Rroxscaffold_4G00328650

glycerol-3-phosphate dehydrogenase NAD(

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0025512
Physical Location & Seq
Forward (+)
61758411 .. 61760438
2028 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00328650.1

Sequence Viewer

Length: 489 bp
ATGAGTGCTACCGCTGATGGAGTGAAGTACTCACCCGAGTGGTTGGTTAAGCACTCGAATGTTTCTGTTGAGGAGAAGCTTGACAAGCTTAGGCTTTTGATGGGCAAAGCCGAAGGTGATCCATTATGGATAGTTGGTGTAGGGGCAGGTTCTTGGGGAAGTGTTTTTGTTGCTATGTTGCAAGACACTTATGGACACTTGAGAGAGAAGGCGGTAGTGAGAATATGGAGAAGATCTGGAAGGTCTGTGGATAGAGCCAGAGCTGAACATTTGTTTGAAGTGATCAATGCAAGGGAAGATGTGTTGTGGAGGCTTATTAGGCGGTGCGCTTACTTGAAGTATGTTGAGGGGAGATTAGGTGACCGGATACTTTTTGCGGATGAGATATTGAGAGACGGGTTATTGAATGCACTTGCTGCTTCAGTGCGATGTGCACTTTCCCGGCCGCACAAGTGGACTGCATTGGAGAAAGAATGTGTAATTTTTTGA

Protein Analysis

162

Amino Acids

18.42

Weight (kDa)

9.27

Isoelectric Point (pI)

