Rh2BG239200

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0024870
Physical Location & Seq
Reverse (-)
23821240 .. 23821907
668 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG239200.1

Sequence Viewer

Length: 348 bp
ATGGGTTCCTCCGCCGTTCCTATTTCTGACACCGTGAAGAAAATATGGGATAAGTGGAACCTCCGGGGCTTCATCCTATTAAGCCTATCATTGCAGACCTTTTTAATTCTTTGTGCACCCATGAGAAAGAGAACATCAAGCAAATGGGTGATCGTGCCCATTTGGTTAGCTTACTTGCTCGCAGACTGGGCTGCTAACTTTGCCATTGGATTAATTGCAAGCAGCCAAACTGACACCACCGGTCGCCGAGACAATGGCGGCCTTTTGGCATTTTGGGCTCCATTTCTTTTGTTACACCTGGGTGGTCCGGAGTCCGGACACCATAATTGCGATTTCTATGGAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

115

Amino Acids

12.8

Weight (kDa)

9.44

Isoelectric Point (pI)

39.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 55 - 105 1.2e-14 Domain of unknown function (DUF4220)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0024870)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0111481
rosa_samantha Rh2BG239200
rosa_wichuraiana Rw2G018380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 307, 314
AciI CCGC 2 cut(s) 12, 258
AfiI CCNNNNNNNGG 1 cut(s) 314
AgeI ACCGGT 1 cut(s) 239
AjnI CCWGG 1 cut(s) 297
AleI CACNNNNGTG 1 cut(s) 300
AluBI AGCT 1 cut(s) 170
AluI AGCT 1 cut(s) 170
Alw21I GWGCWC 1 cut(s) 118
Alw26I GTCTC 1 cut(s) 243
Alw44I GTGCAC 1 cut(s) 114
Aor13HI TCCGGA 2 cut(s) 307, 314
AoxI GGCC 1 cut(s) 259
ApaLI GTGCAC 1 cut(s) 114
ApeKI GCWGC 2 cut(s) 191, 222
AseI ATTAAT 1 cut(s) 212
AsiGI ACCGGT 1 cut(s) 239
AspS9I GGNCC 1 cut(s) 305
AsuC2I CCSGG 1 cut(s) 65
AsuHPI GGTGA 1 cut(s) 160
AvaII GGWCC 1 cut(s) 305
BaeGI GKGCMC 2 cut(s) 118, 159
BanII GRGCYC 1 cut(s) 280
Bbv12I GWGCWC 1 cut(s) 118
BbvI GCAGC 2 cut(s) 178, 234
BciT130I CCWGG 1 cut(s) 299
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 1 cut(s) 243
BisI GCNGC 3 cut(s) 192, 223, 259
BlsI GCNGC 3 cut(s) 193, 224, 260
Bme1390I CCNGG 2 cut(s) 65, 299
Bme18I GGWCC 1 cut(s) 305
BmgT120I GGNCC 1 cut(s) 305
BmiI GGNNCC 3 cut(s) 7, 59, 279
BmrFI CCNGG 2 cut(s) 65, 299
BmrI ACTGGG 1 cut(s) 196
BmuI ACTGGG 1 cut(s) 196
BpuMI CCSGG 1 cut(s) 65
BsaJI CCNNGG 2 cut(s) 64, 298
BsaWI WCCGGW 3 cut(s) 239, 307, 314
Bsc4I CCNNNNNNNGG 1 cut(s) 314
Bse118I RCCGGY 1 cut(s) 239
Bse1I ACTGG 1 cut(s) 191
Bse3DI GCAATG 1 cut(s) 89
BseAI TCCGGA 2 cut(s) 307, 314
BseBI CCWGG 1 cut(s) 299
BseDI CCNNGG 2 cut(s) 64, 298
BseGI GGATG 1 cut(s) 72
BseLI CCNNNNNNNGG 1 cut(s) 314
BseMI GCAATG 1 cut(s) 89
BseNI ACTGG 1 cut(s) 191
BseSI GKGCMC 2 cut(s) 118, 159
BseXI GCAGC 2 cut(s) 178, 234
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 1 cut(s) 261
BshTI ACCGGT 1 cut(s) 239
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 1 cut(s) 118
BsiSI CCGG 4 cut(s) 64, 240, 308, 315
BslI CCNNNNNNNGG 1 cut(s) 314
BsmAI GTCTC 1 cut(s) 243
BsnI GGCC 1 cut(s) 261
Bsp1286I GDGCHC 3 cut(s) 118, 159, 280
Bsp13I TCCGGA 2 cut(s) 307, 314
Bsp143I GATC 1 cut(s) 150
BspACI CCGC 2 cut(s) 12, 258
BspANI GGCC 1 cut(s) 261
BspEI TCCGGA 2 cut(s) 307, 314
BspLI GGNNCC 3 cut(s) 7, 59, 279
BsrDI GCAATG 1 cut(s) 89
BsrFI RCCGGY 1 cut(s) 239
BsrI ACTGG 1 cut(s) 191
BssAI RCCGGY 1 cut(s) 239
BssECI CCNNGG 2 cut(s) 64, 298
BssMI GATC 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 299
Bst4CI ACNGT 1 cut(s) 34
