Rh2BG257100

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0033033
Physical Location & Seq
Reverse (-)
27046462 .. 27078707
32246 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG257100.1

Sequence Viewer

Length: 717 bp
ATGGCGCCTTTTAGGCTCCCCCTGTGCAATTTGTTTGTGCTTCCAAAGATATCAAGACTTTGTGGTGAAAATGTTTTGCACAAGAGGACATCATTGCCTGAACATGATTATGGGGTTTTGTTTATGCTACTAAGTATGAGGAAGTACTTGCTCAAAGCCGTATGGAGGCACCTGGGATTTGCCGATGGTGCTGTTGGTAATTTGAAAGCTGCTACAGACATAGATGTATCATACAACCAATTATCTGGGAGTTTACCAAGTAGCATTGGGAGTTTAGTGAGTTTGGTCAATCTCTCCTTAGCACATAACAATCTAGAAGGCCCTATTCCTAGTTCCTTTGCTACCTGCTTAAGCTTAGAACTGTTGGATTTATCTGGAAATAGTTTCACTGGAGTGATTCCAAAGTCTCTAGAAGCACTCTCATGTCTCAAGCATCTGAATTTGTCTTTCAACAGACTCCAAGGAGAAATTCCAAGCGGCGGTCCTTTCAAAAACTTCTCTGCTGAATCATTTGTTTCAAACCATGGACTCTGTGGTGGATCCCAATTTCAAGTTCCACCATGCAAAAGAAAAACTAGCATATCTATCTTGAAGGTTTTGCCTCTTTCCTGTGTCTTTTGTCTCTTTGCTGTGTCTTTGCTGATTGAGATTCAGATGGGTGGGGAATTACTGAGTTTCAGGAGGCCAAAGAAGGCTAAAGCTGGAGAGGAGAAATAA

Protein Analysis

238

Amino Acids

25.82

Weight (kDa)

9.16

Isoelectric Point (pI)

37.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 110 - 153 3e-08 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0033033)