39.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0025512)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0001543.1_g000006 Rmu_sc0030753.1_g000001
rosa_roxburghii Rroxscaffold_4G00328650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 137
AciI CCGC 5 cut(s) 12, 212, 322, 377, 446
AclWI GGATC 1 cut(s) 113
AcoI YGGCCR 1 cut(s) 443
AcuI CTGAAG 1 cut(s) 405
AfaI GTAC 1 cut(s) 29
AgsI TTSAA 3 cut(s) 278, 337, 406
AleI CACNNNNGTG 1 cut(s) 37
AluBI AGCT 3 cut(s) 79, 88, 263
AluI AGCT 3 cut(s) 79, 88, 263
Alw21I GWGCWC 1 cut(s) 436
Alw26I GTCTC 1 cut(s) 387
Alw44I GTGCAC 1 cut(s) 432
AlwI GGATC 1 cut(s) 113
Ama87I CYCGRG 1 cut(s) 35
AoxI GGCC 1 cut(s) 443
ApaLI GTGCAC 1 cut(s) 432
ApeKI GCWGC 1 cut(s) 416
AspLEI GCGC 1 cut(s) 329
AsuC2I CCSGG 1 cut(s) 442
AsuHPI GGTGA 3 cut(s) 24, 128, 371
AvaI CYCGRG 1 cut(s) 35
BaeGI GKGCMC 1 cut(s) 436
Bbv12I GWGCWC 1 cut(s) 436
BbvI GCAGC 1 cut(s) 403
BccI CCATC 2 cut(s) 11, 94
BciVI GTATCC 1 cut(s) 360
BclI TGATCA 1 cut(s) 282
BcnI CCSGG 1 cut(s) 442
BcoDI GTCTC 1 cut(s) 387
BfuAI ACCTGC 1 cut(s) 137
BfuI GTATCC 1 cut(s) 360
BglII AGATCT 1 cut(s) 233
BisI GCNGC 2 cut(s) 417, 446
BlsI GCNGC 2 cut(s) 418, 447
BmcAI AGTACT 1 cut(s) 29
Bme1390I CCNGG 1 cut(s) 442
BmeT110I CYCGRG 1 cut(s) 35
BmrFI CCNGG 1 cut(s) 442
Bpu10I CCTNAGC 1 cut(s) 89
BpuEI CTTGAG 1 cut(s) 220
BpuMI CCSGG 1 cut(s) 442
BsaWI WCCGGW 1 cut(s) 363
BsaXI ACNNNNNCTCC 2 cut(s) 343, 373
BseGI GGATG 1 cut(s) 385
BseRI GAGGAG 1 cut(s) 86
BseSI GKGCMC 1 cut(s) 436
BseX3I CGGCCG 1 cut(s) 443
BseXI GCAGC 1 cut(s) 403
Bsh1285I CGRYCG 1 cut(s) 446
BshFI GGCC 1 cut(s) 445
BsiEI CGRYCG 1 cut(s) 446
BsiHKAI GWGCWC 1 cut(s) 436
BsiHKCI CYCGRG 1 cut(s) 35
BsiSI CCGG 2 cut(s) 364, 442
BsmAI GTCTC 1 cut(s) 387
BsmBI CGTCTC 1 cut(s) 387
BsmI GAATGC 1 cut(s) 412
BsnI GGCC 1 cut(s) 445
BsoBI CYCGRG 1 cut(s) 35
Bsp1286I GDGCHC 1 cut(s) 436
Bsp143I GATC 3 cut(s) 118, 233, 282
BspACI CCGC 5 cut(s) 12, 212, 322, 377, 446
BspANI GGCC 1 cut(s) 445
BspMI ACCTGC 1 cut(s) 137
BspPI GGATC 1 cut(s) 113
BssMI GATC 3 cut(s) 118, 233, 282
BstAPI GCANNNNNTGC 1 cut(s) 416
BstDEI CTNAG 1 cut(s) 89
BstEII GGTNACC 1 cut(s) 359
BstF5I GGATG 1 cut(s) 385
BstHHI GCGC 1 cut(s) 329
BstKTI GATC 3 cut(s) 121, 236, 285
BstMAI GTCTC 1 cut(s) 387
BstMBI GATC 3 cut(s) 118, 233, 282
BstMCI CGRYCG 1 cut(s) 446
BstMWI GCNNNNNNNGC 3 cut(s) 85, 319, 416
BstPI GGTNACC 1 cut(s) 359
BstSCI CCNGG 1 cut(s) 440
BstSLI GKGCMC 1 cut(s) 436
BstV1I GCAGC 1 cut(s) 403
BstX2I RGATCY 1 cut(s) 233
BstYI RGATCY 1 cut(s) 233
BstZI CGGCCG 1 cut(s) 443
BsuI GTATCC 1 cut(s) 360
BsuRI GGCC 1 cut(s) 445
BtgZI GCGATG 1 cut(s) 442
BtsCI GGATG 1 cut(s) 385
BtsIMutI CAGTG 1 cut(s) 429
BveI ACCTGC 1 cut(s) 137
CfoI GCGC 1 cut(s) 329
Csp6I GTAC 1 cut(s) 28
