Rroxscaffold_4G00317060

Apoptosis inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0029581
Physical Location & Seq
Reverse (-)
42133232 .. 42139002
5771 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00317060.1

Sequence Viewer

Length: 1167 bp
ATGGCGACGGCGGCAGCGGCAGACGAGGCCATGCACATTGAGAAGCCCTACCTGTTCGGCGAGCGACTCAACAAGTCTATGGATAGCAAGTCCCAGAACATGAAAGACTACCCGGGGTTTACCAATGCGGCAAAAATGAGCCTGAAGAACAGGCAGCTCGTTGCACAGCTTATTCAGAGGGTTCGAGTGCAAGCTATCCGGGGGCATCCCCTTTTCTTGAAGGACACCCGTGAATATATCTTGAAGATTGTACGCATTCTTGTGCAATCCCTACAAGCTGATGAGTTTGTCGAGCGTGATGCTGCGCGTAAGGCTCTTATGGCCCTTTTGAGGAGCCTGGATGTGAAAGCTTCTTTTACAGCCTTGTTCAAGCACATCGGGAGTGTTGATGACCCAAGCCAAGATGATGTTATTCGTGAGAAATTCCTAAGCTTTATCAGAGATAAGGTATTTCCTATTAAAGCTGAAGTTCTGAAGCTTCAGGAGCAAATGGAGAGGCATATTACTGACTTGATAAAAAAGATTTTAGAAGATGTAACTGGAGCAGAATTTAGAATGTTTATGGACTTCTTGAAAGGTTTGAACATTTTTGGGGAGAAAGCTCCGAAAGAATGCATGGTAGAACTAATTGGAACCGTTGAAGGACAAGCTGATTTAGATGCTCAGTTCAATTGCAAAACGGTTGGCATGCAGGTTTCAGATGCAGACCATATCGACAGGTTGATATCTTACCTATTTATGGCCCTGCCATTTGTTCTGCTTCCAAATGAACGAAGGCTAGAATTGCTCAAGTCCCTTGCAGAAGTTGCACCATTCACCACACCAGAAGATTCACGCCATATTCTTCCATCTGTGGTTCAGCCTGTAAAGCAAACTGAAAAGCCGAATACTACAACTCAGTTGCGAACCTGCAATGACATATTGGCAACAAAGGTACTGCTTTCAAAGGCACCTTCATTTATTGGAGACAAGAGCCTCAACTTGTCATGGAAAGAACTGCCTAAAGCTGCTGTGCCTTCTAACAGACCTGCCAAAAGATTCAAAGGGACTAGTACAGCGGCCGTTATTGGAGGTCATAAAGTGTATCTAGTGGCAGAAAGTAGGTCATCGTCTGGAAAAGAAGGTGAAGAAGGACTTATTAAGTCAGGAAATGCGCAGAAGCTATGA

Protein Analysis

388

Amino Acids

43.43

Weight (kDa)

9.17

Isoelectric Point (pI)

