Rroxscaffold_4G00285550

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0015356
Physical Location & Seq
Reverse (-)
6830386 .. 6834001
3616 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00285550.1

Sequence Viewer

Length: 1386 bp
ATGGCATTTGCAACAAGGCCTCTACCTCACAGATTGCTTTCTTCTACCCTCTCTCTATACACCATTGCTTTCGGTTCCGGGGGAACAAAGCCTAATATATCCACCTTCGGGGCAGACCAGTTTGATGATTTTAACCCCCACGAGAAAAGGATCAAGGCCTCATTCTTCAACTGGTGGATGTTCAGCTCTTTCTTAGGTGCTCTGATAGCAACCCTTGGCCTTGTCTACATCCAAGAAAACGTGGGATGGGGGTTAGGGTATGGTATCCCCACATTTGGTCTAGTAGTATCCTTATTAATCTTCTATATTGGGACACCAATGTATCGGCACAAAGTTAGCAAGAACAAGAGCCCAGCTAGGGACATCATCCAAATTCCCGTTGCTGCCTTTAGAAATCGGAAGCTTCCCCTCCCAAATGATCTGTCTGAGCTCCATGAGTATGAGCCACAACATTACATTGACAGCGGAAAACGCCAGGTCTATCACACTCCAACTTTCAGGTTCTTGGACAAGGCTGCTATAAAAAATGCCAACACGGATGCCTCATCAAGACCACCATGCACAGTGACTCAAGTTGAAGGAACAAAGCTGATTTTGGGAATGGGCATGATATGGTTTGTGACCCTAATTCCAAGCACCATTTGGGCACAAATCAACACTCTCTTTGTCAAGCAAGGCACCACACTAGACAGAAGCTTGGGCCCGAATCACTTCCATATCCCAGCAGCTTCTCTAGGGAGCTTCGTCACCCTCTCCATGCTTCTCTCCGTGCCCATGTATGACCGTTACTTCGTGCCCTTTATGCGTGGCAGAACCGGAAACCCTAGGGGAATCACACTCCTCCAAAGGCTTGGGATTGGATTCGTCATCCAAGTCATAGCCATTGCAATTGCCTATGCGGTTGAGGTTCGTCGCATGCGTGTCATTCGAGTCCACCATGTTTCGGGGCCTAAAGAGGTCGTCCCCATGAGCATATTCTGGTTACTCCCTCAGTACACACTACTCGGGGTAGCCGATGTGTTCAACGCCATTGGATTGCTGGAGTTTTTCTACGATCAGTCCCCAGAGGACATGCAGAGTCTCGGCACAACTTTCTTCACAAGTGGGATTGGTATTGGTAACTTCTTGAACAGCTTTTTAGTGACGATGGTGGATAAAATTACGGGGAGGAATGGGGGTAAAAGTTGGATTGGGAATAACTTGAATGACTGTCACTTGGATTATTACTACGGATTTCTTCTGGTCATATCTTCCCTAAACCTGGTGGCGTTTTTGTGGGCTTCAAGCAAATATATATACAAGAAGGAATCTGTATACGTGAAGGAAGGGTGCATTGATTTGATTGAAGCCAAAGCAATGACCACACCTCTAGGAATATGTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009605 GO:0009607 GO:0009624 GO:0043207 GO:0050896 GO:0051704 GO:0051707
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

461

Amino Acids

51.56

Weight (kDa)

9.35

Isoelectric Point (pI)

