Rorug04G0381900

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0007958
Physical Location & Seq
Reverse (-)
55248656 .. 55262212
13557 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0381900.1

Sequence Viewer

Length: 1428 bp
ATGGCTTCACAGGGAATCAGAGGACCTCCTTACAGACTTATCGATGGAAACGCCAAAGAAGTCTCCAACATGGGCAAGGAAGTCATGAGCAGGCCCTTGGATCTGTCACACAACATATTTCCAGTAGTTCTACCTCATATTCATGCATGGACCAAAATCTATGGGAAGAATTATCTTCAGTGGCATGGTACTCTACCTCAGTTGGTGGTGACGGAAGCTGAGTTGTGCAAGGAGATATTCACTAACAAAGATGGAGTTTATCTGAAACCCAAGTTCAGACCTTATGCAAAGAAGGTATTTGGGAATGGCCTTCCGGCATTAGAAGGTGAGAAATGGGCGAAATCGCGAAGGCTGGCCAACCATGCCTTCCATGGAGAGAGCTTAAAAAATATGATTCCAGACATGATAGCTAGTGCCGAGACAATGCTAGACAGGTGGAAAAGCCATGAAGAAGGGAAAGAAATGGAAGTGTATGAAGAATTTAGATTATTAACTTCAGAAGTGATTTCTAGGACAGCATTTGGCAGCAGCTATGTAGAAGGGAAGAACATTTTTGATATGTTGATGAAGTTTAGCTTATTGCTCAAAAATGATTACATACTCAAGTTTCCAGGCATCAGCAAGCTTTTTAAAACCAACGATGATATCAAATCAGAGAAGCTTGCGCAAGGAATACGTGACTCCATAATGGGGATCATTAAGAAAAGAGAAGAGAAGGCAATGAGTGGGGAAGAAGAGAGATTTGGGAGTGATTTTCTTGGTTTACTATTGAAGGCTCATCATGATACCAATGACATTCAGAGGATTTCGGTGGATGATTTGGTTGATGAGTGCAAGTCATTTTACTTGGTTGGACAAGAGACCAGTAATACTTTGCTTTCCTGGACTGTCTTGCTTTTGGCACTCCATACTGATTGGCAAGAGAAAGCAAGAATGGAAGTCCTACAATTATTTGGGAAACAGGCTCCGAATCCTGATGGCCTTGCCAAATTGAAGACCATGAGTATGATCTTCAATGAGTCCCTAAGGCTATATTCTCCAGCTGTTACCGTCACAAGGAGAGTTGAAAAGGATGTTAGACTCGGAAAGCTCATTGTTCCTGCTAATTTTGAGTTGATTGTCCCATATCTGGCGCTTCACCACGAGCCTCAGTCATGGGGACAAGACGCCCAACTTTTCAAACCAGAGCGATTCTTTGAAGGAGTTGCTAAAGCTACTAACAACAACTTAACTTCATTTATGCCCTTTGGAATGGGACCTAGAATTTGTGTGGGCCTCAACTTCGCCACCGTTGAAGCCAAGATTGCTCTATCAATGATTCTACAGCGCTACTCCTTTACCCTTTCCCCAGGTTATGTTCACTCACCCTTAAGGAGTGTGACACTTCGCCCACAGCATGGGGTTCAAGTAATGCTACACTCATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

475

Amino Acids

53.96

Weight (kDa)

8.9

Isoelectric Point (pI)

