Rorug03G0157300

disease resistance RPP13-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0025867
Physical Location & Seq
Forward (+)
12951580 .. 12952482
903 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0157300.1

Sequence Viewer

Length: 903 bp
ATGGATATGGAGATACTTAGTTGGAATTGCCGAGGGATCTGCAATGACACGACAAAGAGGGCGCTGAAGGATTTAATTTCCCAGAATCATCCTCAAATCATTTTTCTTTGTGAGACGAAGATCAGCACTCTTGCTGACTTCCGTGAGTTGCATCGGGCTGTTGGATTTCCGCATTCAAATGAGGTGTTGAGTGATGGTTTATCAGGAGGTTTGGCGATGTTCTGGACTGATGATGTACAAGCGACAGTTTTGACTTTTTCTGCGCATCACATCGACTTGGAGATTGCAGGAGGTCCTGGAGATCCACGTTGGAGGTTAACGGAATTCTATGGATATGCTAGGACGACAGAAAGAGATCGATCATGGCAACTGTTACAGAATTTGCGTGATCTCGATTCTTTACCCTGGGTTGTTATTGGGGATTTCAATGAAATCCTGAATAATGGAGAAAAGATTGCGGGGACGCTTCGTTCGGAACGTCAGATGTGTGGATTTCGTGAAGCTCTTGGATATGGTGACCTTCTTGATTTAGGGTTTCAGGGTGCTATGTCTACATGGTGGAATTCTGACACACAACTCTGGTTAGATCGTGCAGTTTGTACACCCTGTTGGTGGGATATCTACGGGCATGCACGAGTGTGTCATCTAGCTCCTAGTGATTCTGACCATGTTCCGATACTCTTACAAGCCAGCTCTGTTCCCTTGGCCAAAAGACCAAGAGTGCACCGGTTCAAATTCGAAGCTTACTGGCTTCAACATAATGATTGTGATGAGGTTGTGAAGCAAGCATGGGCGACAGACGTGACTGGAAGTCCTATGTACTGTGCAGCCAAAAAGATTGAGTTCACAAGGATACAGCTTAATGATTGGCAGAAGAGAGCTTTCAGGACTAGGCAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

300

Amino Acids

34.49

Weight (kDa)

5.93

Isoelectric Point (pI)

43.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 6 - 220 5.1e-18 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025867)