33.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 10 - 397 1.6e-98 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015356)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40460
fragaria_vesca FvH4_7g25560
malus_domestica MD07G1230600.v1.1
prunus_persica Prupe.2G260700_v2.0.a1 Prupe.2G260700_v2.0.a1
pyrus_communis pycom07g20820
rosa_chinensis RchiOBHm_Chr1g0371561
rosa_laevigata RLG00000026952
rosa_multiflora Rmu_sc0000809.1_g000010
rosa_roxburghii Rroxscaffold_4G00285550
rosa_rugosa Rorug01G0365400
rosa_samantha Rh1AG375300 Rh1BG338800 Rh1CG352000 Rh1DG369900
rosa_wichuraiana Rw1G033020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 677
AccI GTMKAC 2 cut(s) 225, 1314
AciI CCGC 2 cut(s) 465, 899
AclWI GGATC 1 cut(s) 158
AcsI RAATTY 1 cut(s) 372
AfaI GTAC 1 cut(s) 995
AfiI CCNNNNNNNGG 5 cut(s) 108, 275, 358, 943, 1261
AgsI TTSAA 7 cut(s) 169, 578, 1024, 1129, 1204, 1284, 1346
AjnI CCWGG 2 cut(s) 474, 1260
AluBI AGCT 9 cut(s) 186, 356, 403, 430, 589, 696, 728, 741, 1134
AluI AGCT 9 cut(s) 186, 356, 403, 430, 589, 696, 728, 741, 1134
Alw21I GWGCWC 2 cut(s) 202, 432
Alw26I GTCTC 1 cut(s) 1085
AlwI GGATC 1 cut(s) 158
Ama87I CYCGRG 1 cut(s) 1004
AoxI GGCC 5 cut(s) 17, 156, 217, 700, 947
ApaI GGGCCC 1 cut(s) 704
ApeKI GCWGC 3 cut(s) 383, 515, 725
ApoI RAATTY 1 cut(s) 372
ArsI GACNNNNNNTTYG 4 cut(s) 407, 439, 773, 805
AseI ATTAAT 1 cut(s) 296
Asp700I GAANNNNTTC 1 cut(s) 710
AspA2I CCTAGG 1 cut(s) 824
AspS9I GGNCC 3 cut(s) 700, 701, 947
AsuC2I CCSGG 1 cut(s) 79
AsuHPI GGTGA 1 cut(s) 739
AvaI CYCGRG 1 cut(s) 1004
AvrII CCTAGG 1 cut(s) 824
BaeGI GKGCMC 4 cut(s) 649, 704, 774, 798
BaeI ACNNNNGTAYC 2 cut(s) 305, 338
BanI GGYRCC 1 cut(s) 677
BanII GRGCYC 3 cut(s) 353, 432, 704
BauI CACGAG 1 cut(s) 140
Bbv12I GWGCWC 2 cut(s) 202, 432
BbvI GCAGC 3 cut(s) 370, 502, 737
BccI CCATC 2 cut(s) 240, 1141
BciT130I CCWGG 2 cut(s) 476, 1262
BciVI GTATCC 2 cut(s) 275, 298
BcnI CCSGG 1 cut(s) 79
BcoDI GTCTC 1 cut(s) 1085
BfaI CTAG 6 cut(s) 281, 357, 686, 734, 825, 1370
BfuI GTATCC 2 cut(s) 275, 298
BisI GCNGC 3 cut(s) 384, 516, 726
BlnI CCTAGG 1 cut(s) 824
BlsI GCNGC 3 cut(s) 385, 517, 727
Bme1390I CCNGG 3 cut(s) 79, 476, 1262
BmeT110I CYCGRG 1 cut(s) 1004
BmgT120I GGNCC 3 cut(s) 700, 701, 947
BmiI GGNNCC 4 cut(s) 76, 679, 702, 948
BmrFI CCNGG 3 cut(s) 79, 476, 1262
BmsI GCATC 1 cut(s) 529
BpmI CTGGAG 1 cut(s) 1061
BpuEI CTTGAG 1 cut(s) 555
BpuMI CCSGG 1 cut(s) 79
BsaAI YACGTR 1 cut(s) 1318
BsaJI CCNNGG 3 cut(s) 78, 214, 824
BsaWI WCCGGW 1 cut(s) 815
