Rh1CG359300

Hydroxyacylglutathione hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0023028
Physical Location & Seq
Forward (+)
63277772 .. 63280628
2857 bp
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UTR
Exon/CDS
Intron
Rh1CG359300.1

Sequence Viewer

Length: 561 bp
ATGAAAATCCATCACGTTGCTTGCTTGGAAGACAATTACGCATACCTAATTATTGACGAGAGCACCAAAGAAGCCGCGGTTGTGGATCCTGTTGAACCCGAAAAGGTTCTTAAAGCAGCCCAAGACCATGGTGCCCAAGTCAAACTCGTCCTCACCACTCACCACCATTGGGATCATGCCGGAGGAAATGAGAAGATAAAGCAGTTGGTGCCTGGCATTAAAGTGTATGGGGGTTCTGTTGATAATGTGAAGGGCTGCACTGATAAGGTGGACAATGGTGATAAGATTTCGCTCGGAGCTGATACTCAAATACTCTCGCTTCACACACCTTGTCACACAAAAGGTCACATAAGTTATTACGTGACAAGCAAAGAGGGAGAGGAGCCAGCTGTTTTCACCGGAGACACACTGGTAAGTTATGAAAGAAGCTTTAGTTTCTCTGCAACCAATAATATCAGCAGATGTCAGGAAAAGCCCTTTAGGGTACCTATTTCCAAGAGACTAATTTGTCTGAATGCACTGTGCAAGAGAAATGAGTTATTGTATTGCACTATGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00620 map00620
Pfam Domains
Protein Families
HAGH_C Lactamase_B

Protein Analysis

186

Amino Acids

20.62

Weight (kDa)

6.78

Isoelectric Point (pI)

31.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lactamase_B PF00753 13 - 141 6.3e-12 Metallo-beta-lactamase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023028)