36.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 50 - 451 2.6e-69 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 666
AccB7I CCANNNNNTGG 1 cut(s) 1397
AccII CGCG 1 cut(s) 346
AclWI GGATC 2 cut(s) 108, 701
AcoI YGGCCR 1 cut(s) 354
AcsI RAATTY 2 cut(s) 479, 1263
AcuI CTGAAG 2 cut(s) 161, 480
AcyI GRCGYC 1 cut(s) 1167
AfaI GTAC 1 cut(s) 190
AfeI AGCGCT 1 cut(s) 1328
AfiI CCNNNNNNNGG 4 cut(s) 690, 1056, 1129, 1397
AflII CTTAAG 1 cut(s) 1369
AgsI TTSAA 8 cut(s) 772, 994, 1015, 1067, 1180, 1199, 1295, 1406
AjnI CCWGG 3 cut(s) 610, 881, 1348
AjuI GAANNNNNNNTTGG 4 cut(s) 350, 382, 726, 758
Alw26I GTCTC 3 cut(s) 67, 413, 854
AlwI GGATC 2 cut(s) 108, 701
Aor51HI AGCGCT 1 cut(s) 1328
AoxI GGCC 5 cut(s) 92, 307, 354, 979, 1273
ApeKI GCWGC 2 cut(s) 525, 528
ApoI RAATTY 2 cut(s) 479, 1263
ArsI GACNNNNNNTTYG 2 cut(s) 1248, 1280
AspLEI GCGC 3 cut(s) 667, 1135, 1329
AspS9I GGNCC 5 cut(s) 23, 93, 150, 1256, 1273
AsuHPI GGTGA 4 cut(s) 220, 338, 1130, 1356
AvaII GGWCC 3 cut(s) 23, 150, 1256
AxyI CCTNAGG 1 cut(s) 1025
BalI TGGCCA 1 cut(s) 356
BauI CACGAG 1 cut(s) 1142
BbsI GAAGAC 1 cut(s) 1001
BbvI GCAGC 2 cut(s) 537, 540
BccI CCATC 3 cut(s) 38, 245, 971
BciT130I CCWGG 3 cut(s) 612, 883, 1350
BcoDI GTCTC 3 cut(s) 67, 413, 854
BfaI CTAG 4 cut(s) 411, 428, 510, 1260
BfmI CTRYAG 1 cut(s) 1322
BfoI RGCGCY 2 cut(s) 1136, 1330
BfrI CTTAAG 1 cut(s) 1369
BisI GCNGC 2 cut(s) 526, 529
BlsI GCNGC 2 cut(s) 527, 530
Bme1390I CCNGG 3 cut(s) 612, 883, 1350
Bme18I GGWCC 3 cut(s) 23, 150, 1256
BmgT120I GGNCC 5 cut(s) 23, 93, 150, 1256, 1273
BmiI GGNNCC 2 cut(s) 966, 1257
BmrFI CCNGG 3 cut(s) 612, 883, 1350
BmsI GCATC 1 cut(s) 624
BpiI GAAGAC 1 cut(s) 1001
BpmI CTGGAG 1 cut(s) 1023
BpuEI CTTGAG 1 cut(s) 587
Bsa29I ATCGAT 1 cut(s) 42
BsaAI YACGTR 1 cut(s) 677
BsaHI GRCGYC 1 cut(s) 1167
BsaI GGTCTC 1 cut(s) 854
BsaJI CCNNGG 3 cut(s) 96, 370, 1348
Bsc4I CCNNNNNNNGG 4 cut(s) 690, 1056, 1129, 1397
Bse1I ACTGG 2 cut(s) 122, 864
Bse21I CCTNAGG 1 cut(s) 1025
Bse3DI GCAATG 1 cut(s) 726
BseBI CCWGG 3 cut(s) 612, 883, 1350
BseCI ATCGAT 1 cut(s) 42
BseDI CCNNGG 3 cut(s) 96, 370, 1348
BseGI GGATG 2 cut(s) 820, 1078
BseLI CCNNNNNNNGG 4 cut(s) 690, 1056, 1129, 1397
BseMI GCAATG 1 cut(s) 726
BseMII CTCAG 3 cut(s) 210, 212, 1163
BseNI ACTGG 2 cut(s) 122, 864
BseXI GCAGC 2 cut(s) 537, 540
Bsh1236I CGCG 1 cut(s) 346
BshFI GGCC 5 cut(s) 94, 309, 356, 981, 1275
BshVI ATCGAT 1 cut(s) 42
BsiSI CCGG 1 cut(s) 314