Bsc4I CCNNNNNNNGG 5 cut(s) 108, 275, 358, 943, 1261
Bse1I ACTGG 2 cut(s) 118, 176
Bse3DI GCAATG 3 cut(s) 63, 882, 1362
BseBI CCWGG 2 cut(s) 476, 1262
BseDI CCNNGG 3 cut(s) 78, 214, 824
BseGI GGATG 6 cut(s) 183, 228, 251, 366, 544, 867
BseLI CCNNNNNNNGG 5 cut(s) 108, 275, 358, 943, 1261
BseMI GCAATG 3 cut(s) 63, 882, 1362
BseMII CTCAG 2 cut(s) 417, 1004
BseNI ACTGG 2 cut(s) 118, 176
BseRI GAGGAG 1 cut(s) 830
BseSI GKGCMC 4 cut(s) 649, 704, 774, 798
BseXI GCAGC 3 cut(s) 370, 502, 737
BseYI CCCAGC 2 cut(s) 352, 721
BshFI GGCC 5 cut(s) 19, 158, 219, 702, 949
BshNI GGYRCC 1 cut(s) 677
BsiHKAI GWGCWC 2 cut(s) 202, 432
BsiHKCI CYCGRG 1 cut(s) 1004
BsiSI CCGG 2 cut(s) 78, 816
BslFI GGGAC 4 cut(s) 325, 374, 947, 1045
BslI CCNNNNNNNGG 5 cut(s) 108, 275, 358, 943, 1261
BsmAI GTCTC 1 cut(s) 1085
BsmFI GGGAC 4 cut(s) 325, 374, 947, 1045
BsnI GGCC 5 cut(s) 19, 158, 219, 702, 949
BsoBI CYCGRG 1 cut(s) 1004
Bsp120I GGGCCC 1 cut(s) 700
Bsp1286I GDGCHC 7 cut(s) 202, 353, 432, 649, 704, 774, 798
Bsp143I GATC 3 cut(s) 150, 418, 1054
BspACI CCGC 2 cut(s) 465, 899
BspANI GGCC 5 cut(s) 19, 158, 219, 702, 949
BspCNI CTCAG 2 cut(s) 418, 1003
BspLI GGNNCC 4 cut(s) 76, 679, 702, 948
BspPI GGATC 1 cut(s) 158
BspT107I GGYRCC 1 cut(s) 677
BsrDI GCAATG 3 cut(s) 63, 882, 1362
BsrI ACTGG 2 cut(s) 118, 176
BssECI CCNNGG 3 cut(s) 78, 214, 824
BssMI GATC 3 cut(s) 150, 418, 1054
BssNAI GTATAC 1 cut(s) 1315
BssSI CACGAG 1 cut(s) 140
BssT1I CCWWGG 2 cut(s) 214, 824
Bst1107I GTATAC 1 cut(s) 1315
Bst2BI CACGAG 1 cut(s) 140
Bst2UI CCWGG 2 cut(s) 476, 1262
Bst4CI ACNGT 3 cut(s) 565, 785, 1211
BstBAI YACGTR 1 cut(s) 1318
BstC8I GCNNGC 1 cut(s) 917
BstDEI CTNAG 3 cut(s) 193, 426, 990
BstF5I GGATG 6 cut(s) 183, 228, 251, 366, 544, 867
BstKTI GATC 3 cut(s) 153, 421, 1057
BstMAI GTCTC 1 cut(s) 1085
BstMBI GATC 3 cut(s) 150, 418, 1054
BstMWI GCNNNNNNNGC 3 cut(s) 206, 471, 802
BstNI CCWGG 2 cut(s) 476, 1262
BstNSI RCATGY 2 cut(s) 919, 1075
BstSCI CCNGG 3 cut(s) 77, 474, 1260
BstSLI GKGCMC 4 cut(s) 649, 704, 774, 798
BstV1I GCAGC 3 cut(s) 370, 502, 737
BstXI CCANNNNNNTGG 1 cut(s) 851
BstZ17I GTATAC 1 cut(s) 1315
BsuI GTATCC 2 cut(s) 275, 298
BsuRI GGCC 5 cut(s) 19, 158, 219, 702, 949
BtsCI GGATG 6 cut(s) 183, 228, 251, 366, 544, 867
BtsIMutI CAGTG 1 cut(s) 570
Cac8I GCNNGC 1 cut(s) 917
Cfr13I GGNCC 3 cut(s) 700, 701, 947
CsiI ACCWGGT 1 cut(s) 1260
Csp6I GTAC 1 cut(s) 994
CviAII CATG 9 cut(s) 434, 558, 607, 757, 775, 916, 938, 967, 1072
CviQI GTAC 1 cut(s) 994
DdeI CTNAG 3 cut(s) 193, 426, 990
DpnI GATC 3 cut(s) 152, 420, 1056
