Rorug01G0021900

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0032251
Physical Location & Seq
Reverse (-)
3570136 .. 3573728
3593 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0021900.1

Sequence Viewer

Length: 990 bp
ATGAGCGGAGCAGGGAAAGGTGTTGTCTGTGTAACAGGAGCATCTGGTTATATTGCATCATGGCTGGTCAAGCTTTTGCTACAACGTGGTTATATTGTCAGAGGCACTGTTCGGGACCCAAACGATCCCAAAAAAACAGAACACTTACTTTCACTTGAGGGGGCAAAAGAAAGGCTTCATTTGTTCAAAGCAGATTTGCTAGATGAAGGATCTTTTGACCCTGTAGTTGATGGATGTGACTGTGTTTTTCATACCGCATCTCCGGTATCTTTTTCATCAGTCAGTGACCCTCAGGCAGAACTAATTGACCCTGCTGTGAAGGGAACATTAAATGTTCTGAAATCATGTGCAAAATTTCCAACCGTCAAGCGAGTGGTTTTGACATCTTCTTTGGCTTCAGTAATGATGAATGGAAAACCTCTGACCCCTGATGTTGTAGTGGATGAAACATGGTATTCAGATCCACTAATTGCTGAGAAGCACAAGCTGTGGTATATTCTTTCAAAAACTTTAGCTGAGGAGGCTGCTTGGAAATTTGCTGAAGGAAATGGGATTGACTTGGTTTCAATAAATCCGGGGTTTGTGATTGGACCACTCTTACAGCCGATAATTAATTTGACGGTGGAGATGATTCAGAACCTCATAACTGGCAAGGCAGTGCCCGCTTCAAATTACAGATTTATTGATGTTAGAGATGTTGCCGCTGCACATATTCAAGCATTTGAAATTCTTTCAGCTAGTGGAAGGTATTGTTTGGTCAGCCATGTTGCAAATATTTCCGAGACTCTAGAGATGATCCGACAACTTTATCCCACCATGTACATTCCTGAAAGATGTGAGGATGACAATCCTCTTTTGAATCCTTCTGGCCCACAGTTTCAAGTATCCAAGGAGAAAGCAAAAAGTCTGGGAATTAGTTTTCAGCCATTGGAAGTAACTCTGAAGGACACTATTGAAAGCCTGAAGGAAAAGGGTTTCCTCAACAGATGA

Protein Analysis

329

Amino Acids

36.02

Weight (kDa)

5.75

Isoelectric Point (pI)

24.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 106 5.3e-09 NmrA-like family
Epimerase PF01370 9 - 242 1.8e-25 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 246 6.4e-19 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 130 1.1e-12 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 11 - 201 5.8e-12 Male sterility protein
NAD_binding_10 PF13460 13 - 133 1.5e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0032251)