Species Orthologous Gene IDs
rosa_multiflora Rmu_ssc0000419.1_g000001
rosa_samantha Rh1AG382500 Rh1CG359300 Rh1DG377100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 507
Acc65I GGTACC 1 cut(s) 484
AccB1I GGYRCC 3 cut(s) 131, 208, 484
AccII CGCG 1 cut(s) 77
AciI CCGC 2 cut(s) 75, 77
AclWI GGATC 3 cut(s) 80, 93, 180
AfaI GTAC 1 cut(s) 486
AfiI CCNNNNNNNGG 1 cut(s) 169
AgsI TTSAA 1 cut(s) 95
AjnI CCWGG 1 cut(s) 211
AluBI AGCT 3 cut(s) 299, 389, 429
AluI AGCT 3 cut(s) 299, 389, 429
Alw21I GWGCWC 1 cut(s) 65
Alw26I GTCTC 2 cut(s) 396, 493
AlwI GGATC 3 cut(s) 80, 93, 180
ApeKI GCWGC 2 cut(s) 116, 255
Asp700I GAANNNNTTC 1 cut(s) 105
Asp718I GGTACC 1 cut(s) 484
AsuHPI GGTGA 4 cut(s) 145, 152, 290, 388
BaeGI GKGCMC 1 cut(s) 136
BamHI GGATCC 1 cut(s) 85
BanI GGYRCC 3 cut(s) 131, 208, 484
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 65
BbvI GCAGC 2 cut(s) 128, 242
BccI CCATC 1 cut(s) 18
BciT130I CCWGG 1 cut(s) 213
BcoDI GTCTC 2 cut(s) 396, 493
BisI GCNGC 3 cut(s) 75, 117, 256
BlsI GCNGC 3 cut(s) 76, 118, 257
Bme1390I CCNGG 1 cut(s) 213
BmiI GGNNCC 5 cut(s) 87, 133, 210, 384, 486
BmrFI CCNGG 1 cut(s) 213
BpiI GAAGAC 1 cut(s) 36
BsaAI YACGTR 1 cut(s) 361
BsaJI CCNNGG 2 cut(s) 75, 127
BsaWI WCCGGW 1 cut(s) 398
Bsc4I CCNNNNNNNGG 1 cut(s) 169
Bse1I ACTGG 1 cut(s) 414
BseBI CCWGG 1 cut(s) 213
BseDI CCNNGG 2 cut(s) 75, 127
BseLI CCNNNNNNNGG 1 cut(s) 169
BseNI ACTGG 1 cut(s) 414
BseRI GAGGAG 1 cut(s) 395
BseSI GKGCMC 1 cut(s) 136
BseXI GCAGC 2 cut(s) 128, 242
BsgI GTGCAG 1 cut(s) 241
Bsh1236I CGCG 1 cut(s) 77
BshNI GGYRCC 3 cut(s) 131, 208, 484
BsiHKAI GWGCWC 1 cut(s) 65
BsiSI CCGG 2 cut(s) 180, 399
BslI CCNNNNNNNGG 1 cut(s) 169
BsmAI GTCTC 2 cut(s) 396, 493
BsmI GAATGC 1 cut(s) 520
Bsp1286I GDGCHC 2 cut(s) 65, 136
Bsp143I GATC 2 cut(s) 85, 172
Bsp19I CCATGG 1 cut(s) 127
BspACI CCGC 2 cut(s) 75, 77
BspFNI CGCG 1 cut(s) 77
BspLI GGNNCC 5 cut(s) 87, 133, 210, 384, 486
BspPI GGATC 3 cut(s) 80, 93, 180
BspT107I GGYRCC 3 cut(s) 131, 208, 484
BsrI ACTGG 1 cut(s) 414
BssECI CCNNGG 2 cut(s) 75, 127
BssMI GATC 2 cut(s) 85, 172
BssT1I CCWWGG 1 cut(s) 127
Bst2UI CCWGG 1 cut(s) 213
Bst4CI ACNGT 1 cut(s) 522
BstAPI GCANNNNNTGC 1 cut(s) 208
BstBAI YACGTR 1 cut(s) 361
BstC8I GCNNGC 2 cut(s) 22, 387
BstDSI CCRYGG 2 cut(s) 75, 127
BstFNI CGCG 1 cut(s) 77
BstKTI GATC 2 cut(s) 88, 175
BstMAI GTCTC 2 cut(s) 396, 493
BstMBI GATC 2 cut(s) 85, 172
BstMWI GCNNNNNNNGC 1 cut(s) 208
BstNI CCWGG 1 cut(s) 213
BstSCI CCNGG 1 cut(s) 211
BstSLI GKGCMC 1 cut(s) 136
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 2 cut(s) 128, 242
BstV2I GAAGAC 1 cut(s) 36
BstX2I RGATCY 1 cut(s) 85
BstXI CCANNNNNNTGG 1 cut(s) 128
BstYI RGATCY 1 cut(s) 85
BtgI CCRYGG 2 cut(s) 75, 127
BtsIMutI CAGTG 3 cut(s) 258, 407, 518
Cac8I GCNNGC 2 cut(s) 22, 387
Cfr42I CCGCGG 1 cut(s) 78
Csp6I GTAC 1 cut(s) 485
CviAII CATG 2 cut(s) 128, 176
CviJI RGCY 8 cut(s) 74, 119, 255, 299, 385, 389, 429, 475
CviKI_1 RGCY 8 cut(s) 74, 119, 255, 299, 385, 389, 429, 475
CviQI GTAC 1 cut(s) 485
DpnI GATC 2 cut(s) 87, 174
DpnII GATC 2 cut(s) 85, 172
DrdI GACNNNNNNGTC 1 cut(s) 507
DseDI GACNNNNNNGTC 1 cut(s) 507
Eco130I CCWWGG 1 cut(s) 127
EcoRII CCWGG 1 cut(s) 211
EcoT14I CCWWGG 1 cut(s) 127
ErhI CCWWGG 1 cut(s) 127
FaeI CATG 2 cut(s) 131, 179
FaiI YATR 7 cut(s) 43, 129, 177, 228, 350, 420, 554
FatI CATG 2 cut(s) 127, 175
Fnu4HI GCNGC 3 cut(s) 75, 117, 256
Fsp4HI GCNGC 3 cut(s) 75, 117, 256
GluI GCNGC 3 cut(s) 75, 117, 256
HapII CCGG 2 cut(s) 180, 399
Hin1II CATG 2 cut(s) 131, 179
HindIII AAGCTT 1 cut(s) 427
HpaII CCGG 2 cut(s) 180, 399
HphI GGTGA 4 cut(s) 145, 152, 290, 388
Hpy166II GTNNAC 1 cut(s) 271
Hpy188I TCNGA 2 cut(s) 296, 513
Hpy188III TCNNGA 1 cut(s) 467
Hpy8I GTNNAC 1 cut(s) 271
HpyAV CCTTC 1 cut(s) 244
HpyCH4III ACNGT 1 cut(s) 522
HpyCH4IV ACGT 2 cut(s) 15, 360
HpyCH4V TGCA 5 cut(s) 258, 443, 518, 525, 549
HpyF10VI GCNNNNNNNGC 1 cut(s) 208
HpySE526I ACGT 2 cut(s) 15, 360
Hsp92II CATG 2 cut(s) 131, 179
KpnI GGTACC 1 cut(s) 488
KspI CCGCGG 1 cut(s) 78
Kzo9I GATC 2 cut(s) 85, 172
LmnI GCTCC 2 cut(s) 296, 382
LpnPI CCDG 8 cut(s) 102, 193, 198, 225, 395, 399, 412, 452
Lsp1109I GCAGC 2 cut(s) 128, 242
MaeII ACGT 2 cut(s) 15, 360
MaeIII GTNAC 3 cut(s) 332, 344, 361
MalI GATC 2 cut(s) 87, 174
MboI GATC 2 cut(s) 85, 172
MboII GAAGA 2 cut(s) 41, 205
MflI RGATCY 1 cut(s) 85
MhlI GDGCHC 2 cut(s) 65, 136
MluCI AATT 3 cut(s) 34, 48, 504
MnlI CCTC 4 cut(s) 161, 176, 367, 373
MroXI GAANNNNTTC 1 cut(s) 105
MseI TTAA 2 cut(s) 111, 219
MslI CAYNNNNRTG 1 cut(s) 221
MspA1I CMGCKG 2 cut(s) 77, 389
MspI CCGG 2 cut(s) 180, 399
MspR9I CCNGG 1 cut(s) 213
Mva1269I GAATGC 1 cut(s) 520
MvaI CCWGG 1 cut(s) 213
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 1 cut(s) 208
NcoI CCATGG 1 cut(s) 127
NdeII GATC 2 cut(s) 85, 172
NlaIII CATG 2 cut(s) 131, 179
NlaIV GGNNCC 5 cut(s) 87, 133, 210, 384, 486
NmuCI GTSAC 3 cut(s) 332, 344, 361
PctI GAATGC 1 cut(s) 520
PdmI GAANNNNTTC 1 cut(s) 105
PkrI GCNGC 3 cut(s) 76, 118, 257
Ppu21I YACGTR 1 cut(s) 361
Psp6I CCWGG 1 cut(s) 211
PspGI CCWGG 1 cut(s) 211
PspN4I GGNNCC 5 cut(s) 87, 133, 210, 384, 486
PsuI RGATCY 1 cut(s) 85
PvuII CAGCTG 1 cut(s) 389
RsaI GTAC 1 cut(s) 486
RsaNI GTAC 1 cut(s) 485
RseI CAYNNNNRTG 1 cut(s) 221
SacII CCGCGG 1 cut(s) 78
SaqAI TTAA 2 cut(s) 111, 219
SatI GCNGC 3 cut(s) 75, 117, 256
Sau3AI GATC 2 cut(s) 85, 172
ScrFI CCNGG 1 cut(s) 213
SduI GDGCHC 2 cut(s) 65, 136
Sfr303I CCGCGG 1 cut(s) 78
SgrBI CCGCGG 1 cut(s) 78
SmiMI CAYNNNNRTG 1 cut(s) 221
Sse9I AATT 3 cut(s) 34, 48, 504
SsiI CCGC 2 cut(s) 75, 77
StyD4I CCNGG 1 cut(s) 211
StyI CCWWGG 1 cut(s) 127
TaaI ACNGT 1 cut(s) 522
TaiI ACGT 2 cut(s) 18, 363
TasI AATT 3 cut(s) 34, 48, 504
TauI GCSGC 1 cut(s) 77
Tru1I TTAA 2 cut(s) 111, 219
Tru9I TTAA 2 cut(s) 111, 219
TscAI CASTG 3 cut(s) 265, 414, 525
TseFI GTSAC 3 cut(s) 332, 344, 361
TseI GCWGC 2 cut(s) 116, 255
Tsp45I GTSAC 3 cut(s) 332, 344, 361
TspDTI ATGAA 2 cut(s) 17, 435
TspRI CASTG 3 cut(s) 265, 414, 525
XmnI GAANNNNTTC 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.