Rorug06G0223200

Indole-3-acetic acid-amido synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010528
Physical Location & Seq
Forward (+)
35682443 .. 35683813
1371 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0223200.1

Sequence Viewer

Length: 474 bp
ATGGCGGGCGGGGACATTGTGGAGCTCCAAAGAAACTCTGCCAAGTGGGTAACAGTAGTGGAAGACATAGTGAAGCTAGAGAGGAAGATCTTTCCGAAACATGAATCGCTTGCTAGAAGCTTTGATGAAGAACTGAAAAAGAAGAACAGTGGGTTGCTTTACATCGAGTTAGATGGTTCAGTTGCTGGCTATGTCATGTACTCTTGGCCTTCTTCCCTGTACGCTTCTTTCACAAAGCTCGCAGTGAAGGATAGTTGCAGGAGGCAAGGCTATGGAGAGGCACTGCTGACAGCGGCCATTCAGAAATGCCGAACCCGAAACGTGGGGAGAATATCACTTCATGTTGATCCTTTGAGGACTCCTGCCATGAATCTTTACAAGAAACTCGGTTTCAAAGTTGATAACCTCATACAAGGGTACTACTCGTCGGATCGGAATGCCTACAGACTGTACTTGGATTTTGATGCCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
DUF295 GH3

Protein Analysis

157

Amino Acids

17.85

Weight (kDa)

9.28

Isoelectric Point (pI)

