Rroxscaffold_3G00234620

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0031995
Physical Location & Seq
Reverse (-)
21117472 .. 21119762
2291 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00234620.1

Sequence Viewer

Length: 765 bp
ATGAGGTCCTCGATCGAGATACAAGACGTTCTCGGACTAAGTTATGATGCTCTAGGAGCAGATTTGAAAGGACTATTTCTTGATATTGCTTGCTTCTTCAAAGGTGAGCAAGTAGACCGTGTGAAACCAATACTAGAAGCTTGCTACGACCTTAAATCAGCAAGTGATACGCTTGATCTGTCATATAACAATTTTGTTAGTCTTCCTGCTTGCATCACCAAATTTGTCAACTTGCGGACGCTTTTCTTATACTATTGCAGGAGACTCCGAGGAATTCCGGAGCTTCCCCCAAAGGTTAAACTAGTCATAGAGGGGAAATCACTGGAGAGATTCTCGAAGTTGACAAATCCTGGAGCTGTGATGGAGCTGGCGCCGCCGTCAGTTGAAGATGATTGTGAATTACTAGACATAGAGGCAGTGTCGCCACCATTAGTTGAAGTTGATTTTGAATTACTAGACATAGAGGCGGCGTCGCTGTCAATAGAAGATGGTGATCAAGAAGAGCCAACTCCGTCGTCACGGCTTTCTTTGTCTTCCGAACCTCCAAAACGACACCACTCCCCGGAAGAACCATCAACAATGATTCCTCCAAAACACCATCGTTGGATGGCATTTTGGAGGAAATACTTAACTACTCCACAATGCGCTACCGGGCAAGCATCAGTGATCCCTGACCTGCATAGCTCCCATGACTCAACTACTCCACAGCTCCAACACCATGAGGAACCAACTAAATTCTCTGACTATGGAGCCAACAAAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

254

Amino Acids

28.43

Weight (kDa)

4.98

Isoelectric Point (pI)

60.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031995)

