Rroxscaffold_4G00325840

disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0032023
Physical Location & Seq
Forward (+)
57779745 .. 57780134
390 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_4G00325840.1

Sequence Viewer

Length: 390 bp
ATGGCTTTGGAGCGTATCAAAGATGAAGTGAGACTGGTTTCGGATGTCAAGAAAGAAATTGAACAGTTCACCGGAAACCTTGAAGCCGCTCGAGCCGAGCTTGAGGAGGCGAGCGAGGAGGCAAGTGAAGGATGCCGGCGTCGAAACCGGCCGGATAAGCTTAATGAAGTGTTCTATAAGTTGATCGACGAAGTGGATGAGTGGAACTCGGAAGTCCGAAGCAACCGGTTGAGAAACAGGAAAGGGAAGGAACGGGACGGCGGAAAGCTCTTGCTATCCGAGAGCGGCGGAAGAAGGATAAGAAGGAGGAGGCTAAGTTGGGCCACCTCGTTCGGTAGTGTGCAGCGAGAGATGGTCGTCGTTTACCATGGGAGATTGCTCAAGGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

129

Amino Acids

15.05

Weight (kDa)

9.6

Isoelectric Point (pI)

56.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 4 - 83 1.8e-07 Rx N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0032023)

Species Orthologous Gene IDs
rosa_roxburghii Rroxscaffold_3G00251360 Rroxscaffold_4G00325840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 89, 285
AciI CCGC 4 cut(s) 87, 261, 285, 288
AcoI YGGCCR 1 cut(s) 149
AcyI GRCGYC 1 cut(s) 139
AgeI ACCGGT 1 cut(s) 225
AgsI TTSAA 2 cut(s) 62, 83
AluBI AGCT 3 cut(s) 100, 160, 268
AluI AGCT 3 cut(s) 100, 160, 268
Alw26I GTCTC 1 cut(s) 25
Ama87I CYCGRG 1 cut(s) 90
AoxI GGCC 2 cut(s) 149, 321
ApeKI GCWGC 1 cut(s) 343
AsiGI ACCGGT 1 cut(s) 225
AspS9I GGNCC 1 cut(s) 321
AsuHPI GGTGA 1 cut(s) 61
AvaI CYCGRG 1 cut(s) 90
BbvI GCAGC 1 cut(s) 355
BccI CCATC 1 cut(s) 346
BceAI ACGGC 1 cut(s) 274
BcoDI GTCTC 1 cut(s) 25
BisI GCNGC 3 cut(s) 87, 286, 344
BlsI GCNGC 3 cut(s) 88, 287, 345
BmeT110I CYCGRG 1 cut(s) 90
BmgT120I GGNCC 1 cut(s) 321
BmsI GCATC 1 cut(s) 122
BplI GAGNNNNNCTC 2 cut(s) 191, 223
BpuEI CTTGAG 2 cut(s) 122, 365
BsaHI GRCGYC 1 cut(s) 139
BsaJI CCNNGG 1 cut(s) 367
BsaWI WCCGGW 2 cut(s) 71, 225
BsaXI ACNNNNNCTCC 2 cut(s) 301, 331
Bse118I RCCGGY 3 cut(s) 135, 147, 225
Bse1I ACTGG 1 cut(s) 39
BseDI CCNNGG 1 cut(s) 367
BseGI GGATG 3 cut(s) 49, 137, 202
BseNI ACTGG 1 cut(s) 39
BseRI GAGGAG 3 cut(s) 119, 131, 322
BseX3I CGGCCG 1 cut(s) 149
BseXI GCAGC 1 cut(s) 355
BsgI GTGCAG 1 cut(s) 362
Bsh1285I CGRYCG 1 cut(s) 152
BshFI GGCC 2 cut(s) 151, 323
BshTI ACCGGT 1 cut(s) 225
BsiEI CGRYCG 1 cut(s) 152
BsiHKCI CYCGRG 1 cut(s) 90
BsiSI CCGG 5 cut(s) 72, 136, 148, 152, 226
BslFI GGGAC 1 cut(s) 269
BsmAI GTCTC 1 cut(s) 25
BsmFI GGGAC 1 cut(s) 269
BsnI GGCC 2 cut(s) 151, 323
BsoBI CYCGRG 1 cut(s) 90
Bsp143I GATC 1 cut(s) 183
Bsp19I CCATGG 1 cut(s) 367
BspACI CCGC 4 cut(s) 87, 261, 285, 288
BspANI GGCC 2 cut(s) 151, 323
BsrBI CCGCTC 2 cut(s) 89, 285
BsrFI RCCGGY 3 cut(s) 135, 147, 225
BsrI ACTGG 1 cut(s) 39
BssAI RCCGGY 3 cut(s) 135, 147, 225
BssECI CCNNGG 1 cut(s) 367
BssMI GATC 1 cut(s) 183
BssNI GRCGYC 1 cut(s) 139
BssT1I CCWWGG 1 cut(s) 367
Bst4CI ACNGT 1 cut(s) 66
BstACI GRCGYC 1 cut(s) 139
BstC8I GCNNGC 2 cut(s) 112, 137
BstDEI CTNAG 1 cut(s) 314
BstDSI CCRYGG 1 cut(s) 367
BstF5I GGATG 3 cut(s) 49, 137, 202
BstKTI GATC 1 cut(s) 186
BstMAI GTCTC 1 cut(s) 25
BstMBI GATC 1 cut(s) 183
BstMCI CGRYCG 1 cut(s) 152
BstMWI GCNNNNNNNGC 2 cut(s) 92, 157
