Rh4AG141900

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0031086
Physical Location & Seq
Forward (+)
36010343 .. 36014438
4096 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG141900.1

Sequence Viewer

Length: 663 bp
ATGGGTTTTCTCAGTAAAGTCTCCGAGCTCCGTGCTAGGGCTCGGAGTCATAAAAAGCTCAAGAAGAACAAGCAGCTACAGACTGTGGAGATAAAAGTGAAGATGGACTGCGAAGGTTGCGAGAGAAGAGTGAAGAAATCAGTGGAAGGCATGAAAGGAGTCACAAGTGTTGAAGTGGATCCCAAGCAAAGCAAGCTCACTGTTATTGGCTATGTGGACCCCAACAAGGTGTTGCATCGTGTCAGGCATCGAACGGGGAAGAAGGCGGATCTGTGGCCGTATGTACCATACGACGTCGTCGAGCATCCATGGGCTCCCGGGGCTTATGATAGGAAGGCACCACCTGGGTACGTGGGGAACTTGCTGAATGACCCTGAGTCTAGACCTAAATCTAGTGCTTTTCTTACCCATGCAGGAGCCTGTGAAGGTTCACTTGCCTGTTCAACATGTCATGTGATTTTAATGGACGTGGAATATTACAACAAATTAGAAGACCCAACTGATGAGGAAAATGATATGTTGGACTTGGCATTTGGACTTACAGAAACGTCTCGTCTGGGTTGCCAAGTCATTGCAAGCCCTGAACTTGATGGAGTTCGTTTAGCTATTCCTGCTGCCACCCGAAATTTTGCTGTTGATGGCTATGTACCAAAACCACACTAG

Protein Analysis

220

Amino Acids

24.46

Weight (kDa)

8.65

Isoelectric Point (pI)