Species Orthologous Gene IDs
rosa_roxburghii Rroxscaffold_6G00404510 Rroxscaffold_6G00404950
rosa_rugosa Rorug03G0157300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 264
AccI GTMKAC 1 cut(s) 551
AciI CCGC 2 cut(s) 170, 458
AclWI GGATC 2 cut(s) 44, 296
AcoI YGGCCR 1 cut(s) 705
AcsI RAATTY 4 cut(s) 323, 379, 562, 734
AcuI CTGAAG 1 cut(s) 86
AfaI GTAC 3 cut(s) 237, 601, 821
AfiI CCNNNNNNNGG 1 cut(s) 612
AgeI ACCGGT 1 cut(s) 726
AgsI TTSAA 4 cut(s) 177, 427, 733, 755
AhdI GACNNNNNGTC 1 cut(s) 810
AjiI CACGTC 1 cut(s) 802
AjnI CCWGG 2 cut(s) 295, 404
AleI CACNNNNGTG 1 cut(s) 637
AloI GAACNNNNNNTCC 2 cut(s) 454, 486
AluBI AGCT 6 cut(s) 503, 650, 693, 743, 859, 881
AluI AGCT 6 cut(s) 503, 650, 693, 743, 859, 881
Alw21I GWGCWC 1 cut(s) 726
Alw26I GTCTC 1 cut(s) 107
Alw44I GTGCAC 1 cut(s) 722
AlwI GGATC 2 cut(s) 44, 296
AoxI GGCC 1 cut(s) 705
ApaLI GTGCAC 1 cut(s) 722
ApeKI GCWGC 2 cut(s) 827, 895
ApoI RAATTY 4 cut(s) 323, 379, 562, 734
ArsI GACNNNNNNTTYG 2 cut(s) 454, 486
AsiGI ACCGGT 1 cut(s) 726
AspLEI GCGC 2 cut(s) 64, 265
AspS9I GGNCC 1 cut(s) 293
AsuHPI GGTGA 1 cut(s) 527
AsuII TTCGAA 1 cut(s) 738
AvaII GGWCC 1 cut(s) 293
BaeGI GKGCMC 1 cut(s) 726
BalI TGGCCA 1 cut(s) 707
BauI CACGAG 1 cut(s) 633
Bbv12I GWGCWC 1 cut(s) 726
BbvI GCAGC 1 cut(s) 839
BccI CCATC 1 cut(s) 188
BcgI CGANNNNNNTGC 2 cut(s) 21, 55
BciT130I CCWGG 2 cut(s) 297, 406
BciVI GTATCC 1 cut(s) 846
BcoDI GTCTC 1 cut(s) 107
BfaI CTAG 4 cut(s) 339, 647, 654, 891
BfoI RGCGCY 1 cut(s) 65
BfuI GTATCC 1 cut(s) 846
BisI GCNGC 2 cut(s) 828, 896
BlsI GCNGC 2 cut(s) 829, 897
Bme1390I CCNGG 2 cut(s) 297, 406
Bme18I GGWCC 1 cut(s) 293
BmeRI GACNNNNNGTC 1 cut(s) 810
BmgBI CACGTC 1 cut(s) 802
BmgT120I GGNCC 1 cut(s) 293
BmrFI CCNGG 2 cut(s) 297, 406
BmsI GCATC 2 cut(s) 160, 274
BpmI CTGGAG 1 cut(s) 318
Bpu14I TTCGAA 1 cut(s) 738
Bsa29I ATCGAT 1 cut(s) 358
BsaJI CCNNGG 4 cut(s) 31, 404, 405, 702
BsaWI WCCGGW 1 cut(s) 726
Bsc4I CCNNNNNNNGG 1 cut(s) 612
Bse118I RCCGGY 1 cut(s) 726
Bse1I ACTGG 2 cut(s) 752, 811
Bse3DI GCAATG 1 cut(s) 49
BseBI CCWGG 2 cut(s) 297, 406
BseCI ATCGAT 1 cut(s) 358
BseDI CCNNGG 4 cut(s) 31, 404, 405, 702
BseGI GGATG 1 cut(s) 88
BseLI CCNNNNNNNGG 1 cut(s) 612
BseMI GCAATG 1 cut(s) 49
BseNI ACTGG 2 cut(s) 752, 811
BseSI GKGCMC 1 cut(s) 726
BseXI GCAGC 1 cut(s) 839
BsgI GTGCAG 2 cut(s) 612, 846
