Rh2BG256700

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0033033
Physical Location & Seq
Reverse (-)
26882092 .. 26907135
25044 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG256700.1

Sequence Viewer

Length: 664 bp
ATGGTGAATACAGCAAATTATGGGGTTTTGTTTATGCTACTAAGTATGAGGAAGTACTTGCTCAAAGCCGTATGGAGGCACCTGGGATTTGCCGATGATGAATACTTCATTTGGAACTCATTTTACTGTGGTGATATCTTGGGACTAGACTTGTCATCCAATTCTCTAATTGGACCTCTATCAGATGGTGTTGGTAATTTGAAAGTTGTTGTATACATAAATGTATCATACAACCAATTATCTGGTAGTGTACCAAGTAGCATTGGAAGTTTATTGAGTTTGGTCAATCTCTCCTTAGCACATAACAATCTAGAAGGCCCTATTCCTAGTTCCTTTGCAGATTGTGTAAGCTTAGAAGGATTGGATTTATCTCGAAATAGTTTCACTGGAGTGATTCCAAAGTCTCTAGAAGCACTCTTATATCTCAAGCATCTGAATTTGTCTTTCAACAGACTCCAAGGAGAAATTCCAAGCGGCGGTCCTTTCAAAAACTTCTCTGCTGAATCATTTGTTTCAAACCATGGACTCTGTGGTGAATCCCAGTTTCAAGTTCCACCATGCAAAAGAAAAACTAGCATATCTATCTTGAAGTCCATTGAGGAGGTGGACCTCATGAGGTTCATGATTGAGGAAGAGGTGCTGAAGAGCTCTAACAGATTAGCTG

Protein Analysis

221

Amino Acids

24.31

Weight (kDa)

5.8

Isoelectric Point (pI)

30.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 93 - 152 4.4e-09 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0033033)