Species Orthologous Gene IDs
rosa_roxburghii Rroxscaffold_3G00234620
rosa_samantha Rh7DG370200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 684
AccB1I GGYRCC 1 cut(s) 370
AccI GTMKAC 1 cut(s) 114
AccIII TCCGGA 1 cut(s) 277
AciI CCGC 3 cut(s) 235, 374, 467
AclWI GGATC 1 cut(s) 661
AcsI RAATTY 3 cut(s) 221, 273, 734
AcyI GRCGYC 2 cut(s) 371, 470
AfiI CCNNNNNNNGG 1 cut(s) 562
AgsI TTSAA 5 cut(s) 67, 100, 386, 437, 449
AhlI ACTAGT 1 cut(s) 301
AjnI CCWGG 1 cut(s) 349
AluBI AGCT 6 cut(s) 140, 283, 356, 367, 684, 709
AluI AGCT 6 cut(s) 140, 283, 356, 367, 684, 709
Alw26I GTCTC 1 cut(s) 256
AlwI GGATC 1 cut(s) 661
Aor13HI TCCGGA 1 cut(s) 277
ApoI RAATTY 3 cut(s) 221, 273, 734
AspLEI GCGC 2 cut(s) 373, 647
AspS9I GGNCC 1 cut(s) 6
AsuC2I CCSGG 2 cut(s) 563, 652
AsuHPI GGTGA 3 cut(s) 116, 208, 503
AvaII GGWCC 1 cut(s) 6
BanI GGYRCC 1 cut(s) 370
BbsI GAAGAC 2 cut(s) 194, 525
BccI CCATC 5 cut(s) 355, 482, 580, 601, 606
BceAI ACGGC 2 cut(s) 361, 536
BciT130I CCWGG 1 cut(s) 351
BclI TGATCA 1 cut(s) 493
BcnI CCSGG 2 cut(s) 563, 652
BcoDI GTCTC 1 cut(s) 256
BcuI ACTAGT 1 cut(s) 301
BfaI CTAG 6 cut(s) 53, 134, 302, 404, 455, 763
BfoI RGCGCY 1 cut(s) 374
BfuAI ACCTGC 1 cut(s) 684
BisI GCNGC 2 cut(s) 374, 468
BlsI GCNGC 2 cut(s) 375, 469
Bme1390I CCNGG 3 cut(s) 351, 563, 652
Bme18I GGWCC 1 cut(s) 6
BmgT120I GGNCC 1 cut(s) 6
BmiI GGNNCC 3 cut(s) 372, 726, 751
BmrFI CCNGG 3 cut(s) 351, 563, 652
BmsI GCATC 3 cut(s) 37, 222, 668
BpiI GAAGAC 2 cut(s) 194, 525
BplI GAGNNNNNCTC 2 cut(s) 317, 349
BpmI CTGGAG 2 cut(s) 344, 372
BpuMI CCSGG 2 cut(s) 563, 652
BsaBI GATNNNNATC 1 cut(s) 492
BsaHI GRCGYC 2 cut(s) 371, 470
BsaJI CCNNGG 2 cut(s) 268, 561
BsaWI WCCGGW 1 cut(s) 277
Bsc4I CCNNNNNNNGG 1 cut(s) 562
Bse1I ACTGG 1 cut(s) 327
Bse8I GATNNNNATC 1 cut(s) 492
BseAI TCCGGA 1 cut(s) 277
BseBI CCWGG 1 cut(s) 351
BseDI CCNNGG 2 cut(s) 268, 561
BseGI GGATG 1 cut(s) 612
BseJI GATNNNNATC 1 cut(s) 492
BseLI CCNNNNNNNGG 1 cut(s) 562
BseNI ACTGG 1 cut(s) 327
Bsh1285I CGRYCG 1 cut(s) 15
BshNI GGYRCC 1 cut(s) 370
BsiEI CGRYCG 1 cut(s) 15
BsiSI CCGG 3 cut(s) 278, 563, 651
BslI CCNNNNNNNGG 1 cut(s) 562
BsmAI GTCTC 1 cut(s) 256
Bsp13I TCCGGA 1 cut(s) 277
Bsp143I GATC 4 cut(s) 12, 175, 493, 666
BspACI CCGC 3 cut(s) 235, 374, 467
BspEI TCCGGA 1 cut(s) 277
BspLI GGNNCC 3 cut(s) 372, 726, 751
BspMI ACCTGC 1 cut(s) 684
BspPI GGATC 1 cut(s) 661
BspQI GCTCTTC 1 cut(s) 495
BspT107I GGYRCC 1 cut(s) 370
BsrI ACTGG 1 cut(s) 327
BssECI CCNNGG 2 cut(s) 268, 561
BssMI GATC 4 cut(s) 12, 175, 493, 666
BssNI GRCGYC 2 cut(s) 371, 470
