Rh2AG368300

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0031158
Physical Location & Seq
Reverse (-)
54629194 .. 54629940
747 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG368300.1

Sequence Viewer

Length: 726 bp
ATGACAGTACAGAGTCATAAGATAATGGTTTCCCGAACAAAAATGGCATCTGAGCTCTTTAGAGTTTCTACATTTTTATATACAGTATACACCAACACCAGCACATTTTTTTTTTGGATGAACACCAAGCGCTTACTGTTTTATAACGTAATTTATTTTCTCCCTCTTCATCGCTTTCCTCCTAAAATCTCCGGATCCATCTCTTCTCTTTCCCTCGAAACCCTCGGCCAAACTCAACGGTACTATACTACCATTCTACCGCCAGGACCCACCTCGATTCCCATAATTGGAAACTTCACATGGCTCTTCAAACTGACCTCCAGAATCAAACCCATCCTTCTTGACCTCCACGCCAATTACGGCCCCATCGACTCTCTCCCCATCCCCTTCAGTTCTCACCCCGCCATTTTCATCGACGACCACTCACAGACTCACTCGCCCACCAAGTCTTGGTCCAAAACGGCGCAGTCTGTTCGGACCGCCCTCCACCCCAGCCCATCACCAAGCTCATCATTAGCGACAAGCCGACAATCGTGTCATCTTCAGCAGCGGCTACGGCCCAACATGGCGCTTCCTCCGCCGGAACCTTATCTCCGAGGTTCTAAACCCTTCTCGGCTCAGGTCCTACGCTTCTTCCCGCAAATGGGCTCTTTGGACATCCTCACCAACCGTCTCGATCAGACGATCAGTCCGTATCGTCCAACGGACCAGTCGTCGAAGTTTTAG

Protein Analysis

241

Amino Acids

27.43

Weight (kDa)

10.22

Isoelectric Point (pI)

