Rh2BG079600

Belongs to the tetraspanin (TM4SF) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0029462
Physical Location & Seq
Forward (+)
6079900 .. 6080409
510 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG079600.1

Sequence Viewer

Length: 510 bp
ATGGGAGTGAGCAACAAAATCACGGCAGTCCTCAACTTCACGGCGCTCCTCTGCTCCATCCCCATAATGGCCGCCGGCATCTGGCTGGCCAACAAGCCGGACAACGAGTGCATCCGCTCTTTCCGGTGGCCAATTTTCGTCCTCGGCGTCCTCATCCTCCTAGTCTCCCTCGCCGGCTTCATCGGGGCCTACTTCAACAAACAGGGCCTTCTTGCTCTCTACCTCTTCTGTATGGCCGCCCTCATAATCCTCCTACTTGTTGTGCTTATATTTGCTTTTACTGTCACGAAGCCCGACGGAAGCTACGGCGTTCCGGGTAGGGCTTACCGGGAGTACCGGCTGGACGGGTTTTCTAAGTGGCTAAGGGATTACGTTACTGATTCCGGGAACTGGCAGAAGATAAGGGTCTGCTTGGCTGAATCGGATACTTGTCCTAACCTCACTCAGAATTACATCACTGCTGATCAGTTCTTTGCGGCTCACATCTCTCCTCTTCAGGCAAGCTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

169

Amino Acids

18.81

Weight (kDa)

8.62

Isoelectric Point (pI)

32.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 5 - 139 1.2e-23 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0029462)

