Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0025517

Physical Location & Seq

Genomic Coordinates Forward (+)

15220371 .. 15224465

Gene Structure 4095 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0096900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4095 bp

ATGGCGCTGCGAGTACTGGCTTGGAATTGTCGAGGAATAGTTAGTAATTCCAGGAGAAGAGCACTGATTGATCTAATCAGACAAGAAAAACCAGCAGTTCTTTTTCTTTCTGAAACCCTATGTGACAAACACCAACTCGAAACTCTACGAATCAAAGTAGGATTCGATAATTGCCTAGGATGGCCCAAAGGAGATGATAGCCAAGGCATAGCGTTCCTTTGGATGAATGATGCGAAAGTACGCATCCGAAATTATTCTGCAAGGCATGTGGATGTAGCCATTGGAGAGAGTGGATCACTAGACGAATGGAGGCTAACAGGGATTTATGGTTATGCACAAACAGCAGATCGACACCAGACATGGGATCTAATGAGACAACTTGCAGCCCAATCAAACCTACCATGGTTGGTAGTTGGCGATTTCAACGAGATAGCTGACAATCAGGAAAAGACAGGAGGAATACCTAGAAGACATGGACAGATTCAAGCTTTTCGACAGGCCATGATGGACTGCTCACTTATAGACATGGGGTATTATGGTAGTCCATATACGTGGTCGGATTACCAAACCAAAGAAAGATTGGATAGAAGCTTATGGAACGTAGAGCTACGAGGATTATTCCCCTCGTCTCAGACCACCCACCTGCCCCCAAGCTCTTCAGACCACAGTCCTCTGCTAGTGGATATTAGTGAAGCCCCACTCCCCCGATCTGGTAAACGCAAATCTCCCTTCAGATTCGAACAGTTTTGGGCAACTCACAACGAATGTGAAGTAGTGATCAAACGAGGATGGCAACAGATAACAGATGGAGACCCGATGCAGCAGGTATGCCAACGTATTAAACAGACGGGAAAGGAACTCACAAGTTGGAACAAAGAAACATTTATGTTCCGACAAGTGGAGATGAGGGCAGTGAGAGCTCGTCTCGATGAATTACTAAGCAAGCCTTATGACGCAAATGATCATGGAGAGAAACAGCAGCTGCAGGCAAAATTTCAAGAATTGCTCACACAAGAAGAAACAGTGTGGATCCAACGATCCAGGGCATTATGGCTGAAAGGAGGGGATAGAAATACTGCATATTTTCACCGACGGGCCTCGAACCGAAAACAAAGAAACAAGATTCTTGGTTTGTTTGACTCAAACAACCAGTGGGTGAATGAAGCAGGGGACATTGAGAGAGTGATCAGTAATTACTACAAACAAATGTTTCGGACTGAAGGAAGCAATGCCAGAGCTCTGGAACTCATCCTCAATACGGTACAGCAGAAAGTGACAAGTAGCATGAATGATCAATTACTAGCCCCATACACTGATGGTGAGATTCGCGCAGCTCTATTTCACATGCACCCATCAAAAGCACCAGGACCGGATGGAATGTCCCCATTTTTCTTCCAAAAATACTGGAACACAGTAGGGCAGGATGTATGCATGGCAGTGCGACACTTCCTTTCTTCTGGAGAAATACTAAGGGAGGTAAATTTTACACATATTACACTCATACCTAAGGTCAAAAATCCGAAACACATGACGGATCTTCGACCTATAGCTTTGTGCAATGTCTTATACAGAATCTGCTCAAAAGTTTTGGCAAATAGACTAAAGAAAATTCTAGCAGATGTTATATCACCTTTGCAAAGTGCCTTTGTCCCAGATCGTTTAATTTCAGACAATATTCTGATAGCAACGGAAGTAGCACATCATCTACACAATAACCGAAGGGGAGGAGATGGACAATTTTCCTTGAAACTGGATATTAGCAAAGCCTACGATAGACTTGAATGGCCTTTCTTACAAGCTATACTTCTGAAACTAGGCTTTGCAGCAGAATGGGTGGATTTGGTTATGGCCAGCATCAAAACAGTCAGCTACTCTTTTTTAGTGAATGGAGAAGTCAGAGGCTATGTAAAACCATCTCGAGGTATAAGACAAGGGGACCCTATATCCCCATACCTATTCATATTATGCGCAGAAGGCTTATCAGCCTTAATTGAGCACTTTGCACAACAACAATGGATCCAGGGTATTGTTCTTGCACCAACTGCACCACGCCTGCACCACCTACTCTTTGCTGATGACAGTTTCTTGTTTGGCACGGCCAACACAGAAGAATGCCAACAGTTCCGAAATATCCTGCACACATATGAAATTGCTTCGGGGCAAAAGATCAACCTCCAGAAAAGTGAAGTAGCATTCAGCAAGAATATTGATAGTACTGTGCAGCATCAATTAGCCAATTTGTTGGGGGTCCAAAGGGTTGACAAGCATGAAAGATATCTTGGATTGCCAACCTATGTGGGACGCACAAAAACAGCGGCTTTTAGCTACCTCAAAGAAAAACTAACTAACAAGGTCGTTAGTTGGCGAGCAAAATTACTGAGTGGAGCTGGCAAGGAGATATTAATCAAAGCGGTGGCACAAACAGTACCAAACTATGTAATGAACTGCTATAAACTGCCAATAGGTTTATGTGAAGACCTACAACAACTGTGTGCAGGTTTTTGGTGGGGAGACTCAGAGGACAAGCAACATATACATTGGAGATCTTGGGATAGATTGTGTGTACCAAAAAAAGAAGGTGGAATGGGATTCAAAAATTTACACTGGTTTAATCTAGGAATGTTGGCAAAACAAGGTTGGCGCATTGTGAGAAATCCGAACTCCCTGGTAGCAAGACTCTACAAAGCTATCTACCATCCAAACAGTGATTTCCTACATGCAGAACTAGGGGATCAACCTTCGTTCCCCTGGAGAAGCATCTGGGAAGGCAGAGCAGTACTCAAACAAGGTTTACGATGGCAGGTGGGCAATGGGCAAAATATTGATATATGGCGGGACTCTTGGCTACCAGACTCCTACCCTCGCAGTCCGACTACTCCGCAACCCAGAGACGCACCGAAAAAGGTATGTGAACTAATCTGTCCACATACAAAGAATTGGAATATACAGAAACTAGAGCAGTGGTTTGCTCCAGTGGATATAGAGCTGATCCTAAAAATACCTCTAAGCAAACGAGCTCCCCGAGACAGGCTAGTTTGGCATTTCAATACCAAGGGCCTATTCACTACAAAAAGCGCTTACTATGTGGCAAGGGACCAAGCGTTGGGGCTGGTTTTAGCTCCCCCACGAATTCCGGATGCATATGACAAGCTATGGCAAGCTATATGGACTGCAAAGGTCCCAAACAAAGTTGCTTTGCAGGTCTGGAGGACATGCGTCAATATTCTACCAAATAGAAGCTGCTTGAGTACAAAAGGATATACAGGAGAATTAACATGCCCATTGTGTGAAAATGGAATGGAAGAAAACATCCACGTATTCACAACTTGCGATTATGCTAAAGAAATCTGGGCAGCAGCGACAATAGCTCGGCCACAGGGTGACTTCGCAGATATAAAAGAATGGCTCCTACGTTCGGTATCCGTGACAAGCAAGGAAGGGTTTGCAAAAATAATGATGCTGATATGGGGAATCTGGAAAAATCGAAACACCCAGGTGTGGGAAGGAAAAAAACAACATCCTCGTGATGTTGTATTGATGACTATGAGCTGGCTAGAGGACTTCCTGAAAGCAAATAACAATGAACATCCTCATAGGGACAGAACCAAACGTTGGGAGAAGCCCCCTGAGACCTGGTTGAAATGCAATGTAGATGGTGCCTTTATAGCACAGGTGGGACTAAGTGGAGCTGGCATGGTATTCAGAGACGATAGAGGCCAGTTTCGAGCAGCAGCTATGAGACCTTTATCAGCAATTACCACACCTTTCCATGCCGAACTGCAGGCCATGCATGAAGCCGCTCAAATGGCAGCTGGAATGAACTACCAAAAAGTCATTTTTGAGACTGACTGTTCTACGCTGGCAGCTGCACTAAATCAAGAAGAACGAGATTGCTCTTTGCATGGTTTCCTGATAGACGATGTCAAAGATTTCATGCACCAACATGGGGAGTATATAATCATCTACGCTCCACGTGAGACCAACAAGATAGCCCACAACTTAGCTAGTAAAGCTTGTAATAGTAATAGCCAAAACTGGTTTGGCACTGCTCCCGTATTTATAAGGGATGCCATTCTAAACGAGTGTTCTCGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002020 GO:0003674 GO:0004857 GO:0004866 GO:0004869 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005623 GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0009892 GO:0009987 GO:0010029 GO:0010030 GO:0010466 GO:0010605 GO:0010951 GO:0019222 GO:0019899 GO:0030162 GO:0030234 GO:0030312 GO:0030414 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0033554 GO:0034605 GO:0043086 GO:0044092 GO:0044464 GO:0045861 GO:0048518 GO:0048519 GO:0048523 GO:0048580 GO:0048582 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0051716 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0065007 GO:0065009 GO:0071944 GO:0080090 GO:0098772 GO:1900140 GO:2000026 GO:2000116 GO:2000117

