Rroxscaffold_6G00404510

disease resistance RPP13-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0025867
Physical Location & Seq
Forward (+)
27010015 .. 27012567
2553 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00404510.1

Sequence Viewer

Length: 1080 bp
ATGTCCAGAAATACCCTTGTCACTACTTCATTTAAGACACTTTATGTGTCTCTGGATCTTCTTTCGTACTTGAAATCTAAGCAGGTCACACCATTCCAGATTTTCAATGACGTTATCATGCCAGATTTTGAAGACCACACATCCAACGGAAACCACAGGACCAATAGTGATGAAATGAAAATGATACTTCAAGAAATCCAAAATGATCTCATTTCCATCAGTGGGGCCTGTGGGGGATTGCAGAAATGGGAAGATGATGTTAAATTCAAGTTAAATTCTGAAACCTTACAACTTTCAAATACCCCTACGCTGGTGAGGAACTACGAGGAGTTCACAGATACTTTGAGCAAAGAAGGCAAAACTGCTAAGGAGACCGGGAATGAATTCTTTAAATTGTTTATGGAGAAAAGCCTTATCCAGCCTGTCTACGAAAAGCGCAGGCCAAGTGCAGACAGCTGCAGCATGCAGCCTAAAACTCGTGAAGCTGTGATCAAGCTTGCAAAGAAAACCAGATTCTTCACAAATGGAAATTTAACGAAAGATTTCTCTTCCTCTAAGAGGACATGCCTGGTAAGAATTGTAGAAGGATCTAATATACGCGAATTGCCTTTTCCATTCAACTCGGGAGATGGCAAAGCTCAGCAAATTAAGCAGATCACTGAAGTAGAGGACAGTGAATTCCTAAAAGGCATGAAGAATATGAGTCATCTCTGGTCTGGAGGCCGTGACTGTAAGCTAGATGACTTGACAAAATTGGAAAATCCAAGGAAGCTGAGTATCCACATAGACAGAGAGCAAGCTGAAACAGCTGAAAGAGAGCTGAATTCTTTGGCGAAGTTCAATAACCTCCGGTCACTATCCATATCATGGTCACAAATATATGGCAAGACTGCAACTCGAACTCTCCAGAGGACATTCACCGAGAAGGTGTTGAGGACACTCACCAAGAAGCTGAACAGAATGAAAAAGGTAACAAAACCGCTTTCAATAGCTCCGTTAACGGTTCCATCAGTTACTCCAGATTCTCCATTCACTGTTGTTTTGGAGAAACTTGACCTCTATTACTTCCCTCAAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

359

Amino Acids

41.11

Weight (kDa)

9.25

Isoelectric Point (pI)

40.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025867)

