Rh1CG390000

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0022275
Physical Location & Seq
Forward (+)
65884956 .. 65889421
4466 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG390000.1

Sequence Viewer

Length: 909 bp
ATGGGGAATTCATTTGGGTGCTCTGCCTCCGGCGAGAGGCTGGTATCGGCGGCGAGAGATGGAGATGTGATTGAAGCGAAGATGTTATTGGATTGCAACCCTTGCCTTGCTAAGTACTCAACCTTTGGTGGGCTTAACTCTCCTCTCCATTTCGCCGCCGCTAAAGGCCACAACGAGATTGTTTCGTTGCTTCTGGAGAATGGAGCTGATGTCAATTCCAGAAATTACTGTGGCCAGACAGCTTTGATGCAAGCTTGTCGATATGGACATTGGGAAGTTTTGCAAACGCTCCTGCTGTTTAGGTGCAATGTTACAAGAGCAGACTACCTTACAGGCAGAACAGCTCTTCATTTTGCTGCTGTAAATGGACATGTAAGATGTATAAGACTTGTTGTGGCTGACTTTGTTCCAAGTGTTCCTTATGAAGCTATGAATTCTCAGATAGATAGTGACAGAGGTGATGGTGCAAATGCGACAAGTAAAAATGAGCAAAGTGCTCTGTCCAAGTTTGTAAATAAGCCAGCAGATTGTGGCATTACCGCTCTTCATATGGCTGCATTGAATGGGTATTTTGATTGCGTACAGCTCCTACTTGATCTTCATGCAAATGTGGCAGCTGTTACATTTCATTATGGAACGTCTATGGATTTAATAGGAGCCGGAAGTTCTCCATTGCACTACGCTGCTTGTGGGGGTAACTTAAAATGCTGTCAGATCCTCCTTGCAAGAGGTGCTAGCAAGATGACTTTAAATTGCAATGGGTGGCTTCCTCTTGATGTTGCCAGAATGTGGGGGCGCCACTGGCTTGAACCGCTGCTAGCACCCAATTCTGACACAATAATACCGGCATTCCCCCGTTCTAATTACCTATCACTGCCTCTCATGAGCATACTGAACGTAGCAAGGTAA

Protein Analysis

302

Amino Acids

32.57

Weight (kDa)

7.1

Isoelectric Point (pI)