KEGG Pathways

Metabolic & Signaling

ko04970 map04970

Pfam Domains

Protein Families

SQAPI

Protein Analysis

1364

Amino Acids

156.26

Weight (kDa)

9.08

Isoelectric Point (pI)

41.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Exo_endo_phos	PF03372	7 - 222	6.5e-11	Endonuclease/Exonuclease/phosphatase family
RVT_1	PF00078	507 - 749	9.5e-28	Reverse transcriptase (RNA-dependent DNA polymerase)
zf-RVT	PF13966	1021 - 1117	8.9e-13	zinc-binding in reverse transcriptase
RVT_3	PF13456	1216 - 1338	2.9e-22	Reverse transcriptase-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0025517)

Species	Orthologous Gene IDs
rosa_multiflora	Rmu_sc0001712.1_g000006
rosa_rugosa	Rorug04G0096900
rosa_samantha	Rh1CG096800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	4061
AarI	CACCTGC	2 cut(s)	651, 2791
Acc16I	TGCGCA	1 cut(s)	1969
Acc36I	ACCTGC	5 cut(s)	651, 814, 2486, 2791, 3190
AccB1I	GGYRCC	1 cut(s)	3656
AccB7I	CCANNNNNTGG	2 cut(s)	3103, 3612
AccBSI	CCGCTC	1 cut(s)	3800
AccII	CGCG	1 cut(s)	1329
AccIII	TCCGGA	1 cut(s)	3133
AciI	CCGC	5 cut(s)	2315, 2411, 2833, 2879, 3798
AclI	AACGTT	1 cut(s)	3610
AcoI	YGGCCR	3 cut(s)	1848, 2097, 3371
AcsI	RAATTY	5 cut(s)	992, 1480, 1608, 2596, 3129
AcuI	CTGAAG	3 cut(s)	642, 715, 1239
AcvI	CACGTG	1 cut(s)	3974
AdeI	CACNNNGTG	1 cut(s)	3380
AfaI	GTAC	8 cut(s)	15, 240, 1263, 2215, 2427, 2565, 2778, 3250
AfeI	AGCGCT	1 cut(s)	3076
AgsI	TTSAA	8 cut(s)	424, 485, 998, 1747, 1781, 2593, 3046, 3640
AjnI	CCWGG	8 cut(s)	50, 1040, 1363, 2019, 2664, 2747, 3492, 3632
AjuI	GAANNNNNNNTTGG	6 cut(s)	2262, 2294, 3044, 3076, 3595, 3627
AleI	CACNNNNGTG	1 cut(s)	3494
Alw21I	GWGCWC	5 cut(s)	64, 922, 1240, 1998, 3019
AlwNI	CAGNNNCTG	2 cut(s)	982, 1575
Ama87I	CYCGRG	2 cut(s)	1917, 3021
Aor13HI	TCCGGA	1 cut(s)	3133
Aor51HI	AGCGCT	1 cut(s)	3076
ApoI	RAATTY	5 cut(s)	992, 1480, 1608, 2596, 3129
AseI	ATTAAT	1 cut(s)	2402
Asp700I	GAANNNNTTC	1 cut(s)	253
AspA2I	CCTAGG	1 cut(s)	175
AspLEI	GCGC	5 cut(s)	7, 1331, 1970, 2643, 3077
AspS9I	GGNCC	8 cut(s)	183, 1095, 1367, 1936, 2248, 3055, 3094, 3178
AsuHPI	GGTGA	5 cut(s)	1079, 1168, 1331, 1620, 3392
AsuII	TTCGAA	1 cut(s)	738
AvaI	CYCGRG	2 cut(s)	1917, 3021
AvaII	GGWCC	5 cut(s)	1367, 1936, 2248, 3094, 3178
AvrII	CCTAGG	1 cut(s)	175
AxyI	CCTNAGG	1 cut(s)	1506
BalI	TGGCCA	1 cut(s)	1850
BamHI	GGATCC	2 cut(s)	1029, 2016
BanI	GGYRCC	1 cut(s)	3656
