Rh1DG398000

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0026204
Physical Location & Seq
Forward (+)
61736744 .. 61760459
23716 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG398000.1

Sequence Viewer

Length: 660 bp
ATGTCGACAGTCATCGGAATCTGGGACTCCGGTCCAACCGTAACTTGTGGCAGAACGGTTGATGTTGACGATGTTGTAATCGGAATAGTGGCGTTGCGAATTCTAATCATGGAGGATTGTGGATTGTGTTATGATAAAGAGGAAAGGAGGAAACGAAAAGGGAGTCTGGCAAAGTATCAATCGTACAATGTTATCAAAGGTGATCACATCTTACAACCAGTGATAGCATTCGAACCTCATCCTTCCATCACAGAAAGGGAGAATCGTATCAAGATGACCTACAGTATATACGGAATAGTGGCTTTGCGAATTCTAAGCATGGAGAGTTGTAGATTGTGGTCTCTACAGGGAAAGGGGATTGTTCGGGATTGTGTTCGATACGAAGATGAACATGAACCTGGGAACCGCAGTAGATTAGAGGGTTACCAGGGAATTAGGTGTTTGTCAACTCAAGACCAGTGCCAGGAAAGCCTTTCCCAAAAGGGTTCTAGCATTTCCGTCTTTGGGGGAGGACCATATGTAATAACAAAGCAATCCAAGATTCCAAGGTACACAACTGGATTCGGGCTGGCTCTCTACGTGCACCTCAATGATCTTGTCTCCTGGGCTTGCCAAGATGAACAGTGGTCTCGATTGCTTGATAAGGCTAATTCAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

219

Amino Acids

24.7

Weight (kDa)

7.54

Isoelectric Point (pI)

44.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0026204)

