Rh2AG274000

UPF0235 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0022686
Physical Location & Seq
Forward (+)
32643910 .. 32648631
4722 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG274000.1

Sequence Viewer

Length: 456 bp
ATGGTTTTCTTCCATTCTTTGGTAGCGGCGTTGCTGGTATTGCGAGCCGGCCCGGGCAGATCTGGTGTGGGCTGGGAGATGGCTTCAGGGCGACGTGATCTGGTTGGGGCTGTCCTTAGTTGCCGTGGTGGTTCTGTCTGGTGGGCTACCTTGGAGATGGTGGAGGTGGATGACTCTTGGCTTATGGAGGATGCGCAGAGACCGACCGGAAAATCTGGAAAGAAAGGAAGTGACAGACAATTTTCACCGGAAAAGCGCAGGCAACTTTCCGACTCCTCATCAGCGCTTATCGGCCACCGCACTTCGGCGGCGCGTGAACTGGTTTTAGGTGTAACAAGAAGGCAGGTTTCTATTGGATGTGGCTCTAAATCAAGAGACAAGGTTGTTATTGTAGAGGAGGTGACTCTACAAAGTGTTTTTGACATTCCGGATAGAGCTTCAAAGTGCAGCGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF167

Protein Analysis

151

Amino Acids

16.36

Weight (kDa)