45.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
API5 PF05918 13 - 60 1.5e-06 Apoptosis inhibitory protein 5 (API5)
API5 PF05918 60 - 290 2.9e-68 Apoptosis inhibitory protein 5 (API5)
API5 PF05918 290 - 351 3.1e-06 Apoptosis inhibitory protein 5 (API5)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029581)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0362691
rosa_roxburghii Rroxscaffold_4G00317060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1155
Acc36I ACCTGC 3 cut(s) 682, 917, 1036
AccB1I GGYRCC 1 cut(s) 949
AccII CGCG 1 cut(s) 307
AciI CCGC 4 cut(s) 11, 17, 128, 1058
AcoI YGGCCR 1 cut(s) 1059
AcsI RAATTY 2 cut(s) 422, 548
AcuI CTGAAG 4 cut(s) 164, 464, 486, 494
AfaI GTAC 3 cut(s) 252, 936, 1054
AfiI CCNNNNNNNGG 2 cut(s) 330, 739
AgsI TTSAA 9 cut(s) 220, 244, 370, 574, 583, 641, 670, 945, 1042
AhlI ACTAGT 1 cut(s) 1049
AjnI CCWGG 1 cut(s) 336
Alw26I GTCTC 1 cut(s) 960
Ama87I CYCGRG 1 cut(s) 112
AoxI GGCC 4 cut(s) 27, 321, 741, 1059
ApeKI GCWGC 4 cut(s) 14, 154, 302, 1007
ApoI RAATTY 2 cut(s) 422, 548
AspLEI GCGC 2 cut(s) 307, 1156
AspS9I GGNCC 2 cut(s) 322, 742
AsuC2I CCSGG 3 cut(s) 113, 114, 200
AsuHPI GGTGA 2 cut(s) 808, 1136
AvaI CYCGRG 1 cut(s) 112
BanI GGYRCC 1 cut(s) 949
BbvI GCAGC 4 cut(s) 26, 166, 289, 994
BccI CCATC 1 cut(s) 856
BceAI ACGGC 2 cut(s) 24, 1046
BcgI CGANNNNNNTGC 2 cut(s) 281, 315
BciT130I CCWGG 1 cut(s) 338
BcnI CCSGG 3 cut(s) 113, 114, 200
BcoDI GTCTC 1 cut(s) 960
BcuI ACTAGT 1 cut(s) 1049
BfaI CTAG 3 cut(s) 779, 1050, 1088
BfuAI ACCTGC 3 cut(s) 682, 917, 1036
BisI GCNGC 8 cut(s) 12, 15, 18, 129, 155, 303, 1008, 1059
BlsI GCNGC 8 cut(s) 13, 16, 19, 130, 156, 304, 1009, 1060
Bme1390I CCNGG 4 cut(s) 113, 114, 200, 338
BmeT110I CYCGRG 1 cut(s) 112
BmgT120I GGNCC 2 cut(s) 322, 742
BmiI GGNNCC 3 cut(s) 335, 634, 951
BmrFI CCNGG 4 cut(s) 113, 114, 200, 338
BmsI GCATC 4 cut(s) 214, 289, 649, 691
BpmI CTGGAG 1 cut(s) 561
Bpu10I CCTNAGC 1 cut(s) 428
BpuEI CTTGAG 1 cut(s) 773
BpuMI CCSGG 3 cut(s) 113, 114, 200
BsaJI CCNNGG 3 cut(s) 112, 113, 199
Bsc4I CCNNNNNNNGG 2 cut(s) 330, 739
Bse1I ACTGG 1 cut(s) 544
Bse3DI GCAATG 1 cut(s) 919
BseBI CCWGG 1 cut(s) 338
BseDI CCNNGG 3 cut(s) 112, 113, 199
BseGI GGATG 2 cut(s) 205, 346
BseLI CCNNNNNNNGG 2 cut(s) 330, 739
BseMI GCAATG 1 cut(s) 919
BseMII CTCAG 2 cut(s) 677, 911
BseNI ACTGG 1 cut(s) 544
BseRI GAGGAG 1 cut(s) 346
BseX3I CGGCCG 1 cut(s) 1059
BseXI GCAGC 4 cut(s) 26, 166, 289, 994
Bsh1236I CGCG 1 cut(s) 307
Bsh1285I CGRYCG 1 cut(s) 1062
BshFI GGCC 4 cut(s) 29, 323, 743, 1061
BshNI GGYRCC 1 cut(s) 949
BsiEI CGRYCG 1 cut(s) 1062
BsiHKCI CYCGRG 1 cut(s) 112
BsiSI CCGG 2 cut(s) 113, 199
BslFI GGGAC 3 cut(s) 76, 778, 1060
BslI CCNNNNNNNGG 2 cut(s) 330, 739
BsmAI GTCTC 1 cut(s) 960