33.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 16 - 106 3.2e-14 Ankyrin repeats (3 copies)
Ank PF00023 46 - 75 4.3e-09 Ankyrin repeat
Ank_3 PF13606 46 - 72 7.8e-06 Ankyrin repeat
Ank_2 PF12796 49 - 132 3.4e-18 Ankyrin repeats (3 copies)
Ank_4 PF13637 49 - 98 1.7e-13 Ankyrin repeats (many copies)
Ank_5 PF13857 64 - 109 2.1e-09 Ankyrin repeats (many copies)
Ank_2 PF12796 107 - 204 1.1e-09 Ankyrin repeats (3 copies)
Ank_4 PF13637 171 - 198 1e-05 Ankyrin repeats (many copies)
Ank_5 PF13857 172 - 217 1e-06 Ankyrin repeats (many copies)
Ank PF00023 178 - 207 1.2e-06 Ankyrin repeat
Ank_2 PF12796 220 - 268 5.6e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022275)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g29140 FvH4_7g29140
pyrus_communis pycom01g20840
rosa_samantha Rh1CG390000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 795
AccB7I CCANNNNNTGG 1 cut(s) 789
AccBSI CCGCTC 1 cut(s) 542
AciI CCGC 5 cut(s) 50, 156, 159, 540, 812
AclWI GGATC 1 cut(s) 709
AcoI YGGCCR 1 cut(s) 232
AcsI RAATTY 2 cut(s) 7, 433
AcyI GRCGYC 1 cut(s) 796
AfaI GTAC 2 cut(s) 116, 582
AfiI CCNNNNNNNGG 3 cut(s) 36, 129, 789
AflIII ACRYGT 1 cut(s) 370
AgsI TTSAA 3 cut(s) 74, 562, 809
AjuI GAANNNNNNNTTGG 2 cut(s) 71, 103
AluBI AGCT 7 cut(s) 206, 242, 254, 344, 428, 586, 617
AluI AGCT 7 cut(s) 206, 242, 254, 344, 428, 586, 617
Alw21I GWGCWC 2 cut(s) 23, 499
AlwI GGATC 1 cut(s) 709
AoxI GGCC 2 cut(s) 166, 232
ApeKI GCWGC 5 cut(s) 356, 554, 614, 683, 814
ApoI RAATTY 2 cut(s) 7, 433
AspLEI GCGC 1 cut(s) 798
AsuHPI GGTGA 1 cut(s) 470
AsuNHI GCTAGC 2 cut(s) 734, 817
BalI TGGCCA 1 cut(s) 234
BanI GGYRCC 1 cut(s) 795
Bbv12I GWGCWC 2 cut(s) 23, 499
BbvI GCAGC 5 cut(s) 343, 541, 626, 670, 801
BccI CCATC 2 cut(s) 53, 455
BcgI CGANNNNNNTGC 2 cut(s) 239, 273
BfaI CTAG 2 cut(s) 735, 818
BfoI RGCGCY 1 cut(s) 799
BisI GCNGC 8 cut(s) 51, 156, 159, 357, 555, 615, 684, 815
BlsI GCNGC 8 cut(s) 52, 157, 160, 358, 556, 616, 685, 816
BmcAI AGTACT 1 cut(s) 116
BmiI GGNNCC 2 cut(s) 658, 797
BmsI GCATC 1 cut(s) 237
BmtI GCTAGC 2 cut(s) 738, 821
BpmI CTGGAG 1 cut(s) 215
BsaHI GRCGYC 1 cut(s) 796
BsaXI ACNNNNNCTCC 2 cut(s) 195, 225
Bsc4I CCNNNNNNNGG 3 cut(s) 36, 129, 789
Bse118I RCCGGY 1 cut(s) 844
Bse1I ACTGG 1 cut(s) 806
Bse3DI GCAATG 3 cut(s) 313, 671, 763
BseLI CCNNNNNNNGG 3 cut(s) 36, 129, 789
BseMI GCAATG 3 cut(s) 313, 671, 763
BseMII CTCAG 1 cut(s) 452
BseNI ACTGG 1 cut(s) 806
BseRI GAGGAG 1 cut(s) 132
BseXI GCAGC 5 cut(s) 343, 541, 626, 670, 801
BshFI GGCC 2 cut(s) 168, 234
BshNI GGYRCC 1 cut(s) 795
BsiHKAI GWGCWC 2 cut(s) 23, 499
BsiSI CCGG 3 cut(s) 30, 660, 845
BslI CCNNNNNNNGG 3 cut(s) 36, 129, 789
BsmI GAATGC 1 cut(s) 848
BsnI GGCC 2 cut(s) 168, 234
