Rw2G018380

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0024870
Physical Location & Seq
Reverse (-)
23578144 .. 23578464
321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G018380.1

Sequence Viewer

Length: 321 bp
ATGGCTTCATTAGCCGCTCCTATTTCTAACAAGGTCAAGAAGATCTGGGATAAGTGGAATCTGCGGAGCTTCATCATATTAAGTCTCTCATTGCAAACCTTCTTAATTCTCTTTGCACCCTTGAGAAAGAGAACATCGAGCAAATGGGTAATCGTGCCTATCTGGTTAGCTTACTTGCTCGCTGACTGGGCTGCTAACTTTGCTATCGGACTAATTGCAAGCAGCCAAACGAACACTGATCAAGGTTCCCTAGAGAATGGTAATCTCTTGACATTCTGGGCTCCCTTTCTTCTTTTGCACCTTGGTGGTCCAGACCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

106

Amino Acids

11.87

Weight (kDa)

9.87

Isoelectric Point (pI)

36.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 55 - 105 4.5e-15 Domain of unknown function (DUF4220)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0024870)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0111481
rosa_samantha Rh2BG239200
rosa_wichuraiana Rw2G018380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 17
AciI CCGC 2 cut(s) 15, 64
AleI CACNNNNGTG 1 cut(s) 303
AluBI AGCT 2 cut(s) 69, 170
AluI AGCT 2 cut(s) 69, 170
Alw26I GTCTC 1 cut(s) 89
ApeKI GCWGC 2 cut(s) 191, 222
AspS9I GGNCC 1 cut(s) 308
AvaII GGWCC 1 cut(s) 308
BanII GRGCYC 1 cut(s) 283
BbvI GCAGC 2 cut(s) 178, 234
BclI TGATCA 1 cut(s) 238
BcoDI GTCTC 1 cut(s) 89
BfaI CTAG 1 cut(s) 251
BglII AGATCT 1 cut(s) 42
BisI GCNGC 3 cut(s) 15, 192, 223
BlsI GCNGC 3 cut(s) 16, 193, 224
Bme18I GGWCC 1 cut(s) 308
BmgT120I GGNCC 1 cut(s) 308
BmiI GGNNCC 2 cut(s) 247, 282
BmrI ACTGGG 1 cut(s) 196
BmuI ACTGGG 1 cut(s) 196
BpuEI CTTGAG 1 cut(s) 142
BsaJI CCNNGG 1 cut(s) 301
Bse1I ACTGG 1 cut(s) 191
Bse3DI GCAATG 1 cut(s) 89
BseDI CCNNGG 1 cut(s) 301
BseMI GCAATG 1 cut(s) 89
BseNI ACTGG 1 cut(s) 191
BseXI GCAGC 2 cut(s) 178, 234
BsmAI GTCTC 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 283
Bsp143I GATC 2 cut(s) 42, 238
BspACI CCGC 2 cut(s) 15, 64
BspLI GGNNCC 2 cut(s) 247, 282
BsrBI CCGCTC 1 cut(s) 17
BsrDI GCAATG 1 cut(s) 89
BsrI ACTGG 1 cut(s) 191
BssECI CCNNGG 1 cut(s) 301
BssMI GATC 2 cut(s) 42, 238
BssT1I CCWWGG 1 cut(s) 301
BstC8I GCNNGC 2 cut(s) 180, 220
BstKTI GATC 2 cut(s) 45, 241
BstMAI GTCTC 1 cut(s) 89
BstMBI GATC 2 cut(s) 42, 238
BstMWI GCNNNNNNNGC 3 cut(s) 11, 188, 200
BstV1I GCAGC 2 cut(s) 178, 234
BstX2I RGATCY 1 cut(s) 42
