Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
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| Species | Orthologous Gene IDs |
|---|---|
| rosa_multiflora | Rmu_sc0004889.1_g000018 |
| rosa_roxburghii | Rroxscaffold_4G00302430 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccIII | TCCGGA | 1 cut(s) | 205 |
| AclWI | GGATC | 2 cut(s) | 197, 210 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 138 |
| AgsI | TTSAA | 1 cut(s) | 51 |
| AluBI | AGCT | 2 cut(s) | 129, 211 |
| AluI | AGCT | 2 cut(s) | 129, 211 |
| Alw21I | GWGCWC | 1 cut(s) | 131 |
| AlwI | GGATC | 2 cut(s) | 197, 210 |
| Aor13HI | TCCGGA | 1 cut(s) | 205 |
| AspS9I | GGNCC | 2 cut(s) | 193, 216 |
| AvaII | GGWCC | 2 cut(s) | 193, 216 |
| BamHI | GGATCC | 1 cut(s) | 202 |
| BanII | GRGCYC | 1 cut(s) | 131 |
| BauI | CACGAG | 1 cut(s) | 197 |
| BbsI | GAAGAC | 1 cut(s) | 8 |
| Bbv12I | GWGCWC | 1 cut(s) | 131 |
| BccI | CCATC | 3 cut(s) | 92, 100, 218 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 178, 212 |
| BclI | TGATCA | 1 cut(s) | 268 |
| Bme18I | GGWCC | 2 cut(s) | 193, 216 |
| BmgT120I | GGNCC | 2 cut(s) | 193, 216 |
| BmiI | GGNNCC | 1 cut(s) | 204 |
| BpiI | GAAGAC | 1 cut(s) | 8 |
| Bpu10I | CCTNAGC | 1 cut(s) | 212 |
| BpuEI | CTTGAG | 1 cut(s) | 157 |
| Bsa29I | ATCGAT | 1 cut(s) | 247 |
| BsaJI | CCNNGG | 1 cut(s) | 219 |
| BsaWI | WCCGGW | 2 cut(s) | 103, 205 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 138 |
| Bse118I | RCCGGY | 1 cut(s) | 115 |
| BseAI | TCCGGA | 1 cut(s) | 205 |
| BseCI | ATCGAT | 1 cut(s) | 247 |
| BseDI | CCNNGG | 1 cut(s) | 219 |
| BseGI | GGATG | 1 cut(s) | 229 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 138 |
| BseRI | GAGGAG | 1 cut(s) | 57 |
| BshVI | ATCGAT | 1 cut(s) | 247 |
| BsiHKAI | GWGCWC | 1 cut(s) | 131 |
| BsiSI | CCGG | 3 cut(s) | 104, 116, 206 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 138 |
| Bsp1286I | GDGCHC | 1 cut(s) | 131 |
| Bsp13I | TCCGGA | 1 cut(s) | 205 |
| Bsp143I | GATC | 4 cut(s) | 202, 233, 244, 268 |
| Bsp19I | CCATGG | 1 cut(s) | 219 |
| BspDI | ATCGAT | 1 cut(s) | 247 |
| BspEI | TCCGGA | 1 cut(s) | 205 |
| BspLI | GGNNCC | 1 cut(s) | 204 |
| BspPI | GGATC | 2 cut(s) | 197, 210 |
| BsrFI | RCCGGY | 1 cut(s) | 115 |
| BssAI | RCCGGY | 1 cut(s) | 115 |
| BssECI | CCNNGG | 1 cut(s) | 219 |
| BssMI | GATC | 4 cut(s) | 202, 233, 244, 268 |
| BssSI | CACGAG | 1 cut(s) | 197 |
| BssT1I | CCWWGG | 1 cut(s) | 219 |
| Bst2BI | CACGAG | 1 cut(s) | 197 |
| Bst4CI | ACNGT | 1 cut(s) | 193 |
| BstC8I | GCNNGC | 2 cut(s) | 41, 187 |
| BstDEI | CTNAG | 1 cut(s) | 212 |
| BstDSI | CCRYGG | 1 cut(s) | 219 |
| BstF5I | GGATG | 1 cut(s) | 229 |
| BstKTI | GATC | 4 cut(s) | 205, 236, 247, 271 |
| BstMBI | GATC | 4 cut(s) | 202, 233, 244, 268 |
| BstNSI | RCATGY | 1 cut(s) | 43 |
| BstV2I | GAAGAC | 1 cut(s) | 8 |
| BstX2I | RGATCY | 1 cut(s) | 202 |
| BstYI | RGATCY | 1 cut(s) | 202 |
| Bsu15I | ATCGAT | 1 cut(s) | 247 |
| BsuTUI | ATCGAT | 1 cut(s) | 247 |
| BtgI | CCRYGG | 1 cut(s) | 219 |
| BtsCI | GGATG | 1 cut(s) | 229 |
| Cac8I | GCNNGC | 2 cut(s) | 41, 187 |
| Cfr10I | RCCGGY | 1 cut(s) | 115 |
| Cfr13I | GGNCC | 2 cut(s) | 193, 216 |
| ClaI | ATCGAT | 1 cut(s) | 247 |
| CviAII | CATG | 5 cut(s) | 26, 36, 40, 220, 237 |
| CviJI | RGCY | 3 cut(s) | 129, 164, 211 |
