Rh1BG233000
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0026194
Physical Location & Seq
Forward (+)
35580348 .. 35581485
1138 bp
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UTR
Exon/CDS
Intron
Rh1BG233000.1

Sequence Viewer

Length: 531 bp
ATGCTGTCGCAGGTGGAGCATTTGAGCCTGGTCAAGTTTTATGGGTATGTGGAGTATGAAGATGAGAAAGTTGTGGTTGTGGAGTATGTTCCTAATGGAACCCTCAGAGAACATTTGGATTGTAAGTTGTATTACTGGGTTTTTTCGCGCTTTTGGTTCCCGAATTTTGCTTTTAAGTGTCTGAATGTCATAGAAATGCATAATGCAGGTCTGCACGGCAACATTCTTGACCTTGCGGCGCGGCTTGACATTGCAATCGATGTGGCTCATGCTGCTATGAAGAAGTTTAGTGATGGAGATGCACTTTTAATTTTGGATCCCAGGCTGGAAAAGAGTGCAGCCAATAATTTAGCCATTGAAAAGATTCTTGAACTATCCTTGCAATGCTTGGCTCAACGTAGACAGCACCGGCCTAGCATGAAGAGGTGCGCGGAGATCCTCTGGAGCATCCGAAAGGACTATAAAGAGATATCAGTTCCAGACTTTCGTTCATCTTCCACCCGTTCGCAAAGGAGCACTTTAACAGAGTAA

Protein Analysis

176

Amino Acids

20.41

Weight (kDa)

7.01

Isoelectric Point (pI)

52.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 1 - 91 7.1e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0026194)