Species Orthologous Gene IDs
rosa_samantha Rh1AG404600 Rh1CG381000 Rh1DG398000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 5
AciI CCGC 1 cut(s) 406
AcsI RAATTY 2 cut(s) 99, 309
AfaI GTAC 2 cut(s) 185, 551
AfiI CCNNNNNNNGG 2 cut(s) 463, 504
AgsI TTSAA 1 cut(s) 654
AjnI CCWGG 4 cut(s) 397, 426, 462, 602
Alw21I GWGCWC 1 cut(s) 585
Alw26I GTCTC 3 cut(s) 345, 604, 633
Alw44I GTGCAC 1 cut(s) 581
ApaLI GTGCAC 1 cut(s) 581
ApoI RAATTY 2 cut(s) 99, 309
ArsI GACNNNNNNTTYG 2 cut(s) 148, 180
AspS9I GGNCC 2 cut(s) 32, 512
AsuHPI GGTGA 1 cut(s) 212
AsuII TTCGAA 1 cut(s) 231
AvaII GGWCC 2 cut(s) 32, 512
BaeGI GKGCMC 1 cut(s) 585
Bbv12I GWGCWC 1 cut(s) 585
BccI CCATC 1 cut(s) 254
BciT130I CCWGG 4 cut(s) 399, 428, 464, 604
BclI TGATCA 1 cut(s) 202
BcoDI GTCTC 3 cut(s) 345, 604, 633
BfaI CTAG 1 cut(s) 489
BfmI CTRYAG 2 cut(s) 280, 344
Bme1390I CCNGG 4 cut(s) 399, 428, 464, 604
Bme18I GGWCC 2 cut(s) 32, 512
BmgT120I GGNCC 2 cut(s) 32, 512
BmiI GGNNCC 1 cut(s) 404
BmrFI CCNGG 4 cut(s) 399, 428, 464, 604
BoxI GACNNNNGTC 1 cut(s) 30
Bpu14I TTCGAA 1 cut(s) 231
BpuEI CTTGAG 1 cut(s) 435
BsaAI YACGTR 1 cut(s) 580
BsaI GGTCTC 2 cut(s) 345, 633
BsaJI CCNNGG 4 cut(s) 398, 427, 545, 603
BsaWI WCCGGW 1 cut(s) 29
Bsc4I CCNNNNNNNGG 2 cut(s) 463, 504
Bse1I ACTGG 3 cut(s) 218, 457, 562
BseBI CCWGG 4 cut(s) 399, 428, 464, 604
BseDI CCNNGG 4 cut(s) 398, 427, 545, 603
BseGI GGATG 1 cut(s) 238
BseLI CCNNNNNNNGG 2 cut(s) 463, 504
BseNI ACTGG 3 cut(s) 218, 457, 562
BseSI GKGCMC 1 cut(s) 585
BsiHKAI GWGCWC 1 cut(s) 585
BsiSI CCGG 1 cut(s) 30
BslFI GGGAC 1 cut(s) 38
BslI CCNNNNNNNGG 2 cut(s) 463, 504
BsmAI GTCTC 3 cut(s) 345, 604, 633
BsmFI GGGAC 1 cut(s) 38
BsmI GAATGC 1 cut(s) 227
Bso31I GGTCTC 2 cut(s) 345, 633
Bsp119I TTCGAA 1 cut(s) 231
Bsp1286I GDGCHC 1 cut(s) 585
Bsp143I GATC 2 cut(s) 202, 592
BspACI CCGC 1 cut(s) 406
BspLI GGNNCC 1 cut(s) 404
BspT104I TTCGAA 1 cut(s) 231
BspTNI GGTCTC 2 cut(s) 345, 633
BsrI ACTGG 3 cut(s) 218, 457, 562
BssECI CCNNGG 4 cut(s) 398, 427, 545, 603
BssMI GATC 2 cut(s) 202, 592
BssT1I CCWWGG 1 cut(s) 545
Bst2UI CCWGG 4 cut(s) 399, 428, 464, 604
Bst4CI ACNGT 5 cut(s) 10, 40, 58, 284, 624
BstBAI YACGTR 1 cut(s) 580
BstBI TTCGAA 1 cut(s) 231
BstC8I GCNNGC 2 cut(s) 570, 610
BstDEI CTNAG 1 cut(s) 314
BstEII GGTNACC 1 cut(s) 422
BstF5I GGATG 1 cut(s) 238
BstKTI GATC 2 cut(s) 205, 595
BstMAI GTCTC 3 cut(s) 345, 604, 633
BstMBI GATC 2 cut(s) 202, 592
BstMWI GCNNNNNNNGC 1 cut(s) 468
BstNI CCWGG 4 cut(s) 399, 428, 464, 604
BstPAI GACNNNNGTC 1 cut(s) 30
BstPI GGTNACC 1 cut(s) 422
BstSCI CCNGG 4 cut(s) 397, 426, 462, 602
BstSFI CTRYAG 2 cut(s) 280, 344
BstSLI GKGCMC 1 cut(s) 585
BtsCI GGATG 1 cut(s) 238
BtsIMutI CAGTG 3 cut(s) 225, 464, 629
Cac8I GCNNGC 2 cut(s) 570, 610
Cfr13I GGNCC 2 cut(s) 32, 512
Csp6I GTAC 2 cut(s) 184, 550
CviAII CATG 3 cut(s) 109, 319, 392
CviJI RGCY 6 cut(s) 302, 471, 568, 572, 608, 647
CviKI_1 RGCY 6 cut(s) 302, 471, 568, 572, 608, 647
CviQI GTAC 2 cut(s) 184, 550
DdeI CTNAG 1 cut(s) 314
DpnI GATC 2 cut(s) 204, 594
DpnII GATC 2 cut(s) 202, 592
Eco130I CCWWGG 1 cut(s) 545
Eco31I GGTCTC 2 cut(s) 345, 633
Eco47I GGWCC 2 cut(s) 32, 512
Eco91I GGTNACC 1 cut(s) 422
EcoO65I GGTNACC 1 cut(s) 422
EcoRI GAATTC 2 cut(s) 99, 309
EcoRII CCWGG 4 cut(s) 397, 426, 462, 602