Bsp1286I GDGCHC 2 cut(s) 23, 499
Bsp143I GATC 2 cut(s) 595, 714
BspACI CCGC 5 cut(s) 50, 156, 159, 540, 812
BspANI GGCC 2 cut(s) 168, 234
BspCNI CTCAG 1 cut(s) 451
BspHI TCATGA 1 cut(s) 882
BspLI GGNNCC 2 cut(s) 658, 797
BspOI GCTAGC 2 cut(s) 738, 821
BspPI GGATC 1 cut(s) 709
BspQI GCTCTTC 2 cut(s) 351, 549
BspT107I GGYRCC 1 cut(s) 795
BsrBI CCGCTC 1 cut(s) 542
BsrDI GCAATG 3 cut(s) 313, 671, 763
BsrFI RCCGGY 1 cut(s) 844
BsrI ACTGG 1 cut(s) 806
BssAI RCCGGY 1 cut(s) 844
BssMI GATC 2 cut(s) 595, 714
BssNI GRCGYC 1 cut(s) 796
Bst4CI ACNGT 1 cut(s) 230
Bst6I CTCTTC 2 cut(s) 351, 549
BstACI GRCGYC 1 cut(s) 796
BstAPI GCANNNNNTGC 2 cut(s) 102, 731
BstC8I GCNNGC 4 cut(s) 252, 522, 736, 819
BstDEI CTNAG 2 cut(s) 111, 438
BstH2I RGCGCY 1 cut(s) 799
BstHHI GCGC 1 cut(s) 798
BstKTI GATC 2 cut(s) 598, 717
BstMBI GATC 2 cut(s) 595, 714
BstMWI GCNNNNNNNGC 5 cut(s) 102, 611, 731, 802, 811
BstNSI RCATGY 1 cut(s) 374
BstV1I GCAGC 5 cut(s) 343, 541, 626, 670, 801
BstX2I RGATCY 1 cut(s) 714
BstYI RGATCY 1 cut(s) 714
BsuRI GGCC 2 cut(s) 168, 234
BtsI GCAGTG 1 cut(s) 872
BtsIMutI CAGTG 2 cut(s) 799, 872
Cac8I GCNNGC 4 cut(s) 252, 522, 736, 819
CciI TCATGA 1 cut(s) 882
CfoI GCGC 1 cut(s) 798
Cfr10I RCCGGY 1 cut(s) 844
Csp6I GTAC 2 cut(s) 115, 581
CviAII CATG 3 cut(s) 371, 602, 883
CviQI GTAC 2 cut(s) 115, 581
DdeI CTNAG 2 cut(s) 111, 438
DinI GGCGCC 1 cut(s) 797
DpnI GATC 2 cut(s) 597, 716
DpnII GATC 2 cut(s) 595, 714
DraI TTTAAA 1 cut(s) 750
EaeI YGGCCR 1 cut(s) 232
Eam1104I CTCTTC 2 cut(s) 351, 549
EarI CTCTTC 2 cut(s) 351, 549
EcoRI GAATTC 2 cut(s) 7, 433
EgeI GGCGCC 1 cut(s) 797
EheI GGCGCC 1 cut(s) 797
FaeI CATG 3 cut(s) 374, 605, 886
FalI AAGNNNNNCTT 2 cut(s) 403, 435
FatI CATG 3 cut(s) 370, 601, 882
FauNDI CATATG 1 cut(s) 549
Fnu4HI GCNGC 8 cut(s) 51, 156, 159, 357, 555, 615, 684, 815
Fsp4HI GCNGC 8 cut(s) 51, 156, 159, 357, 555, 615, 684, 815
FspBI CTAG 2 cut(s) 735, 818
GlaI GCGC 1 cut(s) 797
GluI GCNGC 8 cut(s) 51, 156, 159, 357, 555, 615, 684, 815
GsuI CTGGAG 1 cut(s) 215
HaeII RGCGCY 1 cut(s) 799
HaeIII GGCC 2 cut(s) 168, 234
HapII CCGG 3 cut(s) 30, 660, 845
HhaI GCGC 1 cut(s) 798
Hin1I GRCGYC 1 cut(s) 796
Hin1II CATG 3 cut(s) 374, 605, 886
Hin6I GCGC 1 cut(s) 796
HinP1I GCGC 1 cut(s) 796
HindIII AAGCTT 1 cut(s) 252
HpaII CCGG 3 cut(s) 30, 660, 845
HphI GGTGA 1 cut(s) 470
Hpy188I TCNGA 3 cut(s) 441, 714, 832
Hpy188III TCNNGA 4 cut(s) 194, 219, 773, 883
HpyCH4III ACNGT 1 cut(s) 230
HpyCH4IV ACGT 2 cut(s) 638, 897
HpyF10VI GCNNNNNNNGC 5 cut(s) 102, 611, 731, 802, 811
HpyF3I CTNAG 2 cut(s) 111, 438
HpySE526I ACGT 2 cut(s) 638, 897
Hsp92I GRCGYC 1 cut(s) 796
Hsp92II CATG 3 cut(s) 374, 605, 886
HspAI GCGC 1 cut(s) 796
KasI GGCGCC 1 cut(s) 795