Species Orthologous Gene IDs
rosa_samantha Rh1AG265500 Rh1BG233000 Rh1DG260500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 197
AccI GTMKAC 1 cut(s) 400
AccII CGCG 3 cut(s) 148, 241, 431
AciI CCGC 3 cut(s) 236, 241, 431
AclWI GGATC 3 cut(s) 311, 324, 430
AcsI RAATTY 1 cut(s) 163
AgsI TTSAA 2 cut(s) 359, 371
AjnI CCWGG 2 cut(s) 27, 320
Alw21I GWGCWC 1 cut(s) 518
AlwI GGATC 3 cut(s) 311, 324, 430
AoxI GGCC 1 cut(s) 410
ApeKI GCWGC 2 cut(s) 272, 338
ApoI RAATTY 1 cut(s) 163
Asp700I GAANNNNTTC 1 cut(s) 363
AspLEI GCGC 3 cut(s) 150, 241, 431
BamHI GGATCC 1 cut(s) 316
Bbv12I GWGCWC 1 cut(s) 518
BbvI GCAGC 2 cut(s) 259, 350
BccI CCATC 1 cut(s) 287
BceAI ACGGC 1 cut(s) 232
BciT130I CCWGG 2 cut(s) 29, 322
BfaI CTAG 1 cut(s) 414
BfuAI ACCTGC 1 cut(s) 197
BisI GCNGC 4 cut(s) 237, 242, 273, 339
BlsI GCNGC 4 cut(s) 238, 243, 274, 340
Bme1390I CCNGG 2 cut(s) 29, 322
BmiI GGNNCC 3 cut(s) 100, 158, 318
BmrFI CCNGG 2 cut(s) 29, 322
BmrI ACTGGG 1 cut(s) 145
BmsI GCATC 2 cut(s) 289, 456
BmuI ACTGGG 1 cut(s) 145
BpmI CTGGAG 1 cut(s) 463
Bsa29I ATCGAT 1 cut(s) 258
BsaJI CCNNGG 1 cut(s) 320
Bse118I RCCGGY 1 cut(s) 408
Bse1I ACTGG 1 cut(s) 140
Bse3DI GCAATG 2 cut(s) 249, 389
BseBI CCWGG 2 cut(s) 29, 322
BseCI ATCGAT 1 cut(s) 258
BseDI CCNNGG 1 cut(s) 320
BseGI GGATG 1 cut(s) 447
BseMI GCAATG 2 cut(s) 249, 389
BseMII CTCAG 1 cut(s) 118
BseNI ACTGG 1 cut(s) 140
BseXI GCAGC 2 cut(s) 259, 350
BsgI GTGCAG 2 cut(s) 197, 357
Bsh1236I CGCG 3 cut(s) 148, 241, 431
BshFI GGCC 1 cut(s) 412
BshVI ATCGAT 1 cut(s) 258
BsiHKAI GWGCWC 1 cut(s) 518
BsiSI CCGG 1 cut(s) 409
BsnI GGCC 1 cut(s) 412
Bsp1286I GDGCHC 1 cut(s) 518
Bsp143I GATC 2 cut(s) 316, 435
BspACI CCGC 3 cut(s) 236, 241, 431
BspANI GGCC 1 cut(s) 412
BspCNI CTCAG 1 cut(s) 117
BspDI ATCGAT 1 cut(s) 258
BspFNI CGCG 3 cut(s) 148, 241, 431
BspLI GGNNCC 3 cut(s) 100, 158, 318
BspMI ACCTGC 1 cut(s) 197
BspPI GGATC 3 cut(s) 311, 324, 430
BsrDI GCAATG 2 cut(s) 249, 389
BsrFI RCCGGY 1 cut(s) 408
BsrI ACTGG 1 cut(s) 140
BssAI RCCGGY 1 cut(s) 408
BssECI CCNNGG 1 cut(s) 320
BssMI GATC 2 cut(s) 316, 435
Bst2UI CCWGG 2 cut(s) 29, 322
Bst6I CTCTTC 1 cut(s) 416
BstDEI CTNAG 1 cut(s) 104
BstF5I GGATG 1 cut(s) 447
BstFNI CGCG 3 cut(s) 148, 241, 431
BstHHI GCGC 3 cut(s) 150, 241, 431
BstKTI GATC 2 cut(s) 319, 438
BstMBI GATC 2 cut(s) 316, 435
BstMWI GCNNNNNNNGC 2 cut(s) 16, 272
BstNI CCWGG 2 cut(s) 29, 322
BstSCI CCNGG 2 cut(s) 27, 320
BstUI CGCG 3 cut(s) 148, 241, 431
BstV1I GCAGC 2 cut(s) 259, 350
BstX2I RGATCY 2 cut(s) 316, 435
BstYI RGATCY 2 cut(s) 316, 435
Bsu15I ATCGAT 1 cut(s) 258
BsuRI GGCC 1 cut(s) 412
BsuTUI ATCGAT 1 cut(s) 258
BtsCI GGATG 1 cut(s) 447
BveI ACCTGC 1 cut(s) 197
CfoI GCGC 3 cut(s) 150, 241, 431
Cfr10I RCCGGY 1 cut(s) 408
ClaI ATCGAT 1 cut(s) 258
CspCI CAANNNNNGTGG 2 cut(s) 243, 278
CviAII CATG 2 cut(s) 269, 418
CviJI RGCY 8 cut(s) 27, 244, 266, 325, 341, 353, 392, 412
CviKI_1 RGCY 8 cut(s) 27, 244, 266, 325, 341, 353, 392, 412
DdeI CTNAG 1 cut(s) 104
DpnI GATC 2 cut(s) 318, 437
DpnII GATC 2 cut(s) 316, 435
