Rh2AG424600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0025489
Physical Location & Seq
Forward (+)
63138950 .. 63143483
4534 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG424600.1

Sequence Viewer

Length: 540 bp
ATGAGCCCTCTCAATTTCAATGACCGTCGTGGCCATGATGATGATGACGCTGGCGGTGAGCGACAAGTTTTGGCTGGAGAGGATGAGGAAATTGGGGAAGAGGACATTTGCTCAAAGATTGCAAGGAGGTTTCCGGATTGGGTTTTCTCATGTGAAGCTTCGTCGCCAGCTTCGCCCTTCCACTTCATCTCCTGTTGCGGCGAAGAGACTCGGCATCAAAAGCTCGAGTCTGATTTGGAGGAGTTGGAGCGCACGGTGGAGTTGGTGGTGCCGAAGAAGATTGTTGAACGATTGGCCGCACAGTGTTTGTTTTCGACGGGGGAGGCCGGAGCCAACCTTGGTCACAGGGACAACGACGGCGGTACTGGATCAGAAGGCACCGTAGTTGGTGCATTGAACGCTCCTGATACCGGAAAGCGATGCAAATCAATTTTGGCACCACCACTAGAGGTAGAGAAAGTGGCTTTTAGAACCGGCCAGGACTCAGGTGTTCCCCACAGGCCGAGAACCAGTGTCTTCTATTCTATGGCTGCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

19.39

Weight (kDa)

4.84

Isoelectric Point (pI)