43.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 32 - 87 1.3e-13 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0031086)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000600.1_g000015
rosa_samantha Rh4AG141900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 297
AccB1I GGYRCC 1 cut(s) 337
AciI CCGC 1 cut(s) 266
AclWI GGATC 3 cut(s) 173, 186, 276
AcoI YGGCCR 1 cut(s) 275
AcsI RAATTY 1 cut(s) 625
AcyI GRCGYC 1 cut(s) 294
AfaI GTAC 3 cut(s) 285, 350, 648
AfiI CCNNNNNNNGG 2 cut(s) 37, 226
AflIII ACRYGT 1 cut(s) 446
AgsI TTSAA 2 cut(s) 173, 444
AhdI GACNNNNNGTC 1 cut(s) 376
AjiI CACGTC 1 cut(s) 469
AjnI CCWGG 1 cut(s) 343
AluBI AGCT 5 cut(s) 28, 58, 76, 196, 605
AluI AGCT 5 cut(s) 28, 58, 76, 196, 605
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 2 cut(s) 25, 555
AlwI GGATC 3 cut(s) 173, 186, 276
Ama87I CYCGRG 1 cut(s) 317
AoxI GGCC 1 cut(s) 275
ApeKI GCWGC 2 cut(s) 73, 614
ApoI RAATTY 1 cut(s) 625
ArsI GACNNNNNNTTYG 2 cut(s) 515, 547
AspS9I GGNCC 1 cut(s) 217
AsuC2I CCSGG 2 cut(s) 318, 319
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 1 cut(s) 217
BamHI GGATCC 1 cut(s) 178
BanI GGYRCC 1 cut(s) 337
BanII GRGCYC 3 cut(s) 30, 43, 316
BbsI GAAGAC 1 cut(s) 498
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 2 cut(s) 85, 601
BccI CCATC 3 cut(s) 97, 584, 632
BceAI ACGGC 1 cut(s) 262
BcgI CGANNNNNNTGC 2 cut(s) 14, 48
BciT130I CCWGG 1 cut(s) 345
BcnI CCSGG 2 cut(s) 318, 319
BcoDI GTCTC 2 cut(s) 25, 555
BfaI CTAG 4 cut(s) 36, 381, 393, 661
BfmI CTRYAG 1 cut(s) 77
BisI GCNGC 2 cut(s) 74, 615
BlsI GCNGC 2 cut(s) 75, 616
Bme1390I CCNGG 3 cut(s) 318, 319, 345
Bme18I GGWCC 1 cut(s) 217
BmeRI GACNNNNNGTC 1 cut(s) 376
BmeT110I CYCGRG 1 cut(s) 317
BmgBI CACGTC 1 cut(s) 469
BmgT120I GGNCC 1 cut(s) 217
BmiI GGNNCC 5 cut(s) 180, 219, 315, 339, 418
BmrFI CCNGG 3 cut(s) 318, 319, 345
BmsI GCATC 3 cut(s) 244, 256, 313
BpiI GAAGAC 1 cut(s) 498
BpuEI CTTGAG 1 cut(s) 44
BpuMI CCSGG 2 cut(s) 318, 319
BsaAI YACGTR 1 cut(s) 352
BsaHI GRCGYC 1 cut(s) 294
BsaJI CCNNGG 4 cut(s) 308, 317, 318, 344
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 226
Bse3DI GCAATG 1 cut(s) 570
BseBI CCWGG 1 cut(s) 345
BseDI CCNNGG 4 cut(s) 308, 317, 318, 344
BseGI GGATG 1 cut(s) 304
BseLI CCNNNNNNNGG 2 cut(s) 37, 226
BseMI GCAATG 1 cut(s) 570
BseMII CTCAG 2 cut(s) 25, 366
BseXI GCAGC 2 cut(s) 85, 601
BshFI GGCC 1 cut(s) 277
BshNI GGYRCC 1 cut(s) 337
BsiHKAI GWGCWC 1 cut(s) 30
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 1 cut(s) 318
BslI CCNNNNNNNGG 2 cut(s) 37, 226
BsmAI GTCTC 2 cut(s) 25, 555
BsmBI CGTCTC 1 cut(s) 555
BsnI GGCC 1 cut(s) 277
BsoBI CYCGRG 1 cut(s) 317
Bsp1286I GDGCHC 3 cut(s) 30, 43, 316
Bsp143I GATC 2 cut(s) 178, 268
Bsp19I CCATGG 1 cut(s) 308
BspACI CCGC 1 cut(s) 266
BspANI GGCC 1 cut(s) 277