9.5

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022686)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0118341
rosa_roxburghii Rroxscaffold_2G00124950 Rroxscaffold_2G00125620
rosa_samantha Rh2AG274000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 195
Acc36I ACCTGC 1 cut(s) 334
AccB7I CCANNNNNTGG 1 cut(s) 19
AccII CGCG 1 cut(s) 313
AccIII TCCGGA 1 cut(s) 427
AciI CCGC 3 cut(s) 26, 298, 308
AcoI YGGCCR 1 cut(s) 292
AcuI CTGAAG 1 cut(s) 69
AfeI AGCGCT 1 cut(s) 285
AfiI CCNNNNNNNGG 2 cut(s) 19, 304
AgsI TTSAA 1 cut(s) 441
AjiI CACGTC 1 cut(s) 95
AluBI AGCT 1 cut(s) 437
AluI AGCT 1 cut(s) 437
Alw26I GTCTC 2 cut(s) 193, 369
Ama87I CYCGRG 1 cut(s) 52
Aor13HI TCCGGA 1 cut(s) 427
Aor51HI AGCGCT 1 cut(s) 285
AoxI GGCC 2 cut(s) 49, 292
ApeKI GCWGC 1 cut(s) 447
AspLEI GCGC 5 cut(s) 196, 258, 286, 313, 452
AspS9I GGNCC 1 cut(s) 50
AsuC2I CCSGG 2 cut(s) 53, 54
AsuHPI GGTGA 2 cut(s) 237, 412
AvaI CYCGRG 1 cut(s) 52
BccI CCATC 2 cut(s) 73, 151
BceAI ACGGC 1 cut(s) 108
BcnI CCSGG 2 cut(s) 53, 54
BcoDI GTCTC 2 cut(s) 193, 369
BfoI RGCGCY 1 cut(s) 287
BfuAI ACCTGC 1 cut(s) 334
BglII AGATCT 1 cut(s) 59
BisI GCNGC 3 cut(s) 27, 309, 448
BlsI GCNGC 3 cut(s) 28, 310, 449
Bme1390I CCNGG 2 cut(s) 53, 54
BmeT110I CYCGRG 1 cut(s) 52
BmgBI CACGTC 1 cut(s) 95
BmgT120I GGNCC 1 cut(s) 50
BmrFI CCNGG 2 cut(s) 53, 54
BmsI GCATC 1 cut(s) 181
BpuMI CCSGG 2 cut(s) 53, 54
BsaI GGTCTC 1 cut(s) 193
BsaJI CCNNGG 3 cut(s) 52, 124, 150
BsaWI WCCGGW 3 cut(s) 206, 247, 427
Bsc4I CCNNNNNNNGG 2 cut(s) 19, 304
Bse118I RCCGGY 1 cut(s) 47
Bse1I ACTGG 1 cut(s) 324
BseAI TCCGGA 1 cut(s) 427
BseDI CCNNGG 3 cut(s) 52, 124, 150
BseGI GGATG 3 cut(s) 175, 196, 362
BseLI CCNNNNNNNGG 2 cut(s) 19, 304
BseNI ACTGG 1 cut(s) 324
BseRI GAGGAG 2 cut(s) 265, 410
BseYI CCCAGC 1 cut(s) 72
Bsh1236I CGCG 1 cut(s) 313
Bsh1285I CGRYCG 1 cut(s) 207
BshFI GGCC 2 cut(s) 51, 294
BsiEI CGRYCG 1 cut(s) 207
BsiHKCI CYCGRG 1 cut(s) 52
BsiSI CCGG 5 cut(s) 48, 53, 207, 248, 428
BslI CCNNNNNNNGG 2 cut(s) 19, 304
BsmAI GTCTC 2 cut(s) 193, 369
BsnI GGCC 2 cut(s) 51, 294
Bso31I GGTCTC 1 cut(s) 193
BsoBI CYCGRG 1 cut(s) 52
Bsp13I TCCGGA 1 cut(s) 427
Bsp143I GATC 2 cut(s) 59, 97
BspACI CCGC 3 cut(s) 26, 298, 308
BspANI GGCC 2 cut(s) 51, 294
BspEI TCCGGA 1 cut(s) 427
BspFNI CGCG 1 cut(s) 313
BspMI ACCTGC 1 cut(s) 334
BspTNI GGTCTC 1 cut(s) 193
BsrFI RCCGGY 1 cut(s) 47
BsrI ACTGG 1 cut(s) 324
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 3 cut(s) 52, 124, 150
BssMI GATC 2 cut(s) 59, 97
BssT1I CCWWGG 1 cut(s) 150
BstC8I GCNNGC 3 cut(s) 45, 49, 260
BstDEI CTNAG 1 cut(s) 116
BstDSI CCRYGG 1 cut(s) 124
BstF5I GGATG 3 cut(s) 175, 196, 362
BstFNI CGCG 1 cut(s) 313
BstH2I RGCGCY 1 cut(s) 287
BstHHI GCGC 5 cut(s) 196, 258, 286, 313, 452
BstKTI GATC 2 cut(s) 62, 100
BstMAI GTCTC 2 cut(s) 193, 369
BstMBI GATC 2 cut(s) 59, 97
BstMCI CGRYCG 1 cut(s) 207
BstMWI GCNNNNNNNGC 1 cut(s) 40
BstSCI CCNGG 2 cut(s) 51, 52
BstUI CGCG 1 cut(s) 313
BstX2I RGATCY 1 cut(s) 59
BstYI RGATCY 1 cut(s) 59
BsuRI GGCC 2 cut(s) 51, 294
BtgI CCRYGG 1 cut(s) 124
BtrI CACGTC 1 cut(s) 95
BtsCI GGATG 3 cut(s) 175, 196, 362
