Rw4G030370

omega-3 fatty acid desaturase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0023107
Physical Location & Seq
Forward (+)
57110255 .. 57110644
390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G030370.1

Sequence Viewer

Length: 390 bp
ATGGCTCTTCTTCGTCTTCGTCTTCTGGGGTTTTTCAGTTTGGTTTTTCCGTTTCGGCCTCACACCCAGATTTCACCGAGACCCAGAATCAGAATGGCTGCAGACCCAATAAAGCTTCCAACTTTGGAGAAGAACAACATCACAAATCTCGGATTTGGGTCGACCCGATTTTCAAGTCGGCACTTTAGAAGTTGGGGCATGAAGGTGAGTGCCCCCTTGAAAGTTGCTTCTACAGAAGATGGAGAAGAGAGAATGAGAGTCAATGGCGTTAATGGAGATGAAGTCAACATTGAATTTGATCCGGTTTCCCATCCACCTTTCAAATTGGCAGATGTTAAAGCAGCAACCCCAAAGAATTGTTGGGTTAAGGACCTATGGGGAGGTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3474 FA_desaturase

Protein Analysis

129

Amino Acids

14.53

Weight (kDa)

10.2

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3474 PF11960 18 - 126 2.6e-23 Domain of unknown function (DUF3474)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023107)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0004593.1_g000009
rosa_samantha Rh1BG271400 Rh1DG301700
rosa_wichuraiana Rw4G030370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 161
AclWI GGATC 1 cut(s) 293
AcsI RAATTY 1 cut(s) 293
AgsI TTSAA 4 cut(s) 174, 220, 293, 322
AluBI AGCT 1 cut(s) 115
AluI AGCT 1 cut(s) 115
Alw26I GTCTC 1 cut(s) 73
AlwI GGATC 1 cut(s) 293
AoxI GGCC 1 cut(s) 56
ApeKI GCWGC 2 cut(s) 98, 341
ApoI RAATTY 1 cut(s) 293
AspS9I GGNCC 2 cut(s) 370, 383
AsuHPI GGTGA 2 cut(s) 66, 217
AvaII GGWCC 2 cut(s) 370, 383
BaeGI GKGCMC 1 cut(s) 214
BbsI GAAGAC 2 cut(s) 8, 14
BbvI GCAGC 2 cut(s) 85, 353
BccI CCATC 2 cut(s) 233, 318
BcoDI GTCTC 1 cut(s) 73
BfmI CTRYAG 2 cut(s) 99, 231
BisI GCNGC 2 cut(s) 99, 342
BlsI GCNGC 2 cut(s) 100, 343
Bme18I GGWCC 2 cut(s) 370, 383
BmgT120I GGNCC 2 cut(s) 370, 383
BpiI GAAGAC 2 cut(s) 8, 14
BsaI GGTCTC 1 cut(s) 73
BsaWI WCCGGW 1 cut(s) 301
BsaXI ACNNNNNCTCC 2 cut(s) 267, 297
BseGI GGATG 1 cut(s) 310
BseSI GKGCMC 1 cut(s) 214
BseXI GCAGC 2 cut(s) 85, 353
BshFI GGCC 1 cut(s) 58
BsiSI CCGG 1 cut(s) 302
BsmAI GTCTC 1 cut(s) 73
BsnI GGCC 1 cut(s) 58
Bso31I GGTCTC 1 cut(s) 73
Bsp1286I GDGCHC 1 cut(s) 214
Bsp143I GATC 1 cut(s) 298
BspANI GGCC 1 cut(s) 58
BspMAI CTGCAG 1 cut(s) 103
BspPI GGATC 1 cut(s) 293
BspQI GCTCTTC 1 cut(s) 12
BspTNI GGTCTC 1 cut(s) 73
BssMI GATC 1 cut(s) 298
Bst6I CTCTTC 2 cut(s) 12, 240
BstF5I GGATG 1 cut(s) 310
BstKTI GATC 1 cut(s) 301
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 1 cut(s) 298
BstSFI CTRYAG 2 cut(s) 99, 231
BstSLI GKGCMC 1 cut(s) 214
BstV1I GCAGC 2 cut(s) 85, 353
BstV2I GAAGAC 2 cut(s) 8, 14
BsuRI GGCC 1 cut(s) 58