EcoT14I CCWWGG 1 cut(s) 545
ErhI CCWWGG 1 cut(s) 545
FaeI CATG 3 cut(s) 112, 322, 395
FaiI YATR 8 cut(s) 110, 132, 287, 289, 320, 393, 517, 519
FaqI GGGAC 1 cut(s) 38
FatI CATG 3 cut(s) 108, 318, 391
FauNDI CATATG 1 cut(s) 517
FbaI TGATCA 1 cut(s) 202
FblI GTMKAC 1 cut(s) 5
FokI GGATG 1 cut(s) 225
FspBI CTAG 1 cut(s) 489
HapII CCGG 1 cut(s) 30
Hin1II CATG 3 cut(s) 112, 322, 395
HincII GTYRAC 3 cut(s) 6, 67, 447
HindII GTYRAC 3 cut(s) 6, 67, 447
HinfI GANTC 6 cut(s) 18, 26, 163, 262, 541, 561
HpaII CCGG 1 cut(s) 30
HphI GGTGA 1 cut(s) 212
Hpy166II GTNNAC 5 cut(s) 6, 67, 447, 552, 583
Hpy188I TCNGA 2 cut(s) 17, 83
Hpy188III TCNNGA 4 cut(s) 271, 365, 452, 630
Hpy8I GTNNAC 5 cut(s) 6, 67, 447, 552, 583
HpyAV CCTTC 1 cut(s) 252
HpyCH4III ACNGT 5 cut(s) 10, 40, 58, 284, 624
HpyCH4IV ACGT 1 cut(s) 579
HpyCH4V TGCA 1 cut(s) 583
HpyF10VI GCNNNNNNNGC 1 cut(s) 468
HpyF3I CTNAG 1 cut(s) 314
HpySE526I ACGT 1 cut(s) 579
Hsp92II CATG 3 cut(s) 112, 322, 395
Ksp22I TGATCA 1 cut(s) 202
Kzo9I GATC 2 cut(s) 202, 592
MaeI CTAG 1 cut(s) 489
MaeII ACGT 1 cut(s) 579
MaeIII GTNAC 2 cut(s) 40, 422
MalI GATC 2 cut(s) 204, 594
MboI GATC 2 cut(s) 202, 592
MboII GAAGA 1 cut(s) 395
MhlI GDGCHC 1 cut(s) 585
MluCI AATT 5 cut(s) 99, 309, 432, 649, 654
MlyI GAGTC 2 cut(s) 20, 172
MmeI TCCRAC 1 cut(s) 59
MnlI CCTC 7 cut(s) 106, 133, 141, 246, 412, 503, 596
MseI TTAA 1 cut(s) 658
MslI CAYNNNNRTG 1 cut(s) 588
MspI CCGG 1 cut(s) 30
MspR9I CCNGG 4 cut(s) 399, 428, 464, 604
Mva1269I GAATGC 1 cut(s) 227
MvaI CCWGG 4 cut(s) 399, 428, 464, 604
MwoI GCNNNNNNNGC 1 cut(s) 468
NdeI CATATG 1 cut(s) 517
NdeII GATC 2 cut(s) 202, 592
NlaIII CATG 3 cut(s) 112, 322, 395
NlaIV GGNNCC 1 cut(s) 404
NspV TTCGAA 1 cut(s) 231
PctI GAATGC 1 cut(s) 227
PfeI GAWTC 4 cut(s) 18, 262, 541, 561
PleI GAGTC 2 cut(s) 20, 171
PpsI GAGTC 2 cut(s) 20, 171
Ppu21I YACGTR 1 cut(s) 580
PshAI GACNNNNGTC 1 cut(s) 30
Psp6I CCWGG 4 cut(s) 397, 426, 462, 602
PspEI GGTNACC 1 cut(s) 422
PspGI CCWGG 4 cut(s) 397, 426, 462, 602
PspN4I GGNNCC 1 cut(s) 404
PspPI GGNCC 2 cut(s) 32, 512
RsaI GTAC 2 cut(s) 185, 551
RsaNI GTAC 2 cut(s) 184, 550
RseI CAYNNNNRTG 1 cut(s) 588
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 658
Sau3AI GATC 2 cut(s) 202, 592
Sau96I GGNCC 2 cut(s) 32, 512
SchI GAGTC 2 cut(s) 20, 172
ScrFI CCNGG 4 cut(s) 399, 428, 464, 604
SduI GDGCHC 1 cut(s) 585
SetI ASST 8 cut(s) 202, 238, 281, 400, 440, 551, 582, 588
SfcI CTRYAG 2 cut(s) 280, 344
SfuI TTCGAA 1 cut(s) 231
SinI GGWCC 2 cut(s) 32, 512
SmiMI CAYNNNNRTG 1 cut(s) 588
SmlI CTYRAG 1 cut(s) 450
SmoI CTYRAG 1 cut(s) 450
Sse9I AATT 5 cut(s) 99, 309, 432, 649, 654
SsiI CCGC 1 cut(s) 406
SspMI CTAG 1 cut(s) 489
StyD4I CCNGG 4 cut(s) 397, 426, 462, 602
StyI CCWWGG 1 cut(s) 545
TaaI ACNGT 5 cut(s) 10, 40, 58, 284, 624
TaiI ACGT 1 cut(s) 582
TaqI TCGA 4 cut(s) 5, 231, 376, 631
TasI AATT 5 cut(s) 99, 309, 432, 649, 654
TfiI GAWTC 4 cut(s) 18, 262, 541, 561
Tru1I TTAA 1 cut(s) 658
Tru9I TTAA 1 cut(s) 658
TscAI CASTG 3 cut(s) 225, 464, 629
TspDTI ATGAA 3 cut(s) 402, 408, 633
TspGWI ACGGA 2 cut(s) 306, 487
TspRI CASTG 3 cut(s) 225, 464, 629
VneI GTGCAC 1 cut(s) 581
VpaK11BI GGWCC 2 cut(s) 32, 512
XapI RAATTY 2 cut(s) 99, 309
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.