Species Orthologous Gene IDs
rosa_samantha Rh2BG256700 Rh2BG257100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 78
AccI GTMKAC 1 cut(s) 213
AciI CCGC 2 cut(s) 474, 477
AcsI RAATTY 2 cut(s) 436, 465
AcuI CTGAAG 1 cut(s) 662
AfaI GTAC 2 cut(s) 56, 252
AfiI CCNNNNNNNGG 2 cut(s) 75, 476
AgsI TTSAA 6 cut(s) 202, 448, 487, 516, 548, 589
AjnI CCWGG 1 cut(s) 81
AjuI GAANNNNNNNTTGG 2 cut(s) 450, 482
AleI CACNNNNGTG 1 cut(s) 389
AluBI AGCT 3 cut(s) 351, 648, 662
AluI AGCT 3 cut(s) 351, 648, 662
Alw21I GWGCWC 1 cut(s) 650
Alw26I GTCTC 1 cut(s) 408
AoxI GGCC 1 cut(s) 316
ApoI RAATTY 2 cut(s) 436, 465
AspS9I GGNCC 4 cut(s) 173, 317, 479, 607
AsuHPI GGTGA 3 cut(s) 16, 143, 545
AvaII GGWCC 3 cut(s) 173, 479, 607
BanI GGYRCC 1 cut(s) 78
BanII GRGCYC 1 cut(s) 650
Bbv12I GWGCWC 1 cut(s) 650
BccI CCATC 1 cut(s) 179
BceAI ACGGC 1 cut(s) 53
BciT130I CCWGG 1 cut(s) 83
BcoDI GTCTC 1 cut(s) 408
BfaI CTAG 5 cut(s) 146, 311, 327, 407, 573
BisI GCNGC 1 cut(s) 475
BlsI GCNGC 1 cut(s) 476
BmcAI AGTACT 1 cut(s) 56
Bme1390I CCNGG 1 cut(s) 83
Bme18I GGWCC 3 cut(s) 173, 479, 607
BmgT120I GGNCC 4 cut(s) 173, 317, 479, 607
BmiI GGNNCC 1 cut(s) 80
BmrFI CCNGG 1 cut(s) 83
BmrI ACTGGG 1 cut(s) 535
BmsI GCATC 1 cut(s) 439
BmuI ACTGGG 1 cut(s) 535
BpmI CTGGAG 1 cut(s) 408
Bpu10I CCTNAGC 1 cut(s) 295
BpuEI CTTGAG 1 cut(s) 410
BsaJI CCNNGG 3 cut(s) 82, 457, 520
Bsc4I CCNNNNNNNGG 2 cut(s) 75, 476
Bse1I ACTGG 2 cut(s) 391, 541
BseBI CCWGG 1 cut(s) 83
BseDI CCNNGG 3 cut(s) 82, 457, 520
BseGI GGATG 1 cut(s) 155
BseLI CCNNNNNNNGG 2 cut(s) 75, 476
BseNI ACTGG 2 cut(s) 391, 541
BseRI GAGGAG 1 cut(s) 614
BshFI GGCC 1 cut(s) 318
BshNI GGYRCC 1 cut(s) 78
BsiHKAI GWGCWC 1 cut(s) 650
BslFI GGGAC 1 cut(s) 156
BslI CCNNNNNNNGG 2 cut(s) 75, 476
BsmAI GTCTC 1 cut(s) 408
BsmFI GGGAC 1 cut(s) 156
BsnI GGCC 1 cut(s) 318
Bsp1286I GDGCHC 1 cut(s) 650
Bsp19I CCATGG 1 cut(s) 520
BspACI CCGC 2 cut(s) 474, 477
BspANI GGCC 1 cut(s) 318
BspHI TCATGA 2 cut(s) 612, 621
BspLI GGNNCC 1 cut(s) 80
BspQI GCTCTTC 1 cut(s) 638
BspT107I GGYRCC 1 cut(s) 78
BsrI ACTGG 2 cut(s) 391, 541
BssECI CCNNGG 3 cut(s) 82, 457, 520
BssNAI GTATAC 1 cut(s) 214
BssT1I CCWWGG 2 cut(s) 457, 520
Bst1107I GTATAC 1 cut(s) 214
Bst2UI CCWGG 1 cut(s) 83
Bst4CI ACNGT 1 cut(s) 128
Bst6I CTCTTC 2 cut(s) 627, 638
BstDEI CTNAG 3 cut(s) 41, 295, 352
BstDSI CCRYGG 1 cut(s) 520
BstF5I GGATG 1 cut(s) 155
BstMAI GTCTC 1 cut(s) 408
BstNI CCWGG 1 cut(s) 83
BstSCI CCNGG 1 cut(s) 81
BstXI CCANNNNNNTGG 1 cut(s) 242
BstZ17I GTATAC 1 cut(s) 214
BsuRI GGCC 1 cut(s) 318
BtgI CCRYGG 1 cut(s) 520
BtsCI GGATG 1 cut(s) 155
BtsIMutI CAGTG 1 cut(s) 384
CciI TCATGA 2 cut(s) 612, 621
Cfr13I GGNCC 4 cut(s) 173, 317, 479, 607
Csp6I GTAC 2 cut(s) 55, 251
CviAII CATG 4 cut(s) 521, 558, 613, 622
CviJI RGCY 5 cut(s) 68, 318, 351, 648, 662
CviKI_1 RGCY 5 cut(s) 68, 318, 351, 648, 662
CviQI GTAC 2 cut(s) 55, 251
DdeI CTNAG 3 cut(s) 41, 295, 352
Eam1104I CTCTTC 2 cut(s) 627, 638
EarI CTCTTC 2 cut(s) 627, 638
Ecl136II GAGCTC 1 cut(s) 648
Eco130I CCWWGG 2 cut(s) 457, 520
Eco24I GRGCYC 1 cut(s) 650
Eco32I GATATC 1 cut(s) 136
Eco47I GGWCC 3 cut(s) 173, 479, 607
Eco53kI GAGCTC 1 cut(s) 648
Eco57I CTGAAG 1 cut(s) 662