Species Orthologous Gene IDs
rosa_rugosa Rorug01G0021900
rosa_samantha Rh1BG117600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 6
AciI CCGC 4 cut(s) 6, 255, 663, 702
AclWI GGATC 4 cut(s) 119, 217, 455, 790
AcsI RAATTY 3 cut(s) 353, 533, 726
AcuI CTGAAG 4 cut(s) 381, 561, 962, 983
AfaI GTAC 1 cut(s) 821
AgsI TTSAA 9 cut(s) 187, 504, 567, 669, 716, 725, 859, 881, 956
AluBI AGCT 4 cut(s) 73, 487, 515, 737
AluI AGCT 4 cut(s) 73, 487, 515, 737
Alw26I GTCTC 1 cut(s) 776
AlwI GGATC 4 cut(s) 119, 217, 455, 790
AoxI GGCC 1 cut(s) 868
ApeKI GCWGC 2 cut(s) 524, 704
ApoI RAATTY 3 cut(s) 353, 533, 726
ArsI GACNNNNNNTTYG 2 cut(s) 373, 405
AseI ATTAAT 1 cut(s) 612
Asp700I GAANNNNTTC 1 cut(s) 174
AspS9I GGNCC 3 cut(s) 115, 590, 869
AsuC2I CCSGG 1 cut(s) 576
AvaII GGWCC 2 cut(s) 115, 590
AxyI CCTNAGG 1 cut(s) 291
BaeGI GKGCMC 1 cut(s) 663
BbvCI CCTCAGC 1 cut(s) 516
BbvI GCAGC 2 cut(s) 511, 691
BccI CCATC 1 cut(s) 224
BciVI GTATCC 1 cut(s) 895
BcnI CCSGG 1 cut(s) 576
BcoDI GTCTC 1 cut(s) 776
BfaI CTAG 3 cut(s) 200, 738, 788
BfmI CTRYAG 1 cut(s) 222
BfuI GTATCC 1 cut(s) 895
BisI GCNGC 3 cut(s) 525, 702, 705
BlsI GCNGC 3 cut(s) 526, 703, 706
Bme1390I CCNGG 1 cut(s) 576
Bme18I GGWCC 2 cut(s) 115, 590
BmgT120I GGNCC 3 cut(s) 115, 590, 869
BmiI GGNNCC 2 cut(s) 116, 117
BmrFI CCNGG 1 cut(s) 576
BmsI GCATC 3 cut(s) 50, 65, 266
Bpu10I CCTNAGC 1 cut(s) 516
BpuEI CTTGAG 1 cut(s) 176
BpuMI CCSGG 1 cut(s) 576
BsaBI GATNNNNATC 1 cut(s) 846
BsaJI CCNNGG 2 cut(s) 575, 888
BsaWI WCCGGW 1 cut(s) 262
BsaXI ACNNNNNCTCC 2 cut(s) 244, 274
Bse1I ACTGG 1 cut(s) 652
Bse21I CCTNAGG 1 cut(s) 291
Bse8I GATNNNNATC 1 cut(s) 846
BseDI CCNNGG 2 cut(s) 575, 888
BseGI GGATG 3 cut(s) 239, 448, 847
BseJI GATNNNNATC 1 cut(s) 846
BseMII CTCAG 3 cut(s) 305, 465, 507
BseNI ACTGG 1 cut(s) 652
BseRI GAGGAG 1 cut(s) 533
BseSI GKGCMC 1 cut(s) 663
BseXI GCAGC 2 cut(s) 511, 691
BsgI GTGCAG 1 cut(s) 690
BshFI GGCC 1 cut(s) 870
BsiSI CCGG 2 cut(s) 263, 575
BslFI GGGAC 1 cut(s) 128
BsmAI GTCTC 1 cut(s) 776
BsmFI GGGAC 1 cut(s) 128
BsnI GGCC 1 cut(s) 870
Bsp1286I GDGCHC 1 cut(s) 663
Bsp1407I TGTACA 1 cut(s) 819
Bsp143I GATC 4 cut(s) 124, 209, 460, 795
BspACI CCGC 4 cut(s) 6, 255, 663, 702
BspANI GGCC 1 cut(s) 870
BspCNI CTCAG 3 cut(s) 304, 466, 508
BspLI GGNNCC 2 cut(s) 116, 117
BspPI GGATC 4 cut(s) 119, 217, 455, 790
BsrBI CCGCTC 1 cut(s) 6
BsrGI TGTACA 1 cut(s) 819
BsrI ACTGG 1 cut(s) 652
BssECI CCNNGG 2 cut(s) 575, 888
