) 452
BstX2I RGATCY 1 cut(s) 577
BstYI RGATCY 1 cut(s) 577
Bsu36I CCTNAGG 1 cut(s) 748
BsuI GTATCC 1 cut(s) 394
BtgI CCRYGG 2 cut(s) 189, 425
BtrI CACGTC 1 cut(s) 856
BtsIMutI CAGTG 1 cut(s) 102
BveI ACCTGC 2 cut(s) 142, 238
Cac8I GCNNGC 3 cut(s) 280, 564, 861
CaiI CAGNNNCTG 2 cut(s) 51, 217
CfoI GCGC 1 cut(s) 517
Cfr10I RCCGGY 2 cut(s) 511, 871
Csp6I GTAC 5 cut(s) 57, 81, 477, 520, 884
CviAII CATG 2 cut(s) 454, 555
CviQI GTAC 5 cut(s) 57, 81, 477, 520, 884
DdeI CTNAG 1 cut(s) 748
DpnI GATC 4 cut(s) 162, 210, 579, 744
DpnII GATC 4 cut(s) 160, 208, 577, 742
Eco57I CTGAAG 1 cut(s) 239
Eco81I CCTNAGG 1 cut(s) 748
EcoRI GAATTC 1 cut(s) 808
EcoRII CCWGG 1 cut(s) 360
EcoT22I ATGCAT 2 cut(s) 455, 685
FaeI CATG 2 cut(s) 457, 558
FatI CATG 2 cut(s) 453, 554
FauI CCCGC 5 cut(s) 86, 148, 244, 292, 385
FauNDI CATATG 2 cut(s) 466, 880
FblI GTMKAC 1 cut(s) 348
Fnu4HI GCNGC 3 cut(s) 52, 324, 372
Fsp4HI GCNGC 3 cut(s) 52, 324, 372
FspBI CTAG 7 cut(s) 71, 206, 329, 486, 720, 848, 860
GlaI GCGC 1 cut(s) 516
GluI GCNGC 3 cut(s) 52, 324, 372
HapII CCGG 4 cut(s) 125, 512, 735, 872
HhaI GCGC 1 cut(s) 517
Hin1II CATG 2 cut(s) 457, 558
Hin6I GCGC 1 cut(s) 515
HinP1I GCGC 1 cut(s) 515
HincII GTYRAC 2 cut(s) 607, 853
HindII GTYRAC 2 cut(s) 607, 853
HindIII AAGCTT 1 cut(s) 783
HinfI GANTC 4 cut(s) 98, 119, 305, 728
HpaI GTTAAC 1 cut(s) 607
HpaII CCGG 4 cut(s) 125, 512, 735, 872
Hpy166II GTNNAC 4 cut(s) 349, 491, 607, 853
Hpy188I TCNGA 7 cut(s) 171, 219, 267, 312, 500, 668, 828
Hpy8I GTNNAC 4 cut(s) 349, 491, 607, 853
Hpy99I CGWCG 2 cut(s) 271, 614
HpyAV CCTTC 5 cut(s) 45, 166, 214, 307, 794
HpyCH4III ACNGT 3 cut(s) 106, 193, 443
HpyCH4IV ACGT 1 cut(s) 855
HpyF10VI GCNNNNNNNGC 4 cut(s) 134, 284, 329, 377
HpyF3I CTNAG 1 cut(s) 748
HpySE526I ACGT 1 cut(s) 855
Hsp92II CATG 2 cut(s) 457, 558
HspAI GCGC 1 cut(s) 515
KpnI GGTACC 2 cut(s) 84, 523
KspAI GTTAAC 1 cut(s) 607
Kzo9I GATC 4 cut(s) 160, 208, 577, 742
LmnI GCTCC 2 cut(s) 142, 425
Lsp1109I GCAGC 2 cut(s) 38, 358
LweI GCATC 3 cut(s) 379, 440, 670
MaeI CTAG 7 cut(s) 71, 206, 329, 486, 720, 848, 860
MaeII ACGT 1 cut(s) 855
MaeIII GTNAC 2 cut(s) 437, 874
MalI GATC 4 cut(s) 162, 210, 579, 744
MboI GATC 4 cut(s) 160, 208, 577, 742
MboII GAAGA 5 cut(s) 77, 452, 652, 788, 791
MflI RGATCY 1 cut(s) 577
MhlI GDGCHC 1 cut(s) 493
MluCI AATT 2 cut(s) 808, 937
MlyI GAGTC 2 cut(s) 128, 314