BstYI RGATCY 1 cut(s) 42
BtsIMutI CAGTG 1 cut(s) 234
Cac8I GCNNGC 2 cut(s) 180, 220
Cfr13I GGNCC 1 cut(s) 308
CviJI RGCY 7 cut(s) 5, 14, 69, 170, 191, 225, 281
CviKI_1 RGCY 7 cut(s) 5, 14, 69, 170, 191, 225, 281
DpnI GATC 2 cut(s) 44, 240
DpnII GATC 2 cut(s) 42, 238
Eco130I CCWWGG 1 cut(s) 301
Eco24I GRGCYC 1 cut(s) 283
Eco47I GGWCC 1 cut(s) 308
EcoT14I CCWWGG 1 cut(s) 301
EcoT38I GRGCYC 1 cut(s) 283
ErhI CCWWGG 1 cut(s) 301
FaiI YATR 1 cut(s) 77
FbaI TGATCA 1 cut(s) 238
Fnu4HI GCNGC 3 cut(s) 15, 192, 223
FriOI GRGCYC 1 cut(s) 283
Fsp4HI GCNGC 3 cut(s) 15, 192, 223
FspBI CTAG 1 cut(s) 251
GluI GCNGC 3 cut(s) 15, 192, 223
HinfI GANTC 1 cut(s) 58
Hpy188I TCNGA 1 cut(s) 209
Hpy188III TCNNGA 3 cut(s) 37, 268, 311
HpyAV CCTTC 1 cut(s) 109
HpyCH4V TGCA 4 cut(s) 94, 116, 218, 298
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 188, 200
Ksp22I TGATCA 1 cut(s) 238
Kzo9I GATC 2 cut(s) 42, 238
LmnI GCTCC 3 cut(s) 22, 66, 286
LpnPI CCDG 4 cut(s) 31, 148, 172, 262
Lsp1109I GCAGC 2 cut(s) 178, 234
MaeI CTAG 1 cut(s) 251
MalI GATC 2 cut(s) 44, 240
MbiI CCGCTC 1 cut(s) 17
MboI GATC 2 cut(s) 42, 238
MboII GAAGA 2 cut(s) 52, 281
MflI RGATCY 1 cut(s) 42
MhlI GDGCHC 1 cut(s) 283
MluCI AATT 2 cut(s) 105, 213
MseI TTAA 2 cut(s) 80, 104
MslI CAYNNNNRTG 1 cut(s) 303
MwoI GCNNNNNNNGC 3 cut(s) 11, 188, 200
NdeII GATC 2 cut(s) 42, 238
NlaIV GGNNCC 2 cut(s) 247, 282
OliI CACNNNNGTG 1 cut(s) 303
PfeI GAWTC 1 cut(s) 58
PkrI GCNGC 3 cut(s) 16, 193, 224
PspN4I GGNNCC 2 cut(s) 247, 282
PspPI GGNCC 1 cut(s) 308
PsuI RGATCY 1 cut(s) 42
RseI CAYNNNNRTG 1 cut(s) 303
SaqAI TTAA 2 cut(s) 80, 104
SatI GCNGC 3 cut(s) 15, 192, 223
Sau3AI GATC 2 cut(s) 42, 238
Sau96I GGNCC 1 cut(s) 308
SduI GDGCHC 1 cut(s) 283
SetI ASST 6 cut(s) 36, 71, 101, 172, 247, 303
SinI GGWCC 1 cut(s) 308
SmiMI CAYNNNNRTG 1 cut(s) 303
SmlI CTYRAG 1 cut(s) 121
SmoI CTYRAG 1 cut(s) 121
Sse9I AATT 2 cut(s) 105, 213
SsiI CCGC 2 cut(s) 15, 64
SspMI CTAG 1 cut(s) 251
StyI CCWWGG 1 cut(s) 301
TaqI TCGA 1 cut(s) 137
TasI AATT 2 cut(s) 105, 213
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 1 cut(s) 58
Tru1I TTAA 2 cut(s) 80, 104
Tru9I TTAA 2 cut(s) 80, 104
TscAI CASTG 1 cut(s) 241
TseI GCWGC 2 cut(s) 191, 222
TspDTI ATGAA 1 cut(s) 61
TspRI CASTG 1 cut(s) 241
VpaK11BI GGWCC 1 cut(s) 308
XspI CTAG 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.