BshFI GGCC 1 cut(s) 707
BshTI ACCGGT 1 cut(s) 726
BshVI ATCGAT 1 cut(s) 358
BsiHKAI GWGCWC 1 cut(s) 726
BsiSI CCGG 1 cut(s) 727
BslFI GGGAC 1 cut(s) 475
BslI CCNNNNNNNGG 1 cut(s) 612
BsmAI GTCTC 1 cut(s) 107
BsmBI CGTCTC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 475
BsmI GAATGC 1 cut(s) 172
BsnI GGCC 1 cut(s) 707
Bsp119I TTCGAA 1 cut(s) 738
Bsp1286I GDGCHC 1 cut(s) 726
Bsp1407I TGTACA 2 cut(s) 235, 599
Bsp143I GATC 7 cut(s) 36, 120, 301, 355, 359, 388, 586
BspACI CCGC 2 cut(s) 170, 458
BspANI GGCC 1 cut(s) 707
BspDI ATCGAT 1 cut(s) 358
BspPI GGATC 2 cut(s) 44, 296
BspT104I TTCGAA 1 cut(s) 738
BsrDI GCAATG 1 cut(s) 49
BsrFI RCCGGY 1 cut(s) 726
BsrGI TGTACA 2 cut(s) 235, 599
BsrI ACTGG 2 cut(s) 752, 811
BssAI RCCGGY 1 cut(s) 726
BssECI CCNNGG 4 cut(s) 31, 404, 405, 702
BssMI GATC 7 cut(s) 36, 120, 301, 355, 359, 388, 586
BssSI CACGAG 1 cut(s) 633
BssT1I CCWWGG 1 cut(s) 702
Bst2BI CACGAG 1 cut(s) 633
Bst2UI CCWGG 2 cut(s) 297, 406
Bst4CI ACNGT 3 cut(s) 247, 372, 824
Bst6I CTCTTC 1 cut(s) 869
BstAUI TGTACA 2 cut(s) 235, 599
BstBI TTCGAA 1 cut(s) 738
BstC8I GCNNGC 3 cut(s) 630, 691, 786
BstDEI CTNAG 1 cut(s) 17
BstEII GGTNACC 1 cut(s) 515
BstF5I GGATG 1 cut(s) 88
BstH2I RGCGCY 1 cut(s) 65
BstHHI GCGC 2 cut(s) 64, 265
BstKTI GATC 7 cut(s) 39, 123, 304, 358, 362, 391, 589
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 7 cut(s) 36, 120, 301, 355, 359, 388, 586
BstNI CCWGG 2 cut(s) 297, 406
BstNSI RCATGY 1 cut(s) 632
BstPI GGTNACC 1 cut(s) 515
BstSCI CCNGG 2 cut(s) 295, 404
BstSLI GKGCMC 1 cut(s) 726
BstV1I GCAGC 1 cut(s) 839
BstX2I RGATCY 2 cut(s) 36, 301
BstYI RGATCY 2 cut(s) 36, 301
Bsu15I ATCGAT 1 cut(s) 358
BsuI GTATCC 1 cut(s) 846
BsuRI GGCC 1 cut(s) 707
BsuTUI ATCGAT 1 cut(s) 358
BtgZI GCGATG 1 cut(s) 230
BtrI CACGTC 1 cut(s) 802
BtsCI GGATG 1 cut(s) 88
Cac8I GCNNGC 3 cut(s) 630, 691, 786
CfoI GCGC 2 cut(s) 64, 265
Cfr10I RCCGGY 1 cut(s) 726
Cfr13I GGNCC 1 cut(s) 293
ClaI ATCGAT 1 cut(s) 358
CseI GACGC 1 cut(s) 472
Csp6I GTAC 3 cut(s) 236, 600, 820
CspAI ACCGGT 1 cut(s) 726
CviAII CATG 5 cut(s) 363, 555, 629, 668, 789
CviQI GTAC 3 cut(s) 236, 600, 820
DdeI CTNAG 1 cut(s) 17
DpnI GATC 7 cut(s) 38, 122, 303, 357, 361, 390, 588
DpnII GATC 7 cut(s) 36, 120, 301, 355, 359, 388, 586
DriI GACNNNNNGTC 1 cut(s) 810
EaeI YGGCCR 1 cut(s) 705
Eam1104I CTCTTC 1 cut(s) 869
