Rroxscaffold_6G00393020

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0030771
Physical Location & Seq
Reverse (-)
12816202 .. 12817680
1479 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00393020.1

Sequence Viewer

Length: 738 bp
ATGGAAGAGTCAAAAATTTCTGTCATTGTGTTCTCTAAAAATTATGCTTCCACAAAATGGTGTTTAGAAGAACTAGTCAAGATCATTCAATGCAAAGAATCAAAGAACCAAATGGTATTCCCTGTTTTTTACAAGGTAGATCCATCGGATGTACGAAACCAAAACGGTAGTTTTGGTCGTGCACTTGCTCACCACGATAATTTGGAGAAGTCTCGATTAGAAGATATGTATAAACTTTTATGTGTTGGAGAAAGTGATGTACAAATGGTAGGGATATGGGGCATCGGTGGATTAAGTAAAACAACTATTGCCATTGCTGTTTACAATTCGATTGCGCACTCCATGTTCAAGATGCAATATTGGACGAAGGCTAGAGATAGCGGGTGTGTTAATTTTAGGGCACGAGGTAGCACAATTATCGAGGACCTTCCGTCAAACATTTATGCTCACTGGAGGGGCAGGCCTTGCCTACTTGGGGCAGCTGGTAGAACCCGGACATGGTGCATCGGGTTCCCACTTGTTTCCAGCCTATCCTCGAAGACGACGAGGCGATGTAATGTTGAGCTTCCCGACAAGAAAGTATATGTGACTGATCATCCGGTGAACATGCGGCTGCTCGACGAAGTCTACCCGAGGTTTCCGGATAGCGTGCCGGAATATCTCAACCGCGAGCCGAGGAGGAGGCGGGTGCCGAAGGGCAAGTGGCTCGGGGTGCTCGGGGTCCCCAAGGACCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

245

Amino Acids

27.99

Weight (kDa)

9.44

Isoelectric Point (pI)

37.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 2 - 70 2.7e-25 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030771)