BanII	GRGCYC	3 cut(s)	922, 1240, 3019
BauI	CACGAG	1 cut(s)	3522
BbrPI	CACGTG	1 cut(s)	3974
BbsI	GAAGAC	2 cut(s)	475, 2481
Bbv12I	GWGCWC	5 cut(s)	64, 922, 1240, 1998, 3019
BceAI	ACGGC	1 cut(s)	2112
BciT130I	CCWGG	8 cut(s)	52, 1042, 1365, 2021, 2666, 2749, 3494, 3634
BciVI	GTATCC	1 cut(s)	3430
BclI	TGATCA	4 cut(s)	777, 961, 1185, 1291
BfmI	CTRYAG	3 cut(s)	983, 1545, 3779
BfoI	RGCGCY	2 cut(s)	8, 3078
BfuAI	ACCTGC	5 cut(s)	651, 814, 2486, 2791, 3190
BfuI	GTATCC	1 cut(s)	3430
BglII	AGATCT	1 cut(s)	2543
BlnI	CCTAGG	1 cut(s)	175
BmcAI	AGTACT	3 cut(s)	15, 2215, 2778
Bme1390I	CCNGG	8 cut(s)	52, 1042, 1365, 2021, 2666, 2749, 3494, 3634
Bme18I	GGWCC	5 cut(s)	1367, 1936, 2248, 3094, 3178
BmeT110I	CYCGRG	2 cut(s)	1917, 3021
BmgT120I	GGNCC	8 cut(s)	183, 1095, 1367, 1936, 2248, 3055, 3094, 3178
BmiI	GGNNCC	9 cut(s)	1031, 1937, 1938, 2018, 2249, 3095, 3180, 3407, 3658
BmrFI	CCNGG	8 cut(s)	52, 1042, 1365, 2021, 2666, 2749, 3494, 3634
BmsI	GCATC	9 cut(s)	220, 252, 807, 1863, 2233, 2766, 3127, 3447, 4057
BoxI	GACNNNNGTC	2 cut(s)	666, 3215
BpiI	GAAGAC	2 cut(s)	475, 2481
BplI	GAGNNNNNCTC	4 cut(s)	909, 941, 2763, 2795
BpmI	CTGGAG	5 cut(s)	1479, 2159, 2770, 2955, 3226
Bpu14I	TTCGAA	1 cut(s)	738
BpuEI	CTTGAG	1 cut(s)	3265
BsaAI	YACGTR	3 cut(s)	552, 3316, 3974
BsaBI	GATNNNNATC	1 cut(s)	2402
BsaI	GGTCTC	4 cut(s)	804, 3623, 3733, 3971
BsaJI	CCNNGG	9 cut(s)	175, 202, 401, 1041, 2020, 2664, 2747, 3051, 3492
BsaWI	WCCGGW	2 cut(s)	1369, 3133
Bse1I	ACTGG	8 cut(s)	21, 1150, 1409, 1755, 2609, 2972, 3718, 4040
Bse21I	CCTNAGG	1 cut(s)	1506
Bse3DI	GCAATG	4 cut(s)	1234, 1564, 2815, 3652
Bse8I	GATNNNNATC	1 cut(s)	2402
BseAI	TCCGGA	1 cut(s)	3133
BseBI	CCWGG	8 cut(s)	52, 1042, 1365, 2021, 2666, 2749, 3494, 3634
BseDI	CCNNGG	9 cut(s)	175, 202, 401, 1041, 2020, 2664, 2747, 3051, 3492
BseJI	GATNNNNATC	1 cut(s)	2402
BseMI	GCAATG	4 cut(s)	1234, 1564, 2815, 3652
BseMII	CTCAG	4 cut(s)	644, 2369, 2529, 3618
BseNI	ACTGG	8 cut(s)	21, 1150, 1409, 1755, 2609, 2972, 3718, 4040
BseRI	GAGGAG	1 cut(s)	1740
BsgI	GTGCAG	6 cut(s)	2028, 2039, 2120, 2240, 2514, 3852
Bsh1236I	CGCG	1 cut(s)	1329
BshNI	GGYRCC	1 cut(s)	3656
BsiHKAI	GWGCWC	5 cut(s)	64, 922, 1240, 1998, 3019
BsiHKCI	CYCGRG	2 cut(s)	1917, 3021
BsiSI	CCGG	2 cut(s)	1370, 3134
BsmBI	CGTCTC	4 cut(s)	633, 929, 2883, 3699
BsmI	GAATGC	2 cut(s)	2117, 2192
Bso31I	GGTCTC	4 cut(s)	804, 3623, 3733, 3971
BsoBI	CYCGRG	2 cut(s)	1917, 3021
