Rroxscaffold_7G00175590

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0031652
Physical Location & Seq
Reverse (-)
15109985 .. 15110712
728 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00175590.1

Sequence Viewer

Length: 639 bp
ATGCTCAGGAAGAGTCCCGAACATAGACCAACAGCTGCAGAGTTATTAAGGCATCCACACTTACAACCATACCTCCTTCAATGCCGCAATGCATCATCTGTTTTTCTTCCAGTATTTCCTATAAACAACTTGAAAGATAAAACTACAAGGAAATCACCATCTAGCAAGCCCATTGGTGGCAAGGACAGCCGAGACAAAGAAGCTGCGACACCTAAGCACTTGGAAAAGGTTCATCCATTCGAGACCAGACCTGATGCTATGCAGAGATATTCAATAAACATTGCCAACCCTGTATTCACAGCTAGTACTGAAGAAAAGCTTGAAACCAAGAGAGTTGATCCTAGTAGCTGTACTGTGGAACCATCTAATGCCACTGCTGCAACGCAAAGAACTGAAAGAAGACCAAATCTGCATAATTTAATAATCCATTACAGTTCACAATCTGACACTTCATTGAAAATGTGCACACAACTAAATGAATTGAACAAAGTTCAAACATCTATCACAAGTCTTATGTGTTTGAAGCAAGACTCGAAACCACGGCGAAGCCGACGACGGAGAAACCACTTCCCCGAGCCGACGACCGGACCAGGAAGGCAAGAACTTTGGGAAATTGGGAATGGGTCGGTGTCTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

212

Amino Acids

24.09

Weight (kDa)

10.01

Isoelectric Point (pI)