BtsCI GGATG 1 cut(s) 310
Cfr13I GGNCC 2 cut(s) 370, 383
CviAII CATG 2 cut(s) 199, 387
CviJI RGCY 4 cut(s) 5, 58, 98, 115
CviKI_1 RGCY 4 cut(s) 5, 58, 98, 115
DpnI GATC 1 cut(s) 300
DpnII GATC 1 cut(s) 298
Eam1104I CTCTTC 2 cut(s) 12, 240
EarI CTCTTC 2 cut(s) 12, 240
Eco31I GGTCTC 1 cut(s) 73
Eco47I GGWCC 2 cut(s) 370, 383
EcoO109I RGGNCCY 1 cut(s) 370
FaeI CATG 2 cut(s) 202, 390
FaiI YATR 3 cut(s) 200, 376, 388
FatI CATG 2 cut(s) 198, 386
FblI GTMKAC 1 cut(s) 161
Fnu4HI GCNGC 2 cut(s) 99, 342
FokI GGATG 1 cut(s) 297
Fsp4HI GCNGC 2 cut(s) 99, 342
GluI GCNGC 2 cut(s) 99, 342
HaeIII GGCC 1 cut(s) 58
HapII CCGG 1 cut(s) 302
Hin1II CATG 2 cut(s) 202, 390
HincII GTYRAC 2 cut(s) 162, 286
HindII GTYRAC 2 cut(s) 162, 286
HindIII AAGCTT 1 cut(s) 113
HinfI GANTC 2 cut(s) 87, 258
HpaII CCGG 1 cut(s) 302
HphI GGTGA 2 cut(s) 66, 217
Hpy166II GTNNAC 2 cut(s) 162, 286
Hpy188I TCNGA 2 cut(s) 92, 152
Hpy8I GTNNAC 2 cut(s) 162, 286
HpyAV CCTTC 1 cut(s) 196
HpyCH4V TGCA 1 cut(s) 101
Hsp92II CATG 2 cut(s) 202, 390
Kzo9I GATC 1 cut(s) 298
LguI GCTCTTC 1 cut(s) 12
LpnPI CCDG 4 cut(s) 11, 80, 97, 315
Lsp1109I GCAGC 2 cut(s) 85, 353
MalI GATC 1 cut(s) 300
MboI GATC 1 cut(s) 298
MboII GAAGA 5 cut(s) 8, 14, 142, 248, 257
MhlI GDGCHC 1 cut(s) 214
MluCI AATT 3 cut(s) 293, 323, 355
MlyI GAGTC 1 cut(s) 267
MmeI TCCRAC 1 cut(s) 143
MnlI CCTC 2 cut(s) 69, 374
MseI TTAA 3 cut(s) 270, 336, 366
MslI CAYNNNNRTG 1 cut(s) 203
MspI CCGG 1 cut(s) 302
NdeII GATC 1 cut(s) 298
NlaIII CATG 2 cut(s) 202, 390
PciSI GCTCTTC 1 cut(s) 12
PfeI GAWTC 1 cut(s) 87
PkrI GCNGC 2 cut(s) 100, 343
PleI GAGTC 1 cut(s) 266
PpsI GAGTC 1 cut(s) 266
PpuMI RGGWCCY 1 cut(s) 370
Psp5II RGGWCCY 1 cut(s) 370
PspPI GGNCC 2 cut(s) 370, 383
PspPPI RGGWCCY 1 cut(s) 370
PstI CTGCAG 1 cut(s) 103
RseI CAYNNNNRTG 1 cut(s) 203
SalI GTCGAC 1 cut(s) 160
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 3 cut(s) 270, 336, 366
SatI GCNGC 2 cut(s) 99, 342
Sau3AI GATC 1 cut(s) 298
Sau96I GGNCC 2 cut(s) 370, 383
SchI GAGTC 1 cut(s) 267
SduI GDGCHC 1 cut(s) 214
SetI ASST 5 cut(s) 117, 207, 319, 375, 385
SfcI CTRYAG 2 cut(s) 99, 231
SinI GGWCC 2 cut(s) 370, 383
SmiMI CAYNNNNRTG 1 cut(s) 203
Sse9I AATT 3 cut(s) 293, 323, 355
TaqI TCGA 1 cut(s) 161
TasI AATT 3 cut(s) 293, 323, 355
TfiI GAWTC 1 cut(s) 87
Tru1I TTAA 3 cut(s) 270, 336, 366
Tru9I TTAA 3 cut(s) 270, 336, 366
TseI GCWGC 2 cut(s) 98, 341
TspDTI ATGAA 2 cut(s) 215, 294
TspGWI ACGGA 1 cut(s) 39
VpaK11BI GGWCC 2 cut(s) 370, 383
XapI RAATTY 1 cut(s) 293
XcmI CCANNNNNNNNNTGG 2 cut(s) 91, 357
XmiI GTMKAC 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.