Species Orthologous Gene IDs
pyrus_communis pycom15g18210
rosa_samantha Rh2BG079600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 117
AciI CCGC 4 cut(s) 72, 115, 237, 476
AcoI YGGCCR 4 cut(s) 69, 87, 128, 234
AcuI CTGAAG 1 cut(s) 479
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 1 cut(s) 335
AfiI CCNNNNNNNGG 3 cut(s) 67, 81, 390
AgsI TTSAA 1 cut(s) 196
AluBI AGCT 2 cut(s) 303, 504
AluI AGCT 2 cut(s) 303, 504
Alw26I GTCTC 1 cut(s) 169
AoxI GGCC 6 cut(s) 69, 87, 128, 186, 205, 234
AspLEI GCGC 1 cut(s) 46
AspS9I GGNCC 2 cut(s) 186, 205
AsuC2I CCSGG 3 cut(s) 315, 329, 385
BalI TGGCCA 2 cut(s) 89, 130
BccI CCATC 1 cut(s) 65
BceAI ACGGC 3 cut(s) 39, 57, 322
BciVI GTATCC 1 cut(s) 418
BclI TGATCA 1 cut(s) 463
BcnI CCSGG 3 cut(s) 315, 329, 385
BcoDI GTCTC 1 cut(s) 169
BfaI CTAG 1 cut(s) 161
BfoI RGCGCY 1 cut(s) 47
BfuI GTATCC 1 cut(s) 418
BisI GCNGC 3 cut(s) 72, 237, 477
BlsI GCNGC 3 cut(s) 73, 238, 478
Bme1390I CCNGG 3 cut(s) 315, 329, 385
BmgT120I GGNCC 2 cut(s) 186, 205
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 3 cut(s) 315, 329, 385
BmsI GCATC 2 cut(s) 87, 120
Bpu10I CCTNAGC 1 cut(s) 362
BpuMI CCSGG 3 cut(s) 315, 329, 385
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 1 cut(s) 142
BsaWI WCCGGW 1 cut(s) 123
Bsc4I CCNNNNNNNGG 3 cut(s) 67, 81, 390
Bse118I RCCGGY 3 cut(s) 74, 173, 336
Bse1I ACTGG 1 cut(s) 395
BseDI CCNNGG 1 cut(s) 142
BseGI GGATG 3 cut(s) 57, 111, 153
BseLI CCNNNNNNNGG 3 cut(s) 67, 81, 390
BseMII CTCAG 1 cut(s) 458
BseNI ACTGG 1 cut(s) 395
BseRI GAGGAG 2 cut(s) 38, 480
BshFI GGCC 6 cut(s) 71, 89, 130, 188, 207, 236
BsiSI CCGG 8 cut(s) 75, 98, 124, 174, 314, 328, 337, 384
BslI CCNNNNNNNGG 3 cut(s) 67, 81, 390
BsmAI GTCTC 1 cut(s) 169
BsnI GGCC 6 cut(s) 71, 89, 130, 188, 207, 236
Bsp143I GATC 1 cut(s) 463
BspACI CCGC 4 cut(s) 72, 115, 237, 476
BspANI GGCC 6 cut(s) 71, 89, 130, 188, 207, 236
BspCNI CTCAG 1 cut(s) 457
BspLI GGNNCC 1 cut(s) 187
BsrBI CCGCTC 1 cut(s) 117
BsrFI RCCGGY 3 cut(s) 74, 173, 336
BsrI ACTGG 1 cut(s) 395
BssAI RCCGGY 3 cut(s) 74, 173, 336
BssECI CCNNGG 1 cut(s) 142
BssMI GATC 1 cut(s) 463
BssNI GRCGYC 1 cut(s) 147
Bst4CI ACNGT 1 cut(s) 283
Bst6I CTCTTC 2 cut(s) 230, 498
BstACI GRCGYC 1 cut(s) 147
BstC8I GCNNGC 4 cut(s) 76, 87, 175, 502
BstDEI CTNAG 3 cut(s) 354, 362, 444
BstF5I GGATG 3 cut(s) 57, 111, 153
BstH2I RGCGCY 1 cut(s) 47
BstHHI GCGC 1 cut(s) 46
BstKTI GATC 1 cut(s) 466
BstMAI GTCTC 1 cut(s) 169
BstMBI GATC 1 cut(s) 463
BstSCI CCNGG 3 cut(s) 313, 327, 383
BsuI GTATCC 1 cut(s) 418
BsuRI GGCC 6 cut(s) 71, 89, 130, 188, 207, 236
BtsCI GGATG 3 cut(s) 57, 111, 153
BtsI GCAGTG 1 cut(s) 456
BtsIMutI CAGTG 1 cut(s) 456
Cac8I GCNNGC 4 cut(s) 76, 87, 175, 502
CfoI GCGC 1 cut(s) 46
Cfr10I RCCGGY 3 cut(s) 74, 173, 336
Cfr13I GGNCC 2 cut(s) 186, 205
CseI GACGC 1 cut(s) 136
Csp6I GTAC 1 cut(s) 334
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 3 cut(s) 354, 362, 444
DpnI GATC 1 cut(s) 465
DpnII GATC 1 cut(s) 463
EaeI YGGCCR 4 cut(s) 69, 87, 128, 234