59.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031158)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0001782.1_g000009
rosa_samantha Rh2AG368300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 144
AasI GACNNNNNNGTC 1 cut(s) 534
AccB7I CCANNNNNTGG 1 cut(s) 450
AccI GTMKAC 1 cut(s) 87
AccIII TCCGGA 1 cut(s) 191
AciI CCGC 6 cut(s) 260, 402, 480, 550, 578, 638
AclWI GGATC 2 cut(s) 189, 202
AcoI YGGCCR 1 cut(s) 226
AcuI CTGAAG 2 cut(s) 373, 527
AfaI GTAC 2 cut(s) 9, 242
AfeI AGCGCT 1 cut(s) 131
AfiI CCNNNNNNNGG 4 cut(s) 287, 450, 643, 644
AgsI TTSAA 1 cut(s) 310
AhdI GACNNNNNGTC 2 cut(s) 687, 712
AjnI CCWGG 1 cut(s) 262
AloI GAACNNNNNNTCC 2 cut(s) 576, 608
AluBI AGCT 2 cut(s) 55, 507
AluI AGCT 2 cut(s) 55, 507
Alw21I GWGCWC 1 cut(s) 57
Alw26I GTCTC 1 cut(s) 677
AlwI GGATC 2 cut(s) 189, 202
Aor13HI TCCGGA 1 cut(s) 191
Aor51HI AGCGCT 1 cut(s) 131
AoxI GGCC 3 cut(s) 226, 361, 557
ApeKI GCWGC 1 cut(s) 547
AspLEI GCGC 3 cut(s) 132, 466, 571
AspS9I GGNCC 7 cut(s) 266, 362, 453, 477, 558, 622, 706
AsuHPI GGTGA 3 cut(s) 389, 492, 655
AvaII GGWCC 5 cut(s) 266, 453, 477, 622, 706
BamHI GGATCC 1 cut(s) 194
BanII GRGCYC 2 cut(s) 57, 650
Bbv12I GWGCWC 1 cut(s) 57
BbvI GCAGC 1 cut(s) 559
BccI CCATC 5 cut(s) 206, 341, 374, 389, 505
BceAI ACGGC 3 cut(s) 376, 477, 572
BcgI CGANNNNNNTGC 2 cut(s) 455, 489
BciT130I CCWGG 1 cut(s) 264
BcoDI GTCTC 1 cut(s) 677
BfoI RGCGCY 2 cut(s) 133, 572
BisI GCNGC 2 cut(s) 548, 551
BlsI GCNGC 2 cut(s) 549, 552
Bme1390I CCNGG 1 cut(s) 264
Bme18I GGWCC 5 cut(s) 266, 453, 477, 622, 706
BmeRI GACNNNNNGTC 2 cut(s) 687, 712
BmgT120I GGNCC 7 cut(s) 266, 362, 453, 477, 558, 622, 706
BmiI GGNNCC 4 cut(s) 196, 268, 364, 585
BmrFI CCNGG 1 cut(s) 264
BmsI GCATC 1 cut(s) 56
BpmI CTGGAG 1 cut(s) 304
Bpu10I CCTNAGC 1 cut(s) 618
BsaJI CCNNGG 2 cut(s) 223, 595
BsaWI WCCGGW 1 cut(s) 191
BsaXI ACNNNNNCTCC 2 cut(s) 576, 606
Bsc4I CCNNNNNNNGG 4 cut(s) 287, 450, 643, 644
Bse1I ACTGG 1 cut(s) 709
BseAI TCCGGA 1 cut(s) 191
BseBI CCWGG 1 cut(s) 264
BseDI CCNNGG 2 cut(s) 223, 595
BseGI GGATG 4 cut(s) 123, 333, 381, 657
BseLI CCNNNNNNNGG 4 cut(s) 287, 450, 643, 644
BseMII CTCAG 2 cut(s) 42, 632
BseNI ACTGG 1 cut(s) 709
BseXI GCAGC 1 cut(s) 559
BseYI CCCAGC 1 cut(s) 491
BshFI GGCC 3 cut(s) 228, 363, 559
BsiHKAI GWGCWC 1 cut(s) 57
BsiSI CCGG 2 cut(s) 192, 581
BslI CCNNNNNNNGG 4 cut(s) 287, 450, 643, 644
BsmAI GTCTC 1 cut(s) 677
BsmBI CGTCTC 1 cut(s) 677
BsnI GGCC 3 cut(s) 228, 363, 559
Bsp1286I GDGCHC 2 cut(s) 57, 650
Bsp13I TCCGGA 1 cut(s) 191
Bsp143I GATC 3 cut(s) 194, 676, 684
BspACI CCGC 6 cut(s) 260, 402, 480, 550, 578, 638
BspANI GGCC 3 cut(s) 228, 363, 559
BspCNI CTCAG 2 cut(s) 43, 631
BspEI TCCGGA 1 cut(s) 191
BspLI GGNNCC 4 cut(s) 196, 268, 364, 585
