Rh1AG117300

DNA gyrase subunit A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0029632
Physical Location & Seq
Forward (+)
20145467 .. 20151366
5900 bp
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UTR
Exon/CDS
Intron
Rh1AG117300.1

Sequence Viewer

Length: 918 bp
ATGGGTATTGCTGTAGGCCTTGATAATCTGCAAAGAGTAATGCGTATAAGTTTGGAAGCTTCTAATAATGCAATTGCATCAACTCTTCTAAGAAATGAGTTCAACCTTTCTGAGACACAAGCTGAGGCAATATTAGACTTCAACTTCAGAAGGCTTAATGTTATGGAGAGGAAAAAGTTTGATAATGAAAGTGAATCGTTGAAAGAGCAAATTTCAAAATTAGAAGAACTATTCTCAAGCAAAAAACACATATTGCAGGTGGTTGAACAAGAAGCAATTGACATAAAAACCAAGTTTGCTAGTCACAGGCGTTCGATGCTGGAGGAAACTGATGGTGGTCAACTTGATGATATTGATGTTATTCCAAATGATGAAATGCTTTTGGCCTTCAGTGAAAAAGGGTATGTTAAGCGGATGAAGCCTAACACGTTTAATTTGCAAAATCGAGGTACCATTGGTAAATCTGTTGGGACATTGAGGGTAAACGATGCAATGTCAGACTTTATTGTTTGTCACGCACATGATCATGTCTTGTACTTCAGTGATAAAGGAACAGTCTACTCAGCTCGTGCATATAAAATTCCTGAGTGCAGCCGCACAGCAGCTGGTACACCACTGGTTCAGATCTTATCTCTGTCAGATGGTGAGAGAATTACATCTGTTATTCCTGTGAGTGAGTTTGCTGGAGATCAGTTTTTACTAATGCTCACAGTGAATGGCTACATAAAGAAGGTGTCTTTGAGTTCTTTTTCATCAATCAGGTCAACCGGAATCATAGCAATTCAATTGGTCCCTGGTGATGAGCTGAAATGGGTCCGTTGCTGCACAAATGATGACCTTGTAGCGATGGCATCCCTAAACGGAATGGTCATCTTATGTTCGTCTCATATTAAGGCAGGAACACTAGGGGTTCTATAG

Protein Analysis

305

Amino Acids

33.83

Weight (kDa)