BveI ACCTGC 1 cut(s) 334
Cac8I GCNNGC 3 cut(s) 45, 49, 260
CfoI GCGC 5 cut(s) 196, 258, 286, 313, 452
Cfr10I RCCGGY 1 cut(s) 47
Cfr13I GGNCC 1 cut(s) 50
Cfr9I CCCGGG 1 cut(s) 52
DdeI CTNAG 1 cut(s) 116
DpnI GATC 2 cut(s) 61, 99
DpnII GATC 2 cut(s) 59, 97
EaeI YGGCCR 1 cut(s) 292
Eco130I CCWWGG 1 cut(s) 150
Eco31I GGTCTC 1 cut(s) 193
Eco47III AGCGCT 1 cut(s) 285
Eco57I CTGAAG 1 cut(s) 69
Eco88I CYCGRG 1 cut(s) 52
EcoT14I CCWWGG 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 150
FaiI YATR 2 cut(s) 185, 454
Fnu4HI GCNGC 3 cut(s) 27, 309, 448
FokI GGATG 3 cut(s) 182, 203, 369
Fsp4HI GCNGC 3 cut(s) 27, 309, 448
FspI TGCGCA 1 cut(s) 195
GlaI GCGC 5 cut(s) 195, 257, 285, 312, 451
GluI GCNGC 3 cut(s) 27, 309, 448
GsaI CCCAGC 1 cut(s) 76
HaeII RGCGCY 1 cut(s) 287
HaeIII GGCC 2 cut(s) 51, 294
HapII CCGG 5 cut(s) 48, 53, 207, 248, 428
HhaI GCGC 5 cut(s) 196, 258, 286, 313, 452
Hin6I GCGC 5 cut(s) 194, 256, 284, 311, 450
HinP1I GCGC 5 cut(s) 194, 256, 284, 311, 450
HinfI GANTC 3 cut(s) 173, 272, 403
HpaII CCGG 5 cut(s) 48, 53, 207, 248, 428
HphI GGTGA 2 cut(s) 237, 412
Hpy166II GTNNAC 1 cut(s) 317
Hpy188I TCNGA 1 cut(s) 271
Hpy188III TCNNGA 3 cut(s) 216, 372, 428
Hpy8I GTNNAC 1 cut(s) 317
Hpy99I CGWCG 1 cut(s) 96
HpyAV CCTTC 1 cut(s) 333
HpyCH4IV ACGT 1 cut(s) 94
HpyCH4V TGCA 1 cut(s) 447
HpyF10VI GCNNNNNNNGC 1 cut(s) 40
HpyF3I CTNAG 1 cut(s) 116
HpySE526I ACGT 1 cut(s) 94
HspAI GCGC 5 cut(s) 194, 256, 284, 311, 450
Kpn2I TCCGGA 1 cut(s) 427
KroI GCCGGC 1 cut(s) 47
KroNI GCCGGC 1 cut(s) 49
Kzo9I GATC 2 cut(s) 59, 97
LweI GCATC 1 cut(s) 181
MaeII ACGT 1 cut(s) 94
MaeIII GTNAC 3 cut(s) 230, 331, 400
MalI GATC 2 cut(s) 61, 99
MboI GATC 2 cut(s) 59, 97
MflI RGATCY 1 cut(s) 59
MluCI AATT 1 cut(s) 239
MlyI GAGTC 3 cut(s) 167, 266, 397
MmeI TCCRAC 1 cut(s) 294
MnlI CCTC 5 cut(s) 157, 181, 286, 388, 391
MroI TCCGGA 1 cut(s) 427
MroNI GCCGGC 1 cut(s) 47
MspI CCGG 5 cut(s) 48, 53, 207, 248, 428
MspR9I CCNGG 2 cut(s) 53, 54
MvnI CGCG 1 cut(s) 313
MwoI GCNNNNNNNGC 1 cut(s) 40
NaeI GCCGGC 1 cut(s) 49
NciI CCSGG 2 cut(s) 53, 54
NdeII GATC 2 cut(s) 59, 97
NgoMIV GCCGGC 1 cut(s) 47
NmuCI GTSAC 2 cut(s) 230, 400
NsbI TGCGCA 1 cut(s) 195
PdiI GCCGGC 1 cut(s) 49
PflMI CCANNNNNTGG 1 cut(s) 19
PkrI GCNGC 3 cut(s) 28, 310, 449
PleI GAGTC 3 cut(s) 167, 266, 397
PpsI GAGTC 3 cut(s) 167, 266, 397
PspFI CCCAGC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 50
PsuI RGATCY 1 cut(s) 59
SatI GCNGC 3 cut(s) 27, 309, 448
Sau3AI GATC 2 cut(s) 59, 97
Sau96I GGNCC 1 cut(s) 50
SchI GAGTC 3 cut(s) 167, 266, 397
ScrFI CCNGG 2 cut(s) 53, 54
SetI ASST 8 cut(s) 97, 152, 168, 331, 348, 384, 402, 439
SfaNI GCATC 1 cut(s) 181
SmaI CCCGGG 1 cut(s) 54
SrfI GCCCGGGC 1 cut(s) 54
Sse9I AATT 1 cut(s) 239
SsiI CCGC 3 cut(s) 26, 298, 308
StyD4I CCNGG 2 cut(s) 51, 52
StyI CCWWGG 1 cut(s) 150
TaiI ACGT 1 cut(s) 97
TaqII GACCGA 1 cut(s) 217
TasI AATT 1 cut(s) 239
TauI GCSGC 2 cut(s) 29, 311
TseFI GTSAC 2 cut(s) 230, 400
TseI GCWGC 1 cut(s) 447
Tsp45I GTSAC 2 cut(s) 230, 400
TspMI CCCGGG 1 cut(s) 52
Van91I CCANNNNNTGG 1 cut(s) 19
XmaI CCCGGG 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.