BslFI GGGAC 4 cut(s) 1006, 1106, 1173, 1269
BslI CCNNNNNNNGG 4 cut(s) 690, 1056, 1129, 1397
BsmAI GTCTC 3 cut(s) 67, 413, 854
BsmFI GGGAC 4 cut(s) 1006, 1106, 1173, 1269
BsnI GGCC 5 cut(s) 94, 309, 356, 981, 1275
Bso31I GGTCTC 1 cut(s) 854
Bsp143I GATC 3 cut(s) 100, 693, 1008
Bsp19I CCATGG 1 cut(s) 370
Bsp68I TCGCGA 1 cut(s) 346
BspANI GGCC 5 cut(s) 94, 309, 356, 981, 1275
BspCNI CTCAG 3 cut(s) 211, 211, 1162
BspDI ATCGAT 1 cut(s) 42
BspFNI CGCG 1 cut(s) 346
BspHI TCATGA 2 cut(s) 84, 781
BspLI GGNNCC 2 cut(s) 966, 1257
BspPI GGATC 2 cut(s) 108, 701
BspTI CTTAAG 1 cut(s) 1369
BspTNI GGTCTC 1 cut(s) 854
BsrDI GCAATG 1 cut(s) 726
BsrI ACTGG 2 cut(s) 122, 864
BssECI CCNNGG 3 cut(s) 96, 370, 1348
BssMI GATC 3 cut(s) 100, 693, 1008
BssNI GRCGYC 1 cut(s) 1167
BssSI CACGAG 1 cut(s) 1142
BssT1I CCWWGG 2 cut(s) 96, 370
Bst2BI CACGAG 1 cut(s) 1142
Bst2UI CCWGG 3 cut(s) 612, 883, 1350
Bst4CI ACNGT 3 cut(s) 889, 1051, 1291
Bst6I CTCTTC 2 cut(s) 705, 729
BstACI GRCGYC 1 cut(s) 1167
BstAFI CTTAAG 1 cut(s) 1369
BstBAI YACGTR 1 cut(s) 677
BstC8I GCNNGC 4 cut(s) 92, 354, 623, 663
BstDEI CTNAG 4 cut(s) 198, 219, 1025, 1149
BstDSI CCRYGG 1 cut(s) 370
BstF5I GGATG 2 cut(s) 820, 1078
BstFNI CGCG 1 cut(s) 346
BstH2I RGCGCY 2 cut(s) 1136, 1330
BstHHI GCGC 3 cut(s) 667, 1135, 1329
BstKTI GATC 3 cut(s) 103, 696, 1011
BstMAI GTCTC 3 cut(s) 67, 413, 854
BstMBI GATC 3 cut(s) 100, 693, 1008
BstMWI GCNNNNNNNGC 2 cut(s) 362, 1304
BstNI CCWGG 3 cut(s) 612, 883, 1350
BstSCI CCNGG 3 cut(s) 610, 881, 1348
BstSFI CTRYAG 1 cut(s) 1322
BstUI CGCG 1 cut(s) 346
BstV1I GCAGC 2 cut(s) 537, 540
BstV2I GAAGAC 1 cut(s) 1001
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
Bsu15I ATCGAT 1 cut(s) 42
Bsu36I CCTNAGG 1 cut(s) 1025
BsuRI GGCC 5 cut(s) 94, 309, 356, 981, 1275
BsuTUI ATCGAT 1 cut(s) 42
BtgI CCRYGG 1 cut(s) 370
BtsCI GGATG 2 cut(s) 820, 1078
BtsIMutI CAGTG 1 cut(s) 185
BtuMI TCGCGA 1 cut(s) 346
Cac8I GCNNGC 4 cut(s) 92, 354, 623, 663
CciI TCATGA 2 cut(s) 84, 781
CfoI GCGC 3 cut(s) 667, 1135, 1329
Cfr13I GGNCC 5 cut(s) 23, 93, 150, 1256, 1273
ClaI ATCGAT 1 cut(s) 42
CseI GACGC 1 cut(s) 1175
Csp6I GTAC 1 cut(s) 189
CviQI GTAC 1 cut(s) 189
DdeI CTNAG 4 cut(s) 198, 219, 1025, 1149
DpnI GATC 3 cut(s) 102, 695, 1010
DpnII GATC 3 cut(s) 100, 693, 1008
DraI TTTAAA 1 cut(s) 631
EaeI YGGCCR 1 cut(s) 354
Eam1104I CTCTTC 2 cut(s) 705, 729
EarI CTCTTC 2 cut(s) 705, 729
Eco130I CCWWGG 2 cut(s) 96, 370