DpnII GATC 3 cut(s) 150, 418, 1054
Ecl136II GAGCTC 1 cut(s) 430
Eco130I CCWWGG 2 cut(s) 214, 824
Eco147I AGGCCT 2 cut(s) 19, 158
Eco24I GRGCYC 3 cut(s) 353, 432, 704
Eco53kI GAGCTC 1 cut(s) 430
Eco88I CYCGRG 1 cut(s) 1004
EcoICRI GAGCTC 1 cut(s) 430
EcoO109I RGGNCCY 1 cut(s) 947
EcoRII CCWGG 2 cut(s) 474, 1260
EcoT14I CCWWGG 2 cut(s) 214, 824
EcoT38I GRGCYC 3 cut(s) 353, 432, 704
ErhI CCWWGG 2 cut(s) 214, 824
FaeI CATG 9 cut(s) 437, 561, 610, 760, 778, 919, 941, 970, 1075
FaqI GGGAC 4 cut(s) 325, 374, 947, 1045
FatI CATG 9 cut(s) 433, 557, 606, 756, 774, 915, 937, 966, 1071
FblI GTMKAC 2 cut(s) 225, 1314
Fnu4HI GCNGC 3 cut(s) 384, 516, 726
FokI GGATG 6 cut(s) 190, 215, 258, 353, 551, 854
FriOI GRGCYC 3 cut(s) 353, 432, 704
Fsp4HI GCNGC 3 cut(s) 384, 516, 726
FspBI CTAG 6 cut(s) 281, 357, 686, 734, 825, 1370
GluI GCNGC 3 cut(s) 384, 516, 726
GsaI CCCAGC 2 cut(s) 356, 725
GsuI CTGGAG 1 cut(s) 1061
HaeIII GGCC 5 cut(s) 19, 158, 219, 702, 949
HapII CCGG 2 cut(s) 78, 816
Hin1II CATG 9 cut(s) 437, 561, 610, 760, 778, 919, 941, 970, 1075
HindIII AAGCTT 2 cut(s) 401, 694
HinfI GANTC 7 cut(s) 568, 706, 831, 861, 930, 1078, 1307
HpaII CCGG 2 cut(s) 78, 816
HphI GGTGA 1 cut(s) 739
Hpy166II GTNNAC 4 cut(s) 226, 934, 996, 1315
Hpy188I TCNGA 3 cut(s) 204, 399, 427
Hpy188III TCNNGA 2 cut(s) 549, 1126
Hpy8I GTNNAC 4 cut(s) 226, 934, 996, 1315
Hpy99I CGWCG 1 cut(s) 915
HpyAV CCTTC 5 cut(s) 115, 572, 1297, 1315, 1319
HpyCH4III ACNGT 3 cut(s) 565, 785, 1211
HpyCH4IV ACGT 2 cut(s) 240, 1317
HpyCH4V TGCA 5 cut(s) 11, 561, 887, 1075, 1332
HpyF10VI GCNNNNNNNGC 3 cut(s) 206, 471, 802
HpyF3I CTNAG 3 cut(s) 193, 426, 990
HpySE526I ACGT 2 cut(s) 240, 1317
Hsp92II CATG 9 cut(s) 437, 561, 610, 760, 778, 919, 941, 970, 1075
Kzo9I GATC 3 cut(s) 150, 418, 1054
LmnI GCTCC 2 cut(s) 435, 738
Lsp1109I GCAGC 3 cut(s) 370, 502, 737
LweI GCATC 1 cut(s) 529
MabI ACCWGGT 1 cut(s) 1260
MaeI CTAG 6 cut(s) 281, 357, 686, 734, 825, 1370
MaeII ACGT 2 cut(s) 240, 1317
MaeIII GTNAC 8 cut(s) 565, 619, 745, 785, 981, 1118, 1141, 1211
MalI GATC 3 cut(s) 152, 420, 1056
MboI GATC 3 cut(s) 150, 418, 1054
MboII GAAGA 6 cut(s) 33, 157, 292, 1087, 1229, 1242
MfeI CAATTG 1 cut(s) 888
MhlI GDGCHC 7 cut(s) 202, 353, 432, 649, 704, 774, 798
MluCI AATT 5 cut(s) 372, 627, 888, 1158, 1381
MlyI GAGTC 3 cut(s) 562, 939, 1087
MmeI TCCRAC 2 cut(s) 515, 1166
MroXI GAANNNNTTC 1 cut(s) 710
MseI TTAA 3 cut(s) 132, 296, 1384
MslI CAYNNNNRTG 1 cut(s) 438
MspA1I CMGCKG 1 cut(s) 465
MspI CCGG 2 cut(s) 78, 816
MspR9I CCNGG 3 cut(s) 79, 476, 1262
