Rroxscaffold_4G00281500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0024654
Physical Location & Seq
Forward (+)
3786421 .. 3787266
846 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00281500.1

Sequence Viewer

Length: 333 bp
ATGTCGAGCCTCCGCTTGCCGCCGGACTCGTTGATGAAGAGTTCGAAGTCGACCTTGATTGGGCTTGAGGTTTCGGGTGAGGCTCAAAAGTGCTTAATCTCAACTCTCGTTGTTGCCATTGTCTTCCTAGCAATAATTCAAAGCTCCGATTGTCGACCTATTAGTACTGCTACTGCTCAAGCATCCAATACAGAACAAAGGTCGAGTTACAGCAACAGGTATGCTCCAATGTTCCTAAGCGCCATGGAGTCCATCAGAAGGAACAAGATCAACCTGAGTCACACAGTTTCGCGCCGAATGGTCCCCGCAGGACCCAATCCGCTTCACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

110

Amino Acids

11.98

Weight (kDa)

10.03

Isoelectric Point (pI)

63.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024654)

Species Orthologous Gene IDs
pyrus_communis pycom07g24490
rosa_roxburghii Rroxscaffold_4G00281500
rosa_samantha Rh1AG418100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 50, 154
AccII CGCG 1 cut(s) 292
AciI CCGC 4 cut(s) 13, 20, 306, 320
AfaI GTAC 1 cut(s) 166
AfiI CCNNNNNNNGG 2 cut(s) 60, 258
AgsI TTSAA 1 cut(s) 140
AluBI AGCT 1 cut(s) 144
AluI AGCT 1 cut(s) 144
AspLEI GCGC 2 cut(s) 242, 294
AspS9I GGNCC 2 cut(s) 301, 311
AsuHPI GGTGA 1 cut(s) 89
AsuII TTCGAA 1 cut(s) 44
AvaII GGWCC 2 cut(s) 301, 311
BbsI GAAGAC 1 cut(s) 115
BccI CCATC 1 cut(s) 260
BfaI CTAG 1 cut(s) 128
BfoI RGCGCY 1 cut(s) 243
BisI GCNGC 1 cut(s) 20
BlsI GCNGC 1 cut(s) 21
BmcAI AGTACT 1 cut(s) 166
Bme18I GGWCC 2 cut(s) 301, 311
BmgT120I GGNCC 2 cut(s) 301, 311
BmiI GGNNCC 2 cut(s) 303, 313
BmsI GCATC 1 cut(s) 191
BpiI GAAGAC 1 cut(s) 115
Bpu10I CCTNAGC 1 cut(s) 236
Bpu14I TTCGAA 1 cut(s) 44
BpuEI CTTGAG 2 cut(s) 86, 162
BsaJI CCNNGG 1 cut(s) 243
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 258
BseDI CCNNGG 1 cut(s) 243
BseGI GGATG 1 cut(s) 182
BseLI CCNNNNNNNGG 2 cut(s) 60, 258
BseMII CTCAG 1 cut(s) 266
Bsh1236I CGCG 1 cut(s) 292
BsiSI CCGG 1 cut(s) 23
BslFI GGGAC 1 cut(s) 287
BslI CCNNNNNNNGG 2 cut(s) 60, 258
BsmFI GGGAC 1 cut(s) 287
Bsp119I TTCGAA 1 cut(s) 44
Bsp143I GATC 1 cut(s) 267
Bsp19I CCATGG 1 cut(s) 243
BspACI CCGC 4 cut(s) 13, 20, 306, 320
BspCNI CTCAG 1 cut(s) 267
BspFNI CGCG 1 cut(s) 292
BspLI GGNNCC 2 cut(s) 303, 313
BspT104I TTCGAA 1 cut(s) 44
BssECI CCNNGG 1 cut(s) 243
BssMI GATC 1 cut(s) 267
BssT1I CCWWGG 1 cut(s) 243
Bst4CI ACNGT 1 cut(s) 286
Bst6I CTCTTC 1 cut(s) 32
BstBI TTCGAA 1 cut(s) 44
BstC8I GCNNGC 1 cut(s) 17
BstDEI CTNAG 2 cut(s) 236, 275
BstDSI CCRYGG 1 cut(s) 243
BstF5I GGATG 1 cut(s) 182
BstFNI CGCG 1 cut(s) 292
BstH2I RGCGCY 1 cut(s) 243
BstHHI GCGC 2 cut(s) 242, 294
BstKTI GATC 1 cut(s) 270
BstMBI GATC 1 cut(s) 267
BstUI CGCG 1 cut(s) 292
BstV2I GAAGAC 1 cut(s) 115
BtgI CCRYGG 1 cut(s) 243
BtsCI GGATG 1 cut(s) 182
Cac8I GCNNGC 1 cut(s) 17
CfoI GCGC 2 cut(s) 242, 294
Cfr13I GGNCC 2 cut(s) 301, 311
Csp6I GTAC 1 cut(s) 165
CviAII CATG 1 cut(s) 244
CviJI RGCY 4 cut(s) 9, 64, 83, 144
CviKI_1 RGCY 4 cut(s) 9, 64, 83, 144
CviQI GTAC 1 cut(s) 165