Bst2UI CCWGG 1 cut(s) 351
Bst4CI ACNGT 1 cut(s) 119
Bst6I CTCTTC 1 cut(s) 495
BstACI GRCGYC 2 cut(s) 371, 470
BstC8I GCNNGC 5 cut(s) 91, 142, 211, 369, 657
BstDEI CTNAG 1 cut(s) 38
BstF5I GGATG 1 cut(s) 612
BstH2I RGCGCY 1 cut(s) 374
BstHHI GCGC 2 cut(s) 373, 647
BstKTI GATC 4 cut(s) 15, 178, 496, 669
BstMAI GTCTC 1 cut(s) 256
BstMBI GATC 4 cut(s) 12, 175, 493, 666
BstMCI CGRYCG 1 cut(s) 15
BstMWI GCNNNNNNNGC 2 cut(s) 56, 373
BstNI CCWGG 1 cut(s) 351
BstSCI CCNGG 3 cut(s) 349, 561, 650
BstV2I GAAGAC 2 cut(s) 194, 525
BtsCI GGATG 1 cut(s) 612
BtsI GCAGTG 1 cut(s) 423
BtsIMutI CAGTG 3 cut(s) 320, 423, 669
BveI ACCTGC 1 cut(s) 684
Cac8I GCNNGC 5 cut(s) 91, 142, 211, 369, 657
CfoI GCGC 2 cut(s) 373, 647
Cfr13I GGNCC 1 cut(s) 6
CseI GACGC 2 cut(s) 247, 459
CviAII CATG 2 cut(s) 689, 719
CviJI RGCY 9 cut(s) 140, 283, 356, 367, 505, 523, 684, 709, 752
CviKI_1 RGCY 9 cut(s) 140, 283, 356, 367, 505, 523, 684, 709, 752
DdeI CTNAG 1 cut(s) 38
DinI GGCGCC 1 cut(s) 372
DpnI GATC 4 cut(s) 14, 177, 495, 668
DpnII GATC 4 cut(s) 12, 175, 493, 666
Eam1104I CTCTTC 1 cut(s) 495
EarI CTCTTC 1 cut(s) 495
Eco47I GGWCC 1 cut(s) 6
EcoO109I RGGNCCY 1 cut(s) 6
EcoRI GAATTC 1 cut(s) 273
EcoRII CCWGG 1 cut(s) 349
EgeI GGCGCC 1 cut(s) 372
EheI GGCGCC 1 cut(s) 372
FaeI CATG 2 cut(s) 692, 722
FatI CATG 2 cut(s) 688, 718
FbaI TGATCA 1 cut(s) 493
FblI GTMKAC 1 cut(s) 114
Fnu4HI GCNGC 2 cut(s) 374, 468
FokI GGATG 1 cut(s) 619
Fsp4HI GCNGC 2 cut(s) 374, 468
FspBI CTAG 6 cut(s) 53, 134, 302, 404, 455, 763
GlaI GCGC 2 cut(s) 372, 646
GluI GCNGC 2 cut(s) 374, 468
GsuI CTGGAG 2 cut(s) 344, 372
HaeII RGCGCY 1 cut(s) 374
HapII CCGG 3 cut(s) 278, 563, 651
HgaI GACGC 2 cut(s) 247, 459
HhaI GCGC 2 cut(s) 373, 647
Hin1I GRCGYC 2 cut(s) 371, 470
Hin1II CATG 2 cut(s) 692, 722
Hin6I GCGC 2 cut(s) 371, 645
HinP1I GCGC 2 cut(s) 371, 645
HincII GTYRAC 2 cut(s) 229, 342
HindII GTYRAC 2 cut(s) 229, 342
HindIII AAGCTT 1 cut(s) 138
HinfI GANTC 4 cut(s) 264, 330, 583, 692
HpaII CCGG 3 cut(s) 278, 563, 651
HphI GGTGA 3 cut(s) 116, 208, 503
Hpy166II GTNNAC 3 cut(s) 115, 229, 342
Hpy188I TCNGA 4 cut(s) 35, 269, 538, 742
Hpy188III TCNNGA 5 cut(s) 16, 80, 278, 334, 497
Hpy8I GTNNAC 3 cut(s) 115, 229, 342
Hpy99I CGWCG 2 cut(s) 475, 517
HpyCH4III ACNGT 1 cut(s) 119
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 3 cut(s) 213, 258, 679
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 373
HpyF3I CTNAG 1 cut(s) 38
HpySE526I ACGT 1 cut(s) 27
Hsp92I GRCGYC 2 cut(s) 371, 470
Hsp92II CATG 2 cut(s) 692, 722
HspAI GCGC 2 cut(s) 371, 645
KasI GGCGCC 1 cut(s) 370