Species Orthologous Gene IDs
rosa_roxburghii Rroxscaffold_6G00404510 Rroxscaffold_6G00404950
rosa_rugosa Rorug03G0157300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 73
AccB7I CCANNNNNTGG 1 cut(s) 867
AccI GTMKAC 1 cut(s) 426
AccII CGCG 1 cut(s) 600
AciI CCGC 1 cut(s) 980
AclWI GGATC 2 cut(s) 63, 595
AcsI RAATTY 6 cut(s) 263, 274, 383, 529, 677, 823
AcuI CTGAAG 1 cut(s) 681
AfaI GTAC 1 cut(s) 68
AfiI CCNNNNNNNGG 4 cut(s) 222, 310, 558, 867
AgsI TTSAA 9 cut(s) 73, 106, 131, 191, 268, 297, 619, 841, 987
AjnI CCWGG 1 cut(s) 567
Alw26I GTCTC 2 cut(s) 54, 365
AlwI GGATC 2 cut(s) 63, 595
Ama87I CYCGRG 1 cut(s) 622
AoxI GGCC 3 cut(s) 225, 440, 721
ApeKI GCWGC 3 cut(s) 456, 459, 466
ApoI RAATTY 6 cut(s) 263, 274, 383, 529, 677, 823
Asp700I GAANNNNTTC 2 cut(s) 383, 542
AspLEI GCGC 1 cut(s) 438
AspS9I GGNCC 2 cut(s) 159, 225
AsuC2I CCSGG 1 cut(s) 376
AsuHPI GGTGA 3 cut(s) 325, 910, 934
AvaI CYCGRG 1 cut(s) 622
AvaII GGWCC 1 cut(s) 159
BauI CACGAG 1 cut(s) 477
BbsI GAAGAC 1 cut(s) 138
BbvI GCAGC 3 cut(s) 443, 471, 478
BccI CCATC 3 cut(s) 224, 623, 1015
BceAI ACGGC 1 cut(s) 708
BciT130I CCWGG 1 cut(s) 569
BciVI GTATCC 1 cut(s) 788
BclI TGATCA 1 cut(s) 489
BcnI CCSGG 1 cut(s) 376
BcoDI GTCTC 2 cut(s) 54, 365
BfaI CTAG 1 cut(s) 737
BfmI CTRYAG 1 cut(s) 457
BfuAI ACCTGC 1 cut(s) 73
BfuI GTATCC 1 cut(s) 788
BisI GCNGC 3 cut(s) 457, 460, 467
BlpI GCTNAGC 1 cut(s) 639
BlsI GCNGC 3 cut(s) 458, 461, 468
Bme1390I CCNGG 2 cut(s) 376, 569
Bme18I GGWCC 1 cut(s) 159
BmeT110I CYCGRG 1 cut(s) 622
BmgT120I GGNCC 2 cut(s) 159, 225
BmiI GGNNCC 2 cut(s) 226, 1005
BmrFI CCNGG 2 cut(s) 376, 569
BpiI GAAGAC 1 cut(s) 138
BpmI CTGGAG 3 cut(s) 738, 890, 1002
Bpu10I CCTNAGC 1 cut(s) 366
Bpu1102I GCTNAGC 1 cut(s) 639
BpuMI CCSGG 1 cut(s) 376
BsaI GGTCTC 1 cut(s) 365
BsaJI CCNNGG 1 cut(s) 764
BsaWI WCCGGW 1 cut(s) 849
Bsc4I CCNNNNNNNGG 4 cut(s) 222, 310, 558, 867
BseBI CCWGG 1 cut(s) 569
BseDI CCNNGG 1 cut(s) 764
BseGI GGATG 1 cut(s) 140
BseLI CCNNNNNNNGG 4 cut(s) 222, 310, 558, 867
BseMII CTCAG 2 cut(s) 653, 764
BseRI GAGGAG 1 cut(s) 341
BseXI GCAGC 3 cut(s) 443, 471, 478
BsgI GTGCAG 1 cut(s) 468
Bsh1236I CGCG 1 cut(s) 600
BshFI GGCC 3 cut(s) 227, 442, 723
BsiHKCI CYCGRG 1 cut(s) 622
BsiSI CCGG 2 cut(s) 375, 850
BslI CCNNNNNNNGG 4 cut(s) 222, 310, 558, 867
BsmAI GTCTC 2 cut(s) 54, 365
BsnI GGCC 3 cut(s) 227, 442, 723
Bso31I GGTCTC 1 cut(s) 365
BsoBI CYCGRG 1 cut(s) 622
Bsp143I GATC 5 cut(s) 55, 205, 489, 587, 654
Bsp1720I GCTNAGC 1 cut(s) 639
BspACI CCGC 1 cut(s) 980
BspANI GGCC 3 cut(s) 227, 442, 723
BspCNI CTCAG 2 cut(s) 652, 765
BspFNI CGCG 1 cut(s) 600
BspLI GGNNCC 2 cut(s) 226, 1005
BspMAI CTGCAG 1 cut(s) 461
BspMI ACCTGC 1 cut(s) 73
BspPI GGATC 2 cut(s) 63, 595
BspTNI GGTCTC 1 cut(s) 365
BssECI CCNNGG 1 cut(s) 764
BssMI GATC 5 cut(s) 55, 205, 489, 587, 654