BsmFI GGGAC 3 cut(s) 76, 778, 1060
BsmI GAATGC 2 cut(s) 255, 617
BsnI GGCC 4 cut(s) 29, 323, 743, 1061
BsoBI CYCGRG 1 cut(s) 112
BspACI CCGC 4 cut(s) 11, 17, 128, 1058
BspANI GGCC 4 cut(s) 29, 323, 743, 1061
BspCNI CTCAG 2 cut(s) 676, 910
BspFNI CGCG 1 cut(s) 307
BspLI GGNNCC 3 cut(s) 335, 634, 951
BspMI ACCTGC 3 cut(s) 682, 917, 1036
BspT107I GGYRCC 1 cut(s) 949
BsrDI GCAATG 1 cut(s) 919
BsrI ACTGG 1 cut(s) 544
BssECI CCNNGG 3 cut(s) 112, 113, 199
Bst2UI CCWGG 1 cut(s) 338
Bst4CI ACNGT 2 cut(s) 637, 682
BstAPI GCANNNNNTGC 1 cut(s) 806
BstC8I GCNNGC 3 cut(s) 62, 192, 689
BstDEI CTNAG 3 cut(s) 428, 663, 897
BstF5I GGATG 2 cut(s) 205, 346
BstFNI CGCG 1 cut(s) 307
BstHHI GCGC 2 cut(s) 307, 1156
BstMAI GTCTC 1 cut(s) 960
BstMCI CGRYCG 1 cut(s) 1062
BstMWI GCNNNNNNNGC 9 cut(s) 11, 17, 26, 311, 320, 484, 784, 806, 868
BstNI CCWGG 1 cut(s) 338
BstNSI RCATGY 1 cut(s) 691
BstSCI CCNGG 4 cut(s) 111, 112, 198, 336
BstUI CGCG 1 cut(s) 307
BstV1I GCAGC 4 cut(s) 26, 166, 289, 994
BstZI CGGCCG 1 cut(s) 1059
BsuRI GGCC 4 cut(s) 29, 323, 743, 1061
BtsCI GGATG 2 cut(s) 205, 346
BveI ACCTGC 3 cut(s) 682, 917, 1036
Cac8I GCNNGC 3 cut(s) 62, 192, 689
CfoI GCGC 2 cut(s) 307, 1156
Cfr13I GGNCC 2 cut(s) 322, 742
Cfr9I CCCGGG 1 cut(s) 112
Csp6I GTAC 3 cut(s) 251, 935, 1053
CviAII CATG 5 cut(s) 31, 100, 616, 688, 987
CviQI GTAC 3 cut(s) 251, 935, 1053
DdeI CTNAG 3 cut(s) 428, 663, 897
EaeI YGGCCR 1 cut(s) 1059
EagI CGGCCG 1 cut(s) 1059
EclXI CGGCCG 1 cut(s) 1059
Eco32I GATATC 1 cut(s) 726
Eco52I CGGCCG 1 cut(s) 1059
Eco57I CTGAAG 4 cut(s) 164, 464, 486, 494
Eco88I CYCGRG 1 cut(s) 112
EcoRII CCWGG 1 cut(s) 336
EcoRV GATATC 1 cut(s) 726
EcoT22I ATGCAT 1 cut(s) 617
FaeI CATG 5 cut(s) 34, 103, 619, 691, 990
FalI AAGNNNNNCTT 2 cut(s) 1119, 1151
FaqI GGGAC 3 cut(s) 76, 778, 1060
FatI CATG 5 cut(s) 30, 99, 615, 687, 986
Fnu4HI GCNGC 8 cut(s) 12, 15, 18, 129, 155, 303, 1008, 1059
FokI GGATG 2 cut(s) 192, 353
Fsp4HI GCNGC 8 cut(s) 12, 15, 18, 129, 155, 303, 1008, 1059
FspBI CTAG 3 cut(s) 779, 1050, 1088
FspI TGCGCA 1 cut(s) 1155
GlaI GCGC 2 cut(s) 306, 1155
GluI GCNGC 8 cut(s) 12, 15, 18, 129, 155, 303, 1008, 1059
GsuI CTGGAG 1 cut(s) 561
HaeIII GGCC 4 cut(s) 29, 323, 743, 1061
HapII CCGG 2 cut(s) 113, 199
HhaI GCGC 2 cut(s) 307, 1156
Hin1II CATG 5 cut(s) 34, 103, 619, 691, 990
Hin6I GCGC 2 cut(s) 305, 1154
HinP1I GCGC 2 cut(s) 305, 1154
HindIII AAGCTT 3 cut(s) 348, 430, 476
HinfI GANTC 3 cut(s) 66, 830, 1038
HpaII CCGG 2 cut(s) 113, 199
HphI GGTGA 2 cut(s) 808, 1136
Hpy166II GTNNAC 1 cut(s) 120
Hpy188I TCNGA 5 cut(s) 177, 440, 474, 606, 700
Hpy188III TCNNGA 8 cut(s) 217, 241, 379, 416, 482, 571, 1113, 1146
Hpy8I GTNNAC 1 cut(s) 120
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 7 cut(s) 214, 635, 768, 963, 1026, 1115, 1124
