Rorug05G0370200

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0031315
Physical Location & Seq
Forward (+)
49590188 .. 49590666
479 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0370200.1

Sequence Viewer

Length: 390 bp
ATGGGAACTAGTTCTCCTGGTACTGGTGGATCCATTGATCTCAATTTTGGGTCCACTGGTTCTACTGGTGCTGGTGGATCCATCGACATCAATTTTGGGCCAGCTGGTGGATCCATTGACATCAATTTTGGGTCCACTAGTTCTACTGATGATGGTAGATCCATTAATCTCAACTTTGGTTCCACCGATTCTACTGGAGGACAAACATCCCTGCAACAACGAGGAGGAGATCACCAGGAGATTGAAACCCTTCCATTGTTCCCCATGCCTGGTGAGGACATCTTTAGCAACATGAAGACTACTTCCGAGGGAGGTAGCGGTTATGGCGGTGGCTCTCGCATTTCCCTTGAGCTCAGTCTCAACTCCTCCGGAGCTGCTGATTTGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

129

Amino Acids

12.73

Weight (kDa)

4.05

Isoelectric Point (pI)

39.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031315)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0004720.1_g000002
rosa_rugosa Rorug05G0370200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 107
AccIII TCCGGA 1 cut(s) 368
AciI CCGC 2 cut(s) 318, 327
AclWI GGATC 7 cut(s) 24, 37, 72, 85, 105, 118, 153
AfaI GTAC 1 cut(s) 22
AfiI CCNNNNNNNGG 3 cut(s) 23, 107, 269
AgsI TTSAA 1 cut(s) 245
AhlI ACTAGT 2 cut(s) 8, 137
AjnI CCWGG 3 cut(s) 16, 234, 268
AloI GAACNNNNNNTCC 1 cut(s) 30
AluBI AGCT 3 cut(s) 104, 352, 374
AluI AGCT 3 cut(s) 104, 352, 374
Alw21I GWGCWC 1 cut(s) 354
Alw26I GTCTC 1 cut(s) 362
AlwI GGATC 7 cut(s) 24, 37, 72, 85, 105, 118, 153
Aor13HI TCCGGA 1 cut(s) 368
AoxI GGCC 1 cut(s) 98
ApeKI GCWGC 1 cut(s) 374
ArsI GACNNNNNNTTYG 4 cut(s) 77, 109, 110, 142
AseI ATTAAT 1 cut(s) 165
Asp700I GAANNNNTTC 2 cut(s) 10, 249
AspS9I GGNCC 3 cut(s) 51, 98, 132
AsuHPI GGTGA 2 cut(s) 224, 284
AvaII GGWCC 2 cut(s) 51, 132
BamHI GGATCC 3 cut(s) 29, 77, 110
BanII GRGCYC 1 cut(s) 354
BbsI GAAGAC 1 cut(s) 302
Bbv12I GWGCWC 1 cut(s) 354
BbvI GCAGC 1 cut(s) 361
BccI CCATC 2 cut(s) 89, 146
BciT130I CCWGG 3 cut(s) 18, 236, 270
BcoDI GTCTC 1 cut(s) 362
BcuI ACTAGT 2 cut(s) 8, 137
BfaI CTAG 2 cut(s) 9, 138
BisI GCNGC 1 cut(s) 375
BlsI GCNGC 1 cut(s) 376
Bme1390I CCNGG 3 cut(s) 18, 236, 270
Bme18I GGWCC 2 cut(s) 51, 132
BmgT120I GGNCC 3 cut(s) 51, 98, 132
BmiI GGNNCC 6 cut(s) 31, 52, 79, 112, 133, 181
BmrFI CCNGG 3 cut(s) 18, 236, 270
BpiI GAAGAC 1 cut(s) 302
BpmI CTGGAG 1 cut(s) 216
BpuEI CTTGAG 1 cut(s) 368
BsaJI CCNNGG 1 cut(s) 306
BsaWI WCCGGW 1 cut(s) 368
BsaXI ACNNNNNCTCC 1 cut(s) 28
Bsc4I CCNNNNNNNGG 3 cut(s) 23, 107, 269