5.92

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_topoisoIV PF00521 4 - 91 3.7e-07 DNA gyrase/topoisomerase IV, subunit A
DNA_gyraseA_C PF03989 125 - 171 1.5e-08 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 175 - 225 7.7e-08 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 232 - 275 8.8e-09 DNA gyrase C-terminal domain, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0029632)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0331301
rosa_samantha Rh1AG117300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 247
Acc36I ACCTGC 1 cut(s) 247
Acc65I GGTACC 1 cut(s) 449
AccB1I GGYRCC 1 cut(s) 449
AccI GTMKAC 1 cut(s) 558
AciI CCGC 2 cut(s) 412, 595
AcsI RAATTY 2 cut(s) 210, 579
AcuI CTGAAG 3 cut(s) 130, 373, 523
AfaI GTAC 3 cut(s) 451, 536, 610
AflIII ACRYGT 1 cut(s) 426
AgsI TTSAA 6 cut(s) 103, 142, 202, 216, 266, 785
AjnI CCWGG 1 cut(s) 793
AluBI AGCT 5 cut(s) 59, 122, 566, 605, 805
AluI AGCT 5 cut(s) 59, 122, 566, 605, 805
Alw26I GTCTC 2 cut(s) 107, 888
AlwNI CAGNNNCTG 1 cut(s) 605
AoxI GGCC 2 cut(s) 16, 384
ApeKI GCWGC 3 cut(s) 591, 602, 822
ApoI RAATTY 2 cut(s) 210, 579
Asp718I GGTACC 1 cut(s) 449
AspS9I GGNCC 2 cut(s) 790, 814
AsuHPI GGTGA 2 cut(s) 656, 809
AvaII GGWCC 2 cut(s) 790, 814
BanI GGYRCC 1 cut(s) 449
BauI CACGAG 1 cut(s) 567
BbvCI CCTCAGC 1 cut(s) 123
BbvI GCAGC 3 cut(s) 603, 614, 809
BccI CCATC 3 cut(s) 326, 635, 841
BciT130I CCWGG 1 cut(s) 795
BclI TGATCA 1 cut(s) 523
BcoDI GTCTC 2 cut(s) 107, 888
BfaI CTAG 2 cut(s) 300, 905
BfmI CTRYAG 2 cut(s) 12, 914
BfuAI ACCTGC 1 cut(s) 247
BglII AGATCT 1 cut(s) 624
BisI GCNGC 4 cut(s) 592, 595, 603, 823
BlsI GCNGC 4 cut(s) 593, 596, 604, 824
Bme1390I CCNGG 1 cut(s) 795
Bme18I GGWCC 2 cut(s) 790, 814
BmgT120I GGNCC 2 cut(s) 790, 814
BmiI GGNNCC 3 cut(s) 451, 792, 815
BmrFI CCNGG 1 cut(s) 795
BmsI GCATC 4 cut(s) 86, 306, 478, 860
BpmI CTGGAG 2 cut(s) 341, 705
Bpu10I CCTNAGC 1 cut(s) 123
BpuEI CTTGAG 1 cut(s) 220
BsaJI CCNNGG 1 cut(s) 793
BsaWI WCCGGW 1 cut(s) 767
Bse1I ACTGG 1 cut(s) 621
Bse3DI GCAATG 1 cut(s) 498
BseBI CCWGG 1 cut(s) 795
BseDI CCNNGG 1 cut(s) 793
BseGI GGATG 2 cut(s) 420, 851
BseMI GCAATG 1 cut(s) 498
BseMII CTCAG 4 cut(s) 102, 114, 576, 576
BseNI ACTGG 1 cut(s) 621
BseXI GCAGC 3 cut(s) 603, 614, 809
BsgI GTGCAG 2 cut(s) 610, 808
BshFI GGCC 2 cut(s) 18, 386
BshNI GGYRCC 1 cut(s) 449
BsiSI CCGG 1 cut(s) 768
BslFI GGGAC 2 cut(s) 484, 776
BsmAI GTCTC 2 cut(s) 107, 888
BsmBI CGTCTC 1 cut(s) 888
BsmFI GGGAC 2 cut(s) 484, 776
BsnI GGCC 2 cut(s) 18, 386
Bsp143I GATC 3 cut(s) 523, 624, 688
BspACI CCGC 2 cut(s) 412, 595
