Rroxscaffold_6G00399640

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0031336
Physical Location & Seq
Reverse (-)
21631420 .. 21634145
2726 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00399640.1

Sequence Viewer

Length: 588 bp
ATGGCTGGGGCGAAAGAAGAGATATTGAAAGCCCGTACGGACAACAGGGAGTACAGGAGGATCGTCCTCCCTAACTCCCTTGAAGTCCTCCTCATCAGCGATCCTGATACTAACAAGTGTGCTGCTTCCATGAATGTCAGTGTTGGTTCTTTCAGCGACCCTGATGGCCTGGAGGGCCTTGCCCATTTCCTTGAGCATATGCTGTTTTATGCTAGTGAGAAGTACCCTTTGGAGGACAGCTACTTGAAGTACATCATCGAGCATGGAGGAAGAGGAAATGGTTTCACTTATGAAGAACACACCAGCTATCGCTTCGACATTAATCCCGATGGTTTTGAAGAGGCTCTGGACAGGTTTGCTCAGTTCTTTATTAAACCCTTGATGTCAGCTGATGCTACAGCGAGGAAAATTAAAGCTGTTAATTCAGCAAATCTGAAAACTCTGTTGTCCGATGGCTGGAGAATGAACCAGGTATTAATAATCAAGTGCTGGAGCCTACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCGAACAATTTATTTTTGAAATATTTCCAAGAACTATCATGATATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

195

Amino Acids

22.11

Weight (kDa)

5.26

Isoelectric Point (pI)