Species Orthologous Gene IDs
rosa_laevigata RLG00000018983
rosa_roxburghii Rroxscaffold_6G00393020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 336
AccB1I GGYRCC 1 cut(s) 688
AccB7I CCANNNNNTGG 1 cut(s) 57
AccI GTMKAC 1 cut(s) 627
AccII CGCG 1 cut(s) 669
AccIII TCCGGA 1 cut(s) 640
AciI CCGC 4 cut(s) 381, 610, 667, 685
AclWI GGATC 1 cut(s) 134
AcsI RAATTY 1 cut(s) 15
AfaI GTAC 2 cut(s) 153, 261
AfiI CCNNNNNNNGG 3 cut(s) 57, 475, 498
AgsI TTSAA 2 cut(s) 89, 349
AhdI GACNNNNNGTC 1 cut(s) 430
AhlI ACTAGT 1 cut(s) 73
AluBI AGCT 2 cut(s) 482, 565
AluI AGCT 2 cut(s) 482, 565
Alw21I GWGCWC 2 cut(s) 184, 717
Alw26I GTCTC 1 cut(s) 216
Alw44I GTGCAC 1 cut(s) 180
AlwI GGATC 1 cut(s) 134
Ama87I CYCGRG 3 cut(s) 631, 707, 716
Aor13HI TCCGGA 1 cut(s) 640
AoxI GGCC 1 cut(s) 461
ApaLI GTGCAC 1 cut(s) 180
ApeKI GCWGC 2 cut(s) 479, 613
ApoI RAATTY 1 cut(s) 15
AspLEI GCGC 1 cut(s) 337
AspS9I GGNCC 3 cut(s) 424, 721, 730
AsuC2I CCSGG 1 cut(s) 493
AsuHPI GGTGA 2 cut(s) 182, 613
AvaI CYCGRG 3 cut(s) 631, 707, 716
AvaII GGWCC 3 cut(s) 424, 721, 730
BaeGI GKGCMC 2 cut(s) 184, 403
BanI GGYRCC 1 cut(s) 688
BauI CACGAG 1 cut(s) 402
BbsI GAAGAC 1 cut(s) 545
Bbv12I GWGCWC 2 cut(s) 184, 717
BbvI GCAGC 2 cut(s) 491, 600
BccI CCATC 1 cut(s) 151
BcgI CGANNNNNNTGC 4 cut(s) 400, 434, 688, 722
BclI TGATCA 1 cut(s) 592
BcnI CCSGG 1 cut(s) 493
BcoDI GTCTC 1 cut(s) 216
BcuI ACTAGT 1 cut(s) 73
BfaI CTAG 3 cut(s) 74, 372, 736
BisI GCNGC 3 cut(s) 480, 611, 614
BlsI GCNGC 3 cut(s) 481, 612, 615
Bme1390I CCNGG 1 cut(s) 493
Bme18I GGWCC 3 cut(s) 424, 721, 730
BmeRI GACNNNNNGTC 1 cut(s) 430
BmeT110I CYCGRG 3 cut(s) 631, 707, 716
BmgT120I GGNCC 3 cut(s) 424, 721, 730
BmiI GGNNCC 4 cut(s) 512, 690, 722, 723
BmrFI CCNGG 1 cut(s) 493
BmsI GCATC 3 cut(s) 291, 342, 513
BpiI GAAGAC 1 cut(s) 545
BpmI CTGGAG 1 cut(s) 472
BpuMI CCSGG 1 cut(s) 493
BsaJI CCNNGG 3 cut(s) 632, 674, 726
BsaWI WCCGGW 2 cut(s) 598, 640
Bsc4I CCNNNNNNNGG 3 cut(s) 57, 475, 498
Bse1I ACTGG 1 cut(s) 455
Bse3DI GCAATG 1 cut(s) 312
BseAI TCCGGA 1 cut(s) 640
BseDI CCNNGG 3 cut(s) 632, 674, 726
BseGI GGATG 2 cut(s) 154, 595
BseLI CCNNNNNNNGG 3 cut(s) 57, 475, 498
BseMI GCAATG 1 cut(s) 312
BseNI ACTGG 1 cut(s) 455
BseRI GAGGAG 2 cut(s) 691, 694
BseSI GKGCMC 2 cut(s) 184, 403
BseXI GCAGC 2 cut(s) 491, 600
Bsh1236I CGCG 1 cut(s) 669
BshFI GGCC 1 cut(s) 463
BshNI GGYRCC 1 cut(s) 688
BsiHKAI GWGCWC 2 cut(s) 184, 717
BsiHKCI CYCGRG 3 cut(s) 631, 707, 716
BsiSI CCGG 4 cut(s) 493, 599, 641, 653
BslFI GGGAC 1 cut(s) 707
BslI CCNNNNNNNGG 3 cut(s) 57, 475, 498
BsmAI GTCTC 1 cut(s) 216
BsmFI GGGAC 1 cut(s) 707
BsnI GGCC 1 cut(s) 463
BsoBI CYCGRG 3 cut(s) 631, 707, 716
Bsp1286I GDGCHC 3 cut(s) 184, 403, 717
Bsp13I TCCGGA 1 cut(s) 640
Bsp1407I TGTACA 1 cut(s) 259
Bsp143I GATC 3 cut(s) 81, 139, 592
BspACI CCGC 4 cut(s) 381, 610, 667, 685
BspANI GGCC 1 cut(s) 463