BspPI GGATC 2 cut(s) 189, 202
BspQI GCTCTTC 1 cut(s) 311
BsrI ACTGG 1 cut(s) 709
BssECI CCNNGG 2 cut(s) 223, 595
BssMI GATC 3 cut(s) 194, 676, 684
BssNAI GTATAC 1 cut(s) 88
Bst1107I GTATAC 1 cut(s) 88
Bst2UI CCWGG 1 cut(s) 264
Bst4CI ACNGT 5 cut(s) 7, 85, 138, 240, 671
Bst6I CTCTTC 3 cut(s) 171, 208, 311
BstDEI CTNAG 2 cut(s) 51, 618
BstF5I GGATG 4 cut(s) 123, 333, 381, 657
BstH2I RGCGCY 2 cut(s) 133, 572
BstHHI GCGC 3 cut(s) 132, 466, 571
BstKTI GATC 3 cut(s) 197, 679, 687
BstMAI GTCTC 1 cut(s) 677
BstMBI GATC 3 cut(s) 194, 676, 684
BstMWI GCNNNNNNNGC 2 cut(s) 556, 577
BstNI CCWGG 1 cut(s) 264
BstSCI CCNGG 1 cut(s) 262
BstV1I GCAGC 1 cut(s) 559
BstX2I RGATCY 1 cut(s) 194
BstYI RGATCY 1 cut(s) 194
BstZ17I GTATAC 1 cut(s) 88
BsuRI GGCC 3 cut(s) 228, 363, 559
BtgZI GCGATG 1 cut(s) 155
BtsCI GGATG 4 cut(s) 123, 333, 381, 657
CfoI GCGC 3 cut(s) 132, 466, 571
Cfr13I GGNCC 7 cut(s) 266, 362, 453, 477, 558, 622, 706
CpoI CGGWCCG 1 cut(s) 477
Csp6I GTAC 2 cut(s) 8, 241
CspI CGGWCCG 1 cut(s) 477
CviAII CATG 2 cut(s) 300, 565
CviQI GTAC 2 cut(s) 8, 241
DdeI CTNAG 2 cut(s) 51, 618
DpnI GATC 3 cut(s) 196, 678, 686
DpnII GATC 3 cut(s) 194, 676, 684
DrdI GACNNNNNNGTC 1 cut(s) 534
DriI GACNNNNNGTC 2 cut(s) 687, 712
DseDI GACNNNNNNGTC 1 cut(s) 534
EaeI YGGCCR 1 cut(s) 226
Eam1104I CTCTTC 3 cut(s) 171, 208, 311
Eam1105I GACNNNNNGTC 2 cut(s) 687, 712
EarI CTCTTC 3 cut(s) 171, 208, 311
EciI GGCGGA 1 cut(s) 567
Ecl136II GAGCTC 1 cut(s) 55
Eco24I GRGCYC 2 cut(s) 57, 650
Eco47I GGWCC 5 cut(s) 266, 453, 477, 622, 706
Eco47III AGCGCT 1 cut(s) 131
Eco53kI GAGCTC 1 cut(s) 55
Eco57I CTGAAG 2 cut(s) 373, 527
EcoICRI GAGCTC 1 cut(s) 55
EcoO109I RGGNCCY 2 cut(s) 266, 622
EcoRII CCWGG 1 cut(s) 262
EcoT38I GRGCYC 2 cut(s) 57, 650
Esp3I CGTCTC 1 cut(s) 677
FaeI CATG 2 cut(s) 303, 568
FaiI YATR 9 cut(s) 18, 79, 81, 88, 144, 246, 284, 301, 566
FatI CATG 2 cut(s) 299, 564
FauI CCCGC 2 cut(s) 409, 645
FblI GTMKAC 1 cut(s) 87
Fnu4HI GCNGC 2 cut(s) 548, 551
FokI GGATG 4 cut(s) 130, 320, 368, 644
FriOI GRGCYC 2 cut(s) 57, 650
Fsp4HI GCNGC 2 cut(s) 548, 551
GlaI GCGC 3 cut(s) 131, 465, 570
GluI GCNGC 2 cut(s) 548, 551
GsaI CCCAGC 1 cut(s) 495
GsuI CTGGAG 1 cut(s) 304
HaeII RGCGCY 2 cut(s) 133, 572
HaeIII GGCC 3 cut(s) 228, 363, 559
HapII CCGG 2 cut(s) 192, 581
HhaI GCGC 3 cut(s) 132, 466, 571
Hin1II CATG 2 cut(s) 303, 568
Hin6I GCGC 3 cut(s) 130, 464, 569
HinP1I GCGC 3 cut(s) 130, 464, 569
HinfI GANTC 5 cut(s) 13, 277, 324, 371, 430
HpaII CCGG 2 cut(s) 192, 581
HphI GGTGA 3 cut(s) 389, 492, 655
Hpy166II GTNNAC 1 cut(s) 88
Hpy188I TCNGA 4 cut(s) 52, 477, 596, 681
Hpy188III TCNNGA 5 cut(s) 33, 192, 321, 341, 674
Hpy8I GTNNAC 1 cut(s) 88
Hpy99I CGWCG 2 cut(s) 419, 718