Eam1104I CTCTTC 1 cut(s) 416
EarI CTCTTC 1 cut(s) 416
Eco32I GATATC 1 cut(s) 471
EcoRII CCWGG 2 cut(s) 27, 320
EcoRV GATATC 1 cut(s) 471
EcoT22I ATGCAT 1 cut(s) 201
FaeI CATG 2 cut(s) 272, 421
FalI AAGNNNNNCTT 1 cut(s) 502
FatI CATG 2 cut(s) 268, 417
FblI GTMKAC 1 cut(s) 400
Fnu4HI GCNGC 4 cut(s) 237, 242, 273, 339
FokI GGATG 1 cut(s) 434
Fsp4HI GCNGC 4 cut(s) 237, 242, 273, 339
FspBI CTAG 1 cut(s) 414
GlaI GCGC 3 cut(s) 149, 240, 430
GluI GCNGC 4 cut(s) 237, 242, 273, 339
GsuI CTGGAG 1 cut(s) 463
HaeIII GGCC 1 cut(s) 412
HapII CCGG 1 cut(s) 409
HhaI GCGC 3 cut(s) 150, 241, 431
Hin1II CATG 2 cut(s) 272, 421
Hin6I GCGC 3 cut(s) 148, 239, 429
HinP1I GCGC 3 cut(s) 148, 239, 429
HinfI GANTC 1 cut(s) 364
HpaII CCGG 1 cut(s) 409
Hpy166II GTNNAC 1 cut(s) 401
Hpy188I TCNGA 3 cut(s) 107, 183, 452
Hpy188III TCNNGA 5 cut(s) 160, 227, 368, 442, 479
Hpy8I GTNNAC 1 cut(s) 401
HpyCH4IV ACGT 1 cut(s) 397
HpyCH4V TGCA 7 cut(s) 199, 206, 214, 254, 302, 338, 382
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 272
HpyF3I CTNAG 1 cut(s) 104
HpySE526I ACGT 1 cut(s) 397
Hsp92II CATG 2 cut(s) 272, 421
HspAI GCGC 3 cut(s) 148, 239, 429
Kzo9I GATC 2 cut(s) 316, 435
LmnI GCTCC 3 cut(s) 16, 444, 513
Lsp1109I GCAGC 2 cut(s) 259, 350
LweI GCATC 2 cut(s) 289, 456
MaeI CTAG 1 cut(s) 414
MaeII ACGT 1 cut(s) 397
MalI GATC 2 cut(s) 318, 437
MboI GATC 2 cut(s) 316, 435
MboII GAAGA 4 cut(s) 71, 292, 433, 486
MflI RGATCY 2 cut(s) 316, 435
MhlI GDGCHC 1 cut(s) 518
MluCI AATT 3 cut(s) 163, 309, 346
MnlI CCTC 3 cut(s) 113, 417, 449
Mph1103I ATGCAT 1 cut(s) 201
MroXI GAANNNNTTC 1 cut(s) 363
MseI TTAA 3 cut(s) 174, 308, 521
MslI CAYNNNNRTG 1 cut(s) 194
MspI CCGG 1 cut(s) 409
MspR9I CCNGG 2 cut(s) 29, 322
MvaI CCWGG 2 cut(s) 29, 322
MvnI CGCG 3 cut(s) 148, 241, 431
MwoI GCNNNNNNNGC 2 cut(s) 16, 272
NdeII GATC 2 cut(s) 316, 435
NlaIII CATG 2 cut(s) 272, 421
NlaIV GGNNCC 3 cut(s) 100, 158, 318
NsiI ATGCAT 1 cut(s) 201
PdmI GAANNNNTTC 1 cut(s) 363
PfeI GAWTC 1 cut(s) 364
PkrI GCNGC 4 cut(s) 238, 243, 274, 340
Psp6I CCWGG 2 cut(s) 27, 320
PspGI CCWGG 2 cut(s) 27, 320
PspN4I GGNNCC 3 cut(s) 100, 158, 318
PsuI RGATCY 2 cut(s) 316, 435
RseI CAYNNNNRTG 1 cut(s) 194
SaqAI TTAA 3 cut(s) 174, 308, 521
SatI GCNGC 4 cut(s) 237, 242, 273, 339
Sau3AI GATC 2 cut(s) 316, 435
ScrFI CCNGG 2 cut(s) 29, 322
SduI GDGCHC 1 cut(s) 518
SetI ASST 5 cut(s) 15, 211, 234, 400, 428
SfaNI GCATC 2 cut(s) 289, 456
SmiMI CAYNNNNRTG 1 cut(s) 194
Sse9I AATT 3 cut(s) 163, 309, 346
SsiI CCGC 3 cut(s) 236, 241, 431
SspMI CTAG 1 cut(s) 414
StyD4I CCNGG 2 cut(s) 27, 320
TaiI ACGT 1 cut(s) 400
TaqI TCGA 1 cut(s) 258
TasI AATT 3 cut(s) 163, 309, 346
TauI GCSGC 2 cut(s) 239, 244
TfiI GAWTC 1 cut(s) 364
Tru1I TTAA 3 cut(s) 174, 308, 521
Tru9I TTAA 3 cut(s) 174, 308, 521
TseI GCWGC 2 cut(s) 272, 338
TspDTI ATGAA 4 cut(s) 72, 293, 434, 480
XapI RAATTY 1 cut(s) 163
XmiI GTMKAC 1 cut(s) 400
XmnI GAANNNNTTC 1 cut(s) 363
XspI CTAG 1 cut(s) 414
Zsp2I ATGCAT 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.