Rh2AG536300

Cytosolic domain of 10TM putative phosphate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0031016
Physical Location & Seq
Forward (+)
76433385 .. 76437647
4263 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG536300.1

Sequence Viewer

Length: 1158 bp
ATGGCTACACTGGGTGATATAGGATTTGCAGCAGCAATCAATATTCTGACTGCATTTGCTTTCTTTGTGGCGTTTGCCATACTCCGGCTTCAACCTGTGAACGATAGGGTCTACTTTCCAAAATGGTATCTCAAAGGACTGAGGGCCAGTCCGTTGGGCGGGGCACTTGTAACCAAATTTGTGAATTTGGACTACAGGTCATATGTGAGGTTTCTGAATTGGATGCCTGCCGCATTGCAAATGCCGGAACCTGAGCTAGTTGAACATGCAGGGTTGGACTCTGTTGTTTACCTGAGGATTTACTTGACAGGGCTAAAGATTTTTGTTCCTATTGCATTTCTGGCGTTTTCAGTCATGGTCCCTGTCAATTGGACTAACAGCACCCTAAAGAAGTCAAATTTAGTTTACAGCAACCTAGATGAGCTTTCTATTTCAAATGTTCCTCTTGGATCTCATAGATTTTGGACTCATTTAGTAATGGCCTATGCTTTTACATTCTGGACATGCTATGTTTTGAATAGGGAGTATGAGATTGTTGCAACAATGCGATTGCACTTTCTTGCATCAGAACAACGGCGCCCTGATCAGTTCACAGTACTGGTTAGAAATGTGCCACCAGATCCTGATGAAACAGTCAGTCAGCTTGTGGAACATTTTTTTCTTGTTAACCACCCAGATCATTATCTCACTCATCAGGTTGTTTACAATGCAAATAAACTTTCTGAATTAGTCAATGAGAAGAAGAAAGTTCAGAATTGGCTCGACTACTATCAACTTAAATTTTCAAGAAATCCATCAAGAAGACCATTAACAAAGACTGGATTTCTTGGTCTTTTGGGTAAGAGGGTGGATGGAATTGATCATTATACATCTGAGGTTGAAAGGCTATCAAAAGAGGAAGCAATGAGGAAAGATACATTAGAACGTGCGAGGGAGCCTAATCTGAACTTAAAAGGCTTCCTTCAAAGTGCATACATTCACCCAGTTTTCAAGGTTGCAGATGACAGCGACAGTGATGGAGCTTCCGAAGAATTGGAGCAGGAGCCTGTCGTTGTCCCAACGAAACGCCAGTCTCGAAGGAACACACCCGTGCCCAGCAAATATAGTGGTTCAATATCATCGTCCCAAGCTGAAGATGTTCTCATGATCCAGCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHM7_cyt RSN1_7TM RSN1_TM

Protein Analysis

385

Amino Acids

44.09

Weight (kDa)

8.4

Isoelectric Point (pI)

47.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 9 - 175 8.2e-42 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 196 - 305 3.1e-26 Cytosolic domain of 10TM putative phosphate transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031016)