Eam1105I GACNNNNNGTC 1 cut(s) 810
EarI CTCTTC 1 cut(s) 869
Eco130I CCWWGG 1 cut(s) 702
Eco32I GATATC 1 cut(s) 619
Eco47I GGWCC 1 cut(s) 293
Eco57I CTGAAG 1 cut(s) 86
Eco91I GGTNACC 1 cut(s) 515
EcoO109I RGGNCCY 1 cut(s) 293
EcoO65I GGTNACC 1 cut(s) 515
EcoRI GAATTC 2 cut(s) 323, 562
EcoRII CCWGG 2 cut(s) 295, 404
EcoRV GATATC 1 cut(s) 619
EcoT14I CCWWGG 1 cut(s) 702
ErhI CCWWGG 1 cut(s) 702
Esp3I CGTCTC 1 cut(s) 107
FaeI CATG 5 cut(s) 366, 558, 632, 671, 792
FaqI GGGAC 1 cut(s) 475
FatI CATG 5 cut(s) 362, 554, 628, 667, 788
FauI CCCGC 1 cut(s) 451
FblI GTMKAC 1 cut(s) 551
Fnu4HI GCNGC 2 cut(s) 828, 896
FokI GGATG 1 cut(s) 75
Fsp4HI GCNGC 2 cut(s) 828, 896
FspBI CTAG 4 cut(s) 339, 647, 654, 891
FspI TGCGCA 1 cut(s) 264
GlaI GCGC 2 cut(s) 63, 264
GluI GCNGC 2 cut(s) 828, 896
GsuI CTGGAG 1 cut(s) 318
HaeII RGCGCY 1 cut(s) 65
HaeIII GGCC 1 cut(s) 707
HapII CCGG 1 cut(s) 727
HgaI GACGC 1 cut(s) 472
HhaI GCGC 2 cut(s) 64, 265
Hin1II CATG 5 cut(s) 366, 558, 632, 671, 792
Hin6I GCGC 2 cut(s) 62, 263
HinP1I GCGC 2 cut(s) 62, 263
HincII GTYRAC 1 cut(s) 318
HindII GTYRAC 1 cut(s) 318
HindIII AAGCTT 1 cut(s) 741
HinfI GANTC 3 cut(s) 85, 395, 659
HpaI GTTAAC 1 cut(s) 318
HpaII CCGG 1 cut(s) 727
HphI GGTGA 1 cut(s) 527
Hpy166II GTNNAC 5 cut(s) 318, 552, 602, 724, 846
Hpy188I TCNGA 5 cut(s) 475, 483, 568, 664, 675
Hpy188III TCNNGA 7 cut(s) 204, 223, 392, 436, 497, 524, 886
Hpy8I GTNNAC 5 cut(s) 318, 552, 602, 724, 846
HpyAV CCTTC 2 cut(s) 61, 530
HpyCH4III ACNGT 3 cut(s) 247, 372, 824
HpyCH4IV ACGT 3 cut(s) 307, 478, 801
HpyCH4V TGCA 7 cut(s) 42, 151, 287, 593, 632, 724, 827
HpyF3I CTNAG 1 cut(s) 17
HpySE526I ACGT 3 cut(s) 307, 478, 801
Hsp92II CATG 5 cut(s) 366, 558, 632, 671, 792
HspAI GCGC 2 cut(s) 62, 263
KspAI GTTAAC 1 cut(s) 318
Kzo9I GATC 7 cut(s) 36, 120, 301, 355, 359, 388, 586
LmnI GCTCC 1 cut(s) 655
Lsp1109I GCAGC 1 cut(s) 839
LweI GCATC 2 cut(s) 160, 274
MaeI CTAG 4 cut(s) 339, 647, 654, 891
MaeII ACGT 3 cut(s) 307, 478, 801
MaeIII GTNAC 3 cut(s) 372, 515, 802
MalI GATC 7 cut(s) 38, 122, 303, 357, 361, 390, 588
MboI GATC 7 cut(s) 36, 120, 301, 355, 359, 388, 586
MboII GAAGA 2 cut(s) 130, 886
MflI RGATCY 2 cut(s) 36, 301
MhlI GDGCHC 1 cut(s) 726
MlsI TGGCCA 1 cut(s) 707
MluCI AATT 6 cut(s) 25, 75, 323, 379, 562, 734
MluNI TGGCCA 1 cut(s) 707