BspEI TCCGGA 1 cut(s) 640
BspFNI CGCG 1 cut(s) 669
BspLI GGNNCC 4 cut(s) 512, 690, 722, 723
BspPI GGATC 1 cut(s) 134
BspT107I GGYRCC 1 cut(s) 688
BsrDI GCAATG 1 cut(s) 312
BsrGI TGTACA 1 cut(s) 259
BsrI ACTGG 1 cut(s) 455
BssECI CCNNGG 3 cut(s) 632, 674, 726
BssMI GATC 3 cut(s) 81, 139, 592
BssSI CACGAG 1 cut(s) 402
BssT1I CCWWGG 1 cut(s) 726
Bst2BI CACGAG 1 cut(s) 402
Bst4CI ACNGT 1 cut(s) 167
BstAPI GCANNNNNTGC 1 cut(s) 465
BstAUI TGTACA 1 cut(s) 259
BstC8I GCNNGC 3 cut(s) 461, 650, 671
BstF5I GGATG 2 cut(s) 154, 595
BstFNI CGCG 1 cut(s) 669
BstHHI GCGC 1 cut(s) 337
BstKTI GATC 3 cut(s) 84, 142, 595
BstMAI GTCTC 1 cut(s) 216
BstMBI GATC 3 cut(s) 81, 139, 592
BstMWI GCNNNNNNNGC 2 cut(s) 465, 712
BstNSI RCATGY 1 cut(s) 610
BstSCI CCNGG 1 cut(s) 491
BstSLI GKGCMC 2 cut(s) 184, 403
BstUI CGCG 1 cut(s) 669
BstV1I GCAGC 2 cut(s) 491, 600
BstV2I GAAGAC 1 cut(s) 545
BstX2I RGATCY 1 cut(s) 139
BstYI RGATCY 1 cut(s) 139
BsuRI GGCC 1 cut(s) 463
BtgZI GCGATG 1 cut(s) 565
BtsCI GGATG 2 cut(s) 154, 595
BtsIMutI CAGTG 1 cut(s) 448
Cac8I GCNNGC 3 cut(s) 461, 650, 671
CfoI GCGC 1 cut(s) 337
Cfr13I GGNCC 3 cut(s) 424, 721, 730
Csp6I GTAC 2 cut(s) 152, 260
CviAII CATG 3 cut(s) 343, 498, 607
CviJI RGCY 8 cut(s) 371, 463, 482, 528, 565, 613, 673, 706
CviKI_1 RGCY 8 cut(s) 371, 463, 482, 528, 565, 613, 673, 706
CviQI GTAC 2 cut(s) 152, 260
DpnI GATC 3 cut(s) 83, 141, 594
DpnII GATC 3 cut(s) 81, 139, 592
DriI GACNNNNNGTC 1 cut(s) 430
Eam1105I GACNNNNNGTC 1 cut(s) 430
Eco130I CCWWGG 1 cut(s) 726
Eco147I AGGCCT 1 cut(s) 463
Eco47I GGWCC 3 cut(s) 424, 721, 730
Eco88I CYCGRG 3 cut(s) 631, 707, 716
EcoO109I RGGNCCY 2 cut(s) 424, 721
EcoT14I CCWWGG 1 cut(s) 726
ErhI CCWWGG 1 cut(s) 726
FaeI CATG 3 cut(s) 346, 501, 610
FaqI GGGAC 1 cut(s) 707
FatI CATG 3 cut(s) 342, 497, 606
FauI CCCGC 2 cut(s) 374, 678
FbaI TGATCA 1 cut(s) 592
FblI GTMKAC 1 cut(s) 627
Fnu4HI GCNGC 3 cut(s) 480, 611, 614
FokI GGATG 2 cut(s) 161, 582
Fsp4HI GCNGC 3 cut(s) 480, 611, 614
FspBI CTAG 3 cut(s) 74, 372, 736
FspI TGCGCA 1 cut(s) 336
GlaI GCGC 1 cut(s) 336
GluI GCNGC 3 cut(s) 480, 611, 614
GsuI CTGGAG 1 cut(s) 472
HaeIII GGCC 1 cut(s) 463
HapII CCGG 4 cut(s) 493, 599, 641, 653
HhaI GCGC 1 cut(s) 337
Hin1II CATG 3 cut(s) 346, 501, 610
Hin6I GCGC 1 cut(s) 335
HinP1I GCGC 1 cut(s) 335
HinfI GANTC 2 cut(s) 8, 98
HpaII CCGG 4 cut(s) 493, 599, 641, 653
HphI GGTGA 2 cut(s) 182, 613
Hpy166II GTNNAC 4 cut(s) 182, 322, 604, 628
Hpy188I TCNGA 1 cut(s) 148
Hpy188III TCNNGA 5 cut(s) 79, 213, 349, 569, 641
Hpy8I GTNNAC 4 cut(s) 182, 322, 604, 628
Hpy99I CGWCG 2 cut(s) 547, 623
HpyAV CCTTC 3 cut(s) 361, 437, 688
HpyCH4III ACNGT 1 cut(s) 167
HpyCH4V TGCA 4 cut(s) 93, 182, 355, 504
HpyF10VI GCNNNNNNNGC 2 cut(s) 465, 712
Hsp92II CATG 3 cut(s) 346, 501, 610
HspAI GCGC 1 cut(s) 335
KflI GGGWCCC 1 cut(s) 721
Kpn2I TCCGGA 1 cut(s) 640