77.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031652)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8171598.1_g000001
rosa_roxburghii Rroxscaffold_7G00175590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 85
AclWI GGATC 1 cut(s) 332
AcuI CTGAAG 1 cut(s) 330
AfaI GTAC 2 cut(s) 307, 352
AfiI CCNNNNNNNGG 2 cut(s) 176, 584
AgsI TTSAA 8 cut(s) 80, 133, 273, 323, 457, 484, 494, 523
AjnI CCWGG 1 cut(s) 589
AjuI GAANNNNNNNTTGG 2 cut(s) 278, 310
AluBI AGCT 5 cut(s) 35, 203, 302, 319, 348
AluI AGCT 5 cut(s) 35, 203, 302, 319, 348
Alw21I GWGCWC 1 cut(s) 467
Alw26I GTCTC 2 cut(s) 186, 236
Alw44I GTGCAC 1 cut(s) 463
AlwI GGATC 1 cut(s) 332
Ama87I CYCGRG 1 cut(s) 572
ApaLI GTGCAC 1 cut(s) 463
ApeKI GCWGC 3 cut(s) 35, 203, 377
Asp700I GAANNNNTTC 1 cut(s) 228
AspS9I GGNCC 1 cut(s) 587
AsuHPI GGTGA 1 cut(s) 147
AvaI CYCGRG 1 cut(s) 572
AvaII GGWCC 1 cut(s) 587
BaeGI GKGCMC 1 cut(s) 467
BbsI GAAGAC 1 cut(s) 406
Bbv12I GWGCWC 1 cut(s) 467
BbvI GCAGC 3 cut(s) 22, 190, 364
BccI CCATC 2 cut(s) 166, 370
BceAI ACGGC 1 cut(s) 557
BciT130I CCWGG 1 cut(s) 591
BcoDI GTCTC 2 cut(s) 186, 236
BfaI CTAG 3 cut(s) 162, 303, 342
BfmI CTRYAG 1 cut(s) 36
BisI GCNGC 4 cut(s) 36, 85, 204, 378
BlsI GCNGC 4 cut(s) 37, 86, 205, 379
BmcAI AGTACT 1 cut(s) 307
Bme1390I CCNGG 1 cut(s) 591
Bme18I GGWCC 1 cut(s) 587
BmeT110I CYCGRG 1 cut(s) 572
BmgT120I GGNCC 1 cut(s) 587
BmiI GGNNCC 1 cut(s) 360
BmrFI CCNGG 1 cut(s) 591
BmsI GCATC 3 cut(s) 61, 101, 244
BpiI GAAGAC 1 cut(s) 406
Bpu10I CCTNAGC 2 cut(s) 5, 213
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 1 cut(s) 539
BsaWI WCCGGW 1 cut(s) 584
BsaXI ACNNNNNCTCC 2 cut(s) 57, 87
Bsc4I CCNNNNNNNGG 2 cut(s) 176, 584
Bse1I ACTGG 1 cut(s) 110
Bse3DI GCAATG 2 cut(s) 94, 279
BseBI CCWGG 1 cut(s) 591
BseDI CCNNGG 1 cut(s) 539
BseGI GGATG 2 cut(s) 52, 232
BseLI CCNNNNNNNGG 2 cut(s) 176, 584
BseMI GCAATG 2 cut(s) 94, 279
BseMII CTCAG 1 cut(s) 19
BseNI ACTGG 1 cut(s) 110
BseSI GKGCMC 1 cut(s) 467
BseXI GCAGC 3 cut(s) 22, 190, 364
Bsh1285I CGRYCG 1 cut(s) 585
BsiEI CGRYCG 1 cut(s) 585
BsiHKAI GWGCWC 1 cut(s) 467
BsiHKCI CYCGRG 1 cut(s) 572
BsiSI CCGG 1 cut(s) 585
BslI CCNNNNNNNGG 2 cut(s) 176, 584
BsmAI GTCTC 2 cut(s) 186, 236
Bso31I GGTCTC 1 cut(s) 236
BsoBI CYCGRG 1 cut(s) 572
Bsp1286I GDGCHC 1 cut(s) 467
Bsp143I GATC 1 cut(s) 337
BspACI CCGC 1 cut(s) 85
BspCNI CTCAG 1 cut(s) 18
BspLI GGNNCC 1 cut(s) 360
BspMAI CTGCAG 1 cut(s) 40
BspPI GGATC 1 cut(s) 332
BspTNI GGTCTC 1 cut(s) 236
BsrDI GCAATG 2 cut(s) 94, 279
BsrI ACTGG 1 cut(s) 110
BssECI CCNNGG 1 cut(s) 539
BssMI GATC 1 cut(s) 337
Bst2UI CCWGG 1 cut(s) 591
Bst4CI ACNGT 2 cut(s) 355, 434
Bst6I CTCTTC 1 cut(s) 5
BstC8I GCNNGC 1 cut(s) 167
BstDEI CTNAG 2 cut(s) 5, 213
BstDSI CCRYGG 1 cut(s) 539
BstF5I GGATG 2 cut(s) 52, 232
BstKTI GATC 1 cut(s) 340
BstMAI GTCTC 2 cut(s) 186, 236
BstMBI GATC 1 cut(s) 337
BstMCI CGRYCG 1 cut(s) 585
BstMWI GCNNNNNNNGC 2 cut(s) 186, 377
BstNI CCWGG 1 cut(s) 591
BstSCI CCNGG 1 cut(s) 589
BstSFI CTRYAG 1 cut(s) 36
BstSLI GKGCMC 1 cut(s) 467
BstV1I GCAGC 3 cut(s) 22, 190, 364
BstV2I GAAGAC 1 cut(s) 406
BtgI CCRYGG 1 cut(s) 539
BtsCI GGATG 2 cut(s) 52, 232
BtsI GCAGTG 1 cut(s) 372
BtsIMutI CAGTG 1 cut(s) 372
Cac8I GCNNGC 1 cut(s) 167
Cfr13I GGNCC 1 cut(s) 587
Csp6I GTAC 2 cut(s) 306, 351