| CviKI_1 | RGCY | 3 cut(s) | 129, 164, 211 |
| DdeI | CTNAG | 1 cut(s) | 212 |
| DpnI | GATC | 4 cut(s) | 204, 235, 246, 270 |
| DpnII | GATC | 4 cut(s) | 202, 233, 244, 268 |
| DraI | TTTAAA | 1 cut(s) | 76 |
| Ecl136II | GAGCTC | 1 cut(s) | 129 |
| Eco130I | CCWWGG | 1 cut(s) | 219 |
| Eco24I | GRGCYC | 1 cut(s) | 131 |
| Eco47I | GGWCC | 2 cut(s) | 193, 216 |
| Eco53kI | GAGCTC | 1 cut(s) | 129 |
| EcoICRI | GAGCTC | 1 cut(s) | 129 |
| EcoT14I | CCWWGG | 1 cut(s) | 219 |
| EcoT22I | ATGCAT | 1 cut(s) | 41 |
| EcoT38I | GRGCYC | 1 cut(s) | 131 |
| ErhI | CCWWGG | 1 cut(s) | 219 |
| FaeI | CATG | 5 cut(s) | 29, 39, 43, 223, 240 |
| FaiI | YATR | 7 cut(s) | 11, 27, 37, 41, 180, 221, 238 |
| FatI | CATG | 5 cut(s) | 25, 35, 39, 219, 236 |
| FbaI | TGATCA | 1 cut(s) | 268 |
| FokI | GGATG | 1 cut(s) | 236 |
| FriOI | GRGCYC | 1 cut(s) | 131 |
| HapII | CCGG | 3 cut(s) | 104, 116, 206 |
| Hin1II | CATG | 5 cut(s) | 29, 39, 43, 223, 240 |
| HinfI | GANTC | 3 cut(s) | 121, 249, 279 |
| HpaII | CCGG | 3 cut(s) | 104, 116, 206 |
| Hpy188I | TCNGA | 1 cut(s) | 126 |
| Hpy188III | TCNNGA | 1 cut(s) | 206 |
| HpyCH4III | ACNGT | 1 cut(s) | 193 |
| HpyCH4V | TGCA | 2 cut(s) | 39, 185 |
| HpyF3I | CTNAG | 1 cut(s) | 212 |
| Hsp92II | CATG | 5 cut(s) | 29, 39, 43, 223, 240 |
| Kpn2I | TCCGGA | 1 cut(s) | 205 |
| Ksp22I | TGATCA | 1 cut(s) | 268 |
| Kzo9I | GATC | 4 cut(s) | 202, 233, 244, 268 |
| LmnI | GCTCC | 2 cut(s) | 134, 208 |
| LpnPI | CCDG | 4 cut(s) | 117, 129, 156, 219 |
| MalI | GATC | 4 cut(s) | 204, 235, 246, 270 |
| MboI | GATC | 4 cut(s) | 202, 233, 244, 268 |
| MboII | GAAGA | 1 cut(s) | 8 |
| MflI | RGATCY | 1 cut(s) | 202 |
| MhlI | GDGCHC | 1 cut(s) | 131 |
| MlyI | GAGTC | 1 cut(s) | 115 |
| MmeI | TCCRAC | 1 cut(s) | 156 |
| MnlI | CCTC | 4 cut(s) | 75, 78, 132, 206 |
| Mph1103I | ATGCAT | 1 cut(s) | 41 |
| MroI | TCCGGA | 1 cut(s) | 205 |
| MseI | TTAA | 1 cut(s) | 75 |
| MspI | CCGG | 3 cut(s) | 104, 116, 206 |
| NcoI | CCATGG | 1 cut(s) | 219 |
| NdeII | GATC | 4 cut(s) | 202, 233, 244, 268 |
| NlaIII | CATG | 5 cut(s) | 29, 39, 43, 223, 240 |
| NlaIV | GGNNCC | 1 cut(s) | 204 |
| NsiI | ATGCAT | 1 cut(s) | 41 |
| NspI | RCATGY | 1 cut(s) | 43 |
| PaeI | GCATGC | 1 cut(s) | 43 |
| PfeI | GAWTC | 2 cut(s) | 249, 279 |
| PleI | GAGTC | 1 cut(s) | 115 |
| PpsI | GAGTC | 1 cut(s) | 115 |
| Psp124BI | GAGCTC | 1 cut(s) | 131 |
| PspN4I | GGNNCC | 1 cut(s) | 204 |
| PspPI | GGNCC | 2 cut(s) | 193, 216 |
| PsuI | RGATCY | 1 cut(s) | 202 |
| SacI | GAGCTC | 1 cut(s) | 131 |
| SaqAI | TTAA | 1 cut(s) | 75 |
| Sau3AI | GATC | 4 cut(s) | 202, 233, 244, 268 |
| Sau96I | GGNCC | 2 cut(s) | 193, 216 |
| SchI | GAGTC | 1 cut(s) | 115 |
| SduI | GDGCHC | 1 cut(s) | 131 |
| SetI | ASST | 5 cut(s) | 8, 16, 131, 213, 277 |
| SgrAI | CRCCGGYG | 1 cut(s) | 115 |
| SinI | GGWCC | 2 cut(s) | 193, 216 |
| SmlI | CTYRAG | 1 cut(s) | 136 |
| SmoI | CTYRAG | 1 cut(s) | 136 |
| SphI | GCATGC | 1 cut(s) | 43 |
| SstI | GAGCTC | 1 cut(s) | 131 |
| StyI | CCWWGG | 1 cut(s) | 219 |
| TaaI | ACNGT | 1 cut(s) | 193 |
| TaqI | TCGA | 1 cut(s) | 247 |
| TfiI | GAWTC | 2 cut(s) | 249, 279 |
| Tru1I | TTAA | 1 cut(s) | 75 |
| Tru9I | TTAA | 1 cut(s) | 75 |
| VpaK11BI | GGWCC | 2 cut(s) | 193, 216 |
| XceI | RCATGY | 1 cut(s) | 43 |
| Zsp2I | ATGCAT | 1 cut(s) | 41 |