BspANI GGCC 2 cut(s) 18, 386
BspCNI CTCAG 4 cut(s) 103, 115, 575, 577
BspLI GGNNCC 3 cut(s) 451, 792, 815
BspMI ACCTGC 1 cut(s) 247
BspT107I GGYRCC 1 cut(s) 449
BsrDI GCAATG 1 cut(s) 498
BsrI ACTGG 1 cut(s) 621
BssECI CCNNGG 1 cut(s) 793
BssMI GATC 3 cut(s) 523, 624, 688
BssSI CACGAG 1 cut(s) 567
Bst2BI CACGAG 1 cut(s) 567
Bst2UI CCWGG 1 cut(s) 795
Bst4CI ACNGT 2 cut(s) 556, 712
Bst6I CTCTTC 1 cut(s) 90
BstDEI CTNAG 5 cut(s) 89, 111, 123, 562, 585
BstF5I GGATG 2 cut(s) 420, 851
BstKTI GATC 3 cut(s) 526, 627, 691
BstMAI GTCTC 2 cut(s) 107, 888
BstMBI GATC 3 cut(s) 523, 624, 688
BstMWI GCNNNNNNNGC 2 cut(s) 316, 418
BstNI CCWGG 1 cut(s) 795
BstSCI CCNGG 1 cut(s) 793
BstSFI CTRYAG 2 cut(s) 12, 914
BstV1I GCAGC 3 cut(s) 603, 614, 809
BstX2I RGATCY 1 cut(s) 624
BstYI RGATCY 1 cut(s) 624
BsuRI GGCC 2 cut(s) 18, 386
BtgZI GCGATG 1 cut(s) 860
BtsCI GGATG 2 cut(s) 420, 851
BtsIMutI CAGTG 4 cut(s) 397, 547, 614, 717
BveI ACCTGC 1 cut(s) 247
CaiI CAGNNNCTG 1 cut(s) 605
Cfr13I GGNCC 2 cut(s) 790, 814
Csp6I GTAC 3 cut(s) 450, 535, 609
CviAII CATG 2 cut(s) 521, 527
CviQI GTAC 3 cut(s) 450, 535, 609
DdeI CTNAG 5 cut(s) 89, 111, 123, 562, 585
DpnI GATC 3 cut(s) 525, 626, 690
DpnII GATC 3 cut(s) 523, 624, 688
Eam1104I CTCTTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 90
Eco147I AGGCCT 1 cut(s) 18
Eco47I GGWCC 2 cut(s) 790, 814
Eco57I CTGAAG 3 cut(s) 130, 373, 523
EcoRII CCWGG 1 cut(s) 793
Esp3I CGTCTC 1 cut(s) 888
FaeI CATG 2 cut(s) 524, 530
FaqI GGGAC 2 cut(s) 484, 776
FatI CATG 2 cut(s) 520, 526
FbaI TGATCA 1 cut(s) 523
FblI GTMKAC 1 cut(s) 558
Fnu4HI GCNGC 4 cut(s) 592, 595, 603, 823
FokI GGATG 2 cut(s) 427, 838
Fsp4HI GCNGC 4 cut(s) 592, 595, 603, 823
FspBI CTAG 2 cut(s) 300, 905
GluI GCNGC 4 cut(s) 592, 595, 603, 823
GsuI CTGGAG 2 cut(s) 341, 705
HaeIII GGCC 2 cut(s) 18, 386
HapII CCGG 1 cut(s) 768
Hin1II CATG 2 cut(s) 524, 530
HincII GTYRAC 2 cut(s) 341, 765
HindII GTYRAC 2 cut(s) 341, 765
HindIII AAGCTT 1 cut(s) 57
HinfI GANTC 2 cut(s) 194, 771
HpaII CCGG 1 cut(s) 768
HphI GGTGA 2 cut(s) 656, 809
Hpy166II GTNNAC 5 cut(s) 341, 484, 559, 611, 765
Hpy188I TCNGA 5 cut(s) 112, 149, 499, 624, 640
Hpy188III TCNNGA 1 cut(s) 584
Hpy8I GTNNAC 5 cut(s) 341, 484, 559, 611, 765
HpyAV CCTTC 3 cut(s) 144, 397, 724
HpyCH4III ACNGT 2 cut(s) 556, 712
HpyCH4IV ACGT 1 cut(s) 428
HpyCH4V TGCA 9 cut(s) 31, 71, 77, 256, 439, 491, 572, 591, 825
HpyF10VI GCNNNNNNNGC 2 cut(s) 316, 418
HpyF3I CTNAG 5 cut(s) 89, 111, 123, 562, 585
HpySE526I ACGT 1 cut(s) 428
Hsp92II CATG 2 cut(s) 524, 530
KpnI GGTACC 1 cut(s) 453