Bse1I ACTGG 4 cut(s) 28, 61, 70, 199
BseAI TCCGGA 1 cut(s) 368
BseBI CCWGG 3 cut(s) 18, 236, 270
BseDI CCNNGG 1 cut(s) 306
BseGI GGATG 1 cut(s) 206
BseLI CCNNNNNNNGG 3 cut(s) 23, 107, 269
BseMII CTCAG 1 cut(s) 367
BseNI ACTGG 4 cut(s) 28, 61, 70, 199
BseRI GAGGAG 3 cut(s) 237, 240, 355
BseXI GCAGC 1 cut(s) 361
BshFI GGCC 1 cut(s) 100
BsiHKAI GWGCWC 1 cut(s) 354
BsiSI CCGG 1 cut(s) 369
BslI CCNNNNNNNGG 3 cut(s) 23, 107, 269
BsmAI GTCTC 1 cut(s) 362
BsnI GGCC 1 cut(s) 100
Bsp1286I GDGCHC 1 cut(s) 354
Bsp13I TCCGGA 1 cut(s) 368
Bsp143I GATC 6 cut(s) 29, 37, 77, 110, 158, 229
BspACI CCGC 2 cut(s) 318, 327
BspANI GGCC 1 cut(s) 100
BspCNI CTCAG 1 cut(s) 366
BspEI TCCGGA 1 cut(s) 368
BspLI GGNNCC 6 cut(s) 31, 52, 79, 112, 133, 181
BspPI GGATC 7 cut(s) 24, 37, 72, 85, 105, 118, 153
BsrI ACTGG 4 cut(s) 28, 61, 70, 199
BssECI CCNNGG 1 cut(s) 306
BssMI GATC 6 cut(s) 29, 37, 77, 110, 158, 229
Bst2UI CCWGG 3 cut(s) 18, 236, 270
BstC8I GCNNGC 1 cut(s) 102
BstDEI CTNAG 1 cut(s) 353
BstF5I GGATG 1 cut(s) 206
BstKTI GATC 6 cut(s) 32, 40, 80, 113, 161, 232
BstMAI GTCTC 1 cut(s) 362
BstMBI GATC 6 cut(s) 29, 37, 77, 110, 158, 229
BstMWI GCNNNNNNNGC 2 cut(s) 324, 383
BstNI CCWGG 3 cut(s) 18, 236, 270
BstSCI CCNGG 3 cut(s) 16, 234, 268
BstV1I GCAGC 1 cut(s) 361
BstV2I GAAGAC 1 cut(s) 302
BstX2I RGATCY 4 cut(s) 29, 77, 110, 158
BstYI RGATCY 4 cut(s) 29, 77, 110, 158
BsuRI GGCC 1 cut(s) 100
BtsCI GGATG 1 cut(s) 206
BtsIMutI CAGTG 1 cut(s) 54
Cac8I GCNNGC 1 cut(s) 102
Cfr13I GGNCC 3 cut(s) 51, 98, 132
Csp6I GTAC 1 cut(s) 21
CviAII CATG 2 cut(s) 265, 292
CviJI RGCY 5 cut(s) 100, 104, 333, 352, 374
CviKI_1 RGCY 5 cut(s) 100, 104, 333, 352, 374
CviQI GTAC 1 cut(s) 21
DdeI CTNAG 1 cut(s) 353
DpnI GATC 6 cut(s) 31, 39, 79, 112, 160, 231
DpnII GATC 6 cut(s) 29, 37, 77, 110, 158, 229
Ecl136II GAGCTC 1 cut(s) 352
Eco24I GRGCYC 1 cut(s) 354
Eco47I GGWCC 2 cut(s) 51, 132
Eco53kI GAGCTC 1 cut(s) 352
EcoICRI GAGCTC 1 cut(s) 352
EcoRII CCWGG 3 cut(s) 16, 234, 268
EcoT38I GRGCYC 1 cut(s) 354
FaeI CATG 2 cut(s) 268, 295
FaiI YATR 4 cut(s) 266, 293, 324, 388
FatI CATG 2 cut(s) 264, 291
Fnu4HI GCNGC 1 cut(s) 375
FokI GGATG 1 cut(s) 193
FriOI GRGCYC 1 cut(s) 354
Fsp4HI GCNGC 1 cut(s) 375
FspBI CTAG 2 cut(s) 9, 138
GluI GCNGC 1 cut(s) 375
GsuI CTGGAG 1 cut(s) 216
HaeIII GGCC 1 cut(s) 100
HapII CCGG 1 cut(s) 369
Hin1II CATG 2 cut(s) 268, 295
HinfI GANTC 1 cut(s) 188
HpaII CCGG 1 cut(s) 369
HphI GGTGA 2 cut(s) 224, 284