BstV1I GCAGC 1 cut(s) 355
BstZI CGGCCG 1 cut(s) 149
BsuRI GGCC 2 cut(s) 151, 323
BtgI CCRYGG 1 cut(s) 367
BtsCI GGATG 3 cut(s) 49, 137, 202
Cac8I GCNNGC 2 cut(s) 112, 137
Cfr10I RCCGGY 3 cut(s) 135, 147, 225
Cfr13I GGNCC 1 cut(s) 321
CseI GACGC 1 cut(s) 128
CspAI ACCGGT 1 cut(s) 225
CviAII CATG 1 cut(s) 368
CviJI RGCY 9 cut(s) 5, 86, 95, 100, 151, 160, 268, 313, 323
CviKI_1 RGCY 9 cut(s) 5, 86, 95, 100, 151, 160, 268, 313, 323
DdeI CTNAG 1 cut(s) 314
DpnI GATC 1 cut(s) 185
DpnII GATC 1 cut(s) 183
EaeI YGGCCR 1 cut(s) 149
EagI CGGCCG 1 cut(s) 149
EciI GGCGGA 2 cut(s) 276, 303
EclXI CGGCCG 1 cut(s) 149
Eco130I CCWWGG 1 cut(s) 367
Eco52I CGGCCG 1 cut(s) 149
Eco88I CYCGRG 1 cut(s) 90
EcoT14I CCWWGG 1 cut(s) 367
ErhI CCWWGG 1 cut(s) 367
FaeI CATG 1 cut(s) 371
FaiI YATR 2 cut(s) 177, 369
FaqI GGGAC 1 cut(s) 269
FatI CATG 1 cut(s) 367
Fnu4HI GCNGC 3 cut(s) 87, 286, 344
FokI GGATG 3 cut(s) 56, 144, 209
Fsp4HI GCNGC 3 cut(s) 87, 286, 344
GluI GCNGC 3 cut(s) 87, 286, 344
HaeIII GGCC 2 cut(s) 151, 323
HapII CCGG 5 cut(s) 72, 136, 148, 152, 226
HgaI GACGC 1 cut(s) 128
Hin1I GRCGYC 1 cut(s) 139
Hin1II CATG 1 cut(s) 371
HindIII AAGCTT 1 cut(s) 158
HpaII CCGG 5 cut(s) 72, 136, 148, 152, 226
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 2 cut(s) 69, 364
Hpy188I TCNGA 4 cut(s) 43, 211, 218, 280
Hpy188III TCNNGA 1 cut(s) 49
Hpy8I GTNNAC 2 cut(s) 69, 364
Hpy99I CGWCG 3 cut(s) 144, 191, 362
HpyAV CCTTC 4 cut(s) 122, 241, 288, 297
HpyCH4III ACNGT 1 cut(s) 66
HpyCH4V TGCA 1 cut(s) 343
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 157
HpyF3I CTNAG 1 cut(s) 314
Hsp92I GRCGYC 1 cut(s) 139
Hsp92II CATG 1 cut(s) 371
KroI GCCGGC 1 cut(s) 135
KroNI GCCGGC 1 cut(s) 137
Kzo9I GATC 1 cut(s) 183
LmnI GCTCC 1 cut(s) 10
LpnPI CCDG 7 cut(s) 20, 85, 149, 161, 165, 223, 239
Lsp1109I GCAGC 1 cut(s) 355
LweI GCATC 1 cut(s) 122
MalI GATC 1 cut(s) 185
MbiI CCGCTC 2 cut(s) 89, 285
MboI GATC 1 cut(s) 183
MboII GAAGA 1 cut(s) 303
MluCI AATT 1 cut(s) 57
MnlI CCTC 7 cut(s) 97, 100, 109, 112, 300, 303, 337
MroNI GCCGGC 1 cut(s) 135
MseI TTAA 1 cut(s) 162
MspI CCGG 5 cut(s) 72, 136, 148, 152, 226
MwoI GCNNNNNNNGC 2 cut(s) 92, 157
NaeI GCCGGC 1 cut(s) 137
NcoI CCATGG 1 cut(s) 367
NdeII GATC 1 cut(s) 183
NgoMIV GCCGGC 1 cut(s) 135
NlaIII CATG 1 cut(s) 371
NmeAIII GCCGAG 1 cut(s) 121
PaeR7I CTCGAG 1 cut(s) 90
PdiI GCCGGC 1 cut(s) 137
PinAI ACCGGT 1 cut(s) 225
PkrI GCNGC 3 cut(s) 88, 287, 345
PspPI GGNCC 1 cut(s) 321
PspXI VCTCGAGB 1 cut(s) 90
SaqAI TTAA 1 cut(s) 162
SatI GCNGC 3 cut(s) 87, 286, 344
Sau3AI GATC 1 cut(s) 183
Sau96I GGNCC 1 cut(s) 321
SetI ASST 5 cut(s) 81, 102, 162, 270, 329
SfaNI GCATC 1 cut(s) 122
Sfr274I CTCGAG 1 cut(s) 90
SlaI CTCGAG 1 cut(s) 90
SmlI CTYRAG 3 cut(s) 90, 101, 380
SmoI CTYRAG 3 cut(s) 90, 101, 380
Sse9I AATT 1 cut(s) 57
SsiI CCGC 4 cut(s) 87, 261, 285, 288
StyI CCWWGG 1 cut(s) 367
TaaI ACNGT 1 cut(s) 66
TaqI TCGA 3 cut(s) 91, 142, 186
TasI AATT 1 cut(s) 57
TauI GCSGC 2 cut(s) 89, 288
Tru1I TTAA 1 cut(s) 162
Tru9I TTAA 1 cut(s) 162
TseI GCWGC 1 cut(s) 343
TspDTI ATGAA 2 cut(s) 39, 180
XhoI CTCGAG 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.