BssMI GATC 4 cut(s) 124, 209, 460, 795
BssT1I CCWWGG 1 cut(s) 888
Bst4CI ACNGT 5 cut(s) 109, 242, 364, 622, 876
BstAUI TGTACA 1 cut(s) 819
BstC8I GCNNGC 1 cut(s) 663
BstDEI CTNAG 3 cut(s) 291, 474, 516
BstF5I GGATG 3 cut(s) 239, 448, 847
BstKTI GATC 4 cut(s) 127, 212, 463, 798
BstMAI GTCTC 1 cut(s) 776
BstMBI GATC 4 cut(s) 124, 209, 460, 795
BstMWI GCNNNNNNNGC 3 cut(s) 70, 521, 662
BstSCI CCNGG 1 cut(s) 574
BstSFI CTRYAG 1 cut(s) 222
BstSLI GKGCMC 1 cut(s) 663
BstV1I GCAGC 2 cut(s) 511, 691
BstX2I RGATCY 2 cut(s) 209, 460
BstYI RGATCY 2 cut(s) 209, 460
Bsu36I CCTNAGG 1 cut(s) 291
BsuI GTATCC 1 cut(s) 895
BsuRI GGCC 1 cut(s) 870
BtsCI GGATG 3 cut(s) 239, 448, 847
BtsI GCAGTG 1 cut(s) 663
BtsIMutI CAGTG 3 cut(s) 105, 289, 663
Cac8I GCNNGC 1 cut(s) 663
Cfr13I GGNCC 3 cut(s) 115, 590, 869
Csp6I GTAC 1 cut(s) 820
CviAII CATG 5 cut(s) 60, 345, 450, 764, 817
CviQI GTAC 1 cut(s) 820
DdeI CTNAG 3 cut(s) 291, 474, 516
DpnI GATC 4 cut(s) 126, 211, 462, 797
DpnII GATC 4 cut(s) 124, 209, 460, 795
Eco130I CCWWGG 1 cut(s) 888
Eco47I GGWCC 2 cut(s) 115, 590
Eco57I CTGAAG 4 cut(s) 381, 561, 962, 983
Eco81I CCTNAGG 1 cut(s) 291
EcoO109I RGGNCCY 1 cut(s) 115
EcoT14I CCWWGG 1 cut(s) 888
ErhI CCWWGG 1 cut(s) 888
FaeI CATG 5 cut(s) 63, 348, 453, 767, 820
FalI AAGNNNNNCTT 2 cut(s) 159, 191
FaqI GGGAC 1 cut(s) 128
FatI CATG 5 cut(s) 59, 344, 449, 763, 816
FauI CCCGC 1 cut(s) 670
Fnu4HI GCNGC 3 cut(s) 525, 702, 705
FokI GGATG 3 cut(s) 246, 455, 854
Fsp4HI GCNGC 3 cut(s) 525, 702, 705
FspBI CTAG 3 cut(s) 200, 738, 788
GluI GCNGC 3 cut(s) 525, 702, 705
HaeIII GGCC 1 cut(s) 870
HapII CCGG 2 cut(s) 263, 575
Hin1II CATG 5 cut(s) 63, 348, 453, 767, 820
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 3 cut(s) 631, 784, 859
HpaII CCGG 2 cut(s) 263, 575
Hpy188I TCNGA 8 cut(s) 101, 339, 423, 460, 636, 781, 800, 942
Hpy188III TCNNGA 3 cut(s) 113, 788, 827
HpyAV CCTTC 7 cut(s) 200, 313, 536, 738, 873, 937, 958
HpyCH4III ACNGT 5 cut(s) 109, 242, 364, 622, 876
HpyCH4IV ACGT 1 cut(s) 85
HpyCH4V TGCA 4 cut(s) 56, 350, 707, 770
HpyF10VI GCNNNNNNNGC 3 cut(s) 70, 521, 662
HpyF3I CTNAG 3 cut(s) 291, 474, 516
HpySE526I ACGT 1 cut(s) 85
Hsp92II CATG 5 cut(s) 63, 348, 453, 767, 820
KflI GGGWCCC 1 cut(s) 115
Kzo9I GATC 4 cut(s) 124, 209, 460, 795
LmnI GCTCC 2 cut(s) 8, 38
Lsp1109I GCAGC 2 cut(s) 511, 691
LweI GCATC 3 cut(s) 50, 65, 266
MaeI CTAG 3 cut(s) 200, 738, 788
MaeII ACGT 1 cut(s) 85