Species Orthologous Gene IDs
rosa_samantha Rh2BG256700 Rh2BG257100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 353
AccB1I GGYRCC 2 cut(s) 4, 168
AciI CCGC 2 cut(s) 477, 480
AclWI GGATC 2 cut(s) 534, 547
AcsI RAATTY 2 cut(s) 439, 468
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 146
AfiI CCNNNNNNNGG 2 cut(s) 165, 479
AflII CTTAAG 1 cut(s) 349
AgsI TTSAA 6 cut(s) 205, 451, 490, 519, 551, 592
AjnI CCWGG 1 cut(s) 171
AjuI GAANNNNNNNTTGG 4 cut(s) 453, 485, 537, 569
AleI CACNNNNGTG 1 cut(s) 392
AluBI AGCT 3 cut(s) 209, 354, 701
AluI AGCT 3 cut(s) 209, 354, 701
Alw26I GTCTC 3 cut(s) 411, 431, 626
AlwI GGATC 2 cut(s) 534, 547
AoxI GGCC 2 cut(s) 319, 683
ApeKI GCWGC 1 cut(s) 209
ApoI RAATTY 2 cut(s) 439, 468
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 2 cut(s) 320, 482
AsuHPI GGTGA 1 cut(s) 77
AvaII GGWCC 1 cut(s) 482
BamHI GGATCC 1 cut(s) 539
BanI GGYRCC 2 cut(s) 4, 168
BbvI GCAGC 1 cut(s) 196
BccI CCATC 2 cut(s) 179, 649
BceAI ACGGC 1 cut(s) 143
BciT130I CCWGG 1 cut(s) 173
BcoDI GTCTC 3 cut(s) 411, 431, 626
BfaI CTAG 4 cut(s) 314, 330, 410, 576
BfmI CTRYAG 1 cut(s) 213
BfoI RGCGCY 1 cut(s) 8
BfrI CTTAAG 1 cut(s) 349
BfuAI ACCTGC 1 cut(s) 353
BglI GCCNNNNNGGC 1 cut(s) 13
BisI GCNGC 2 cut(s) 210, 478
BlsI GCNGC 2 cut(s) 211, 479
BmcAI AGTACT 1 cut(s) 146
Bme1390I CCNGG 1 cut(s) 173
Bme18I GGWCC 1 cut(s) 482
BmgT120I GGNCC 2 cut(s) 320, 482
BmiI GGNNCC 4 cut(s) 6, 17, 170, 541
BmrFI CCNGG 1 cut(s) 173
BmsI GCATC 1 cut(s) 442
BpmI CTGGAG 1 cut(s) 411
Bpu10I CCTNAGC 1 cut(s) 298
BpuEI CTTGAG 1 cut(s) 413
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 172, 460, 523
Bsc4I CCNNNNNNNGG 2 cut(s) 165, 479
Bse1I ACTGG 1 cut(s) 394
Bse3DI GCAATG 1 cut(s) 92
BseBI CCWGG 1 cut(s) 173
BseDI CCNNGG 3 cut(s) 172, 460, 523
BseLI CCNNNNNNNGG 2 cut(s) 165, 479
BseMI GCAATG 1 cut(s) 92
BseMII CTCAG 1 cut(s) 662
BseNI ACTGG 1 cut(s) 394
BseXI GCAGC 1 cut(s) 196
BshFI GGCC 2 cut(s) 321, 685
BshNI GGYRCC 2 cut(s) 4, 168
BslI CCNNNNNNNGG 2 cut(s) 165, 479
BsmAI GTCTC 3 cut(s) 411, 431, 626
BsnI GGCC 2 cut(s) 321, 685
Bsp143I GATC 1 cut(s) 539
Bsp19I CCATGG 1 cut(s) 523
BspACI CCGC 2 cut(s) 477, 480
BspANI GGCC 2 cut(s) 321, 685
BspCNI CTCAG 1 cut(s) 663
BspLI GGNNCC 4 cut(s) 6, 17, 170, 541
BspMI ACCTGC 1 cut(s) 353
BspPI GGATC 2 cut(s) 534, 547
BspT107I GGYRCC 2 cut(s) 4, 168
BspTI CTTAAG 1 cut(s) 349
BsrDI GCAATG 1 cut(s) 92
BsrI ACTGG 1 cut(s) 394
BssECI CCNNGG 3 cut(s) 172, 460, 523
BssMI GATC 1 cut(s) 539
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 460, 523
Bst2UI CCWGG 1 cut(s) 173
Bst4CI ACNGT 1 cut(s) 363
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 349
BstDEI CTNAG 4 cut(s) 131, 298, 355, 671
BstDSI CCRYGG 1 cut(s) 523
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 1 cut(s) 542
BstMAI GTCTC 3 cut(s) 411, 431, 626
BstMBI GATC 1 cut(s) 539
BstMWI GCNNNNNNNGC 2 cut(s) 13, 188
BstNI CCWGG 1 cut(s) 173
BstSCI CCNGG 1 cut(s) 171
BstSFI CTRYAG 1 cut(s) 213
BstV1I GCAGC 1 cut(s) 196
BstX2I RGATCY 1 cut(s) 539
BstXI CCANNNNNNTGG 1 cut(s) 245
BstYI RGATCY 1 cut(s) 539
BsuRI GGCC 2 cut(s) 321, 685
BtgI CCRYGG 1 cut(s) 523
BtsIMutI CAGTG 1 cut(s) 387
BveI ACCTGC 1 cut(s) 353
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 2 cut(s) 320, 482
Csp6I GTAC 1 cut(s) 145
CviAII CATG 4 cut(s) 104, 423, 524, 561
CviJI RGCY 8 cut(s) 16, 158, 209, 321, 354, 685, 695, 701
CviKI_1 RGCY 8 cut(s) 16, 158, 209, 321, 354, 685, 695, 701
CviQI GTAC 1 cut(s) 145
DdeI CTNAG 4 cut(s) 131, 298, 355, 671
DinI GGCGCC 1 cut(s) 6
DpnI GATC 1 cut(s) 541
DpnII GATC 1 cut(s) 539
Eco130I CCWWGG 2 cut(s) 460, 523