MmeI TCCRAC 2 cut(s) 290, 425
MnlI CCTC 3 cut(s) 391, 609, 746
Mph1103I ATGCAT 2 cut(s) 455, 685
MseI TTAA 3 cut(s) 606, 674, 792
MspA1I CMGCKG 1 cut(s) 371
MspI CCGG 4 cut(s) 125, 512, 735, 872
MspR9I CCNGG 2 cut(s) 362, 735
MvaI CCWGG 1 cut(s) 362
MvnI CGCG 1 cut(s) 517
MwoI GCNNNNNNNGC 4 cut(s) 134, 284, 329, 377
NciI CCSGG 1 cut(s) 735
NdeI CATATG 2 cut(s) 466, 880
NdeII GATC 4 cut(s) 160, 208, 577, 742
NheI GCTAGC 1 cut(s) 859
NlaIII CATG 2 cut(s) 457, 558
NlaIV GGNNCC 9 cut(s) 82, 177, 225, 273, 318, 366, 472, 521, 579
NmuCI GTSAC 1 cut(s) 874
NsiI ATGCAT 2 cut(s) 455, 685
NspI RCATGY 1 cut(s) 457
PaqCI CACCTGC 1 cut(s) 238
PfeI GAWTC 2 cut(s) 98, 728
PfoI TCCNGGA 1 cut(s) 733
PkrI GCNGC 3 cut(s) 53, 325, 373
PleI GAGTC 2 cut(s) 127, 313
PpsI GAGTC 2 cut(s) 127, 313
Psp6I CCWGG 1 cut(s) 360
PspGI CCWGG 1 cut(s) 360
PspN4I GGNNCC 9 cut(s) 82, 177, 225, 273, 318, 366, 472, 521, 579
PsrI GAACNNNNNNTAC 2 cut(s) 463, 495
PstI CTGCAG 2 cut(s) 56, 64
PstNI CAGNNNCTG 2 cut(s) 51, 217
PsuI RGATCY 1 cut(s) 577
PvuII CAGCTG 1 cut(s) 371
RsaI GTAC 5 cut(s) 58, 82, 478, 521, 885
RsaNI GTAC 5 cut(s) 57, 81, 477, 520, 884
SaqAI TTAA 3 cut(s) 606, 674, 792
SatI GCNGC 3 cut(s) 52, 324, 372
Sau3AI GATC 4 cut(s) 160, 208, 577, 742
ScaI AGTACT 2 cut(s) 58, 478
SchI GAGTC 2 cut(s) 128, 314
ScrFI CCNGG 2 cut(s) 362, 735
SduI GDGCHC 1 cut(s) 493
SfaNI GCATC 3 cut(s) 379, 440, 670
SfcI CTRYAG 3 cut(s) 52, 60, 693
SpeI ACTAGT 1 cut(s) 485
Sse9I AATT 2 cut(s) 808, 937
SsiI CCGC 7 cut(s) 93, 141, 237, 285, 324, 378, 517
SspI AATATT 2 cut(s) 538, 834
SspMI CTAG 7 cut(s) 71, 206, 329, 486, 720, 848, 860
StyD4I CCNGG 2 cut(s) 360, 733
TaaI ACNGT 3 cut(s) 106, 193, 443
TaiI ACGT 1 cut(s) 858
TasI AATT 2 cut(s) 808, 937
TatI WGTACW 2 cut(s) 56, 476
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 2 cut(s) 98, 728
Tru1I TTAA 3 cut(s) 606, 674, 792
Tru9I TTAA 3 cut(s) 606, 674, 792
TscAI CASTG 1 cut(s) 109
TseFI GTSAC 1 cut(s) 874
TseI GCWGC 2 cut(s) 51, 371
Tsp45I GTSAC 1 cut(s) 874
TspDTI ATGAA 4 cut(s) 16, 17, 698, 829
TspGWI ACGGA 3 cut(s) 284, 442, 590
TspRI CASTG 1 cut(s) 109
VneI GTGCAC 1 cut(s) 489
XapI RAATTY 1 cut(s) 808
XceI RCATGY 1 cut(s) 457
XmiI GTMKAC 1 cut(s) 348
XspI CTAG 7 cut(s) 71, 206, 329, 486, 720, 848, 860
ZrmI AGTACT 2 cut(s) 58, 478
Zsp2I ATGCAT 2 cut(s) 455, 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.