BspCNI CTCAG 2 cut(s) 24, 367
BspLI GGNNCC 5 cut(s) 180, 219, 315, 339, 418
BspPI GGATC 3 cut(s) 173, 186, 276
BspT107I GGYRCC 1 cut(s) 337
BsrDI GCAATG 1 cut(s) 570
BssECI CCNNGG 4 cut(s) 308, 317, 318, 344
BssMI GATC 2 cut(s) 178, 268
BssNI GRCGYC 1 cut(s) 294
BssT1I CCWWGG 1 cut(s) 308
Bst2UI CCWGG 1 cut(s) 345
Bst4CI ACNGT 2 cut(s) 85, 202
Bst6I CTCTTC 1 cut(s) 121
BstACI GRCGYC 1 cut(s) 294
BstBAI YACGTR 1 cut(s) 352
BstC8I GCNNGC 2 cut(s) 194, 577
BstDEI CTNAG 2 cut(s) 11, 375
BstDSI CCRYGG 1 cut(s) 308
BstF5I GGATG 1 cut(s) 304
BstKTI GATC 2 cut(s) 181, 271
BstMAI GTCTC 2 cut(s) 25, 555
BstMBI GATC 2 cut(s) 178, 268
BstMWI GCNNNNNNNGC 4 cut(s) 117, 193, 320, 611
BstNI CCWGG 1 cut(s) 345
BstNSI RCATGY 1 cut(s) 450
BstSCI CCNGG 3 cut(s) 316, 317, 343
BstSFI CTRYAG 1 cut(s) 77
BstV1I GCAGC 2 cut(s) 85, 601
BstV2I GAAGAC 1 cut(s) 498
BstX2I RGATCY 2 cut(s) 178, 268
BstYI RGATCY 2 cut(s) 178, 268
BsuRI GGCC 1 cut(s) 277
BtgI CCRYGG 1 cut(s) 308
BtrI CACGTC 1 cut(s) 469
BtsCI GGATG 1 cut(s) 304
BtsIMutI CAGTG 2 cut(s) 147, 198
Cac8I GCNNGC 2 cut(s) 194, 577
Cfr13I GGNCC 1 cut(s) 217
Cfr9I CCCGGG 1 cut(s) 317
Csp6I GTAC 3 cut(s) 284, 349, 647
CviAII CATG 5 cut(s) 151, 309, 410, 447, 452
CviQI GTAC 3 cut(s) 284, 349, 647
DdeI CTNAG 2 cut(s) 11, 375
DpnI GATC 2 cut(s) 180, 270
DpnII GATC 2 cut(s) 178, 268
DriI GACNNNNNGTC 1 cut(s) 376
EaeI YGGCCR 1 cut(s) 275
Eam1104I CTCTTC 1 cut(s) 121
Eam1105I GACNNNNNGTC 1 cut(s) 376
EarI CTCTTC 1 cut(s) 121
EciI GGCGGA 1 cut(s) 281
Ecl136II GAGCTC 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 308
Eco24I GRGCYC 3 cut(s) 30, 43, 316
Eco47I GGWCC 1 cut(s) 217
Eco53kI GAGCTC 1 cut(s) 28
Eco88I CYCGRG 1 cut(s) 317
EcoICRI GAGCTC 1 cut(s) 28
EcoRII CCWGG 1 cut(s) 343
EcoT14I CCWWGG 1 cut(s) 308
EcoT38I GRGCYC 3 cut(s) 30, 43, 316
ErhI CCWWGG 1 cut(s) 308
Esp3I CGTCTC 1 cut(s) 555
FaeI CATG 5 cut(s) 154, 312, 413, 450, 455
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FatI CATG 5 cut(s) 150, 308, 409, 446, 451
Fnu4HI GCNGC 2 cut(s) 74, 615
FokI GGATG 1 cut(s) 291
FriOI GRGCYC 3 cut(s) 30, 43, 316
Fsp4HI GCNGC 2 cut(s) 74, 615
FspBI CTAG 4 cut(s) 36, 381, 393, 661
GluI GCNGC 2 cut(s) 74, 615
HaeIII GGCC 1 cut(s) 277
HapII CCGG 1 cut(s) 318
Hin1I GRCGYC 1 cut(s) 294
Hin1II CATG 5 cut(s) 154, 312, 413, 450, 455
HinfI GANTC 3 cut(s) 46, 159, 377
HpaII CCGG 1 cut(s) 318
Hpy166II GTNNAC 2 cut(s) 217, 431
Hpy188I TCNGA 2 cut(s) 25, 45
Hpy188III TCNNGA 2 cut(s) 61, 381
Hpy8I GTNNAC 2 cut(s) 217, 431
Hpy99I CGWCG 3 cut(s) 296, 299, 302
HpyAV CCTTC 5 cut(s) 107, 140, 256, 328, 419
HpyCH4III ACNGT 2 cut(s) 85, 202
HpyCH4IV ACGT 4 cut(s) 294, 351, 468, 548
HpyCH4V TGCA 3 cut(s) 235, 413, 575
HpyF10VI GCNNNNNNNGC 4 cut(s) 117, 193, 320, 611
HpyF3I CTNAG 2 cut(s) 11, 375
HpySE526I ACGT 4 cut(s) 294, 351, 468, 548