Eco31I GGTCTC 1 cut(s) 854
Eco32I GATATC 1 cut(s) 646
Eco47I GGWCC 3 cut(s) 23, 150, 1256
Eco47III AGCGCT 1 cut(s) 1328
Eco57I CTGAAG 2 cut(s) 161, 480
Eco81I CCTNAGG 1 cut(s) 1025
EcoO109I RGGNCCY 3 cut(s) 23, 93, 1256
EcoRII CCWGG 3 cut(s) 610, 881, 1348
EcoRV GATATC 1 cut(s) 646
EcoT14I CCWWGG 2 cut(s) 96, 370
EcoT22I ATGCAT 1 cut(s) 148
ErhI CCWWGG 2 cut(s) 96, 370
FalI AAGNNNNNCTT 2 cut(s) 560, 592
FaqI GGGAC 4 cut(s) 1006, 1106, 1173, 1269
Fnu4HI GCNGC 2 cut(s) 526, 529
FokI GGATG 2 cut(s) 827, 1085
Fsp4HI GCNGC 2 cut(s) 526, 529
FspBI CTAG 4 cut(s) 411, 428, 510, 1260
FspI TGCGCA 1 cut(s) 666
GlaI GCGC 3 cut(s) 666, 1134, 1328
GluI GCNGC 2 cut(s) 526, 529
GsuI CTGGAG 1 cut(s) 1023
HaeII RGCGCY 2 cut(s) 1136, 1330
HaeIII GGCC 5 cut(s) 94, 309, 356, 981, 1275
HapII CCGG 1 cut(s) 314
HgaI GACGC 1 cut(s) 1175
HhaI GCGC 3 cut(s) 667, 1135, 1329
Hin1I GRCGYC 1 cut(s) 1167
Hin6I GCGC 3 cut(s) 665, 1133, 1327
HinP1I GCGC 3 cut(s) 665, 1133, 1327
HindIII AAGCTT 2 cut(s) 623, 659
HinfI GANTC 8 cut(s) 15, 394, 680, 970, 1019, 1080, 1191, 1318
HpaII CCGG 1 cut(s) 314
HphI GGTGA 4 cut(s) 220, 338, 1130, 1356
Hpy166II GTNNAC 2 cut(s) 764, 1360
Hpy188I TCNGA 8 cut(s) 20, 264, 278, 499, 655, 801, 969, 1085
Hpy188III TCNNGA 5 cut(s) 85, 345, 398, 782, 974
Hpy8I GTNNAC 2 cut(s) 764, 1360
HpyCH4III ACNGT 3 cut(s) 889, 1051, 1291
HpyCH4IV ACGT 1 cut(s) 676
HpyCH4V TGCA 4 cut(s) 146, 228, 287, 834
HpyF10VI GCNNNNNNNGC 2 cut(s) 362, 1304
HpyF3I CTNAG 4 cut(s) 198, 219, 1025, 1149
HpySE526I ACGT 1 cut(s) 676
Hsp92I GRCGYC 1 cut(s) 1167
HspAI GCGC 3 cut(s) 665, 1133, 1327
Kzo9I GATC 3 cut(s) 100, 693, 1008
LmnI GCTCC 1 cut(s) 970
Lsp1109I GCAGC 2 cut(s) 537, 540
LweI GCATC 1 cut(s) 624
MaeI CTAG 4 cut(s) 411, 428, 510, 1260
MaeII ACGT 1 cut(s) 676
MaeIII GTNAC 6 cut(s) 105, 208, 677, 1045, 1051, 1378
MalI GATC 3 cut(s) 102, 695, 1010
MboI GATC 3 cut(s) 100, 693, 1008
MflI RGATCY 1 cut(s) 100
MlsI TGGCCA 1 cut(s) 356
MluCI AATT 6 cut(s) 169, 479, 947, 989, 1105, 1263
MluNI TGGCCA 1 cut(s) 356
MlyI GAGTC 3 cut(s) 674, 1028, 1074
MmeI TCCRAC 2 cut(s) 90, 832
MnlI CCTC 7 cut(s) 14, 36, 144, 207, 795, 1158, 1286
Mox20I TGGCCA 1 cut(s) 356
Mph1103I ATGCAT 1 cut(s) 148
MscI TGGCCA 1 cut(s) 356
MseI TTAA 6 cut(s) 383, 491, 630, 699, 1229, 1370
MslI CAYNNNNRTG 2 cut(s) 141, 1004
Msp20I TGGCCA 1 cut(s) 356
MspA1I CMGCKG 1 cut(s) 1043
MspCI CTTAAG 1 cut(s) 1369
MspI CCGG 1 cut(s) 314