Hpy166II GTNNAC 2 cut(s) 54, 135
Hpy188I TCNGA 1 cut(s) 307
Hpy188III TCNNGA 1 cut(s) 369
Hpy8I GTNNAC 2 cut(s) 54, 135
HpyAV CCTTC 1 cut(s) 260
HpyCH4V TGCA 1 cut(s) 214
HpyF10VI GCNNNNNNNGC 2 cut(s) 324, 383
HpyF3I CTNAG 1 cut(s) 353
Hsp92II CATG 2 cut(s) 268, 295
Kpn2I TCCGGA 1 cut(s) 368
Kzo9I GATC 6 cut(s) 29, 37, 77, 110, 158, 229
LmnI GCTCC 1 cut(s) 371
Lsp1109I GCAGC 1 cut(s) 361
MaeI CTAG 2 cut(s) 9, 138
MalI GATC 6 cut(s) 31, 39, 79, 112, 160, 231
MboI GATC 6 cut(s) 29, 37, 77, 110, 158, 229
MboII GAAGA 1 cut(s) 307
MflI RGATCY 4 cut(s) 29, 77, 110, 158
MhlI GDGCHC 1 cut(s) 354
MluCI AATT 3 cut(s) 43, 91, 124
MnlI CCTC 7 cut(s) 191, 215, 218, 268, 301, 305, 376
MroI TCCGGA 1 cut(s) 368
MroXI GAANNNNTTC 2 cut(s) 10, 249
MseI TTAA 1 cut(s) 165
MspA1I CMGCKG 1 cut(s) 104
MspI CCGG 1 cut(s) 369
MspR9I CCNGG 3 cut(s) 18, 236, 270
MvaI CCWGG 3 cut(s) 18, 236, 270
MwoI GCNNNNNNNGC 2 cut(s) 324, 383
NdeII GATC 6 cut(s) 29, 37, 77, 110, 158, 229
NlaIII CATG 2 cut(s) 268, 295
NlaIV GGNNCC 6 cut(s) 31, 52, 79, 112, 133, 181
PdmI GAANNNNTTC 2 cut(s) 10, 249
PfeI GAWTC 1 cut(s) 188
PflMI CCANNNNNTGG 1 cut(s) 107
PkrI GCNGC 1 cut(s) 376
PshBI ATTAAT 1 cut(s) 165
Psp124BI GAGCTC 1 cut(s) 354
Psp6I CCWGG 3 cut(s) 16, 234, 268
PspGI CCWGG 3 cut(s) 16, 234, 268
PspN4I GGNNCC 6 cut(s) 31, 52, 79, 112, 133, 181
PspPI GGNCC 3 cut(s) 51, 98, 132
PsuI RGATCY 4 cut(s) 29, 77, 110, 158
PvuII CAGCTG 1 cut(s) 104
RsaI GTAC 1 cut(s) 22
RsaNI GTAC 1 cut(s) 21
SacI GAGCTC 1 cut(s) 354
SaqAI TTAA 1 cut(s) 165
SatI GCNGC 1 cut(s) 375
Sau3AI GATC 6 cut(s) 29, 37, 77, 110, 158, 229
Sau96I GGNCC 3 cut(s) 51, 98, 132
ScrFI CCNGG 3 cut(s) 18, 236, 270
SduI GDGCHC 1 cut(s) 354
SetI ASST 4 cut(s) 106, 316, 354, 376
SinI GGWCC 2 cut(s) 51, 132
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
SpeI ACTAGT 2 cut(s) 8, 137
Sse9I AATT 3 cut(s) 43, 91, 124
SsiI CCGC 2 cut(s) 318, 327
SspMI CTAG 2 cut(s) 9, 138
SstI GAGCTC 1 cut(s) 354
StyD4I CCNGG 3 cut(s) 16, 234, 268
TaqI TCGA 1 cut(s) 84
TasI AATT 3 cut(s) 43, 91, 124
TfiI GAWTC 1 cut(s) 188
Tru1I TTAA 1 cut(s) 165
Tru9I TTAA 1 cut(s) 165
TscAI CASTG 1 cut(s) 61
TseI GCWGC 1 cut(s) 374
TspDTI ATGAA 1 cut(s) 308
TspRI CASTG 1 cut(s) 61
Van91I CCANNNNNTGG 1 cut(s) 107
VpaK11BI GGWCC 2 cut(s) 51, 132
VspI ATTAAT 1 cut(s) 165
XmnI GAANNNNTTC 2 cut(s) 10, 249
XspI CTAG 2 cut(s) 9, 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.