50.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025489)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000760.1_g000017
rosa_roxburghii Rroxscaffold_2G00110870
rosa_samantha Rh2AG424600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 268, 377, 436
AccIII TCCGGA 1 cut(s) 133
AciI CCGC 4 cut(s) 54, 198, 297, 360
AclWI GGATC 1 cut(s) 376
AcoI YGGCCR 3 cut(s) 31, 294, 475
AfaI GTAC 1 cut(s) 364
AfiI CCNNNNNNNGG 1 cut(s) 410
AgsI TTSAA 3 cut(s) 19, 287, 397
AjnI CCWGG 1 cut(s) 477
AluBI AGCT 3 cut(s) 158, 170, 223
AluI AGCT 3 cut(s) 158, 170, 223
Alw26I GTCTC 1 cut(s) 200
AlwI GGATC 1 cut(s) 376
Ama87I CYCGRG 1 cut(s) 224
Aor13HI TCCGGA 1 cut(s) 133
AoxI GGCC 5 cut(s) 31, 294, 324, 475, 500
ApeKI GCWGC 1 cut(s) 530
AspLEI GCGC 1 cut(s) 252
AsuHPI GGTGA 1 cut(s) 68
AvaI CYCGRG 1 cut(s) 224
BalI TGGCCA 1 cut(s) 33
BanI GGYRCC 3 cut(s) 268, 377, 436
BanII GRGCYC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 508
BbvI GCAGC 1 cut(s) 517
BceAI ACGGC 1 cut(s) 373
BciT130I CCWGG 1 cut(s) 479
BcoDI GTCTC 1 cut(s) 200
BfaI CTAG 1 cut(s) 446
BisI GCNGC 3 cut(s) 199, 297, 531
BlsI GCNGC 3 cut(s) 200, 298, 532
Bme1390I CCNGG 1 cut(s) 479
BmeT110I CYCGRG 1 cut(s) 224
BmiI GGNNCC 4 cut(s) 270, 331, 379, 438
BmrFI CCNGG 1 cut(s) 479
BmsI GCATC 2 cut(s) 223, 410
BpiI GAAGAC 1 cut(s) 508
BpmI CTGGAG 1 cut(s) 96
BsaBI GATNNNNATC 1 cut(s) 424
BsaJI CCNNGG 1 cut(s) 337
BsaWI WCCGGW 2 cut(s) 133, 410
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bsc4I CCNNNNNNNGG 1 cut(s) 410
Bse118I RCCGGY 1 cut(s) 473
Bse1I ACTGG 2 cut(s) 370, 510
Bse8I GATNNNNATC 1 cut(s) 424
BseAI TCCGGA 1 cut(s) 133
BseBI CCWGG 1 cut(s) 479
BseDI CCNNGG 1 cut(s) 337
BseGI GGATG 1 cut(s) 88
BseJI GATNNNNATC 1 cut(s) 424
BseLI CCNNNNNNNGG 1 cut(s) 410
BseMII CTCAG 1 cut(s) 498
BseNI ACTGG 2 cut(s) 370, 510
BseRI GAGGAG 1 cut(s) 254
BseXI GCAGC 1 cut(s) 517
BshFI GGCC 5 cut(s) 33, 296, 326, 477, 502
BshNI GGYRCC 3 cut(s) 268, 377, 436
BsiHKCI CYCGRG 1 cut(s) 224
BsiSI CCGG 4 cut(s) 134, 327, 411, 474
BslFI GGGAC 1 cut(s) 362
BslI CCNNNNNNNGG 1 cut(s) 410
BsmAI GTCTC 1 cut(s) 200
BsmFI GGGAC 1 cut(s) 362
BsnI GGCC 5 cut(s) 33, 296, 326, 477, 502
BsoBI CYCGRG 1 cut(s) 224
Bsp1286I GDGCHC 1 cut(s) 8
Bsp13I TCCGGA 1 cut(s) 133
Bsp143I GATC 1 cut(s) 368
BspACI CCGC 4 cut(s) 54, 198, 297, 360
BspANI GGCC 5 cut(s) 33, 296, 326, 477, 502
BspCNI CTCAG 1 cut(s) 497
BspEI TCCGGA 1 cut(s) 133
BspLI GGNNCC 4 cut(s) 270, 331, 379, 438
BspPI GGATC 1 cut(s) 376
BspT107I GGYRCC 3 cut(s) 268, 377, 436
BsrFI RCCGGY 1 cut(s) 473
BsrI ACTGG 2 cut(s) 370, 510
BssAI RCCGGY 1 cut(s) 473
BssECI CCNNGG 1 cut(s) 337
BssMI GATC 1 cut(s) 368
BssT1I CCWWGG 1 cut(s) 337
Bst2UI CCWGG 1 cut(s) 479
Bst4CI ACNGT 4 cut(s) 26, 256, 303, 382
Bst6I CTCTTC 2 cut(s) 93, 198
BstC8I GCNNGC 2 cut(s) 52, 168
BstDEI CTNAG 1 cut(s) 484
BstF5I GGATG 1 cut(s) 88
BstHHI GCGC 1 cut(s) 252
BstKTI GATC 1 cut(s) 371
BstMAI GTCTC 1 cut(s) 200
BstMBI GATC 1 cut(s) 368
BstMWI GCNNNNNNNGC 3 cut(s) 172, 220, 398
BstNI CCWGG 1 cut(s) 479
BstSCI CCNGG 1 cut(s) 477
BstV1I GCAGC 1 cut(s) 517
BstV2I GAAGAC 1 cut(s) 508
BsuRI GGCC 5 cut(s) 33, 296, 326, 477, 502
BtgZI GCGATG 1 cut(s) 433
BtsCI GGATG 1 cut(s) 88
BtsIMutI CAGTG 2 cut(s) 308, 517
Cac8I GCNNGC 2 cut(s) 52, 168
CfoI GCGC 1 cut(s) 252
Cfr10I RCCGGY 1 cut(s) 473
CseI GACGC 1 cut(s) 56
Csp6I GTAC 1 cut(s) 363
CviAII CATG 2 cut(s) 35, 150
CviQI GTAC 1 cut(s) 363
DdeI CTNAG 1 cut(s) 484
DpnI GATC 1 cut(s) 370
DpnII GATC 1 cut(s) 368
EaeI YGGCCR 3 cut(s) 31, 294, 475