BssSI CACGAG 1 cut(s) 477
BssT1I CCWWGG 1 cut(s) 764
Bst2BI CACGAG 1 cut(s) 477
Bst2UI CCWGG 1 cut(s) 569
Bst4CI ACNGT 4 cut(s) 674, 731, 1003, 1036
Bst6I CTCTTC 1 cut(s) 553
BstC8I GCNNGC 4 cut(s) 440, 464, 498, 798
BstDEI CTNAG 5 cut(s) 78, 366, 555, 639, 773
BstENI CCTNNNNNAGG 1 cut(s) 556
BstF5I GGATG 1 cut(s) 140
BstFNI CGCG 1 cut(s) 600
BstHHI GCGC 1 cut(s) 438
BstKTI GATC 5 cut(s) 58, 208, 492, 590, 657
BstMAI GTCTC 2 cut(s) 54, 365
BstMBI GATC 5 cut(s) 55, 205, 489, 587, 654
BstMWI GCNNNNNNNGC 3 cut(s) 354, 649, 806
BstNI CCWGG 1 cut(s) 569
BstNSI RCATGY 2 cut(s) 466, 567
BstSCI CCNGG 2 cut(s) 374, 567
BstSFI CTRYAG 1 cut(s) 457
BstUI CGCG 1 cut(s) 600
BstV1I GCAGC 3 cut(s) 443, 471, 478
BstV2I GAAGAC 1 cut(s) 138
BstX2I RGATCY 2 cut(s) 55, 587
BstYI RGATCY 2 cut(s) 55, 587
BsuI GTATCC 1 cut(s) 788
BsuRI GGCC 3 cut(s) 227, 442, 723
BtsCI GGATG 1 cut(s) 140
BtsIMutI CAGTG 4 cut(s) 226, 657, 679, 1032
BveI ACCTGC 1 cut(s) 73
Cac8I GCNNGC 4 cut(s) 440, 464, 498, 798
CfoI GCGC 1 cut(s) 438
Cfr13I GGNCC 2 cut(s) 159, 225
Csp6I GTAC 1 cut(s) 67
CviAII CATG 5 cut(s) 118, 463, 564, 691, 867
CviQI GTAC 1 cut(s) 67
DdeI CTNAG 5 cut(s) 78, 366, 555, 639, 773
DpnI GATC 5 cut(s) 57, 207, 491, 589, 656
DpnII GATC 5 cut(s) 55, 205, 489, 587, 654
DraI TTTAAA 1 cut(s) 391
Eam1104I CTCTTC 1 cut(s) 553
EarI CTCTTC 1 cut(s) 553
Eco130I CCWWGG 1 cut(s) 764
Eco31I GGTCTC 1 cut(s) 365
Eco47I GGWCC 1 cut(s) 159
Eco57I CTGAAG 1 cut(s) 681
Eco88I CYCGRG 1 cut(s) 622
EcoNI CCTNNNNNAGG 1 cut(s) 556
EcoO109I RGGNCCY 1 cut(s) 225
EcoRI GAATTC 3 cut(s) 383, 677, 823
EcoRII CCWGG 1 cut(s) 567
EcoT14I CCWWGG 1 cut(s) 764
ErhI CCWWGG 1 cut(s) 764
FaeI CATG 5 cut(s) 121, 466, 567, 694, 870
FatI CATG 5 cut(s) 117, 462, 563, 690, 866
FbaI TGATCA 1 cut(s) 489
FblI GTMKAC 1 cut(s) 426
Fnu4HI GCNGC 3 cut(s) 457, 460, 467
FokI GGATG 1 cut(s) 127
Fsp4HI GCNGC 3 cut(s) 457, 460, 467
FspBI CTAG 1 cut(s) 737
GlaI GCGC 1 cut(s) 437
GluI GCNGC 3 cut(s) 457, 460, 467
GsuI CTGGAG 3 cut(s) 738, 890, 1002
HaeIII GGCC 3 cut(s) 227, 442, 723
HapII CCGG 2 cut(s) 375, 850
HhaI GCGC 1 cut(s) 438
Hin1II CATG 5 cut(s) 121, 466, 567, 694, 870
Hin6I GCGC 1 cut(s) 436
HinP1I GCGC 1 cut(s) 436
HincII GTYRAC 1 cut(s) 999
HindII GTYRAC 1 cut(s) 999
HindIII AAGCTT 1 cut(s) 494
HinfI GANTC 3 cut(s) 513, 703, 1022
HpaI GTTAAC 1 cut(s) 999
HpaII CCGG 2 cut(s) 375, 850
HphI GGTGA 3 cut(s) 325, 910, 934
Hpy166II GTNNAC 3 cut(s) 333, 427, 999
Hpy188I TCNGA 1 cut(s) 280
Hpy188III TCNNGA 9 cut(s) 6, 53, 97, 191, 479, 624, 717, 907, 1019
Hpy8I GTNNAC 3 cut(s) 333, 427, 999
HpyAV CCTTC 3 cut(s) 347, 578, 919
HpyCH4III ACNGT 4 cut(s) 674, 731, 1003, 1036
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 6 cut(s) 241, 449, 459, 466, 500, 893
HpyF10VI GCNNNNNNNGC 3 cut(s) 354, 649, 806