DdeI CTNAG 2 cut(s) 236, 275
DpnI GATC 1 cut(s) 269
DpnII GATC 1 cut(s) 267
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Eco130I CCWWGG 1 cut(s) 243
Eco47I GGWCC 2 cut(s) 301, 311
EcoO109I RGGNCCY 1 cut(s) 311
EcoT14I CCWWGG 1 cut(s) 243
ErhI CCWWGG 1 cut(s) 243
FaeI CATG 1 cut(s) 247
FaiI YATR 2 cut(s) 222, 245
FalI AAGNNNNNCTT 2 cut(s) 38, 70
FaqI GGGAC 1 cut(s) 287
FatI CATG 1 cut(s) 243
FauI CCCGC 1 cut(s) 313
FblI GTMKAC 2 cut(s) 50, 154
Fnu4HI GCNGC 1 cut(s) 20
FokI GGATG 1 cut(s) 169
Fsp4HI GCNGC 1 cut(s) 20
FspBI CTAG 1 cut(s) 128
GlaI GCGC 2 cut(s) 241, 293
GluI GCNGC 1 cut(s) 20
HaeII RGCGCY 1 cut(s) 243
HapII CCGG 1 cut(s) 23
HhaI GCGC 2 cut(s) 242, 294
Hin1II CATG 1 cut(s) 247
Hin6I GCGC 2 cut(s) 240, 292
HinP1I GCGC 2 cut(s) 240, 292
HincII GTYRAC 2 cut(s) 51, 155
HindII GTYRAC 2 cut(s) 51, 155
HinfI GANTC 3 cut(s) 26, 248, 277
HpaII CCGG 1 cut(s) 23
HphI GGTGA 1 cut(s) 89
Hpy166II GTNNAC 2 cut(s) 51, 155
Hpy188I TCNGA 2 cut(s) 148, 257
Hpy8I GTNNAC 2 cut(s) 51, 155
HpyAV CCTTC 1 cut(s) 252
HpyCH4III ACNGT 1 cut(s) 286
HpyF3I CTNAG 2 cut(s) 236, 275
Hsp92II CATG 1 cut(s) 247
HspAI GCGC 2 cut(s) 240, 292
Kzo9I GATC 1 cut(s) 267
LmnI GCTCC 2 cut(s) 149, 229
LpnPI CCDG 4 cut(s) 36, 202, 287, 294
LweI GCATC 1 cut(s) 191
MaeI CTAG 1 cut(s) 128
MaeIII GTNAC 2 cut(s) 206, 278
MalI GATC 1 cut(s) 269
MboI GATC 1 cut(s) 267
MboII GAAGA 2 cut(s) 49, 115
MfeI CAATTG 1 cut(s) 328
MluCI AATT 2 cut(s) 135, 328
MlyI GAGTC 3 cut(s) 20, 257, 286
MnlI CCTC 3 cut(s) 20, 61, 73
MseI TTAA 1 cut(s) 95
MspI CCGG 1 cut(s) 23
MunI CAATTG 1 cut(s) 328
MvnI CGCG 1 cut(s) 292
NcoI CCATGG 1 cut(s) 243
NdeII GATC 1 cut(s) 267
NlaIII CATG 1 cut(s) 247
NlaIV GGNNCC 2 cut(s) 303, 313
NmuCI GTSAC 1 cut(s) 278
NspV TTCGAA 1 cut(s) 44
PkrI GCNGC 1 cut(s) 21
PleI GAGTC 3 cut(s) 20, 256, 285
PpsI GAGTC 3 cut(s) 20, 256, 285
PpuMI RGGWCCY 1 cut(s) 311
Psp5II RGGWCCY 1 cut(s) 311
PspN4I GGNNCC 2 cut(s) 303, 313
PspPI GGNCC 2 cut(s) 301, 311
PspPPI RGGWCCY 1 cut(s) 311
RsaI GTAC 1 cut(s) 166
RsaNI GTAC 1 cut(s) 165
SalI GTCGAC 2 cut(s) 49, 153
SaqAI TTAA 1 cut(s) 95
SatI GCNGC 1 cut(s) 20
Sau3AI GATC 1 cut(s) 267
Sau96I GGNCC 2 cut(s) 301, 311
ScaI AGTACT 1 cut(s) 166
SchI GAGTC 3 cut(s) 20, 257, 286
SetI ASST 7 cut(s) 56, 72, 146, 160, 203, 221, 276
SfaNI GCATC 1 cut(s) 191
SfuI TTCGAA 1 cut(s) 44
SinI GGWCC 2 cut(s) 301, 311
SmlI CTYRAG 2 cut(s) 65, 177
SmoI CTYRAG 2 cut(s) 65, 177
Sse9I AATT 2 cut(s) 135, 328
SsiI CCGC 4 cut(s) 13, 20, 306, 320
SspMI CTAG 1 cut(s) 128
StyI CCWWGG 1 cut(s) 243
TaaI ACNGT 1 cut(s) 286
TaqI TCGA 5 cut(s) 5, 44, 50, 154, 203
TasI AATT 2 cut(s) 135, 328
TatI WGTACW 1 cut(s) 164
TauI GCSGC 1 cut(s) 22
Tru1I TTAA 1 cut(s) 95
Tru9I TTAA 1 cut(s) 95
TseFI GTSAC 1 cut(s) 278
Tsp45I GTSAC 1 cut(s) 278
TspDTI ATGAA 1 cut(s) 50
VpaK11BI GGWCC 2 cut(s) 301, 311
XmiI GTMKAC 2 cut(s) 50, 154
XspI CTAG 1 cut(s) 128
ZrmI AGTACT 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.