Species Orthologous Gene IDs
rosa_multiflora Rmu_ssc0000284.1_g000002
rosa_samantha Rh2AG536300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 576
AccI GTMKAC 1 cut(s) 111
AciI CCGC 2 cut(s) 159, 231
AclWI GGATC 3 cut(s) 457, 614, 1141
AcsI RAATTY 4 cut(s) 176, 184, 397, 779
AcuI CTGAAG 1 cut(s) 1152
AcyI GRCGYC 1 cut(s) 577
AdeI CACNNNGTG 1 cut(s) 14
AfaI GTAC 1 cut(s) 597
AfiI CCNNNNNNNGG 2 cut(s) 84, 158
AgsI TTSAA 9 cut(s) 92, 263, 435, 517, 786, 881, 965, 991, 1113
AhdI GACNNNNNGTC 1 cut(s) 196
AleI CACNNNNGTG 1 cut(s) 1088
AluBI AGCT 5 cut(s) 256, 424, 643, 1022, 1130
AluI AGCT 5 cut(s) 256, 424, 643, 1022, 1130
Alw26I GTCTC 1 cut(s) 1077
AlwI GGATC 3 cut(s) 457, 614, 1141
AlwNI CAGNNNCTG 1 cut(s) 623
AoxI GGCC 2 cut(s) 144, 480
ApeKI GCWGC 2 cut(s) 29, 32
ApoI RAATTY 4 cut(s) 176, 184, 397, 779
ArsI GACNNNNNNTTYG 2 cut(s) 1055, 1087
Asp700I GAANNNNTTC 1 cut(s) 1137
AspLEI GCGC 1 cut(s) 579
AspS9I GGNCC 2 cut(s) 144, 358
AsuHPI GGTGA 2 cut(s) 26, 971
AvaII GGWCC 1 cut(s) 358
AxyI CCTNAGG 1 cut(s) 293
BaeGI GKGCMC 2 cut(s) 166, 1095
BanI GGYRCC 1 cut(s) 576
BbsI GAAGAC 1 cut(s) 808
BbvI GCAGC 2 cut(s) 41, 44
BccI CCATC 3 cut(s) 802, 845, 1010
BceAI ACGGC 1 cut(s) 590
BclI TGATCA 2 cut(s) 583, 859
BcoDI GTCTC 1 cut(s) 1077
BfaI CTAG 2 cut(s) 257, 416
BfmI CTRYAG 1 cut(s) 193
BfoI RGCGCY 1 cut(s) 580
BisI GCNGC 3 cut(s) 30, 33, 231
BlsI GCNGC 3 cut(s) 31, 34, 232
BmcAI AGTACT 1 cut(s) 597
Bme18I GGWCC 1 cut(s) 358
BmeRI GACNNNNNGTC 1 cut(s) 196
BmgT120I GGNCC 2 cut(s) 144, 358
BmiI GGNNCC 5 cut(s) 249, 360, 578, 936, 1044
BmrI ACTGGG 2 cut(s) 20, 977
BmsI GCATC 2 cut(s) 213, 572
BmuI ACTGGG 2 cut(s) 20, 977
BpiI GAAGAC 1 cut(s) 808
Bpu10I CCTNAGC 1 cut(s) 252
BsaBI GATNNNNATC 1 cut(s) 681
BsaHI GRCGYC 1 cut(s) 577
Bsc4I CCNNNNNNNGG 2 cut(s) 84, 158
Bse1I ACTGG 6 cut(s) 15, 147, 603, 823, 983, 1069
Bse21I CCTNAGG 1 cut(s) 293
Bse3DI GCAATG 2 cut(s) 233, 909
Bse8I GATNNNNATC 1 cut(s) 681
BseGI GGATG 2 cut(s) 228, 856
BseJI GATNNNNATC 1 cut(s) 681
BseLI CCNNNNNNNGG 2 cut(s) 84, 158
BseMI GCAATG 2 cut(s) 233, 909
BseMII CTCAG 4 cut(s) 131, 243, 284, 864
BseNI ACTGG 6 cut(s) 15, 147, 603, 823, 983, 1069
BseSI GKGCMC 2 cut(s) 166, 1095
BseXI GCAGC 2 cut(s) 41, 44
BseYI CCCAGC 1 cut(s) 1094
BshFI GGCC 2 cut(s) 146, 482
BshNI GGYRCC 1 cut(s) 576
BsiSI CCGG 2 cut(s) 85, 245
BslFI GGGAC 3 cut(s) 344, 1040, 1108
BslI CCNNNNNNNGG 2 cut(s) 84, 158
BsmAI GTCTC 1 cut(s) 1077
BsmFI GGGAC 3 cut(s) 344, 1040, 1108
BsnI GGCC 2 cut(s) 146, 482
Bsp1286I GDGCHC 2 cut(s) 166, 1095
Bsp143I GATC 6 cut(s) 449, 583, 619, 676, 859, 1146
BspACI CCGC 2 cut(s) 159, 231
BspANI GGCC 2 cut(s) 146, 482
BspCNI CTCAG 4 cut(s) 132, 244, 285, 865
BspHI TCATGA 1 cut(s) 1143
BspLI GGNNCC 5 cut(s) 249, 360, 578, 936, 1044
BspPI GGATC 3 cut(s) 457, 614, 1141
BspT107I GGYRCC 1 cut(s) 576