Kzo9I GATC 2 cut(s) 595, 714
LguI GCTCTTC 2 cut(s) 351, 549
LmnI GCTCC 4 cut(s) 203, 294, 591, 656
Lsp1109I GCAGC 5 cut(s) 343, 541, 626, 670, 801
LweI GCATC 1 cut(s) 237
MaeI CTAG 2 cut(s) 735, 818
MaeII ACGT 2 cut(s) 638, 897
MaeIII GTNAC 4 cut(s) 310, 449, 619, 695
MalI GATC 2 cut(s) 597, 716
MbiI CCGCTC 1 cut(s) 542
MboI GATC 2 cut(s) 595, 714
MboII GAAGA 4 cut(s) 91, 338, 536, 590
MflI RGATCY 1 cut(s) 714
MhlI GDGCHC 2 cut(s) 23, 499
MlsI TGGCCA 1 cut(s) 234
MluCI AATT 7 cut(s) 7, 214, 223, 433, 751, 826, 862
MluNI TGGCCA 1 cut(s) 234
Mly113I GGCGCC 1 cut(s) 796
MnlI CCTC 8 cut(s) 30, 37, 153, 449, 722, 728, 780, 888
Mox20I TGGCCA 1 cut(s) 234
MscI TGGCCA 1 cut(s) 234
MseI TTAA 4 cut(s) 135, 650, 701, 749
MslI CAYNNNNRTG 2 cut(s) 16, 606
Msp20I TGGCCA 1 cut(s) 234
MspA1I CMGCKG 2 cut(s) 617, 814
MspI CCGG 3 cut(s) 30, 660, 845
Mva1269I GAATGC 1 cut(s) 848
MwoI GCNNNNNNNGC 5 cut(s) 102, 611, 731, 802, 811
NarI GGCGCC 1 cut(s) 796
NdeI CATATG 1 cut(s) 549
NdeII GATC 2 cut(s) 595, 714
NheI GCTAGC 2 cut(s) 734, 817
NlaIII CATG 3 cut(s) 374, 605, 886
NlaIV GGNNCC 2 cut(s) 658, 797
NmuCI GTSAC 1 cut(s) 449
NspI RCATGY 1 cut(s) 374
PagI TCATGA 1 cut(s) 882
PciI ACATGT 1 cut(s) 370
PciSI GCTCTTC 2 cut(s) 351, 549
PctI GAATGC 1 cut(s) 848
PflMI CCANNNNNTGG 1 cut(s) 789
PkrI GCNGC 8 cut(s) 52, 157, 160, 358, 556, 616, 685, 816
PluTI GGCGCC 1 cut(s) 799
PscI ACATGT 1 cut(s) 370
PspN4I GGNNCC 2 cut(s) 658, 797
PsuI RGATCY 1 cut(s) 714
PvuII CAGCTG 1 cut(s) 617
RsaI GTAC 2 cut(s) 116, 582
RsaNI GTAC 2 cut(s) 115, 581
RseI CAYNNNNRTG 2 cut(s) 16, 606
SapI GCTCTTC 2 cut(s) 351, 549
SaqAI TTAA 4 cut(s) 135, 650, 701, 749
SatI GCNGC 8 cut(s) 51, 156, 159, 357, 555, 615, 684, 815
Sau3AI GATC 2 cut(s) 595, 714
ScaI AGTACT 1 cut(s) 116
SduI GDGCHC 2 cut(s) 23, 499
SfaNI GCATC 1 cut(s) 237
SfoI GGCGCC 1 cut(s) 797
SmiMI CAYNNNNRTG 2 cut(s) 16, 606
Sse9I AATT 7 cut(s) 7, 214, 223, 433, 751, 826, 862
SsiI CCGC 5 cut(s) 50, 156, 159, 540, 812
SspDI GGCGCC 1 cut(s) 795
SspMI CTAG 2 cut(s) 735, 818
TaaI ACNGT 1 cut(s) 230
TaiI ACGT 2 cut(s) 641, 900
TaqI TCGA 1 cut(s) 259
TasI AATT 7 cut(s) 7, 214, 223, 433, 751, 826, 862
TatI WGTACW 1 cut(s) 114
TauI GCSGC 3 cut(s) 53, 158, 161
Tru1I TTAA 4 cut(s) 135, 650, 701, 749
Tru9I TTAA 4 cut(s) 135, 650, 701, 749
TscAI CASTG 2 cut(s) 806, 879
TseFI GTSAC 1 cut(s) 449
TseI GCWGC 5 cut(s) 356, 554, 614, 683, 814
Tsp45I GTSAC 1 cut(s) 449
TspDTI ATGAA 6 cut(s) 338, 438, 446, 536, 590, 617
TspRI CASTG 2 cut(s) 806, 879
Van91I CCANNNNNTGG 1 cut(s) 789
XapI RAATTY 2 cut(s) 7, 433
XceI RCATGY 1 cut(s) 374
XspI CTAG 2 cut(s) 735, 818
ZrmI AGTACT 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.