Eam1104I CTCTTC 2 cut(s) 93, 198
EarI CTCTTC 2 cut(s) 93, 198
Eco130I CCWWGG 1 cut(s) 337
Eco24I GRGCYC 1 cut(s) 8
Eco88I CYCGRG 1 cut(s) 224
EcoRII CCWGG 1 cut(s) 477
EcoT14I CCWWGG 1 cut(s) 337
EcoT38I GRGCYC 1 cut(s) 8
ErhI CCWWGG 1 cut(s) 337
FaeI CATG 2 cut(s) 38, 153
FaiI YATR 3 cut(s) 36, 151, 527
FaqI GGGAC 1 cut(s) 362
FatI CATG 2 cut(s) 34, 149
Fnu4HI GCNGC 3 cut(s) 199, 297, 531
FokI GGATG 1 cut(s) 95
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 3 cut(s) 199, 297, 531
FspBI CTAG 1 cut(s) 446
GlaI GCGC 1 cut(s) 251
GluI GCNGC 3 cut(s) 199, 297, 531
GsuI CTGGAG 1 cut(s) 96
HaeIII GGCC 5 cut(s) 33, 296, 326, 477, 502
HapII CCGG 4 cut(s) 134, 327, 411, 474
HgaI GACGC 1 cut(s) 56
HhaI GCGC 1 cut(s) 252
Hin1II CATG 2 cut(s) 38, 153
Hin6I GCGC 1 cut(s) 250
HinP1I GCGC 1 cut(s) 250
HindIII AAGCTT 1 cut(s) 156
HinfI GANTC 3 cut(s) 208, 227, 482
HpaII CCGG 4 cut(s) 134, 327, 411, 474
HphI GGTGA 1 cut(s) 68
Hpy188I TCNGA 2 cut(s) 232, 373
Hpy188III TCNNGA 2 cut(s) 134, 404
Hpy99I CGWCG 4 cut(s) 30, 166, 319, 359
HpyAV CCTTC 2 cut(s) 187, 368
HpyCH4III ACNGT 4 cut(s) 26, 256, 303, 382
HpyCH4V TGCA 3 cut(s) 122, 392, 423
HpyF10VI GCNNNNNNNGC 3 cut(s) 172, 220, 398
HpyF3I CTNAG 1 cut(s) 484
Hsp92II CATG 2 cut(s) 38, 153
HspAI GCGC 1 cut(s) 250
Kpn2I TCCGGA 1 cut(s) 133
Kzo9I GATC 1 cut(s) 368
LmnI GCTCC 3 cut(s) 247, 329, 406
Lsp1109I GCAGC 1 cut(s) 517
LweI GCATC 2 cut(s) 223, 410
MaeI CTAG 1 cut(s) 446
MaeIII GTNAC 1 cut(s) 341
MalI GATC 1 cut(s) 370
MboI GATC 1 cut(s) 368
MboII GAAGA 5 cut(s) 110, 215, 286, 289, 508
MhlI GDGCHC 1 cut(s) 8
MlsI TGGCCA 1 cut(s) 33
MluCI AATT 3 cut(s) 13, 90, 429
MluNI TGGCCA 1 cut(s) 33
MlyI GAGTC 3 cut(s) 202, 236, 476
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 8 cut(s) 18, 73, 79, 94, 120, 232, 316, 442
Mox20I TGGCCA 1 cut(s) 33
MroI TCCGGA 1 cut(s) 133
MscI TGGCCA 1 cut(s) 33
MseI TTAA 1 cut(s) 538
MslI CAYNNNNRTG 1 cut(s) 39
Msp20I TGGCCA 1 cut(s) 33
MspI CCGG 4 cut(s) 134, 327, 411, 474
MspR9I CCNGG 1 cut(s) 479
MvaI CCWGG 1 cut(s) 479
MwoI GCNNNNNNNGC 3 cut(s) 172, 220, 398
NdeII GATC 1 cut(s) 368
NlaIII CATG 2 cut(s) 38, 153
NlaIV GGNNCC 4 cut(s) 270, 331, 379, 438
NmeAIII GCCGAG 2 cut(s) 190, 528
NmuCI GTSAC 1 cut(s) 341
PaeR7I CTCGAG 1 cut(s) 224
PkrI GCNGC 3 cut(s) 200, 298, 532
PleI GAGTC 3 cut(s) 202, 235, 476
PpsI GAGTC 3 cut(s) 202, 235, 476
Psp6I CCWGG 1 cut(s) 477
PspGI CCWGG 1 cut(s) 477
PspN4I GGNNCC 4 cut(s) 270, 331, 379, 438
PspXI VCTCGAGB 1 cut(s) 224
RsaI GTAC 1 cut(s) 364
RsaNI GTAC 1 cut(s) 363
RseI CAYNNNNRTG 1 cut(s) 39
SaqAI TTAA 1 cut(s) 538
SatI GCNGC 3 cut(s) 199, 297, 531
Sau3AI GATC 1 cut(s) 368
SchI GAGTC 3 cut(s) 202, 236, 476
ScrFI CCNGG 1 cut(s) 479
SduI GDGCHC 1 cut(s) 8
SetI ASST 7 cut(s) 131, 160, 172, 225, 339, 453, 490
SfaNI GCATC 2 cut(s) 223, 410
Sfr274I CTCGAG 1 cut(s) 224
SlaI CTCGAG 1 cut(s) 224
SmiMI CAYNNNNRTG 1 cut(s) 39
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
Sse9I AATT 3 cut(s) 13, 90, 429
SsiI CCGC 4 cut(s) 54, 198, 297, 360
SspMI CTAG 1 cut(s) 446
StyD4I CCNGG 1 cut(s) 477
StyI CCWWGG 1 cut(s) 337
TaaI ACNGT 4 cut(s) 26, 256, 303, 382
TaqI TCGA 2 cut(s) 225, 314
TasI AATT 3 cut(s) 13, 90, 429
TauI GCSGC 2 cut(s) 201, 299
Tru1I TTAA 1 cut(s) 538
Tru9I TTAA 1 cut(s) 538
TscAI CASTG 2 cut(s) 308, 517
TseFI GTSAC 1 cut(s) 341
TseI GCWGC 1 cut(s) 530
Tsp45I GTSAC 1 cut(s) 341
TspDTI ATGAA 1 cut(s) 175
TspRI CASTG 2 cut(s) 308, 517
XhoI CTCGAG 1 cut(s) 224
XspI CTAG 1 cut(s) 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.