BsrDI GCAATG 2 cut(s) 233, 909
BsrI ACTGG 6 cut(s) 15, 147, 603, 823, 983, 1069
BssMI GATC 6 cut(s) 449, 583, 619, 676, 859, 1146
BssNI GRCGYC 1 cut(s) 577
Bst4CI ACNGT 3 cut(s) 595, 634, 1013
BstACI GRCGYC 1 cut(s) 577
BstC8I GCNNGC 1 cut(s) 228
BstDEI CTNAG 5 cut(s) 140, 252, 293, 873, 1155
BstF5I GGATG 2 cut(s) 228, 856
BstH2I RGCGCY 1 cut(s) 580
BstHHI GCGC 1 cut(s) 579
BstKTI GATC 6 cut(s) 452, 586, 622, 679, 862, 1149
BstMAI GTCTC 1 cut(s) 1077
BstMBI GATC 6 cut(s) 449, 583, 619, 676, 859, 1146
BstMWI GCNNNNNNNGC 1 cut(s) 341
BstNSI RCATGY 2 cut(s) 269, 507
BstSFI CTRYAG 1 cut(s) 193
BstSLI GKGCMC 2 cut(s) 166, 1095
BstV1I GCAGC 2 cut(s) 41, 44
BstV2I GAAGAC 1 cut(s) 808
BstX2I RGATCY 2 cut(s) 449, 619
BstXI CCANNNNNNTGG 1 cut(s) 154
BstYI RGATCY 2 cut(s) 449, 619
Bsu36I CCTNAGG 1 cut(s) 293
BsuRI GGCC 2 cut(s) 146, 482
BtsCI GGATG 2 cut(s) 228, 856
BtsIMutI CAGTG 2 cut(s) 8, 1018
Cac8I GCNNGC 1 cut(s) 228
CaiI CAGNNNCTG 1 cut(s) 623
CciI TCATGA 1 cut(s) 1143
CfoI GCGC 1 cut(s) 579
Cfr13I GGNCC 2 cut(s) 144, 358
Csp6I GTAC 1 cut(s) 596
CspCI CAANNNNNGTGG 2 cut(s) 1087, 1122
CviAII CATG 4 cut(s) 266, 355, 504, 1144
CviQI GTAC 1 cut(s) 596
DdeI CTNAG 5 cut(s) 140, 252, 293, 873, 1155
DinI GGCGCC 1 cut(s) 578
DpnI GATC 6 cut(s) 451, 585, 621, 678, 861, 1148
DpnII GATC 6 cut(s) 449, 583, 619, 676, 859, 1146
DraIII CACNNNGTG 1 cut(s) 14
DriI GACNNNNNGTC 1 cut(s) 196
Eam1105I GACNNNNNGTC 1 cut(s) 196
Eco47I GGWCC 1 cut(s) 358
Eco57I CTGAAG 1 cut(s) 1152
Eco81I CCTNAGG 1 cut(s) 293
EgeI GGCGCC 1 cut(s) 578
EheI GGCGCC 1 cut(s) 578
FaeI CATG 4 cut(s) 269, 358, 507, 1147
FalI AAGNNNNNCTT 2 cut(s) 945, 977
FaqI GGGAC 3 cut(s) 344, 1040, 1108
FatI CATG 4 cut(s) 265, 354, 503, 1143
FauI CCCGC 1 cut(s) 152
FauNDI CATATG 1 cut(s) 202
FbaI TGATCA 2 cut(s) 583, 859
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 3 cut(s) 30, 33, 231
FokI GGATG 2 cut(s) 235, 863
Fsp4HI GCNGC 3 cut(s) 30, 33, 231
FspBI CTAG 2 cut(s) 257, 416
GlaI GCGC 1 cut(s) 578
GluI GCNGC 3 cut(s) 30, 33, 231
GsaI CCCAGC 1 cut(s) 1098
HaeII RGCGCY 1 cut(s) 580
HaeIII GGCC 2 cut(s) 146, 482
HapII CCGG 2 cut(s) 85, 245
HhaI GCGC 1 cut(s) 579
Hin1I GRCGYC 1 cut(s) 577
Hin1II CATG 4 cut(s) 269, 358, 507, 1147
Hin6I GCGC 1 cut(s) 577
HinP1I GCGC 1 cut(s) 577
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HinfI GANTC 2 cut(s) 278, 466
HpaI GTTAAC 1 cut(s) 667
HpaII CCGG 2 cut(s) 85, 245
HphI GGTGA 2 cut(s) 26, 971
Hpy166II GTNNAC 7 cut(s) 100, 112, 289, 406, 591, 667, 703
Hpy188I TCNGA 8 cut(s) 48, 216, 568, 724, 753, 874, 945, 1027
Hpy188III TCNNGA 6 cut(s) 499, 623, 786, 798, 1074, 1144
Hpy8I GTNNAC 7 cut(s) 100, 112, 289, 406, 591, 667, 703
HpyAV CCTTC 2 cut(s) 971, 1071
HpyCH4III ACNGT 3 cut(s) 595, 634, 1013
HpyCH4IV ACGT 1 cut(s) 925
HpyF10VI GCNNNNNNNGC 1 cut(s) 341