Bsp119I	TTCGAA	1 cut(s)	738
Bsp1286I	GDGCHC	5 cut(s)	64, 922, 1240, 1998, 3019
Bsp13I	TCCGGA	1 cut(s)	3133
Bsp19I	CCATGG	1 cut(s)	401
BspACI	CCGC	5 cut(s)	2315, 2411, 2833, 2879, 3798
BspCNI	CTCAG	4 cut(s)	643, 2370, 2528, 3619
BspEI	TCCGGA	1 cut(s)	3133
BspFNI	CGCG	1 cut(s)	1329
BspLI	GGNNCC	9 cut(s)	1031, 1937, 1938, 2018, 2249, 3095, 3180, 3407, 3658
BspMAI	CTGCAG	2 cut(s)	987, 3783
BspMI	ACCTGC	5 cut(s)	651, 814, 2486, 2791, 3190
BspQI	GCTCTTC	2 cut(s)	52, 661
BspT104I	TTCGAA	1 cut(s)	738
BspT107I	GGYRCC	1 cut(s)	3656
BspTNI	GGTCTC	4 cut(s)	804, 3623, 3733, 3971
BsrBI	CCGCTC	1 cut(s)	3800
BsrDI	GCAATG	4 cut(s)	1234, 1564, 2815, 3652
BsrI	ACTGG	8 cut(s)	21, 1150, 1409, 1755, 2609, 2972, 3718, 4040
BssECI	CCNNGG	9 cut(s)	175, 202, 401, 1041, 2020, 2664, 2747, 3051, 3492
BssSI	CACGAG	1 cut(s)	3522
BssT1I	CCWWGG	4 cut(s)	175, 202, 401, 3051
Bst2BI	CACGAG	1 cut(s)	3522
Bst2UI	CCWGG	8 cut(s)	52, 1042, 1365, 2021, 2666, 2749, 3494, 3634
Bst6I	CTCTTC	2 cut(s)	52, 661
BstAPI	GCANNNNNTGC	2 cut(s)	2042, 3787
BstBAI	YACGTR	3 cut(s)	552, 3316, 3974
BstBI	TTCGAA	1 cut(s)	738
BstDSI	CCRYGG	1 cut(s)	401
BstFNI	CGCG	1 cut(s)	1329
BstH2I	RGCGCY	2 cut(s)	8, 3078
BstHHI	GCGC	5 cut(s)	7, 1331, 1970, 2643, 3077
BstNI	CCWGG	8 cut(s)	52, 1042, 1365, 2021, 2666, 2749, 3494, 3634
BstNSI	RCATGY	5 cut(s)	269, 1348, 2720, 3216, 3279
BstPAI	GACNNNNGTC	2 cut(s)	666, 3215
BstSCI	CCNGG	8 cut(s)	50, 1040, 1363, 2019, 2664, 2747, 3492, 3632
BstSFI	CTRYAG	3 cut(s)	983, 1545, 3779
BstUI	CGCG	1 cut(s)	1329
BstV2I	GAAGAC	2 cut(s)	475, 2481
BstX2I	RGATCY	5 cut(s)	364, 1029, 1534, 2016, 2543
BstXI	CCANNNNNNTGG	3 cut(s)	552, 1240, 2242
BstYI	RGATCY	5 cut(s)	364, 1029, 1534, 2016, 2543
Bsu36I	CCTNAGG	1 cut(s)	1506
BsuI	GTATCC	1 cut(s)	3430
BtgI	CCRYGG	1 cut(s)	401
BtsI	GCAGTG	4 cut(s)	918, 1443, 2966, 4044
BveI	ACCTGC	5 cut(s)	651, 814, 2486, 2791, 3190
CaiI	CAGNNNCTG	2 cut(s)	982, 1575
CfoI	GCGC	5 cut(s)	7, 1331, 1970, 2643, 3077
Cfr13I	GGNCC	8 cut(s)	183, 1095, 1367, 1936, 2248, 3055, 3094, 3178
CseI	GACGC	4 cut(s)	962, 2310, 2900, 3205
CsiI	ACCWGGT	1 cut(s)	3632
Csp6I	GTAC	8 cut(s)	14, 239, 1262, 2214, 2426, 2564, 2777, 3249
CspCI	CAANNNNNGTGG	4 cut(s)	249, 284, 2277, 2312
CviQI	GTAC	8 cut(s)	14, 239, 1262, 2214, 2426, 2564, 2777, 3249
DraIII	CACNNNGTG	1 cut(s)	3380
EaeI	YGGCCR	3 cut(s)	1848, 2097, 3371
Eam1104I	CTCTTC	2 cut(s)	52, 661