MspR9I CCNGG 3 cut(s) 612, 883, 1350
MvaI CCWGG 3 cut(s) 612, 883, 1350
MvnI CGCG 1 cut(s) 346
MwoI GCNNNNNNNGC 2 cut(s) 362, 1304
NcoI CCATGG 1 cut(s) 370
NdeII GATC 3 cut(s) 100, 693, 1008
NlaIV GGNNCC 2 cut(s) 966, 1257
NmeAIII GCCGAG 1 cut(s) 442
NmuCI GTSAC 5 cut(s) 105, 208, 677, 1051, 1378
NruI TCGCGA 1 cut(s) 346
NsbI TGCGCA 1 cut(s) 666
NsiI ATGCAT 1 cut(s) 148
PagI TCATGA 2 cut(s) 84, 781
PfeI GAWTC 5 cut(s) 15, 394, 970, 1191, 1318
PflMI CCANNNNNTGG 1 cut(s) 1397
PfoI TCCNGGA 1 cut(s) 881
PkrI GCNGC 2 cut(s) 527, 530
PleI GAGTC 3 cut(s) 674, 1027, 1074
PpsI GAGTC 3 cut(s) 674, 1027, 1074
Ppu21I YACGTR 1 cut(s) 677
PpuMI RGGWCCY 2 cut(s) 23, 1256
Psp5II RGGWCCY 2 cut(s) 23, 1256
Psp6I CCWGG 3 cut(s) 610, 881, 1348
PspGI CCWGG 3 cut(s) 610, 881, 1348
PspN4I GGNNCC 2 cut(s) 966, 1257
PspPI GGNCC 5 cut(s) 23, 93, 150, 1256, 1273
PspPPI RGGWCCY 2 cut(s) 23, 1256
PsuI RGATCY 1 cut(s) 100
PvuII CAGCTG 1 cut(s) 1043
RruI TCGCGA 1 cut(s) 346
RsaI GTAC 1 cut(s) 190
RsaNI GTAC 1 cut(s) 189
RseI CAYNNNNRTG 2 cut(s) 141, 1004
SaqAI TTAA 6 cut(s) 383, 491, 630, 699, 1229, 1370
SatI GCNGC 2 cut(s) 526, 529
Sau3AI GATC 3 cut(s) 100, 693, 1008
Sau96I GGNCC 5 cut(s) 23, 93, 150, 1256, 1273
SchI GAGTC 3 cut(s) 674, 1028, 1074
ScrFI CCNGG 3 cut(s) 612, 883, 1350
SfaNI GCATC 1 cut(s) 624
SfcI CTRYAG 1 cut(s) 1322
SinI GGWCC 3 cut(s) 23, 150, 1256
SmiMI CAYNNNNRTG 2 cut(s) 141, 1004
SmlI CTYRAG 2 cut(s) 602, 1369
SmoI CTYRAG 2 cut(s) 602, 1369
Sse9I AATT 6 cut(s) 169, 479, 947, 989, 1105, 1263
SspMI CTAG 4 cut(s) 411, 428, 510, 1260
StyD4I CCNGG 3 cut(s) 610, 881, 1348
StyI CCWWGG 2 cut(s) 96, 370
TaaI ACNGT 3 cut(s) 889, 1051, 1291
TaiI ACGT 1 cut(s) 679
TaqI TCGA 1 cut(s) 42
TasI AATT 6 cut(s) 169, 479, 947, 989, 1105, 1263
TfiI GAWTC 5 cut(s) 15, 394, 970, 1191, 1318
Tru1I TTAA 6 cut(s) 383, 491, 630, 699, 1229, 1370
Tru9I TTAA 6 cut(s) 383, 491, 630, 699, 1229, 1370
TscAI CASTG 1 cut(s) 185
TseFI GTSAC 5 cut(s) 105, 208, 677, 1051, 1378
TseI GCWGC 2 cut(s) 525, 528
Tsp45I GTSAC 5 cut(s) 105, 208, 677, 1051, 1378
TspDTI ATGAA 5 cut(s) 131, 462, 489, 581, 1224
TspGWI ACGGA 1 cut(s) 227
TspRI CASTG 1 cut(s) 185
Van91I CCANNNNNTGG 1 cut(s) 1397
Vha464I CTTAAG 1 cut(s) 1369
VpaK11BI GGWCC 3 cut(s) 23, 150, 1256
XapI RAATTY 2 cut(s) 479, 1263
XcmI CCANNNNNNNNNTGG 1 cut(s) 368
XspI CTAG 4 cut(s) 411, 428, 510, 1260
Zsp2I ATGCAT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.