Hsp92I GRCGYC 1 cut(s) 294
Hsp92II CATG 5 cut(s) 154, 312, 413, 450, 455
Kzo9I GATC 2 cut(s) 178, 268
LmnI GCTCC 3 cut(s) 33, 319, 416
Lsp1109I GCAGC 2 cut(s) 85, 601
LweI GCATC 3 cut(s) 244, 256, 313
MaeI CTAG 4 cut(s) 36, 381, 393, 661
MaeII ACGT 4 cut(s) 294, 351, 468, 548
MaeIII GTNAC 1 cut(s) 160
MalI GATC 2 cut(s) 180, 270
MboI GATC 2 cut(s) 178, 268
MboII GAAGA 6 cut(s) 76, 112, 138, 145, 271, 503
MflI RGATCY 2 cut(s) 178, 268
MhlI GDGCHC 3 cut(s) 30, 43, 316
MluCI AATT 2 cut(s) 485, 625
MlyI GAGTC 3 cut(s) 55, 168, 386
MmeI TCCRAC 1 cut(s) 501
MnlI CCTC 1 cut(s) 499
MseI TTAA 1 cut(s) 461
MspI CCGG 1 cut(s) 318
MspR9I CCNGG 3 cut(s) 318, 319, 345
MvaI CCWGG 1 cut(s) 345
MwoI GCNNNNNNNGC 4 cut(s) 117, 193, 320, 611
NciI CCSGG 2 cut(s) 318, 319
NcoI CCATGG 1 cut(s) 308
NdeII GATC 2 cut(s) 178, 268
NlaIII CATG 5 cut(s) 154, 312, 413, 450, 455
NlaIV GGNNCC 5 cut(s) 180, 219, 315, 339, 418
NmuCI GTSAC 1 cut(s) 160
NspI RCATGY 1 cut(s) 450
PciI ACATGT 1 cut(s) 446
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PflFI GACNNNGTC 1 cut(s) 296
PkrI GCNGC 2 cut(s) 75, 616
PleI GAGTC 3 cut(s) 54, 167, 385
PpsI GAGTC 3 cut(s) 54, 167, 385
Ppu21I YACGTR 1 cut(s) 352
PscI ACATGT 1 cut(s) 446
Psp124BI GAGCTC 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 343
PspGI CCWGG 1 cut(s) 343
PspN4I GGNNCC 5 cut(s) 180, 219, 315, 339, 418
PspPI GGNCC 1 cut(s) 217
PsuI RGATCY 2 cut(s) 178, 268
PsyI GACNNNGTC 1 cut(s) 296
RsaI GTAC 3 cut(s) 285, 350, 648
RsaNI GTAC 3 cut(s) 284, 349, 647
SacI GAGCTC 1 cut(s) 30
SaqAI TTAA 1 cut(s) 461
SatI GCNGC 2 cut(s) 74, 615
Sau3AI GATC 2 cut(s) 178, 268
Sau96I GGNCC 1 cut(s) 217
SchI GAGTC 3 cut(s) 55, 168, 386
ScrFI CCNGG 3 cut(s) 318, 319, 345
SduI GDGCHC 3 cut(s) 30, 43, 316
SfaNI GCATC 3 cut(s) 244, 256, 313
SfcI CTRYAG 1 cut(s) 77
SinI GGWCC 1 cut(s) 217
SmaI CCCGGG 1 cut(s) 319
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
Sse9I AATT 2 cut(s) 485, 625
SsiI CCGC 1 cut(s) 266
SspI AATATT 1 cut(s) 476
SspMI CTAG 4 cut(s) 36, 381, 393, 661
SstI GAGCTC 1 cut(s) 30
StyD4I CCNGG 3 cut(s) 316, 317, 343
StyI CCWWGG 1 cut(s) 308
TaaI ACNGT 2 cut(s) 85, 202
TaiI ACGT 4 cut(s) 297, 354, 471, 551
TaqI TCGA 2 cut(s) 250, 300
TasI AATT 2 cut(s) 485, 625
Tru1I TTAA 1 cut(s) 461
Tru9I TTAA 1 cut(s) 461
TscAI CASTG 2 cut(s) 147, 205
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 2 cut(s) 73, 614
Tsp45I GTSAC 1 cut(s) 160
TspDTI ATGAA 1 cut(s) 167
TspGWI ACGGA 1 cut(s) 20
TspMI CCCGGG 1 cut(s) 317
TspRI CASTG 2 cut(s) 147, 205
Tth111I GACNNNGTC 1 cut(s) 296
VpaK11BI GGWCC 1 cut(s) 217
XapI RAATTY 1 cut(s) 625
XbaI TCTAGA 1 cut(s) 380
XceI RCATGY 1 cut(s) 450
XmaI CCCGGG 1 cut(s) 317
XspI CTAG 4 cut(s) 36, 381, 393, 661
ZraI GACGTC 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.