EarI	CTCTTC	2 cut(s)	52, 661
Ecl136II	GAGCTC	3 cut(s)	920, 1238, 3017
Eco130I	CCWWGG	4 cut(s)	175, 202, 401, 3051
Eco24I	GRGCYC	3 cut(s)	922, 1240, 3019
Eco31I	GGTCTC	4 cut(s)	804, 3623, 3733, 3971
Eco32I	GATATC	1 cut(s)	2276
Eco47I	GGWCC	5 cut(s)	1367, 1936, 2248, 3094, 3178
Eco47III	AGCGCT	1 cut(s)	3076
Eco53kI	GAGCTC	3 cut(s)	920, 1238, 3017
Eco57I	CTGAAG	3 cut(s)	642, 715, 1239
Eco72I	CACGTG	1 cut(s)	3974
Eco81I	CCTNAGG	1 cut(s)	1506
Eco88I	CYCGRG	2 cut(s)	1917, 3021
EcoICRI	GAGCTC	3 cut(s)	920, 1238, 3017
EcoO109I	RGGNCCY	3 cut(s)	1936, 3055, 3178
EcoRI	GAATTC	1 cut(s)	3129
EcoRII	CCWGG	8 cut(s)	50, 1040, 1363, 2019, 2664, 2747, 3492, 3632
EcoRV	GATATC	1 cut(s)	2276
EcoT14I	CCWWGG	4 cut(s)	175, 202, 401, 3051
EcoT22I	ATGCAT	3 cut(s)	1433, 3142, 3792
EcoT38I	GRGCYC	3 cut(s)	922, 1240, 3019
ErhI	CCWWGG	4 cut(s)	175, 202, 401, 3051
Esp3I	CGTCTC	4 cut(s)	633, 929, 2883, 3699
FalI	AAGNNNNNCTT	4 cut(s)	931, 963, 1788, 1820
FauI	CCCGC	1 cut(s)	2826
FauNDI	CATATG	2 cut(s)	2143, 3142
FbaI	TGATCA	4 cut(s)	777, 961, 1185, 1291
FriOI	GRGCYC	3 cut(s)	922, 1240, 3019
FspI	TGCGCA	1 cut(s)	1969
GlaI	GCGC	5 cut(s)	6, 1330, 1969, 2642, 3076
GsuI	CTGGAG	5 cut(s)	1479, 2159, 2770, 2955, 3226
HaeII	RGCGCY	2 cut(s)	8, 3078
HapII	CCGG	2 cut(s)	1370, 3134
HgaI	GACGC	4 cut(s)	962, 2310, 2900, 3205
HhaI	GCGC	5 cut(s)	7, 1331, 1970, 2643, 3077
Hin6I	GCGC	5 cut(s)	5, 1329, 1968, 2641, 3075
HinP1I	GCGC	5 cut(s)	5, 1329, 1968, 2641, 3075
HincII	GTYRAC	1 cut(s)	2260
HindII	GTYRAC	1 cut(s)	2260
HindIII	AAGCTT	3 cut(s)	486, 589, 4011
HpaII	CCGG	2 cut(s)	1370, 3134
HphI	GGTGA	5 cut(s)	1079, 1168, 1331, 1620, 3392
Hpy166II	GTNNAC	6 cut(s)	716, 2260, 2564, 2792, 2912, 2924
Hpy8I	GTNNAC	6 cut(s)	716, 2260, 2564, 2792, 2912, 2924
Hpy99I	CGWCG	1 cut(s)	1095
HpyAV	CCTTC	9 cut(s)	739, 1214, 1713, 1967, 2570, 2748, 2759, 3431, 3497
HpyCH4IV	ACGT	7 cut(s)	551, 600, 835, 3315, 3412, 3610, 3973
HpySE526I	ACGT	7 cut(s)	551, 600, 835, 3315, 3412, 3610, 3973
HspAI	GCGC	5 cut(s)	5, 1329, 1968, 2641, 3075
KflI	GGGWCCC	1 cut(s)	1936
Kpn2I	TCCGGA	1 cut(s)	3133
Ksp22I	TGATCA	4 cut(s)	777, 961, 1185, 1291
LguI	GCTCTTC	2 cut(s)	52, 661
LmnI	GCTCC	8 cut(s)	2384, 2974, 3022, 3124, 3411, 3686, 3973, 4054
LweI	GCATC	9 cut(s)	220, 252, 807, 1863, 2233, 2766, 3127, 3447, 4057
MabI	ACCWGGT	1 cut(s)	3632
MaeII	ACGT	7 cut(s)	551, 600, 835, 3315, 3412, 3610, 3973