Ksp22I TGATCA 1 cut(s) 523
Kzo9I GATC 3 cut(s) 523, 624, 688
Lsp1109I GCAGC 3 cut(s) 603, 614, 809
LweI GCATC 4 cut(s) 86, 306, 478, 860
MaeI CTAG 2 cut(s) 300, 905
MaeII ACGT 1 cut(s) 428
MaeIII GTNAC 2 cut(s) 302, 512
MalI GATC 3 cut(s) 525, 626, 690
MboI GATC 3 cut(s) 523, 624, 688
MboII GAAGA 2 cut(s) 77, 236
MfeI CAATTG 3 cut(s) 72, 276, 785
MflI RGATCY 1 cut(s) 624
MluCI AATT 9 cut(s) 72, 210, 218, 276, 433, 579, 651, 780, 785
MnlI CCTC 5 cut(s) 118, 162, 316, 440, 471
MseI TTAA 4 cut(s) 156, 408, 432, 891
MslI CAYNNNNRTG 2 cut(s) 519, 525
MspA1I CMGCKG 1 cut(s) 605
MspI CCGG 1 cut(s) 768
MspR9I CCNGG 1 cut(s) 795
MunI CAATTG 3 cut(s) 72, 276, 785
MvaI CCWGG 1 cut(s) 795
MwoI GCNNNNNNNGC 2 cut(s) 316, 418
NdeII GATC 3 cut(s) 523, 624, 688
NlaIII CATG 2 cut(s) 524, 530
NlaIV GGNNCC 3 cut(s) 451, 792, 815
NmuCI GTSAC 2 cut(s) 302, 512
PaqCI CACCTGC 1 cut(s) 247
PceI AGGCCT 1 cut(s) 18
PfeI GAWTC 2 cut(s) 194, 771
PkrI GCNGC 4 cut(s) 593, 596, 604, 824
Psp6I CCWGG 1 cut(s) 793
PspGI CCWGG 1 cut(s) 793
PspN4I GGNNCC 3 cut(s) 451, 792, 815
PspPI GGNCC 2 cut(s) 790, 814
PstNI CAGNNNCTG 1 cut(s) 605
PsuI RGATCY 1 cut(s) 624
PvuII CAGCTG 1 cut(s) 605
RsaI GTAC 3 cut(s) 451, 536, 610
RsaNI GTAC 3 cut(s) 450, 535, 609
RseI CAYNNNNRTG 2 cut(s) 519, 525
SaqAI TTAA 4 cut(s) 156, 408, 432, 891
SatI GCNGC 4 cut(s) 592, 595, 603, 823
Sau3AI GATC 3 cut(s) 523, 624, 688
Sau96I GGNCC 2 cut(s) 790, 814
ScrFI CCNGG 1 cut(s) 795
SfaNI GCATC 4 cut(s) 86, 306, 478, 860
SfcI CTRYAG 2 cut(s) 12, 914
SinI GGWCC 2 cut(s) 790, 814
SmiMI CAYNNNNRTG 2 cut(s) 519, 525
SmlI CTYRAG 1 cut(s) 235
SmoI CTYRAG 1 cut(s) 235
Sse9I AATT 9 cut(s) 72, 210, 218, 276, 433, 579, 651, 780, 785
SseBI AGGCCT 1 cut(s) 18
SsiI CCGC 2 cut(s) 412, 595
SspI AATATT 1 cut(s) 132
SspMI CTAG 2 cut(s) 300, 905
StuI AGGCCT 1 cut(s) 18
StyD4I CCNGG 1 cut(s) 793
TaaI ACNGT 2 cut(s) 556, 712
TaiI ACGT 1 cut(s) 431
TaqI TCGA 2 cut(s) 314, 445
TasI AATT 9 cut(s) 72, 210, 218, 276, 433, 579, 651, 780, 785
TatI WGTACW 1 cut(s) 534
TauI GCSGC 1 cut(s) 597
TfiI GAWTC 2 cut(s) 194, 771
Tru1I TTAA 4 cut(s) 156, 408, 432, 891
Tru9I TTAA 4 cut(s) 156, 408, 432, 891
TscAI CASTG 4 cut(s) 397, 547, 621, 717
TseFI GTSAC 2 cut(s) 302, 512
TseI GCWGC 3 cut(s) 591, 602, 822
Tsp45I GTSAC 2 cut(s) 302, 512
TspDTI ATGAA 4 cut(s) 201, 387, 431, 741
TspGWI ACGGA 2 cut(s) 806, 876
TspRI CASTG 4 cut(s) 397, 547, 621, 717
VpaK11BI GGWCC 2 cut(s) 790, 814
XapI RAATTY 2 cut(s) 210, 579
XmiI GTMKAC 1 cut(s) 558
XspI CTAG 2 cut(s) 300, 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.