HpyAV CCTTC 3 cut(s) 347, 397, 619
HpyCH4III ACNGT 5 cut(s) 7, 85, 138, 240, 671
HpyCH4IV ACGT 1 cut(s) 147
HpyF10VI GCNNNNNNNGC 2 cut(s) 556, 577
HpyF3I CTNAG 2 cut(s) 51, 618
HpySE526I ACGT 1 cut(s) 147
Hsp92II CATG 2 cut(s) 303, 568
HspAI GCGC 3 cut(s) 130, 464, 569
Kpn2I TCCGGA 1 cut(s) 191
Kzo9I GATC 3 cut(s) 194, 676, 684
LguI GCTCTTC 1 cut(s) 311
LpnPI CCDG 9 cut(s) 112, 205, 249, 276, 334, 505, 594, 605, 722
Lsp1109I GCAGC 1 cut(s) 559
LweI GCATC 1 cut(s) 56
MaeII ACGT 1 cut(s) 147
MalI GATC 3 cut(s) 196, 678, 686
MboI GATC 3 cut(s) 194, 676, 684
MboII GAAGA 5 cut(s) 158, 195, 298, 533, 625
MflI RGATCY 1 cut(s) 194
MhlI GDGCHC 2 cut(s) 57, 650
MluCI AATT 3 cut(s) 150, 285, 355
MlyI GAGTC 3 cut(s) 22, 365, 424
MmeI TCCRAC 1 cut(s) 725
MroI TCCGGA 1 cut(s) 191
MspA1I CMGCKG 1 cut(s) 550
MspI CCGG 2 cut(s) 192, 581
MspR9I CCNGG 1 cut(s) 264
MvaI CCWGG 1 cut(s) 264
MwoI GCNNNNNNNGC 2 cut(s) 556, 577
NdeII GATC 3 cut(s) 194, 676, 684
NlaIII CATG 2 cut(s) 303, 568
NlaIV GGNNCC 4 cut(s) 196, 268, 364, 585
NmeAIII GCCGAG 2 cut(s) 204, 593
PciSI GCTCTTC 1 cut(s) 311
PcsI WCGNNNNNNNCGW 3 cut(s) 366, 689, 710
PfeI GAWTC 2 cut(s) 277, 324
PflMI CCANNNNNTGG 1 cut(s) 450
PkrI GCNGC 2 cut(s) 549, 552
PleI GAGTC 3 cut(s) 21, 365, 424
PpsI GAGTC 3 cut(s) 21, 365, 424
PpuMI RGGWCCY 2 cut(s) 266, 622
PsiI TTATAA 1 cut(s) 144
Psp124BI GAGCTC 1 cut(s) 57
Psp5II RGGWCCY 2 cut(s) 266, 622
Psp6I CCWGG 1 cut(s) 262
PspFI CCCAGC 1 cut(s) 491
PspGI CCWGG 1 cut(s) 262
PspN4I GGNNCC 4 cut(s) 196, 268, 364, 585
PspPI GGNCC 7 cut(s) 266, 362, 453, 477, 558, 622, 706
PspPPI RGGWCCY 2 cut(s) 266, 622
PsuI RGATCY 1 cut(s) 194
RsaI GTAC 2 cut(s) 9, 242
RsaNI GTAC 2 cut(s) 8, 241
Rsr2I CGGWCCG 1 cut(s) 477
RsrII CGGWCCG 1 cut(s) 477
SacI GAGCTC 1 cut(s) 57
SapI GCTCTTC 1 cut(s) 311
SatI GCNGC 2 cut(s) 548, 551
Sau3AI GATC 3 cut(s) 194, 676, 684
Sau96I GGNCC 7 cut(s) 266, 362, 453, 477, 558, 622, 706
SchI GAGTC 3 cut(s) 22, 365, 424
ScrFI CCNGG 1 cut(s) 264
SduI GDGCHC 2 cut(s) 57, 650
SetI ASST 9 cut(s) 57, 150, 275, 320, 348, 509, 589, 601, 624
SfaNI GCATC 1 cut(s) 56
SinI GGWCC 5 cut(s) 266, 453, 477, 622, 706
Sse9I AATT 3 cut(s) 150, 285, 355
SsiI CCGC 6 cut(s) 260, 402, 480, 550, 578, 638
SstI GAGCTC 1 cut(s) 57
StyD4I CCNGG 1 cut(s) 262
TaaI ACNGT 5 cut(s) 7, 85, 138, 240, 671
TaiI ACGT 1 cut(s) 150
TaqI TCGA 6 cut(s) 216, 275, 369, 414, 675, 716
TasI AATT 3 cut(s) 150, 285, 355
TatI WGTACW 1 cut(s) 7
TauI GCSGC 1 cut(s) 553
TfiI GAWTC 2 cut(s) 277, 324
TseI GCWGC 1 cut(s) 547
TspDTI ATGAA 3 cut(s) 134, 158, 400
TspGWI ACGGA 2 cut(s) 681, 719
Van91I CCANNNNNTGG 1 cut(s) 450
VpaK11BI GGWCC 5 cut(s) 266, 453, 477, 622, 706
XmiI GTMKAC 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.