CviJI RGCY 9 cut(s) 35, 169, 189, 203, 302, 319, 348, 549, 577
CviKI_1 RGCY 9 cut(s) 35, 169, 189, 203, 302, 319, 348, 549, 577
CviQI GTAC 2 cut(s) 306, 351
DdeI CTNAG 2 cut(s) 5, 213
DpnI GATC 1 cut(s) 339
DpnII GATC 1 cut(s) 337
Eam1104I CTCTTC 1 cut(s) 5
EarI CTCTTC 1 cut(s) 5
Eco31I GGTCTC 1 cut(s) 236
Eco47I GGWCC 1 cut(s) 587
Eco57I CTGAAG 1 cut(s) 330
Eco88I CYCGRG 1 cut(s) 572
EcoRII CCWGG 1 cut(s) 589
EcoT22I ATGCAT 1 cut(s) 94
FaiI YATR 6 cut(s) 24, 70, 122, 260, 414, 515
FalI AAGNNNNNCTT 2 cut(s) 303, 335
Fnu4HI GCNGC 4 cut(s) 36, 85, 204, 378
FokI GGATG 2 cut(s) 39, 219
Fsp4HI GCNGC 4 cut(s) 36, 85, 204, 378
FspBI CTAG 3 cut(s) 162, 303, 342
GluI GCNGC 4 cut(s) 36, 85, 204, 378
HapII CCGG 1 cut(s) 585
HindIII AAGCTT 1 cut(s) 317
HinfI GANTC 2 cut(s) 13, 530
HpaII CCGG 1 cut(s) 585
HphI GGTGA 1 cut(s) 147
Hpy166II GTNNAC 2 cut(s) 437, 465
Hpy188I TCNGA 1 cut(s) 445
Hpy188III TCNNGA 3 cut(s) 7, 17, 241
Hpy8I GTNNAC 2 cut(s) 437, 465
Hpy99I CGWCG 3 cut(s) 555, 558, 583
HpyAV CCTTC 2 cut(s) 86, 588
HpyCH4III ACNGT 2 cut(s) 355, 434
HpyCH4V TGCA 6 cut(s) 38, 92, 262, 380, 412, 465
HpyF10VI GCNNNNNNNGC 2 cut(s) 186, 377
HpyF3I CTNAG 2 cut(s) 5, 213
Kzo9I GATC 1 cut(s) 337
LpnPI CCDG 7 cut(s) 123, 259, 264, 303, 576, 598, 603
Lsp1109I GCAGC 3 cut(s) 22, 190, 364
LweI GCATC 3 cut(s) 61, 101, 244
MaeI CTAG 3 cut(s) 162, 303, 342
MalI GATC 1 cut(s) 339
MboI GATC 1 cut(s) 337
MboII GAAGA 4 cut(s) 22, 98, 323, 411
MhlI GDGCHC 1 cut(s) 467
MluCI AATT 3 cut(s) 415, 479, 612
MlyI GAGTC 2 cut(s) 22, 524
MnlI CCTC 1 cut(s) 83
Mph1103I ATGCAT 1 cut(s) 94
MroXI GAANNNNTTC 1 cut(s) 228
MseI TTAA 2 cut(s) 47, 419
MspA1I CMGCKG 1 cut(s) 35
MspI CCGG 1 cut(s) 585
MspR9I CCNGG 1 cut(s) 591
MvaI CCWGG 1 cut(s) 591
MwoI GCNNNNNNNGC 2 cut(s) 186, 377
NdeII GATC 1 cut(s) 337
NlaIV GGNNCC 1 cut(s) 360
NmeAIII GCCGAG 1 cut(s) 215
NsiI ATGCAT 1 cut(s) 94
PcsI WCGNNNNNNNCGW 1 cut(s) 547
PdmI GAANNNNTTC 1 cut(s) 228
PkrI GCNGC 4 cut(s) 37, 86, 205, 379
PleI GAGTC 2 cut(s) 21, 524
PpsI GAGTC 2 cut(s) 21, 524
Psp6I CCWGG 1 cut(s) 589
PspGI CCWGG 1 cut(s) 589
PspN4I GGNNCC 1 cut(s) 360
PspPI GGNCC 1 cut(s) 587
PstI CTGCAG 1 cut(s) 40
PvuII CAGCTG 1 cut(s) 35
RsaI GTAC 2 cut(s) 307, 352
RsaNI GTAC 2 cut(s) 306, 351
SaqAI TTAA 2 cut(s) 47, 419
SatI GCNGC 4 cut(s) 36, 85, 204, 378
Sau3AI GATC 1 cut(s) 337
Sau96I GGNCC 1 cut(s) 587
ScaI AGTACT 1 cut(s) 307
SchI GAGTC 2 cut(s) 22, 524
ScrFI CCNGG 1 cut(s) 591
SduI GDGCHC 1 cut(s) 467
SetI ASST 9 cut(s) 37, 75, 205, 214, 231, 253, 304, 321, 350
SfaNI GCATC 3 cut(s) 61, 101, 244
SfcI CTRYAG 1 cut(s) 36
SinI GGWCC 1 cut(s) 587
Sse9I AATT 3 cut(s) 415, 479, 612
SsiI CCGC 1 cut(s) 85
SspMI CTAG 3 cut(s) 162, 303, 342
StyD4I CCNGG 1 cut(s) 589
TaaI ACNGT 2 cut(s) 355, 434
TaqI TCGA 2 cut(s) 240, 533
TasI AATT 3 cut(s) 415, 479, 612
TatI WGTACW 2 cut(s) 305, 350
TauI GCSGC 1 cut(s) 87
Tru1I TTAA 2 cut(s) 47, 419
Tru9I TTAA 2 cut(s) 47, 419
TscAI CASTG 1 cut(s) 379
TseI GCWGC 3 cut(s) 35, 203, 377
TspDTI ATGAA 3 cut(s) 221, 441, 492
TspGWI ACGGA 1 cut(s) 571
TspRI CASTG 1 cut(s) 379
VneI GTGCAC 1 cut(s) 463
VpaK11BI GGWCC 1 cut(s) 587
XmnI GAANNNNTTC 1 cut(s) 228
XspI CTAG 3 cut(s) 162, 303, 342
ZrmI AGTACT 1 cut(s) 307
Zsp2I ATGCAT 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.