MaeIII	GTNAC	4 cut(s)	122, 1273, 3380, 3424
MbiI	CCGCTC	1 cut(s)	3800
MflI	RGATCY	5 cut(s)	364, 1029, 1534, 2016, 2543
MhlI	GDGCHC	5 cut(s)	64, 922, 1240, 1998, 3019
MlsI	TGGCCA	1 cut(s)	1850
MluNI	TGGCCA	1 cut(s)	1850
MlyI	GAGTC	5 cut(s)	1133, 2507, 2670, 2831, 2846
MmeI	TCCRAC	5 cut(s)	537, 848, 916, 1057, 2894
Mox20I	TGGCCA	1 cut(s)	1850
Mph1103I	ATGCAT	3 cut(s)	1433, 3142, 3792
MroI	TCCGGA	1 cut(s)	3133
MroXI	GAANNNNTTC	1 cut(s)	253
MscI	TGGCCA	1 cut(s)	1850
MseI	TTAA	6 cut(s)	840, 1661, 1988, 2402, 2610, 3272
MslI	CAYNNNNRTG	7 cut(s)	270, 550, 1436, 2142, 3494, 3522, 3942
Msp20I	TGGCCA	1 cut(s)	1850
MspA1I	CMGCKG	4 cut(s)	982, 2315, 3812, 3866
MspI	CCGG	2 cut(s)	1370, 3134
MspR9I	CCNGG	8 cut(s)	52, 1042, 1365, 2021, 2666, 2749, 3494, 3634
Mva1269I	GAATGC	2 cut(s)	2117, 2192
MvaI	CCWGG	8 cut(s)	52, 1042, 1365, 2021, 2666, 2749, 3494, 3634
MvnI	CGCG	1 cut(s)	1329
NcoI	CCATGG	1 cut(s)	401
NdeI	CATATG	2 cut(s)	2143, 3142
NlaIV	GGNNCC	9 cut(s)	1031, 1937, 1938, 2018, 2249, 3095, 3180, 3407, 3658
NmeAIII	GCCGAG	1 cut(s)	3349
NmuCI	GTSAC	4 cut(s)	122, 1273, 3380, 3424
NsbI	TGCGCA	1 cut(s)	1969
NsiI	ATGCAT	3 cut(s)	1433, 3142, 3792
NspI	RCATGY	5 cut(s)	269, 1348, 2720, 3216, 3279
NspV	TTCGAA	1 cut(s)	738
OliI	CACNNNNGTG	1 cut(s)	3494
PaeR7I	CTCGAG	1 cut(s)	1917
PaqCI	CACCTGC	2 cut(s)	651, 2791
PciSI	GCTCTTC	2 cut(s)	52, 661
PcsI	WCGNNNNNNNCGW	1 cut(s)	3019
PctI	GAATGC	2 cut(s)	2117, 2192
PdmI	GAANNNNTTC	1 cut(s)	253
PfeI	GAWTC	9 cut(s)	150, 162, 481, 735, 1123, 1324, 1572, 2589, 3471
PflFI	GACNNNGTC	1 cut(s)	3920
PflMI	CCANNNNNTGG	2 cut(s)	3103, 3612
PfoI	TCCNGGA	1 cut(s)	50
PleI	GAGTC	5 cut(s)	1133, 2507, 2670, 2831, 2846
PmaCI	CACGTG	1 cut(s)	3974
PmlI	CACGTG	1 cut(s)	3974
PpsI	GAGTC	5 cut(s)	1133, 2507, 2670, 2831, 2846
Ppu21I	YACGTR	3 cut(s)	552, 3316, 3974
PpuMI	RGGWCCY	2 cut(s)	1936, 3178
PshAI	GACNNNNGTC	2 cut(s)	666, 3215
PshBI	ATTAAT	1 cut(s)	2402
PsiI	TTATAA	1 cut(s)	4061
Psp124BI	GAGCTC	3 cut(s)	922, 1240, 3019
Psp1406I	AACGTT	1 cut(s)	3610
Psp5II	RGGWCCY	2 cut(s)	1936, 3178
Psp6I	CCWGG	8 cut(s)	50, 1040, 1363, 2019, 2664, 2747, 3492, 3632
PspCI	CACGTG	1 cut(s)	3974
PspGI	CCWGG	8 cut(s)	50, 1040, 1363, 2019, 2664, 2747, 3492, 3632
PspN4I	GGNNCC	9 cut(s)	1031, 1937, 1938, 2018, 2249, 3095, 3180, 3407, 3658
PspPI	GGNCC	8 cut(s)	183, 1095, 1367, 1936, 2248, 3055, 3094, 3178