MaeIII GTNAC 4 cut(s) 31, 236, 284, 934
MalI GATC 4 cut(s) 126, 211, 462, 797
MbiI CCGCTC 1 cut(s) 6
MboI GATC 4 cut(s) 124, 209, 460, 795
MboII GAAGA 1 cut(s) 378
MflI RGATCY 2 cut(s) 209, 460
MhlI GDGCHC 1 cut(s) 663
MluCI AATT 9 cut(s) 303, 353, 468, 533, 609, 613, 670, 726, 912
MlyI GAGTC 1 cut(s) 778
MmeI TCCRAC 2 cut(s) 383, 823
MroXI GAANNNNTTC 1 cut(s) 174
MseI TTAA 2 cut(s) 329, 612
MspA1I CMGCKG 1 cut(s) 704
MspI CCGG 2 cut(s) 263, 575
MspR9I CCNGG 1 cut(s) 576
MwoI GCNNNNNNNGC 3 cut(s) 70, 521, 662
NciI CCSGG 1 cut(s) 576
NdeII GATC 4 cut(s) 124, 209, 460, 795
NlaIII CATG 5 cut(s) 63, 348, 453, 767, 820
NlaIV GGNNCC 2 cut(s) 116, 117
NmuCI GTSAC 2 cut(s) 236, 284
PdmI GAANNNNTTC 1 cut(s) 174
PfeI GAWTC 2 cut(s) 631, 859
PkrI GCNGC 3 cut(s) 526, 703, 706
PleI GAGTC 1 cut(s) 778
PpsI GAGTC 1 cut(s) 778
PpuMI RGGWCCY 1 cut(s) 115
PshBI ATTAAT 1 cut(s) 612
Psp5II RGGWCCY 1 cut(s) 115
PspN4I GGNNCC 2 cut(s) 116, 117
PspPI GGNCC 3 cut(s) 115, 590, 869
PspPPI RGGWCCY 1 cut(s) 115
PsuI RGATCY 2 cut(s) 209, 460
RsaI GTAC 1 cut(s) 821
RsaNI GTAC 1 cut(s) 820
SaqAI TTAA 2 cut(s) 329, 612
SatI GCNGC 3 cut(s) 525, 702, 705
Sau3AI GATC 4 cut(s) 124, 209, 460, 795
Sau96I GGNCC 3 cut(s) 115, 590, 869
SchI GAGTC 1 cut(s) 778
ScrFI CCNGG 1 cut(s) 576
SduI GDGCHC 1 cut(s) 663
SetI ASST 9 cut(s) 22, 75, 88, 421, 489, 517, 642, 739, 749
SfaNI GCATC 3 cut(s) 50, 65, 266
SfcI CTRYAG 1 cut(s) 222
SinI GGWCC 2 cut(s) 115, 590
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
Sse9I AATT 9 cut(s) 303, 353, 468, 533, 609, 613, 670, 726, 912
SsiI CCGC 4 cut(s) 6, 255, 663, 702
SspI AATATT 1 cut(s) 775
SspMI CTAG 3 cut(s) 200, 738, 788
StyD4I CCNGG 1 cut(s) 574
StyI CCWWGG 1 cut(s) 888
TaaI ACNGT 5 cut(s) 109, 242, 364, 622, 876
TaiI ACGT 1 cut(s) 88
TasI AATT 9 cut(s) 303, 353, 468, 533, 609, 613, 670, 726, 912
TatI WGTACW 1 cut(s) 819
TauI GCSGC 1 cut(s) 704
TfiI GAWTC 2 cut(s) 631, 859
Tru1I TTAA 2 cut(s) 329, 612
Tru9I TTAA 2 cut(s) 329, 612
TscAI CASTG 3 cut(s) 112, 289, 663
TseFI GTSAC 2 cut(s) 236, 284
TseI GCWGC 2 cut(s) 524, 704
Tsp45I GTSAC 2 cut(s) 236, 284
TspDTI ATGAA 6 cut(s) 167, 219, 239, 264, 422, 459
TspRI CASTG 3 cut(s) 112, 289, 663
VpaK11BI GGWCC 2 cut(s) 115, 590
VspI ATTAAT 1 cut(s) 612
XapI RAATTY 3 cut(s) 353, 533, 726
XbaI TCTAGA 1 cut(s) 787
XmnI GAANNNNTTC 1 cut(s) 174
XspI CTAG 3 cut(s) 200, 738, 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.