HpyF3I CTNAG 5 cut(s) 140, 252, 293, 873, 1155
HpySE526I ACGT 1 cut(s) 925
Hsp92I GRCGYC 1 cut(s) 577
Hsp92II CATG 4 cut(s) 269, 358, 507, 1147
HspAI GCGC 1 cut(s) 577
KasI GGCGCC 1 cut(s) 576
Ksp22I TGATCA 2 cut(s) 583, 859
KspAI GTTAAC 1 cut(s) 667
Kzo9I GATC 6 cut(s) 449, 583, 619, 676, 859, 1146
LmnI GCTCC 4 cut(s) 934, 1019, 1036, 1042
Lsp1109I GCAGC 2 cut(s) 41, 44
LweI GCATC 2 cut(s) 213, 572
MaeI CTAG 2 cut(s) 257, 416
MaeII ACGT 1 cut(s) 925
MaeIII GTNAC 1 cut(s) 169
MalI GATC 6 cut(s) 451, 585, 621, 678, 861, 1148
MboI GATC 6 cut(s) 449, 583, 619, 676, 859, 1146
MboII GAAGA 5 cut(s) 751, 754, 813, 1040, 1145
MfeI CAATTG 1 cut(s) 367
MflI RGATCY 2 cut(s) 449, 619
MhlI GDGCHC 2 cut(s) 166, 1095
Mly113I GGCGCC 1 cut(s) 577
MlyI GAGTC 2 cut(s) 272, 460
MmeI TCCRAC 1 cut(s) 255
MnlI CCTC 9 cut(s) 135, 201, 288, 453, 837, 868, 889, 900, 924
MroXI GAANNNNTTC 1 cut(s) 1137
MseI TTAA 4 cut(s) 666, 777, 809, 950
MslI CAYNNNNRTG 1 cut(s) 1088
MspI CCGG 2 cut(s) 85, 245
MunI CAATTG 1 cut(s) 367
MwoI GCNNNNNNNGC 1 cut(s) 341
NarI GGCGCC 1 cut(s) 577
NdeI CATATG 1 cut(s) 202
NdeII GATC 6 cut(s) 449, 583, 619, 676, 859, 1146
NlaIII CATG 4 cut(s) 269, 358, 507, 1147
NlaIV GGNNCC 5 cut(s) 249, 360, 578, 936, 1044
NspI RCATGY 2 cut(s) 269, 507
OliI CACNNNNGTG 1 cut(s) 1088
PagI TCATGA 1 cut(s) 1143
PcsI WCGNNNNNNNCGW 1 cut(s) 1072
PdmI GAANNNNTTC 1 cut(s) 1137
PkrI GCNGC 3 cut(s) 31, 34, 232
PleI GAGTC 2 cut(s) 272, 460
PluTI GGCGCC 1 cut(s) 580
PpsI GAGTC 2 cut(s) 272, 460
PspFI CCCAGC 1 cut(s) 1094
PspN4I GGNNCC 5 cut(s) 249, 360, 578, 936, 1044
PspPI GGNCC 2 cut(s) 144, 358
PstNI CAGNNNCTG 1 cut(s) 623
PsuI RGATCY 2 cut(s) 449, 619
RsaI GTAC 1 cut(s) 597
RsaNI GTAC 1 cut(s) 596
RseI CAYNNNNRTG 1 cut(s) 1088
SaqAI TTAA 4 cut(s) 666, 777, 809, 950
SatI GCNGC 3 cut(s) 30, 33, 231
Sau3AI GATC 6 cut(s) 449, 583, 619, 676, 859, 1146
Sau96I GGNCC 2 cut(s) 144, 358
ScaI AGTACT 1 cut(s) 597
SchI GAGTC 2 cut(s) 272, 460
SduI GDGCHC 2 cut(s) 166, 1095
SfaNI GCATC 2 cut(s) 213, 572
SfcI CTRYAG 1 cut(s) 193
SfoI GGCGCC 1 cut(s) 578
SinI GGWCC 1 cut(s) 358
SmiMI CAYNNNNRTG 1 cut(s) 1088
SsiI CCGC 2 cut(s) 159, 231
SspDI GGCGCC 1 cut(s) 576
SspI AATATT 1 cut(s) 43
SspMI CTAG 2 cut(s) 257, 416
TaaI ACNGT 3 cut(s) 595, 634, 1013
TaiI ACGT 1 cut(s) 928
TaqI TCGA 2 cut(s) 762, 1075
TatI WGTACW 1 cut(s) 595
TauI GCSGC 1 cut(s) 233
Tru1I TTAA 4 cut(s) 666, 777, 809, 950
Tru9I TTAA 4 cut(s) 666, 777, 809, 950
TscAI CASTG 2 cut(s) 15, 1018
TseI GCWGC 2 cut(s) 29, 32
TspDTI ATGAA 1 cut(s) 642
TspGWI ACGGA 1 cut(s) 141
TspRI CASTG 2 cut(s) 15, 1018
VpaK11BI GGWCC 1 cut(s) 358
XapI RAATTY 4 cut(s) 176, 184, 397, 779
XceI RCATGY 2 cut(s) 269, 507
XmiI GTMKAC 1 cut(s) 111
XmnI GAANNNNTTC 1 cut(s) 1137
XspI CTAG 2 cut(s) 257, 416
ZrmI AGTACT 1 cut(s) 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.