CviJI RGCY 8 cut(s) 79, 88, 94, 110, 257, 263, 313, 445
CviKI_1 RGCY 8 cut(s) 79, 88, 94, 110, 257, 263, 313, 445
CviQI GTAC 1 cut(s) 28
DdeI CTNAG 1 cut(s) 89
DpnI GATC 3 cut(s) 120, 235, 284
DpnII GATC 3 cut(s) 118, 233, 282
EaeI YGGCCR 1 cut(s) 443
EagI CGGCCG 1 cut(s) 443
EclXI CGGCCG 1 cut(s) 443
Eco52I CGGCCG 1 cut(s) 443
Eco57I CTGAAG 1 cut(s) 405
Eco88I CYCGRG 1 cut(s) 35
Eco91I GGTNACC 1 cut(s) 359
EcoO65I GGTNACC 1 cut(s) 359
Esp3I CGTCTC 1 cut(s) 387
FaiI YATR 5 cut(s) 127, 176, 192, 226, 342
FbaI TGATCA 1 cut(s) 282
Fnu4HI GCNGC 2 cut(s) 417, 446
FokI GGATG 1 cut(s) 392
Fsp4HI GCNGC 2 cut(s) 417, 446
GlaI GCGC 1 cut(s) 328
GluI GCNGC 2 cut(s) 417, 446
HaeIII GGCC 1 cut(s) 445
HapII CCGG 2 cut(s) 364, 442
HhaI GCGC 1 cut(s) 329
Hin6I GCGC 1 cut(s) 327
HinP1I GCGC 1 cut(s) 327
HindIII AAGCTT 2 cut(s) 77, 86
HpaII CCGG 2 cut(s) 364, 442
HphI GGTGA 3 cut(s) 24, 128, 371
Hpy166II GTNNAC 2 cut(s) 434, 456
Hpy188III TCNNGA 1 cut(s) 237
Hpy8I GTNNAC 2 cut(s) 434, 456
HpyAV CCTTC 3 cut(s) 107, 202, 234
HpyCH4V TGCA 5 cut(s) 181, 290, 410, 434, 461
HpyF10VI GCNNNNNNNGC 3 cut(s) 85, 319, 416
HpyF3I CTNAG 1 cut(s) 89
HspAI GCGC 1 cut(s) 327
Ksp22I TGATCA 1 cut(s) 282
Kzo9I GATC 3 cut(s) 118, 233, 282
LpnPI CCDG 5 cut(s) 132, 222, 271, 377, 455
Lsp1109I GCAGC 1 cut(s) 403
MaeIII GTNAC 1 cut(s) 359
MalI GATC 3 cut(s) 120, 235, 284
MboI GATC 3 cut(s) 118, 233, 282
MboII GAAGA 2 cut(s) 243, 308
MflI RGATCY 1 cut(s) 233
MhlI GDGCHC 1 cut(s) 436
MluCI AATT 1 cut(s) 480
MnlI CCTC 3 cut(s) 64, 303, 340
MseI TTAA 1 cut(s) 48
MslI CAYNNNNRTG 2 cut(s) 37, 57
MspA1I CMGCKG 1 cut(s) 14
MspI CCGG 2 cut(s) 364, 442
MspR9I CCNGG 1 cut(s) 442
Mva1269I GAATGC 1 cut(s) 412
MwoI GCNNNNNNNGC 3 cut(s) 85, 319, 416
NciI CCSGG 1 cut(s) 442
NdeII GATC 3 cut(s) 118, 233, 282
NmuCI GTSAC 1 cut(s) 359
OliI CACNNNNGTG 1 cut(s) 37
PctI GAATGC 1 cut(s) 412
PkrI GCNGC 2 cut(s) 418, 447
PspEI GGTNACC 1 cut(s) 359
PsuI RGATCY 1 cut(s) 233
RsaI GTAC 1 cut(s) 29
RsaNI GTAC 1 cut(s) 28
RseI CAYNNNNRTG 2 cut(s) 37, 57
SaqAI TTAA 1 cut(s) 48
SatI GCNGC 2 cut(s) 417, 446
Sau3AI GATC 3 cut(s) 118, 233, 282
ScaI AGTACT 1 cut(s) 29
ScrFI CCNGG 1 cut(s) 442
SduI GDGCHC 1 cut(s) 436
SetI ASST 7 cut(s) 81, 90, 118, 151, 245, 265, 361
SmiMI CAYNNNNRTG 2 cut(s) 37, 57
SmlI CTYRAG 1 cut(s) 199
SmoI CTYRAG 1 cut(s) 199
Sse9I AATT 1 cut(s) 480
SsiI CCGC 5 cut(s) 12, 212, 322, 377, 446
StyD4I CCNGG 1 cut(s) 440
TaqI TCGA 1 cut(s) 56
TasI AATT 1 cut(s) 480
TatI WGTACW 1 cut(s) 27
TauI GCSGC 1 cut(s) 448
Tru1I TTAA 1 cut(s) 48
Tru9I TTAA 1 cut(s) 48
TscAI CASTG 1 cut(s) 429
TseFI GTSAC 1 cut(s) 359
TseI GCWGC 1 cut(s) 416
Tsp45I GTSAC 1 cut(s) 359
TspRI CASTG 1 cut(s) 429
VneI GTGCAC 1 cut(s) 432
ZrmI AGTACT 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.