Eam1104I CTCTTC 2 cut(s) 230, 498
EarI CTCTTC 2 cut(s) 230, 498
Eco57I CTGAAG 1 cut(s) 479
EcoO109I RGGNCCY 2 cut(s) 186, 205
FaiI YATR 4 cut(s) 65, 233, 245, 269
FbaI TGATCA 1 cut(s) 463
Fnu4HI GCNGC 3 cut(s) 72, 237, 477
FokI GGATG 3 cut(s) 44, 98, 140
Fsp4HI GCNGC 3 cut(s) 72, 237, 477
FspBI CTAG 1 cut(s) 161
GlaI GCGC 1 cut(s) 45
GluI GCNGC 3 cut(s) 72, 237, 477
HaeII RGCGCY 1 cut(s) 47
HaeIII GGCC 6 cut(s) 71, 89, 130, 188, 207, 236
HapII CCGG 8 cut(s) 75, 98, 124, 174, 314, 328, 337, 384
HgaI GACGC 1 cut(s) 136
HhaI GCGC 1 cut(s) 46
Hin1I GRCGYC 1 cut(s) 147
Hin6I GCGC 1 cut(s) 44
HinP1I GCGC 1 cut(s) 44
HinfI GANTC 2 cut(s) 380, 419
HpaII CCGG 8 cut(s) 75, 98, 124, 174, 314, 328, 337, 384
Hpy188I TCNGA 2 cut(s) 424, 447
Hpy188III TCNNGA 1 cut(s) 286
Hpy99I CGWCG 1 cut(s) 299
HpyAV CCTTC 1 cut(s) 218
HpyCH4III ACNGT 1 cut(s) 283
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 1 cut(s) 111
HpyF3I CTNAG 3 cut(s) 354, 362, 444
HpySE526I ACGT 1 cut(s) 372
Hsp92I GRCGYC 1 cut(s) 147
HspAI GCGC 1 cut(s) 44
KroI GCCGGC 2 cut(s) 74, 173
KroNI GCCGGC 2 cut(s) 76, 175
Ksp22I TGATCA 1 cut(s) 463
Kzo9I GATC 1 cut(s) 463
LmnI GCTCC 2 cut(s) 51, 59
LweI GCATC 2 cut(s) 87, 120
MaeI CTAG 1 cut(s) 161
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 2 cut(s) 283, 373
MalI GATC 1 cut(s) 465
MbiI CCGCTC 1 cut(s) 117
MboI GATC 1 cut(s) 463
MboII GAAGA 3 cut(s) 217, 409, 485
MlsI TGGCCA 2 cut(s) 89, 130
MluCI AATT 2 cut(s) 132, 448
MluNI TGGCCA 2 cut(s) 89, 130
Mox20I TGGCCA 2 cut(s) 89, 130
MroNI GCCGGC 2 cut(s) 74, 173
MscI TGGCCA 2 cut(s) 89, 130
MseI TTAA 1 cut(s) 508
Msp20I TGGCCA 2 cut(s) 89, 130
MspI CCGG 8 cut(s) 75, 98, 124, 174, 314, 328, 337, 384
MspR9I CCNGG 3 cut(s) 315, 329, 385
NaeI GCCGGC 2 cut(s) 76, 175
NciI CCSGG 3 cut(s) 315, 329, 385
NdeII GATC 1 cut(s) 463
NgoMIV GCCGGC 2 cut(s) 74, 173
NlaIV GGNNCC 1 cut(s) 187
NmeAIII GCCGAG 1 cut(s) 123
NmuCI GTSAC 1 cut(s) 283
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PdiI GCCGGC 2 cut(s) 76, 175
PfeI GAWTC 2 cut(s) 380, 419
PfoI TCCNGGA 1 cut(s) 383
PkrI GCNGC 3 cut(s) 73, 238, 478
PspN4I GGNNCC 1 cut(s) 187
PspPI GGNCC 2 cut(s) 186, 205
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
SaqAI TTAA 1 cut(s) 508
SatI GCNGC 3 cut(s) 72, 237, 477
Sau3AI GATC 1 cut(s) 463
Sau96I GGNCC 2 cut(s) 186, 205
ScrFI CCNGG 3 cut(s) 315, 329, 385
SetI ASST 5 cut(s) 225, 305, 375, 441, 506
SfaNI GCATC 2 cut(s) 87, 120
Sse9I AATT 2 cut(s) 132, 448
SsiI CCGC 4 cut(s) 72, 115, 237, 476
SspMI CTAG 1 cut(s) 161
StyD4I CCNGG 3 cut(s) 313, 327, 383
TaaI ACNGT 1 cut(s) 283
TaiI ACGT 1 cut(s) 375
TasI AATT 2 cut(s) 132, 448
TauI GCSGC 3 cut(s) 74, 239, 479
TfiI GAWTC 2 cut(s) 380, 419
Tru1I TTAA 1 cut(s) 508
Tru9I TTAA 1 cut(s) 508
TscAI CASTG 1 cut(s) 463
TseFI GTSAC 1 cut(s) 283
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 1 cut(s) 169
TspGWI ACGGA 1 cut(s) 312
TspRI CASTG 1 cut(s) 463
XcmI CCANNNNNNNNNTGG 1 cut(s) 64
XspI CTAG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.