Rh1BG271400

omega-3 fatty acid desaturase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0023107
Physical Location & Seq
Forward (+)
40681995 .. 40682417
423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG271400.1

Sequence Viewer

Length: 423 bp
ATGGCTCTTCTTCGTCTTCATCTTCTGGAAATTTTCAGTTTGGTTTTTCTGTTTCGGCCTCACCCCCAGATTTCACCGAGACCCAGAATCAGAATCGCTACAGACCCAATAAAGCTTCCAACTTTGGCGAAGAACAACATCACAAATCTCGGATTCGGGTCGACCCGATTTTCAAGTGGGCGCTTTAGAAGTTGGGGCGTGAAGGTGAAAGTTGCTTATACAGAAGACAGGGAAGAGAGAATGAGAGTCAATGACGTTAATGGAGATGAAGACGAGATTGAATTTGATCCGGTTTCCCATCCACCTTTCAAATTGGCATATGCTAAAGCAGCAACCCCAAAGCATTGTTGGGTTGAGGACCTATGGAGGTCCATGAGTTATGTGGTTAGGGATGTGTTGATGGTTTTGGGCACTGGAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3474 FA_desaturase

Protein Analysis

140

Amino Acids

16.11

Weight (kDa)

9.2

Isoelectric Point (pI)