EcoICRI GAGCTC 1 cut(s) 648
EcoO109I RGGNCCY 1 cut(s) 317
EcoRII CCWGG 1 cut(s) 81
EcoRV GATATC 1 cut(s) 136
EcoT14I CCWWGG 2 cut(s) 457, 520
EcoT38I GRGCYC 1 cut(s) 650
ErhI CCWWGG 2 cut(s) 457, 520
FaeI CATG 4 cut(s) 524, 561, 616, 625
FaqI GGGAC 1 cut(s) 156
FatI CATG 4 cut(s) 520, 557, 612, 621
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 1 cut(s) 475
FokI GGATG 1 cut(s) 142
FriOI GRGCYC 1 cut(s) 650
Fsp4HI GCNGC 1 cut(s) 475
FspBI CTAG 5 cut(s) 146, 311, 327, 407, 573
GluI GCNGC 1 cut(s) 475
GsuI CTGGAG 1 cut(s) 408
HaeIII GGCC 1 cut(s) 318
Hin1II CATG 4 cut(s) 524, 561, 616, 625
HindIII AAGCTT 1 cut(s) 349
HinfI GANTC 5 cut(s) 394, 453, 503, 525, 536
HphI GGTGA 3 cut(s) 16, 143, 545
Hpy166II GTNNAC 3 cut(s) 214, 251, 607
Hpy188I TCNGA 2 cut(s) 184, 435
Hpy188III TCNNGA 6 cut(s) 311, 372, 407, 586, 613, 622
Hpy8I GTNNAC 3 cut(s) 214, 251, 607
HpyAV CCTTC 2 cut(s) 308, 350
HpyCH4III ACNGT 1 cut(s) 128
HpyCH4V TGCA 2 cut(s) 338, 561
HpyF3I CTNAG 3 cut(s) 41, 295, 352
Hsp92II CATG 4 cut(s) 524, 561, 616, 625
LguI GCTCTTC 1 cut(s) 638
LpnPI CCDG 5 cut(s) 68, 95, 228, 372, 554
LweI GCATC 1 cut(s) 439
MaeI CTAG 5 cut(s) 146, 311, 327, 407, 573
MboII GAAGA 2 cut(s) 644, 655
MhlI GDGCHC 1 cut(s) 650
MluCI AATT 7 cut(s) 16, 160, 168, 196, 236, 436, 465
MlyI GAGTC 2 cut(s) 447, 519
MnlI CCTC 9 cut(s) 42, 69, 186, 592, 595, 609, 620, 622, 628
MslI CAYNNNNRTG 1 cut(s) 389
MspR9I CCNGG 1 cut(s) 83
MvaI CCWGG 1 cut(s) 83
NcoI CCATGG 1 cut(s) 520
NlaIII CATG 4 cut(s) 524, 561, 616, 625
NlaIV GGNNCC 1 cut(s) 80
OliI CACNNNNGTG 1 cut(s) 389
PagI TCATGA 2 cut(s) 612, 621
PciSI GCTCTTC 1 cut(s) 638
PfeI GAWTC 3 cut(s) 394, 503, 536
PkrI GCNGC 1 cut(s) 476
PleI GAGTC 2 cut(s) 447, 519
PpsI GAGTC 2 cut(s) 447, 519
Psp124BI GAGCTC 1 cut(s) 650
Psp6I CCWGG 1 cut(s) 81
PspGI CCWGG 1 cut(s) 81
PspN4I GGNNCC 1 cut(s) 80
PspPI GGNCC 4 cut(s) 173, 317, 479, 607
PsrI GAACNNNNNNTAC 2 cut(s) 107, 139
RsaI GTAC 2 cut(s) 56, 252
RsaNI GTAC 2 cut(s) 55, 251
RseI CAYNNNNRTG 1 cut(s) 389
SacI GAGCTC 1 cut(s) 650
SapI GCTCTTC 1 cut(s) 638
SatI GCNGC 1 cut(s) 475
Sau96I GGNCC 4 cut(s) 173, 317, 479, 607
ScaI AGTACT 1 cut(s) 56
SchI GAGTC 2 cut(s) 447, 519
ScrFI CCNGG 1 cut(s) 83
SduI GDGCHC 1 cut(s) 650
SetI ASST 9 cut(s) 84, 178, 353, 606, 612, 620, 639, 650, 664
SfaNI GCATC 1 cut(s) 439
SinI GGWCC 3 cut(s) 173, 479, 607
SmiMI CAYNNNNRTG 1 cut(s) 389
SmlI CTYRAG 1 cut(s) 425
SmoI CTYRAG 1 cut(s) 425
Sse9I AATT 7 cut(s) 16, 160, 168, 196, 236, 436, 465
SsiI CCGC 2 cut(s) 474, 477
SspMI CTAG 5 cut(s) 146, 311, 327, 407, 573
SstI GAGCTC 1 cut(s) 650
StyD4I CCNGG 1 cut(s) 81
StyI CCWWGG 2 cut(s) 457, 520
TaaI ACNGT 1 cut(s) 128
TaqI TCGA 1 cut(s) 373
TasI AATT 7 cut(s) 16, 160, 168, 196, 236, 436, 465
TatI WGTACW 1 cut(s) 54
TauI GCSGC 1 cut(s) 477
TfiI GAWTC 3 cut(s) 394, 503, 536
TscAI CASTG 1 cut(s) 391
TspDTI ATGAA 3 cut(s) 97, 114, 610
TspRI CASTG 1 cut(s) 391
VpaK11BI GGWCC 3 cut(s) 173, 479, 607
XapI RAATTY 2 cut(s) 436, 465
XbaI TCTAGA 2 cut(s) 310, 406
XcmI CCANNNNNNNNNTGG 2 cut(s) 527, 601
XmiI GTMKAC 1 cut(s) 213
XspI CTAG 5 cut(s) 146, 311, 327, 407, 573
ZrmI AGTACT 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.