Eco32I GATATC 1 cut(s) 51
Eco47I GGWCC 1 cut(s) 482
EcoO109I RGGNCCY 1 cut(s) 320
EcoRII CCWGG 1 cut(s) 171
EcoRV GATATC 1 cut(s) 51
EcoT14I CCWWGG 2 cut(s) 460, 523
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 460, 523
FaeI CATG 4 cut(s) 107, 426, 527, 564
FatI CATG 4 cut(s) 103, 422, 523, 560
Fnu4HI GCNGC 2 cut(s) 210, 478
Fsp4HI GCNGC 2 cut(s) 210, 478
FspBI CTAG 4 cut(s) 314, 330, 410, 576
GlaI GCGC 1 cut(s) 6
GluI GCNGC 2 cut(s) 210, 478
GsuI CTGGAG 1 cut(s) 411
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 321, 685
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 4 cut(s) 107, 426, 527, 564
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HindIII AAGCTT 1 cut(s) 352
HinfI GANTC 5 cut(s) 397, 456, 506, 528, 649
HphI GGTGA 1 cut(s) 77
Hpy166II GTNNAC 1 cut(s) 254
Hpy188I TCNGA 2 cut(s) 438, 654
Hpy188III TCNNGA 6 cut(s) 54, 314, 375, 410, 589, 679
Hpy8I GTNNAC 1 cut(s) 254
HpyAV CCTTC 3 cut(s) 311, 586, 685
HpyCH4III ACNGT 1 cut(s) 363
HpyCH4V TGCA 3 cut(s) 27, 79, 564
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 188
HpyF3I CTNAG 4 cut(s) 131, 298, 355, 671
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 4 cut(s) 107, 426, 527, 564
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 1 cut(s) 539
LmnI GCTCC 1 cut(s) 21
Lsp1109I GCAGC 1 cut(s) 196
LweI GCATC 1 cut(s) 442
MaeI CTAG 4 cut(s) 314, 330, 410, 576
MalI GATC 1 cut(s) 541
MboI GATC 1 cut(s) 539
MflI RGATCY 1 cut(s) 539
MluCI AATT 7 cut(s) 28, 199, 239, 439, 468, 545, 665
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 2 cut(s) 450, 522
MmeI TCCRAC 1 cut(s) 345
MnlI CCTC 6 cut(s) 78, 132, 159, 612, 675, 700
MseI TTAA 1 cut(s) 350
MslI CAYNNNNRTG 3 cut(s) 108, 392, 421
MspCI CTTAAG 1 cut(s) 349
MspR9I CCNGG 1 cut(s) 173
MvaI CCWGG 1 cut(s) 173
MwoI GCNNNNNNNGC 2 cut(s) 13, 188
NarI GGCGCC 1 cut(s) 5
NcoI CCATGG 1 cut(s) 523
NdeII GATC 1 cut(s) 539
NlaIII CATG 4 cut(s) 107, 426, 527, 564
NlaIV GGNNCC 4 cut(s) 6, 17, 170, 541
OliI CACNNNNGTG 1 cut(s) 392
PfeI GAWTC 3 cut(s) 397, 506, 649
PkrI GCNGC 2 cut(s) 211, 479
PleI GAGTC 2 cut(s) 450, 522
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 2 cut(s) 450, 522
Psp6I CCWGG 1 cut(s) 171
PspGI CCWGG 1 cut(s) 171
PspN4I GGNNCC 4 cut(s) 6, 17, 170, 541
PspPI GGNCC 2 cut(s) 320, 482
PsuI RGATCY 1 cut(s) 539
RsaI GTAC 1 cut(s) 146
RsaNI GTAC 1 cut(s) 145
RseI CAYNNNNRTG 3 cut(s) 108, 392, 421
SaqAI TTAA 1 cut(s) 350
SatI GCNGC 2 cut(s) 210, 478
Sau3AI GATC 1 cut(s) 539
Sau96I GGNCC 2 cut(s) 320, 482
ScaI AGTACT 1 cut(s) 146
SchI GAGTC 2 cut(s) 450, 522
ScrFI CCNGG 1 cut(s) 173
SetI ASST 6 cut(s) 174, 211, 347, 356, 597, 703
SfaNI GCATC 1 cut(s) 442
SfcI CTRYAG 1 cut(s) 213
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 1 cut(s) 482
SmiMI CAYNNNNRTG 3 cut(s) 108, 392, 421
SmlI CTYRAG 2 cut(s) 349, 428
SmoI CTYRAG 2 cut(s) 349, 428
Sse9I AATT 7 cut(s) 28, 199, 239, 439, 468, 545, 665
SsiI CCGC 2 cut(s) 477, 480
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 4 cut(s) 314, 330, 410, 576
StyD4I CCNGG 1 cut(s) 171
StyI CCWWGG 2 cut(s) 460, 523
TaaI ACNGT 1 cut(s) 363
TasI AATT 7 cut(s) 28, 199, 239, 439, 468, 545, 665
TatI WGTACW 1 cut(s) 144
TauI GCSGC 1 cut(s) 480
TfiI GAWTC 3 cut(s) 397, 506, 649
Tru1I TTAA 1 cut(s) 350
Tru9I TTAA 1 cut(s) 350
TscAI CASTG 1 cut(s) 394
TseI GCWGC 1 cut(s) 209
TspRI CASTG 1 cut(s) 394
Vha464I CTTAAG 1 cut(s) 349
VpaK11BI GGWCC 1 cut(s) 482
XapI RAATTY 2 cut(s) 439, 468
XbaI TCTAGA 2 cut(s) 313, 409
XcmI CCANNNNNNNNNTGG 1 cut(s) 530
XspI CTAG 4 cut(s) 314, 330, 410, 576
ZrmI AGTACT 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.