33.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acetyltransf_1 PF00583 23 - 131 2.5e-19 Acetyltransferase (GNAT) family
Acetyltransf_10 PF13673 31 - 134 1.4e-10 Acetyltransferase (GNAT) domain
Acetyltransf_7 PF13508 52 - 132 1.9e-12 Acetyltransferase (GNAT) domain
FR47 PF08445 76 - 134 2.7e-07 FR47-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 5, 9, 293
AclWI GGATC 2 cut(s) 341, 438
AcoI YGGCCR 1 cut(s) 294
AfaI GTAC 4 cut(s) 200, 221, 419, 452
AfiI CCNNNNNNNGG 1 cut(s) 322
AgsI TTSAA 1 cut(s) 394
AluBI AGCT 5 cut(s) 25, 76, 120, 238, 471
AluI AGCT 5 cut(s) 25, 76, 120, 238, 471
Alw21I GWGCWC 1 cut(s) 27
AlwI GGATC 2 cut(s) 341, 438
AlwNI CAGNNNCTG 1 cut(s) 185
AoxI GGCC 2 cut(s) 206, 294
BanII GRGCYC 1 cut(s) 27
BbsI GAAGAC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 27
BccI CCATC 1 cut(s) 167
BfaI CTAG 3 cut(s) 77, 114, 472
BfmI CTRYAG 1 cut(s) 442
BglII AGATCT 1 cut(s) 87
BisI GCNGC 1 cut(s) 294
BlsI GCNGC 1 cut(s) 295
BmsI GCATC 1 cut(s) 454
BpiI GAAGAC 1 cut(s) 69
Bsc4I CCNNNNNNNGG 1 cut(s) 322
BseLI CCNNNNNNNGG 1 cut(s) 322
BshFI GGCC 2 cut(s) 208, 296
BsiHKAI GWGCWC 1 cut(s) 27
BslFI GGGAC 1 cut(s) 26
BslI CCNNNNNNNGG 1 cut(s) 322
BsmFI GGGAC 1 cut(s) 26
BsmI GAATGC 1 cut(s) 442
BsnI GGCC 2 cut(s) 208, 296
Bsp1286I GDGCHC 1 cut(s) 27
Bsp143I GATC 3 cut(s) 87, 346, 430
BspACI CCGC 3 cut(s) 5, 9, 293
BspANI GGCC 2 cut(s) 208, 296
BspPI GGATC 2 cut(s) 341, 438
BssMI GATC 3 cut(s) 87, 346, 430
Bst4CI ACNGT 3 cut(s) 55, 149, 450
BstC8I GCNNGC 5 cut(s) 7, 111, 187, 240, 469
BstKTI GATC 3 cut(s) 90, 349, 433
BstMBI GATC 3 cut(s) 87, 346, 430
BstSFI CTRYAG 1 cut(s) 442
BstV2I GAAGAC 1 cut(s) 69
BstX2I RGATCY 1 cut(s) 87
BstYI RGATCY 1 cut(s) 87
BsuRI GGCC 2 cut(s) 208, 296
BtsI GCAGTG 2 cut(s) 249, 281
BtsIMutI CAGTG 3 cut(s) 154, 249, 281
Cac8I GCNNGC 5 cut(s) 7, 111, 187, 240, 469
CaiI CAGNNNCTG 1 cut(s) 185
Csp6I GTAC 4 cut(s) 199, 220, 418, 451
CviAII CATG 4 cut(s) 101, 196, 341, 367
CviJI RGCY 9 cut(s) 25, 76, 120, 189, 208, 238, 270, 296, 471
CviKI_1 RGCY 9 cut(s) 25, 76, 120, 189, 208, 238, 270, 296, 471
CviQI GTAC 4 cut(s) 199, 220, 418, 451
DpnI GATC 3 cut(s) 89, 348, 432
DpnII GATC 3 cut(s) 87, 346, 430
EaeI YGGCCR 1 cut(s) 294
Ecl136II GAGCTC 1 cut(s) 25
Eco24I GRGCYC 1 cut(s) 27
Eco53kI GAGCTC 1 cut(s) 25
EcoICRI GAGCTC 1 cut(s) 25
EcoT38I GRGCYC 1 cut(s) 27
FaeI CATG 4 cut(s) 104, 199, 344, 370
FaiI YATR 8 cut(s) 68, 102, 192, 197, 273, 342, 368, 410
FaqI GGGAC 1 cut(s) 26
FatI CATG 4 cut(s) 100, 195, 340, 366
FauI CCCGC 1 cut(s) 2
Fnu4HI GCNGC 1 cut(s) 294
FriOI GRGCYC 1 cut(s) 27
Fsp4HI GCNGC 1 cut(s) 294
FspBI CTAG 3 cut(s) 77, 114, 472
GluI GCNGC 1 cut(s) 294
HaeIII GGCC 2 cut(s) 208, 296
Hin1II CATG 4 cut(s) 104, 199, 344, 370
HindIII AAGCTT 1 cut(s) 118
HinfI GANTC 3 cut(s) 104, 358, 370
Hpy188I TCNGA 4 cut(s) 96, 303, 430, 435
Hpy99I CGWCG 1 cut(s) 430
HpyAV CCTTC 2 cut(s) 219, 241
HpyCH4III ACNGT 3 cut(s) 55, 149, 450
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 1 cut(s) 258
HpySE526I ACGT 1 cut(s) 321
Hsp92II CATG 4 cut(s) 104, 199, 344, 370
Kzo9I GATC 3 cut(s) 87, 346, 430
LmnI GCTCC 2 cut(s) 22, 30
LpnPI CCDG 4 cut(s) 171, 230, 244, 375
LweI GCATC 1 cut(s) 454
MaeI CTAG 3 cut(s) 77, 114, 472
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 1 cut(s) 49
MalI GATC 3 cut(s) 89, 348, 432
MboI GATC 3 cut(s) 87, 346, 430
MboII GAAGA 5 cut(s) 74, 97, 140, 154, 204
MflI RGATCY 1 cut(s) 87
MhlI GDGCHC 1 cut(s) 27
MlyI GAGTC 1 cut(s) 352
MmeI TCCRAC 1 cut(s) 408
MnlI CCTC 5 cut(s) 75, 255, 271, 348, 416
MspA1I CMGCKG 1 cut(s) 293
Mva1269I GAATGC 1 cut(s) 442
NdeII GATC 3 cut(s) 87, 346, 430
NlaIII CATG 4 cut(s) 104, 199, 344, 370
PctI GAATGC 1 cut(s) 442
PfeI GAWTC 2 cut(s) 104, 370
PkrI GCNGC 1 cut(s) 295
PleI GAGTC 1 cut(s) 352
PpsI GAGTC 1 cut(s) 352
Psp124BI GAGCTC 1 cut(s) 27
PstNI CAGNNNCTG 1 cut(s) 185
PsuI RGATCY 1 cut(s) 87
RsaI GTAC 4 cut(s) 200, 221, 419, 452
RsaNI GTAC 4 cut(s) 199, 220, 418, 451
SacI GAGCTC 1 cut(s) 27
SatI GCNGC 1 cut(s) 294
Sau3AI GATC 3 cut(s) 87, 346, 430
SchI GAGTC 1 cut(s) 352
SduI GDGCHC 1 cut(s) 27
SetI ASST 7 cut(s) 27, 78, 122, 240, 324, 408, 473
SfaNI GCATC 1 cut(s) 454
SfcI CTRYAG 1 cut(s) 442
SsiI CCGC 3 cut(s) 5, 9, 293
SspMI CTAG 3 cut(s) 77, 114, 472
SstI GAGCTC 1 cut(s) 27
TaaI ACNGT 3 cut(s) 55, 149, 450
TaiI ACGT 1 cut(s) 324
TaqI TCGA 1 cut(s) 165
TatI WGTACW 2 cut(s) 198, 450
TauI GCSGC 1 cut(s) 296
TfiI GAWTC 2 cut(s) 104, 370
TscAI CASTG 3 cut(s) 154, 249, 288
TspDTI ATGAA 4 cut(s) 117, 141, 329, 383
TspRI CASTG 3 cut(s) 154, 249, 288
XspI CTAG 3 cut(s) 77, 114, 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.