Kpn2I TCCGGA 1 cut(s) 277
Ksp22I TGATCA 1 cut(s) 493
Kzo9I GATC 4 cut(s) 12, 175, 493, 666
LguI GCTCTTC 1 cut(s) 495
LmnI GCTCC 7 cut(s) 56, 280, 353, 364, 689, 714, 749
LweI GCATC 3 cut(s) 37, 222, 668
MaeI CTAG 6 cut(s) 53, 134, 302, 404, 455, 763
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 516
MalI GATC 4 cut(s) 14, 177, 495, 668
MboI GATC 4 cut(s) 12, 175, 493, 666
MboII GAAGA 7 cut(s) 88, 194, 398, 497, 512, 525, 578
MluCI AATT 6 cut(s) 190, 221, 273, 398, 449, 734
Mly113I GGCGCC 1 cut(s) 371
MlyI GAGTC 2 cut(s) 258, 686
MmeI TCCRAC 2 cut(s) 584, 736
MnlI CCTC 9 cut(s) 19, 263, 304, 406, 457, 552, 597, 612, 715
MroI TCCGGA 1 cut(s) 277
MseI TTAA 3 cut(s) 153, 297, 629
MspI CCGG 3 cut(s) 278, 563, 651
MspR9I CCNGG 3 cut(s) 351, 563, 652
MvaI CCWGG 1 cut(s) 351
MwoI GCNNNNNNNGC 2 cut(s) 56, 373
NarI GGCGCC 1 cut(s) 371
NciI CCSGG 2 cut(s) 563, 652
NdeII GATC 4 cut(s) 12, 175, 493, 666
NlaIII CATG 2 cut(s) 692, 722
NlaIV GGNNCC 3 cut(s) 372, 726, 751
NmuCI GTSAC 1 cut(s) 516
PciSI GCTCTTC 1 cut(s) 495
PfeI GAWTC 2 cut(s) 330, 583
PfoI TCCNGGA 1 cut(s) 349
PkrI GCNGC 2 cut(s) 375, 469
Ple19I CGATCG 1 cut(s) 15
PleI GAGTC 2 cut(s) 258, 686
PluTI GGCGCC 1 cut(s) 374
PpsI GAGTC 2 cut(s) 258, 686
PpuMI RGGWCCY 1 cut(s) 6
Psp5II RGGWCCY 1 cut(s) 6
Psp6I CCWGG 1 cut(s) 349
PspGI CCWGG 1 cut(s) 349
PspN4I GGNNCC 3 cut(s) 372, 726, 751
PspPI GGNCC 1 cut(s) 6
PspPPI RGGWCCY 1 cut(s) 6
PvuI CGATCG 1 cut(s) 15
SapI GCTCTTC 1 cut(s) 495
SaqAI TTAA 3 cut(s) 153, 297, 629
SatI GCNGC 2 cut(s) 374, 468
Sau3AI GATC 4 cut(s) 12, 175, 493, 666
Sau96I GGNCC 1 cut(s) 6
SchI GAGTC 2 cut(s) 258, 686
ScrFI CCNGG 3 cut(s) 351, 563, 652
SfaNI GCATC 3 cut(s) 37, 222, 668
SfoI GGCGCC 1 cut(s) 372
SinI GGWCC 1 cut(s) 6
SpeI ACTAGT 1 cut(s) 301
Sse9I AATT 6 cut(s) 190, 221, 273, 398, 449, 734
SsiI CCGC 3 cut(s) 235, 374, 467
SspDI GGCGCC 1 cut(s) 370
SspMI CTAG 6 cut(s) 53, 134, 302, 404, 455, 763
StyD4I CCNGG 3 cut(s) 349, 561, 650
TaaI ACNGT 1 cut(s) 119
TaiI ACGT 1 cut(s) 30
TaqI TCGA 3 cut(s) 11, 15, 335
TasI AATT 6 cut(s) 190, 221, 273, 398, 449, 734
TauI GCSGC 2 cut(s) 376, 470
TfiI GAWTC 2 cut(s) 330, 583
Tru1I TTAA 3 cut(s) 153, 297, 629
Tru9I TTAA 3 cut(s) 153, 297, 629
TscAI CASTG 3 cut(s) 327, 423, 669
TseFI GTSAC 1 cut(s) 516
Tsp45I GTSAC 1 cut(s) 516
TspGWI ACGGA 1 cut(s) 501
TspRI CASTG 3 cut(s) 327, 423, 669
VpaK11BI GGWCC 1 cut(s) 6
XapI RAATTY 3 cut(s) 221, 273, 734
XmiI GTMKAC 1 cut(s) 114
XspI CTAG 6 cut(s) 53, 134, 302, 404, 455, 763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.