HpyCH4III ACNGT 2 cut(s) 637, 682
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 17, 26, 311, 320, 484, 784, 806, 868
HpyF3I CTNAG 3 cut(s) 428, 663, 897
Hsp92II CATG 5 cut(s) 34, 103, 619, 691, 990
HspAI GCGC 2 cut(s) 305, 1154
LmnI GCTCC 4 cut(s) 333, 484, 542, 607
Lsp1109I GCAGC 4 cut(s) 26, 166, 289, 994
LweI GCATC 4 cut(s) 214, 289, 649, 691
MaeI CTAG 3 cut(s) 779, 1050, 1088
MaeIII GTNAC 1 cut(s) 535
MboII GAAGA 6 cut(s) 157, 256, 542, 836, 839, 1139
MfeI CAATTG 1 cut(s) 670
MluCI AATT 5 cut(s) 422, 548, 627, 670, 782
MlyI GAGTC 1 cut(s) 60
MnlI CCTC 6 cut(s) 19, 171, 324, 489, 986, 1064
Mph1103I ATGCAT 1 cut(s) 617
MseI TTAA 2 cut(s) 459, 1140
MslI CAYNNNNRTG 1 cut(s) 260
MspA1I CMGCKG 2 cut(s) 17, 1058
MspI CCGG 2 cut(s) 113, 199
MspR9I CCNGG 4 cut(s) 113, 114, 200, 338
MunI CAATTG 1 cut(s) 670
Mva1269I GAATGC 2 cut(s) 255, 617
MvaI CCWGG 1 cut(s) 338
MvnI CGCG 1 cut(s) 307
MwoI GCNNNNNNNGC 9 cut(s) 11, 17, 26, 311, 320, 484, 784, 806, 868
NciI CCSGG 3 cut(s) 113, 114, 200
NlaIII CATG 5 cut(s) 34, 103, 619, 691, 990
NlaIV GGNNCC 3 cut(s) 335, 634, 951
NsbI TGCGCA 1 cut(s) 1155
NsiI ATGCAT 1 cut(s) 617
NspI RCATGY 1 cut(s) 691
PaeI GCATGC 1 cut(s) 691
PctI GAATGC 2 cut(s) 255, 617
PfeI GAWTC 2 cut(s) 830, 1038
PkrI GCNGC 8 cut(s) 13, 16, 19, 130, 156, 304, 1009, 1060
PleI GAGTC 1 cut(s) 60
PpsI GAGTC 1 cut(s) 60
Psp6I CCWGG 1 cut(s) 336
PspGI CCWGG 1 cut(s) 336
PspN4I GGNNCC 3 cut(s) 335, 634, 951
PspPI GGNCC 2 cut(s) 322, 742
RsaI GTAC 3 cut(s) 252, 936, 1054
RsaNI GTAC 3 cut(s) 251, 935, 1053
RseI CAYNNNNRTG 1 cut(s) 260
SaqAI TTAA 2 cut(s) 459, 1140
SatI GCNGC 8 cut(s) 12, 15, 18, 129, 155, 303, 1008, 1059
Sau96I GGNCC 2 cut(s) 322, 742
SchI GAGTC 1 cut(s) 60
ScrFI CCNGG 4 cut(s) 113, 114, 200, 338
SfaNI GCATC 4 cut(s) 214, 289, 649, 691
SmaI CCCGGG 1 cut(s) 114
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 1 cut(s) 788
SmoI CTYRAG 1 cut(s) 788
SpeI ACTAGT 1 cut(s) 1049
SphI GCATGC 1 cut(s) 691
Sse9I AATT 5 cut(s) 422, 548, 627, 670, 782
SsiI CCGC 4 cut(s) 11, 17, 128, 1058
SspMI CTAG 3 cut(s) 779, 1050, 1088
StyD4I CCNGG 4 cut(s) 111, 112, 198, 336
TaaI ACNGT 2 cut(s) 637, 682
TaqI TCGA 3 cut(s) 184, 291, 714
TasI AATT 5 cut(s) 422, 548, 627, 670, 782
TatI WGTACW 1 cut(s) 1052
TauI GCSGC 4 cut(s) 14, 20, 131, 1061
TfiI GAWTC 2 cut(s) 830, 1038
Tru1I TTAA 2 cut(s) 459, 1140
Tru9I TTAA 2 cut(s) 459, 1140
TseI GCWGC 4 cut(s) 14, 154, 302, 1007
TspDTI ATGAA 3 cut(s) 116, 783, 945
TspMI CCCGGG 1 cut(s) 112
XapI RAATTY 2 cut(s) 422, 548
XceI RCATGY 1 cut(s) 691
XmaI CCCGGG 1 cut(s) 112
XspI CTAG 3 cut(s) 779, 1050, 1088
Zsp2I ATGCAT 1 cut(s) 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.