HpyF3I CTNAG 5 cut(s) 78, 366, 555, 639, 773
HpySE526I ACGT 1 cut(s) 111
Hsp92II CATG 5 cut(s) 121, 466, 567, 694, 870
HspAI GCGC 1 cut(s) 436
Ksp22I TGATCA 1 cut(s) 489
KspAI GTTAAC 1 cut(s) 999
Kzo9I GATC 5 cut(s) 55, 205, 489, 587, 654
LmnI GCTCC 1 cut(s) 997
Lsp1109I GCAGC 3 cut(s) 443, 471, 478
MaeI CTAG 1 cut(s) 737
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 7 cut(s) 19, 85, 725, 852, 870, 970, 1012
MalI GATC 5 cut(s) 57, 207, 491, 589, 656
MboI GATC 5 cut(s) 55, 205, 489, 587, 654
MboII GAAGA 6 cut(s) 50, 143, 263, 508, 540, 706
MflI RGATCY 2 cut(s) 55, 587
MlyI GAGTC 1 cut(s) 712
MmeI TCCRAC 1 cut(s) 168
MroXI GAANNNNTTC 2 cut(s) 383, 542
MseI TTAA 7 cut(s) 33, 261, 272, 390, 533, 648, 998
MspA1I CMGCKG 2 cut(s) 456, 809
MspI CCGG 2 cut(s) 375, 850
MspR9I CCNGG 2 cut(s) 376, 569
MvaI CCWGG 1 cut(s) 569
MvnI CGCG 1 cut(s) 600
MwoI GCNNNNNNNGC 3 cut(s) 354, 649, 806
NciI CCSGG 1 cut(s) 376
NdeII GATC 5 cut(s) 55, 205, 489, 587, 654
NlaIII CATG 5 cut(s) 121, 466, 567, 694, 870
NlaIV GGNNCC 2 cut(s) 226, 1005
NmuCI GTSAC 5 cut(s) 19, 85, 725, 852, 870
NspI RCATGY 2 cut(s) 466, 567
PaeI GCATGC 1 cut(s) 466
PdmI GAANNNNTTC 2 cut(s) 383, 542
PfeI GAWTC 2 cut(s) 513, 1022
PflMI CCANNNNNTGG 1 cut(s) 867
PkrI GCNGC 3 cut(s) 458, 461, 468
PleI GAGTC 1 cut(s) 711
PpsI GAGTC 1 cut(s) 711
Psp6I CCWGG 1 cut(s) 567
PspGI CCWGG 1 cut(s) 567
PspN4I GGNNCC 2 cut(s) 226, 1005
PspPI GGNCC 2 cut(s) 159, 225
PstI CTGCAG 1 cut(s) 461
PsuI RGATCY 2 cut(s) 55, 587
PvuII CAGCTG 2 cut(s) 456, 809
RsaI GTAC 1 cut(s) 68
RsaNI GTAC 1 cut(s) 67
SaqAI TTAA 7 cut(s) 33, 261, 272, 390, 533, 648, 998
SatI GCNGC 3 cut(s) 457, 460, 467
Sau3AI GATC 5 cut(s) 55, 205, 489, 587, 654
Sau96I GGNCC 2 cut(s) 159, 225
SchI GAGTC 1 cut(s) 712
ScrFI CCNGG 2 cut(s) 376, 569
SfcI CTRYAG 1 cut(s) 457
SinI GGWCC 1 cut(s) 159
SphI GCATGC 1 cut(s) 466
SsiI CCGC 1 cut(s) 980
SspMI CTAG 1 cut(s) 737
StyD4I CCNGG 2 cut(s) 374, 567
StyI CCWWGG 1 cut(s) 764
TaaI ACNGT 4 cut(s) 674, 731, 1003, 1036
TaiI ACGT 1 cut(s) 114
TaqI TCGA 1 cut(s) 898
TfiI GAWTC 2 cut(s) 513, 1022
Tru1I TTAA 7 cut(s) 33, 261, 272, 390, 533, 648, 998
Tru9I TTAA 7 cut(s) 33, 261, 272, 390, 533, 648, 998
TscAI CASTG 4 cut(s) 226, 664, 679, 1039
TseFI GTSAC 5 cut(s) 19, 85, 725, 852, 870
TseI GCWGC 3 cut(s) 456, 459, 466
Tsp45I GTSAC 5 cut(s) 19, 85, 725, 852, 870
TspDTI ATGAA 6 cut(s) 18, 186, 191, 396, 707, 977
TspGWI ACGGA 2 cut(s) 162, 984
TspRI CASTG 4 cut(s) 226, 664, 679, 1039
Van91I CCANNNNNTGG 1 cut(s) 867
VpaK11BI GGWCC 1 cut(s) 159
XagI CCTNNNNNAGG 1 cut(s) 556
XapI RAATTY 6 cut(s) 263, 274, 383, 529, 677, 823
XceI RCATGY 2 cut(s) 466, 567
XmiI GTMKAC 1 cut(s) 426
XmnI GAANNNNTTC 2 cut(s) 383, 542
XspI CTAG 1 cut(s) 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.