24.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 29 - 158 1.6e-31 Insulinase (Peptidase family M16)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031336)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0005123.1_g000007
rosa_roxburghii Rroxscaffold_6G00399640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 68, 95
AfaI GTAC 4 cut(s) 37, 53, 224, 251
AfiI CCNNNNNNNGG 2 cut(s) 232, 456
AgsI TTSAA 5 cut(s) 28, 83, 247, 338, 557
AjnI CCWGG 2 cut(s) 168, 468
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
AluBI AGCT 4 cut(s) 240, 306, 389, 416
AluI AGCT 4 cut(s) 240, 306, 389, 416
AlwI GGATC 2 cut(s) 68, 95
AoxI GGCC 2 cut(s) 166, 175
ApeKI GCWGC 1 cut(s) 122
AseI ATTAAT 2 cut(s) 321, 476
Asp700I GAANNNNTTC 1 cut(s) 561
AspS9I GGNCC 1 cut(s) 175
BbvI GCAGC 1 cut(s) 109
BccI CCATC 3 cut(s) 158, 323, 446
BciT130I CCWGG 2 cut(s) 170, 470
BfaI CTAG 1 cut(s) 213
BfmI CTRYAG 1 cut(s) 396
BglI GCCNNNNNGGC 1 cut(s) 174
BisI GCNGC 1 cut(s) 123
BlsI GCNGC 1 cut(s) 124
Bme1390I CCNGG 2 cut(s) 170, 470
BmgT120I GGNCC 1 cut(s) 175
BmiI GGNNCC 1 cut(s) 494
BmrFI CCNGG 2 cut(s) 170, 470
BmsI GCATC 1 cut(s) 382
BpmI CTGGAG 3 cut(s) 191, 478, 511
BpuEI CTTGAG 1 cut(s) 212
Bsc4I CCNNNNNNNGG 2 cut(s) 232, 456
BseBI CCWGG 2 cut(s) 170, 470
BseLI CCNNNNNNNGG 2 cut(s) 232, 456
BseMII CTCAG 1 cut(s) 374
BseRI GAGGAG 1 cut(s) 80
BseXI GCAGC 1 cut(s) 109
BseYI CCCAGC 1 cut(s) 5
BshFI GGCC 2 cut(s) 168, 177
BsiWI CGTACG 1 cut(s) 35
BslI CCNNNNNNNGG 2 cut(s) 232, 456
BsnI GGCC 2 cut(s) 168, 177
Bsp143I GATC 2 cut(s) 60, 100
BspANI GGCC 2 cut(s) 168, 177
BspCNI CTCAG 1 cut(s) 373
BspHI TCATGA 1 cut(s) 576
BspLI GGNNCC 1 cut(s) 494
BspPI GGATC 2 cut(s) 68, 95
BssMI GATC 2 cut(s) 60, 100
Bst2UI CCWGG 2 cut(s) 170, 470
Bst6I CTCTTC 3 cut(s) 12, 265, 333
BstDEI CTNAG 1 cut(s) 360
BstKTI GATC 2 cut(s) 63, 103
BstMBI GATC 2 cut(s) 60, 100
BstMWI GCNNNNNNNGC 1 cut(s) 174
BstNI CCWGG 2 cut(s) 170, 470
BstSCI CCNGG 2 cut(s) 168, 468
BstSFI CTRYAG 1 cut(s) 396
BstV1I GCAGC 1 cut(s) 109
BsuRI GGCC 2 cut(s) 168, 177
BtsIMutI CAGTG 1 cut(s) 145
CciI TCATGA 1 cut(s) 576
Cfr13I GGNCC 1 cut(s) 175
CsiI ACCWGGT 1 cut(s) 468
Csp6I GTAC 4 cut(s) 36, 52, 223, 250
CviAII CATG 3 cut(s) 130, 263, 577
CviQI GTAC 4 cut(s) 36, 52, 223, 250
DdeI CTNAG 1 cut(s) 360
DpnI GATC 2 cut(s) 62, 102
DpnII GATC 2 cut(s) 60, 100
Eam1104I CTCTTC 3 cut(s) 12, 265, 333
EarI CTCTTC 3 cut(s) 12, 265, 333
EcoO109I RGGNCCY 1 cut(s) 175
EcoRII CCWGG 2 cut(s) 168, 468
FaeI CATG 3 cut(s) 133, 266, 580
FaiI YATR 8 cut(s) 131, 198, 200, 210, 264, 291, 578, 586
FatI CATG 3 cut(s) 129, 262, 576
FauNDI CATATG 1 cut(s) 198
Fnu4HI GCNGC 1 cut(s) 123
Fsp4HI GCNGC 1 cut(s) 123
FspBI CTAG 1 cut(s) 213
GluI GCNGC 1 cut(s) 123
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 3 cut(s) 191, 478, 511
HaeIII GGCC 2 cut(s) 168, 177
Hin1II CATG 3 cut(s) 133, 266, 580
Hpy188I TCNGA 3 cut(s) 435, 451, 541
Hpy188III TCNNGA 4 cut(s) 104, 326, 347, 577
HpyF10VI GCNNNNNNNGC 1 cut(s) 174
HpyF3I CTNAG 1 cut(s) 360
Hsp92II CATG 3 cut(s) 133, 266, 580
Kzo9I GATC 2 cut(s) 60, 100
LmnI GCTCC 1 cut(s) 492
Lsp1109I GCAGC 1 cut(s) 109
LweI GCATC 1 cut(s) 382
MabI ACCWGGT 1 cut(s) 468
MaeI CTAG 1 cut(s) 213
MalI GATC 2 cut(s) 62, 102
MboI GATC 2 cut(s) 60, 100
MboII GAAGA 4 cut(s) 29, 282, 305, 350
MluCI AATT 3 cut(s) 408, 421, 545
MroXI GAANNNNTTC 1 cut(s) 561
MseI TTAA 5 cut(s) 321, 372, 411, 420, 476
MspA1I CMGCKG 1 cut(s) 389
MspR9I CCNGG 2 cut(s) 170, 470
MvaI CCWGG 2 cut(s) 170, 470
MwoI GCNNNNNNNGC 1 cut(s) 174
NdeI CATATG 1 cut(s) 198
NdeII GATC 2 cut(s) 60, 100
NlaIII CATG 3 cut(s) 133, 266, 580
NlaIV GGNNCC 1 cut(s) 494
PagI TCATGA 1 cut(s) 576
PdmI GAANNNNTTC 1 cut(s) 561
Pfl23II CGTACG 1 cut(s) 35
PkrI GCNGC 1 cut(s) 124
PshBI ATTAAT 2 cut(s) 321, 476
Psp6I CCWGG 2 cut(s) 168, 468
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 2 cut(s) 168, 468
PspLI CGTACG 1 cut(s) 35
PspN4I GGNNCC 1 cut(s) 494
PspPI GGNCC 1 cut(s) 175
PvuII CAGCTG 1 cut(s) 389
RsaI GTAC 4 cut(s) 37, 53, 224, 251
RsaNI GTAC 4 cut(s) 36, 52, 223, 250
SaqAI TTAA 5 cut(s) 321, 372, 411, 420, 476
SatI GCNGC 1 cut(s) 123
Sau3AI GATC 2 cut(s) 60, 100
Sau96I GGNCC 1 cut(s) 175
ScrFI CCNGG 2 cut(s) 170, 470
SetI ASST 6 cut(s) 242, 308, 356, 391, 418, 474
SexAI ACCWGGT 1 cut(s) 468
SfaNI GCATC 1 cut(s) 382
SfcI CTRYAG 1 cut(s) 396
SfiI GGCCNNNNNGGCC 1 cut(s) 174
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
Sse9I AATT 3 cut(s) 408, 421, 545
SspI AATATT 1 cut(s) 561
SspMI CTAG 1 cut(s) 213
StyD4I CCNGG 2 cut(s) 168, 468
TaqI TCGA 2 cut(s) 258, 315
TasI AATT 3 cut(s) 408, 421, 545
TatI WGTACW 2 cut(s) 51, 249
Tru1I TTAA 5 cut(s) 321, 372, 411, 420, 476
Tru9I TTAA 5 cut(s) 321, 372, 411, 420, 476
TscAI CASTG 1 cut(s) 145
TseI GCWGC 1 cut(s) 122
TspDTI ATGAA 3 cut(s) 146, 306, 479
TspGWI ACGGA 1 cut(s) 53
TspRI CASTG 1 cut(s) 145
VspI ATTAAT 2 cut(s) 321, 476
XmnI GAANNNNTTC 1 cut(s) 561
XspI CTAG 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.