Ksp22I TGATCA 1 cut(s) 592
Kzo9I GATC 3 cut(s) 81, 139, 592
LpnPI CCDG 9 cut(s) 135, 436, 445, 468, 506, 538, 612, 654, 666
Lsp1109I GCAGC 2 cut(s) 491, 600
LweI GCATC 3 cut(s) 291, 342, 513
MaeI CTAG 3 cut(s) 74, 372, 736
MaeIII GTNAC 1 cut(s) 586
MalI GATC 3 cut(s) 83, 141, 594
MboI GATC 3 cut(s) 81, 139, 592
MboII GAAGA 4 cut(s) 17, 80, 233, 550
MflI RGATCY 1 cut(s) 139
MhlI GDGCHC 3 cut(s) 184, 403, 717
MluCI AATT 6 cut(s) 15, 40, 199, 325, 391, 414
MlyI GAGTC 1 cut(s) 17
MmeI TCCRAC 1 cut(s) 226
MnlI CCTC 9 cut(s) 398, 415, 447, 540, 544, 627, 669, 672, 675
MroI TCCGGA 1 cut(s) 640
MseI TTAA 2 cut(s) 293, 390
MspA1I CMGCKG 1 cut(s) 482
MspI CCGG 4 cut(s) 493, 599, 641, 653
MspR9I CCNGG 1 cut(s) 493
MvnI CGCG 1 cut(s) 669
MwoI GCNNNNNNNGC 2 cut(s) 465, 712
NciI CCSGG 1 cut(s) 493
NdeII GATC 3 cut(s) 81, 139, 592
NlaIII CATG 3 cut(s) 346, 501, 610
NlaIV GGNNCC 4 cut(s) 512, 690, 722, 723
NmeAIII GCCGAG 1 cut(s) 699
NmuCI GTSAC 1 cut(s) 586
NsbI TGCGCA 1 cut(s) 336
NspI RCATGY 1 cut(s) 610
PceI AGGCCT 1 cut(s) 463
PcsI WCGNNNNNNNCGW 1 cut(s) 542
PfeI GAWTC 1 cut(s) 98
PflFI GACNNNGTC 1 cut(s) 623
PflMI CCANNNNNTGG 1 cut(s) 57
PkrI GCNGC 3 cut(s) 481, 612, 615
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
PpuMI RGGWCCY 2 cut(s) 424, 721
Psp5II RGGWCCY 2 cut(s) 424, 721
PspN4I GGNNCC 4 cut(s) 512, 690, 722, 723
PspPI GGNCC 3 cut(s) 424, 721, 730
PspPPI RGGWCCY 2 cut(s) 424, 721
PsuI RGATCY 1 cut(s) 139
PsyI GACNNNGTC 1 cut(s) 623
PvuII CAGCTG 1 cut(s) 482
RsaI GTAC 2 cut(s) 153, 261
RsaNI GTAC 2 cut(s) 152, 260
SaqAI TTAA 2 cut(s) 293, 390
SatI GCNGC 3 cut(s) 480, 611, 614
Sau3AI GATC 3 cut(s) 81, 139, 592
Sau96I GGNCC 3 cut(s) 424, 721, 730
SchI GAGTC 1 cut(s) 17
ScrFI CCNGG 1 cut(s) 493
SduI GDGCHC 3 cut(s) 184, 403, 717
SetI ASST 6 cut(s) 138, 409, 429, 484, 567, 638
SfaNI GCATC 3 cut(s) 291, 342, 513
SinI GGWCC 3 cut(s) 424, 721, 730
SpeI ACTAGT 1 cut(s) 73
Sse9I AATT 6 cut(s) 15, 40, 199, 325, 391, 414
SseBI AGGCCT 1 cut(s) 463
SsiI CCGC 4 cut(s) 381, 610, 667, 685
SspI AATATT 1 cut(s) 359
SspMI CTAG 3 cut(s) 74, 372, 736
StuI AGGCCT 1 cut(s) 463
StyD4I CCNGG 1 cut(s) 491
StyI CCWWGG 1 cut(s) 726
TaaI ACNGT 1 cut(s) 167
TaqI TCGA 5 cut(s) 214, 329, 420, 536, 618
TasI AATT 6 cut(s) 15, 40, 199, 325, 391, 414
TatI WGTACW 1 cut(s) 259
TauI GCSGC 1 cut(s) 613
TfiI GAWTC 1 cut(s) 98
Tru1I TTAA 2 cut(s) 293, 390
Tru9I TTAA 2 cut(s) 293, 390
TscAI CASTG 1 cut(s) 455
TseFI GTSAC 1 cut(s) 586
TseI GCWGC 2 cut(s) 479, 613
Tsp45I GTSAC 1 cut(s) 586
TspGWI ACGGA 1 cut(s) 420
TspRI CASTG 1 cut(s) 455
Tth111I GACNNNGTC 1 cut(s) 623
Van91I CCANNNNNTGG 1 cut(s) 57
VneI GTGCAC 1 cut(s) 180
VpaK11BI GGWCC 3 cut(s) 424, 721, 730
XapI RAATTY 1 cut(s) 15
XceI RCATGY 1 cut(s) 610
XmiI GTMKAC 1 cut(s) 627
XspI CTAG 3 cut(s) 74, 372, 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.