MunI CAATTG 1 cut(s) 888
MvaI CCWGG 2 cut(s) 476, 1262
MwoI GCNNNNNNNGC 3 cut(s) 206, 471, 802
NciI CCSGG 1 cut(s) 79
NdeII GATC 3 cut(s) 150, 418, 1054
NlaIII CATG 9 cut(s) 437, 561, 610, 760, 778, 919, 941, 970, 1075
NlaIV GGNNCC 4 cut(s) 76, 679, 702, 948
NmeAIII GCCGAG 1 cut(s) 1062
NmuCI GTSAC 5 cut(s) 565, 619, 745, 1141, 1211
NspI RCATGY 2 cut(s) 919, 1075
PaeI GCATGC 1 cut(s) 919
PceI AGGCCT 2 cut(s) 19, 158
PcsI WCGNNNNNNNCGW 2 cut(s) 916, 1011
PdmI GAANNNNTTC 1 cut(s) 710
PfeI GAWTC 4 cut(s) 706, 831, 861, 1307
PkrI GCNGC 3 cut(s) 385, 517, 727
PleI GAGTC 3 cut(s) 562, 938, 1086
PpsI GAGTC 3 cut(s) 562, 938, 1086
Ppu21I YACGTR 1 cut(s) 1318
PshBI ATTAAT 1 cut(s) 296
Psp124BI GAGCTC 1 cut(s) 432
Psp6I CCWGG 2 cut(s) 474, 1260
PspFI CCCAGC 2 cut(s) 352, 721
PspGI CCWGG 2 cut(s) 474, 1260
PspN4I GGNNCC 4 cut(s) 76, 679, 702, 948
PspOMI GGGCCC 1 cut(s) 700
PspPI GGNCC 3 cut(s) 700, 701, 947
RsaI GTAC 1 cut(s) 995
RsaNI GTAC 1 cut(s) 994
RseI CAYNNNNRTG 1 cut(s) 438
SacI GAGCTC 1 cut(s) 432
SaqAI TTAA 3 cut(s) 132, 296, 1384
SatI GCNGC 3 cut(s) 384, 516, 726
Sau3AI GATC 3 cut(s) 150, 418, 1054
Sau96I GGNCC 3 cut(s) 700, 701, 947
SchI GAGTC 3 cut(s) 562, 939, 1087
ScrFI CCNGG 3 cut(s) 79, 476, 1262
SduI GDGCHC 7 cut(s) 202, 353, 432, 649, 704, 774, 798
SexAI ACCWGGT 1 cut(s) 1260
SfaNI GCATC 1 cut(s) 529
SmiMI CAYNNNNRTG 1 cut(s) 438
SmlI CTYRAG 1 cut(s) 570
SmoI CTYRAG 1 cut(s) 570
SphI GCATGC 1 cut(s) 919
Sse9I AATT 5 cut(s) 372, 627, 888, 1158, 1381
SseBI AGGCCT 2 cut(s) 19, 158
SsiI CCGC 2 cut(s) 465, 899
SspMI CTAG 6 cut(s) 281, 357, 686, 734, 825, 1370
SstI GAGCTC 1 cut(s) 432
StuI AGGCCT 2 cut(s) 19, 158
StyD4I CCNGG 3 cut(s) 77, 474, 1260
StyI CCWWGG 2 cut(s) 214, 824
TaaI ACNGT 3 cut(s) 565, 785, 1211
TaiI ACGT 2 cut(s) 243, 1320
TaqI TCGA 1 cut(s) 928
TasI AATT 5 cut(s) 372, 627, 888, 1158, 1381
TatI WGTACW 1 cut(s) 993
TfiI GAWTC 4 cut(s) 706, 831, 861, 1307
Tru1I TTAA 3 cut(s) 132, 296, 1384
Tru9I TTAA 3 cut(s) 132, 296, 1384
TscAI CASTG 1 cut(s) 570
TseFI GTSAC 5 cut(s) 565, 619, 745, 1141, 1211
TseI GCWGC 3 cut(s) 383, 515, 725
Tsp45I GTSAC 5 cut(s) 565, 619, 745, 1141, 1211
TspGWI ACGGA 3 cut(s) 551, 757, 1245
TspRI CASTG 1 cut(s) 570
VspI ATTAAT 1 cut(s) 296
XapI RAATTY 1 cut(s) 372
XceI RCATGY 2 cut(s) 919, 1075
XcmI CCANNNNNNNNNTGG 2 cut(s) 639, 1036
XmaJI CCTAGG 1 cut(s) 824
XmiI GTMKAC 2 cut(s) 225, 1314
XmnI GAANNNNTTC 1 cut(s) 710
XspI CTAG 6 cut(s) 281, 357, 686, 734, 825, 1370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.