46.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3474 PF11960 18 - 137 4.5e-28 Domain of unknown function (DUF3474)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0023107)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0004593.1_g000009
rosa_samantha Rh1BG271400 Rh1DG301700
rosa_wichuraiana Rw4G030370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 161
AclWI GGATC 1 cut(s) 281
AcsI RAATTY 2 cut(s) 30, 281
AgsI TTSAA 3 cut(s) 174, 281, 310
AluBI AGCT 1 cut(s) 115
AluI AGCT 1 cut(s) 115
Alw26I GTCTC 1 cut(s) 73
AlwI GGATC 1 cut(s) 281
AoxI GGCC 1 cut(s) 56
ApeKI GCWGC 1 cut(s) 329
ApoI RAATTY 2 cut(s) 30, 281
AspLEI GCGC 1 cut(s) 183
AspS9I GGNCC 2 cut(s) 358, 369
AsuHPI GGTGA 3 cut(s) 53, 66, 217
AvaII GGWCC 2 cut(s) 358, 369
BaeGI GKGCMC 1 cut(s) 413
BbsI GAAGAC 3 cut(s) 8, 231, 276
BbvI GCAGC 1 cut(s) 341
BccI CCATC 2 cut(s) 306, 394
BcoDI GTCTC 1 cut(s) 73
BfmI CTRYAG 1 cut(s) 99
BfoI RGCGCY 1 cut(s) 184
BisI GCNGC 1 cut(s) 330
BlsI GCNGC 1 cut(s) 331
Bme18I GGWCC 2 cut(s) 358, 369
BmgT120I GGNCC 2 cut(s) 358, 369
BpiI GAAGAC 3 cut(s) 8, 231, 276
BsaI GGTCTC 1 cut(s) 73
BsaWI WCCGGW 1 cut(s) 289
Bse1I ACTGG 1 cut(s) 418
BseGI GGATG 2 cut(s) 298, 397
BseNI ACTGG 1 cut(s) 418
BseSI GKGCMC 1 cut(s) 413
BseXI GCAGC 1 cut(s) 341
BshFI GGCC 1 cut(s) 58
BsiSI CCGG 1 cut(s) 290
BsmAI GTCTC 1 cut(s) 73
BsnI GGCC 1 cut(s) 58
Bso31I GGTCTC 1 cut(s) 73
Bsp1286I GDGCHC 1 cut(s) 413
Bsp143I GATC 1 cut(s) 286
BspANI GGCC 1 cut(s) 58
BspPI GGATC 1 cut(s) 281
BspQI GCTCTTC 1 cut(s) 12
BspTNI GGTCTC 1 cut(s) 73
BsrI ACTGG 1 cut(s) 418
BssMI GATC 1 cut(s) 286
Bst6I CTCTTC 2 cut(s) 12, 228
BstF5I GGATG 2 cut(s) 298, 397
BstH2I RGCGCY 1 cut(s) 184
BstHHI GCGC 1 cut(s) 183
BstKTI GATC 1 cut(s) 289
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 1 cut(s) 286
BstMWI GCNNNNNNNGC 1 cut(s) 329
BstSFI CTRYAG 1 cut(s) 99
BstSLI GKGCMC 1 cut(s) 413
BstV1I GCAGC 1 cut(s) 341
BstV2I GAAGAC 3 cut(s) 8, 231, 276
BsuRI GGCC 1 cut(s) 58
BtsCI GGATG 2 cut(s) 298, 397
BtsIMutI CAGTG 1 cut(s) 411
CfoI GCGC 1 cut(s) 183
Cfr13I GGNCC 2 cut(s) 358, 369
CviAII CATG 1 cut(s) 373
CviJI RGCY 3 cut(s) 5, 58, 115
CviKI_1 RGCY 3 cut(s) 5, 58, 115
DpnI GATC 1 cut(s) 288
DpnII GATC 1 cut(s) 286
Eam1104I CTCTTC 2 cut(s) 12, 228
EarI CTCTTC 2 cut(s) 12, 228
Eco31I GGTCTC 1 cut(s) 73
Eco47I GGWCC 2 cut(s) 358, 369
EcoO109I RGGNCCY 1 cut(s) 358
FaeI CATG 1 cut(s) 376
FaiI YATR 6 cut(s) 219, 319, 321, 364, 374, 381
FatI CATG 1 cut(s) 372
FauNDI CATATG 1 cut(s) 319
FblI GTMKAC 1 cut(s) 161
Fnu4HI GCNGC 1 cut(s) 330
FokI GGATG 2 cut(s) 285, 404
Fsp4HI GCNGC 1 cut(s) 330
GlaI GCGC 1 cut(s) 182
GluI GCNGC 1 cut(s) 330
HaeII RGCGCY 1 cut(s) 184
HaeIII GGCC 1 cut(s) 58
HapII CCGG 1 cut(s) 290
HhaI GCGC 1 cut(s) 183
Hin1II CATG 1 cut(s) 376
Hin6I GCGC 1 cut(s) 181
HinP1I GCGC 1 cut(s) 181
HincII GTYRAC 1 cut(s) 162
HindII GTYRAC 1 cut(s) 162
HindIII AAGCTT 1 cut(s) 113
HinfI GANTC 4 cut(s) 87, 93, 153, 246
HpaII CCGG 1 cut(s) 290
HphI GGTGA 3 cut(s) 53, 66, 217
Hpy166II GTNNAC 1 cut(s) 162
Hpy188I TCNGA 2 cut(s) 92, 152
Hpy188III TCNNGA 1 cut(s) 26
Hpy8I GTNNAC 1 cut(s) 162
HpyAV CCTTC 1 cut(s) 196
HpyCH4IV ACGT 1 cut(s) 255
HpyF10VI GCNNNNNNNGC 1 cut(s) 329
HpySE526I ACGT 1 cut(s) 255
Hsp92II CATG 1 cut(s) 376
HspAI GCGC 1 cut(s) 181
Kzo9I GATC 1 cut(s) 286
LguI GCTCTTC 1 cut(s) 12
LpnPI CCDG 6 cut(s) 11, 80, 97, 214, 303, 399
Lsp1109I GCAGC 1 cut(s) 341
MaeII ACGT 1 cut(s) 255
MalI GATC 1 cut(s) 288
MboI GATC 1 cut(s) 286
MboII GAAGA 6 cut(s) 8, 14, 142, 236, 245, 281
MhlI GDGCHC 1 cut(s) 413
MluCI AATT 3 cut(s) 30, 281, 311
MlyI GAGTC 1 cut(s) 255
MmeI TCCRAC 1 cut(s) 143
MnlI CCTC 3 cut(s) 69, 349, 360
MseI TTAA 1 cut(s) 258
MspI CCGG 1 cut(s) 290
MwoI GCNNNNNNNGC 1 cut(s) 329
NdeI CATATG 1 cut(s) 319
NdeII GATC 1 cut(s) 286
NlaIII CATG 1 cut(s) 376
PciSI GCTCTTC 1 cut(s) 12
PfeI GAWTC 3 cut(s) 87, 93, 153
PkrI GCNGC 1 cut(s) 331
PleI GAGTC 1 cut(s) 254
PpsI GAGTC 1 cut(s) 254
PpuMI RGGWCCY 1 cut(s) 358
Psp5II RGGWCCY 1 cut(s) 358
PspPI GGNCC 2 cut(s) 358, 369
PspPPI RGGWCCY 1 cut(s) 358
SalI GTCGAC 1 cut(s) 160
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 1 cut(s) 330
Sau3AI GATC 1 cut(s) 286
Sau96I GGNCC 2 cut(s) 358, 369
SchI GAGTC 1 cut(s) 255
SduI GDGCHC 1 cut(s) 413
SetI ASST 6 cut(s) 117, 207, 258, 307, 363, 371
SfcI CTRYAG 1 cut(s) 99
SinI GGWCC 2 cut(s) 358, 369
Sse9I AATT 3 cut(s) 30, 281, 311
TaiI ACGT 1 cut(s) 258
TaqI TCGA 1 cut(s) 161
TasI AATT 3 cut(s) 30, 281, 311
TfiI GAWTC 3 cut(s) 87, 93, 153
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TscAI CASTG 1 cut(s) 418
TseI GCWGC 1 cut(s) 329
TspDTI ATGAA 2 cut(s) 8, 282
TspRI CASTG 1 cut(s) 418
VpaK11BI GGWCC 2 cut(s) 358, 369
XapI RAATTY 2 cut(s) 30, 281
XcmI CCANNNNNNNNNTGG 2 cut(s) 345, 379
XmiI GTMKAC 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.