MmeI TCCRAC 2 cut(s) 142, 290
MnlI CCTC 8 cut(s) 26, 51, 102, 175, 200, 284, 306, 766
Mox20I TGGCCA 1 cut(s) 707
MscI TGGCCA 1 cut(s) 707
MseI TTAA 3 cut(s) 74, 317, 861
MslI CAYNNNNRTG 2 cut(s) 177, 637
Msp20I TGGCCA 1 cut(s) 707
MspI CCGG 1 cut(s) 727
MspR9I CCNGG 2 cut(s) 297, 406
Mva1269I GAATGC 1 cut(s) 172
MvaI CCWGG 2 cut(s) 297, 406
NdeII GATC 7 cut(s) 36, 120, 301, 355, 359, 388, 586
NlaIII CATG 5 cut(s) 366, 558, 632, 671, 792
NmeAIII GCCGAG 1 cut(s) 56
NmuCI GTSAC 2 cut(s) 515, 802
NsbI TGCGCA 1 cut(s) 264
NspI RCATGY 1 cut(s) 632
NspV TTCGAA 1 cut(s) 738
OliI CACNNNNGTG 1 cut(s) 637
PaeI GCATGC 1 cut(s) 632
PasI CCCWGGG 1 cut(s) 405
PcsI WCGNNNNNNNCGW 1 cut(s) 475
PctI GAATGC 1 cut(s) 172
PfeI GAWTC 3 cut(s) 85, 395, 659
PfoI TCCNGGA 1 cut(s) 295
PinAI ACCGGT 1 cut(s) 726
PkrI GCNGC 2 cut(s) 829, 897
PpuMI RGGWCCY 1 cut(s) 293
Psp5II RGGWCCY 1 cut(s) 293
Psp6I CCWGG 2 cut(s) 295, 404
PspEI GGTNACC 1 cut(s) 515
PspGI CCWGG 2 cut(s) 295, 404
PspPI GGNCC 1 cut(s) 293
PspPPI RGGWCCY 1 cut(s) 293
PsuI RGATCY 2 cut(s) 36, 301
RsaI GTAC 3 cut(s) 237, 601, 821
RsaNI GTAC 3 cut(s) 236, 600, 820
RseI CAYNNNNRTG 2 cut(s) 177, 637
SaqAI TTAA 3 cut(s) 74, 317, 861
SatI GCNGC 2 cut(s) 828, 896
Sau3AI GATC 7 cut(s) 36, 120, 301, 355, 359, 388, 586
Sau96I GGNCC 1 cut(s) 293
ScrFI CCNGG 2 cut(s) 297, 406
SduI GDGCHC 1 cut(s) 726
SfaNI GCATC 2 cut(s) 160, 274
SfuI TTCGAA 1 cut(s) 738
SinI GGWCC 1 cut(s) 293
SmiMI CAYNNNNRTG 2 cut(s) 177, 637
SphI GCATGC 1 cut(s) 632
Sse9I AATT 6 cut(s) 25, 75, 323, 379, 562, 734
SsiI CCGC 2 cut(s) 170, 458
SspMI CTAG 4 cut(s) 339, 647, 654, 891
StyD4I CCNGG 2 cut(s) 295, 404
StyI CCWWGG 1 cut(s) 702
TaaI ACNGT 3 cut(s) 247, 372, 824
TaiI ACGT 3 cut(s) 310, 481, 804
TaqI TCGA 4 cut(s) 273, 358, 393, 738
TasI AATT 6 cut(s) 25, 75, 323, 379, 562, 734
TatI WGTACW 3 cut(s) 235, 599, 819
TfiI GAWTC 3 cut(s) 85, 395, 659
Tru1I TTAA 3 cut(s) 74, 317, 861
Tru9I TTAA 3 cut(s) 74, 317, 861
TseFI GTSAC 2 cut(s) 515, 802
TseI GCWGC 2 cut(s) 827, 895
Tsp45I GTSAC 2 cut(s) 515, 802
TspDTI ATGAA 1 cut(s) 444
TspGWI ACGGA 2 cut(s) 131, 335
VneI GTGCAC 1 cut(s) 722
VpaK11BI GGWCC 1 cut(s) 293
XapI RAATTY 4 cut(s) 323, 379, 562, 734
XceI RCATGY 1 cut(s) 632
XmiI GTMKAC 1 cut(s) 551
XspI CTAG 4 cut(s) 339, 647, 654, 891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.