PspPPI	RGGWCCY	2 cut(s)	1936, 3178
PstI	CTGCAG	2 cut(s)	987, 3783
PstNI	CAGNNNCTG	2 cut(s)	982, 1575
PsuI	RGATCY	5 cut(s)	364, 1029, 1534, 2016, 2543
PsyI	GACNNNGTC	1 cut(s)	3920
PvuII	CAGCTG	3 cut(s)	982, 3812, 3866
RsaI	GTAC	8 cut(s)	15, 240, 1263, 2215, 2427, 2565, 2778, 3250
RsaNI	GTAC	8 cut(s)	14, 239, 1262, 2214, 2426, 2564, 2777, 3249
RseI	CAYNNNNRTG	7 cut(s)	270, 550, 1436, 2142, 3494, 3522, 3942
SacI	GAGCTC	3 cut(s)	922, 1240, 3019
SapI	GCTCTTC	2 cut(s)	52, 661
SaqAI	TTAA	6 cut(s)	840, 1661, 1988, 2402, 2610, 3272
Sau96I	GGNCC	8 cut(s)	183, 1095, 1367, 1936, 2248, 3055, 3094, 3178
ScaI	AGTACT	3 cut(s)	15, 2215, 2778
SchI	GAGTC	5 cut(s)	1133, 2507, 2670, 2831, 2846
ScrFI	CCNGG	8 cut(s)	52, 1042, 1365, 2021, 2666, 2749, 3494, 3634
SduI	GDGCHC	5 cut(s)	64, 922, 1240, 1998, 3019
SexAI	ACCWGGT	1 cut(s)	3632
SfaNI	GCATC	9 cut(s)	220, 252, 807, 1863, 2233, 2766, 3127, 3447, 4057
SfcI	CTRYAG	3 cut(s)	983, 1545, 3779
Sfr274I	CTCGAG	1 cut(s)	1917
SfuI	TTCGAA	1 cut(s)	738
SinI	GGWCC	5 cut(s)	1367, 1936, 2248, 3094, 3178
SlaI	CTCGAG	1 cut(s)	1917
SmiMI	CAYNNNNRTG	7 cut(s)	270, 550, 1436, 2142, 3494, 3522, 3942
SmlI	CTYRAG	2 cut(s)	1917, 3244
SmoI	CTYRAG	2 cut(s)	1917, 3244
SsiI	CCGC	5 cut(s)	2315, 2411, 2833, 2879, 3798
SspI	AATATT	4 cut(s)	1675, 2206, 2821, 3223
SstI	GAGCTC	3 cut(s)	922, 1240, 3019
StyD4I	CCNGG	8 cut(s)	50, 1040, 1363, 2019, 2664, 2747, 3492, 3632
StyI	CCWWGG	4 cut(s)	175, 202, 401, 3051
TaiI	ACGT	7 cut(s)	554, 603, 838, 3318, 3415, 3613, 3976
TatI	WGTACW	4 cut(s)	13, 2213, 2776, 3248
TauI	GCSGC	2 cut(s)	2318, 3800
TfiI	GAWTC	9 cut(s)	150, 162, 481, 735, 1123, 1324, 1572, 2589, 3471
Tru1I	TTAA	6 cut(s)	840, 1661, 1988, 2402, 2610, 3272
Tru9I	TTAA	6 cut(s)	840, 1661, 1988, 2402, 2610, 3272
TseFI	GTSAC	4 cut(s)	122, 1273, 3380, 3424
Tsp45I	GTSAC	4 cut(s)	122, 1273, 3380, 3424
TspGWI	ACGGA	3 cut(s)	1547, 1703, 3412
Tth111I	GACNNNGTC	1 cut(s)	3920
Van91I	CCANNNNNTGG	2 cut(s)	3103, 3612
VpaK11BI	GGWCC	5 cut(s)	1367, 1936, 2248, 3094, 3178
VspI	ATTAAT	1 cut(s)	2402
XapI	RAATTY	5 cut(s)	992, 1480, 1608, 2596, 3129
XceI	RCATGY	5 cut(s)	269, 1348, 2720, 3216, 3279
XcmI	CCANNNNNNNNNTGG	2 cut(s)	577, 4037
XhoI	CTCGAG	1 cut(s)	1917
XmaJI	CCTAGG	1 cut(s)	175
XmnI	GAANNNNTTC	1 cut(s)	253
ZrmI	AGTACT	3 cut(s)	15, 2215, 2778
Zsp2I	ATGCAT	3 cut(s)	1433, 3142, 3792

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.