BstC8I GCNNGC 2 cut(s) 180, 220
BstF5I GGATG 1 cut(s) 72
BstKTI GATC 1 cut(s) 153
BstMAI GTCTC 1 cut(s) 243
BstMBI GATC 1 cut(s) 150
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 3 cut(s) 188, 200, 275
BstNI CCWGG 1 cut(s) 299
BstSCI CCNGG 2 cut(s) 63, 297
BstSLI GKGCMC 2 cut(s) 118, 159
BstV1I GCAGC 2 cut(s) 178, 234
BsuRI GGCC 1 cut(s) 261
BtsCI GGATG 1 cut(s) 72
Cac8I GCNNGC 2 cut(s) 180, 220
Cfr10I RCCGGY 1 cut(s) 239
Cfr13I GGNCC 1 cut(s) 305
CspAI ACCGGT 1 cut(s) 239
CviAII CATG 1 cut(s) 121
CviJI RGCY 7 cut(s) 69, 84, 170, 191, 225, 261, 278
CviKI_1 RGCY 7 cut(s) 69, 84, 170, 191, 225, 261, 278
DpnI GATC 1 cut(s) 152
DpnII GATC 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 280
Eco47I GGWCC 1 cut(s) 305
EcoRII CCWGG 1 cut(s) 297
EcoT38I GRGCYC 1 cut(s) 280
FaeI CATG 1 cut(s) 124
FaiI YATR 4 cut(s) 46, 122, 324, 339
FatI CATG 1 cut(s) 120
Fnu4HI GCNGC 3 cut(s) 192, 223, 259
FokI GGATG 1 cut(s) 59
FriOI GRGCYC 1 cut(s) 280
Fsp4HI GCNGC 3 cut(s) 192, 223, 259
GluI GCNGC 3 cut(s) 192, 223, 259
HaeIII GGCC 1 cut(s) 261
HapII CCGG 4 cut(s) 64, 240, 308, 315
Hin1II CATG 1 cut(s) 124
HinfI GANTC 1 cut(s) 311
HpaII CCGG 4 cut(s) 64, 240, 308, 315
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 1 cut(s) 116
Hpy188I TCNGA 1 cut(s) 28
Hpy188III TCNNGA 2 cut(s) 308, 315
Hpy8I GTNNAC 1 cut(s) 116
HpyCH4III ACNGT 1 cut(s) 34
HpyCH4V TGCA 3 cut(s) 94, 116, 218
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 200, 275
Hsp92II CATG 1 cut(s) 124
Kpn2I TCCGGA 2 cut(s) 307, 314
Kzo9I GATC 1 cut(s) 150
LmnI GCTCC 1 cut(s) 283
LpnPI CCDG 7 cut(s) 77, 172, 253, 284, 311, 321, 328
Lsp1109I GCAGC 2 cut(s) 178, 234
MaeIII GTNAC 1 cut(s) 291
MalI GATC 1 cut(s) 152
MboI GATC 1 cut(s) 150
MboII GAAGA 1 cut(s) 49
MhlI GDGCHC 3 cut(s) 118, 159, 280
MluCI AATT 3 cut(s) 105, 213, 325
MlyI GAGTC 1 cut(s) 320
MnlI CCTC 2 cut(s) 19, 71
MroI TCCGGA 2 cut(s) 307, 314
MseI TTAA 3 cut(s) 80, 104, 212
MslI CAYNNNNRTG 1 cut(s) 300
MspI CCGG 4 cut(s) 64, 240, 308, 315
MspR9I CCNGG 2 cut(s) 65, 299
MvaI CCWGG 1 cut(s) 299
MwoI GCNNNNNNNGC 3 cut(s) 188, 200, 275
NciI CCSGG 1 cut(s) 65
NdeII GATC 1 cut(s) 150
NlaIII CATG 1 cut(s) 124
NlaIV GGNNCC 3 cut(s) 7, 59, 279
NmeAIII GCCGAG 1 cut(s) 272
OliI CACNNNNGTG 1 cut(s) 300
PinAI ACCGGT 1 cut(s) 239
PkrI GCNGC 3 cut(s) 193, 224, 260
PleI GAGTC 1 cut(s) 319
PpsI GAGTC 1 cut(s) 319
PshBI ATTAAT 1 cut(s) 212
Psp6I CCWGG 1 cut(s) 297
PspGI CCWGG 1 cut(s) 297
PspN4I GGNNCC 3 cut(s) 7, 59, 279
PspPI GGNCC 1 cut(s) 305
RseI CAYNNNNRTG 1 cut(s) 300
SaqAI TTAA 3 cut(s) 80, 104, 212
SatI GCNGC 3 cut(s) 192, 223, 259
Sau3AI GATC 1 cut(s) 150
Sau96I GGNCC 1 cut(s) 305
SchI GAGTC 1 cut(s) 320
ScrFI CCNGG 2 cut(s) 65, 299
SduI GDGCHC 3 cut(s) 118, 159, 280
SetI ASST 4 cut(s) 63, 101, 172, 300
SinI GGWCC 1 cut(s) 305
SmiMI CAYNNNNRTG 1 cut(s) 300
Sse9I AATT 3 cut(s) 105, 213, 325
SsiI CCGC 2 cut(s) 12, 258
StyD4I CCNGG 2 cut(s) 63, 297
TaaI ACNGT 1 cut(s) 34
TasI AATT 3 cut(s) 105, 213, 325
TauI GCSGC 1 cut(s) 261
Tru1I TTAA 3 cut(s) 80, 104, 212
Tru9I TTAA 3 cut(s) 80, 104, 212
TseI GCWGC 2 cut(s) 191, 222
TspDTI ATGAA 1 cut(s) 61
VneI GTGCAC 1 cut(s) 114
VpaK11BI GGWCC 1 cut(s) 305
VspI ATTAAT 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.