Rh4AG171000

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0026064
Physical Location & Seq
Forward (+)
43010369 .. 43012645
2277 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG171000.1

Sequence Viewer

Length: 1080 bp
ATGAAAAGTGGCATAAAAGAACTGAAGTCATGGATTGGATGTTGCATTTCCTTGGAAGTTTTGACGTTGAATGAAACTTCAATAAAACAACTGCCCTTAACGATTGGAAATCTTACTTCTTTAAGAGAATTGGACCTGTGTGAAACTCCCCTTAAGGAATTGCCTTCATCGATTGGGTATTGCACTGCCTTGAAGAAATTGGACGCGTCTGAAACTCCCATTGAAGAATTGCCTCTATCAATTAGGTATCTCACTTCTTTAAGAGAATTGAAGCTGTCTAACACTCGCATCAAAGAATTGCCTCTATCAATTGGGGATCTCACGTCCTTGGAGGAATTGAACGTTTCTAAAACTTCCATCAGAGAACTGCCTTCTTCAATTGGGAATCTCACGTCCTTGGTGGAATTGGACGCTTCTAAAACTCTCATCGAAGTATTGCCTTTGTCAATTGGCTATCTCACTTCTTTAAGAGAATTGAAGCTGTCTAAGACTCGCATCAAGGAATTGCCTCTATCAATTGGGGATCTCATGTCCTTGGAGGAATTGAACGTTTCTAAAACTTCCATCAGAGAACTGCCTTCTTCAATTGGGAATCTCATATCCTTGGTGGAATTGGACGTTTCTAAAACTTCCATCAGAGAACTGCCTTCTTCAATTGGGAAACTCACGTCCTTGGTGAAATTGGTCGCTTCTGAAACTCCCATCGAACCTTATTACGATAATGGCAACCGTATTGAATTAAAAGCGACAGAGTCATCAGCTCATGATCATGTATTTGTGCATCTGACATGTATTGCTTTCAAGGAGCTAGAGGTGTTGGATCTTGTGGTTCGACTGTTACCTATTATTTCCTGCCGCCCTGTCAGAGGGTTGCTCCTAAAAAGTTTCGGGGTCCACCTGGCTAATATGAAAGAGGATCATGCAACTGGTGAAGACCTGGCACGAGAAAGATATAGGCAGAGCAATGCTAGCATTTCTGATCAGGTGGCTTTGCACGCAGAAGGGGCAGCGGTAGCATCATTAGTATCAGATGACAGCAATTACGGGGAAGTACATGAGGATCATCATAATGGGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

359

Amino Acids

39.47

Weight (kDa)

5.29

Isoelectric Point (pI)

32.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 12 - 92 3.4e-12 Leucine-rich repeat region
LRR_14 PF23598 81 - 162 4e-17 Leucine-rich repeat region
LRR_4 PF12799 85 - 123 1.9e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 150 - 229 1.8e-17 Leucine-rich repeat region
LRR_14 PF23598 173 - 233 5.1e-13 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0026064)

Species Orthologous Gene IDs
rosa_samantha Rh4AG170700 Rh4AG171000 Rh4CG181800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 206
AciI CCGC 2 cut(s) 856, 1010
AclI AACGTT 2 cut(s) 342, 549
AclWI GGATC 5 cut(s) 324, 531, 828, 924, 1068
AcuI CTGAAG 1 cut(s) 44
AfaI GTAC 1 cut(s) 1053
AfiI CCNNNNNNNGG 1 cut(s) 866
AflII CTTAAG 1 cut(s) 152
AflIII ACRYGT 2 cut(s) 204, 788
AjiI CACGTC 3 cut(s) 324, 393, 669
AjnI CCWGG 2 cut(s) 897, 936
AluBI AGCT 4 cut(s) 274, 481, 761, 808
AluI AGCT 4 cut(s) 274, 481, 761, 808
AlwI GGATC 5 cut(s) 324, 531, 828, 924, 1068
ApeKI GCWGC 1 cut(s) 1007
AspS9I GGNCC 2 cut(s) 133, 892
AsuHPI GGTGA 2 cut(s) 688, 941
AsuNHI GCTAGC 1 cut(s) 968
AvaII GGWCC 2 cut(s) 133, 892
BauI CACGAG 1 cut(s) 942
BbsI GAAGAC 1 cut(s) 939
BbvI GCAGC 1 cut(s) 1019
BccI CCATC 4 cut(s) 365, 572, 641, 710
BciT130I CCWGG 2 cut(s) 899, 938
BclI TGATCA 2 cut(s) 766, 979
BfaI CTAG 2 cut(s) 809, 969
BfrI CTTAAG 1 cut(s) 152
BisI GCNGC 2 cut(s) 856, 1008
BlsI GCNGC 2 cut(s) 857, 1009
Bme1390I CCNGG 2 cut(s) 899, 938
Bme18I GGWCC 2 cut(s) 133, 892
BmgBI CACGTC 3 cut(s) 324, 393, 669
BmgT120I GGNCC 2 cut(s) 133, 892
BmiI GGNNCC 1 cut(s) 893
BmrFI CCNGG 2 cut(s) 899, 938
BmsI GCATC 4 cut(s) 297, 504, 790, 1025
BmtI GCTAGC 1 cut(s) 972
BpiI GAAGAC 1 cut(s) 939
BplI GAGNNNNNCTC 2 cut(s) 858, 890
Bsa29I ATCGAT 1 cut(s) 170
BsaJI CCNNGG 6 cut(s) 51, 327, 396, 534, 603, 672
Bsc4I CCNNNNNNNGG 1 cut(s) 866
Bse1I ACTGG 1 cut(s) 931
Bse3DI GCAATG 1 cut(s) 970
BseBI CCWGG 2 cut(s) 899, 938
BseCI ATCGAT 1 cut(s) 170
BseDI CCNNGG 6 cut(s) 51, 327, 396, 534, 603, 672
BseGI GGATG 1 cut(s) 44
BseLI CCNNNNNNNGG 1 cut(s) 866
BseMI GCAATG 1 cut(s) 970
BseNI ACTGG 1 cut(s) 931
BseXI GCAGC 1 cut(s) 1019
Bsh1236I CGCG 1 cut(s) 206
BshVI ATCGAT 1 cut(s) 170
BslI CCNNNNNNNGG 1 cut(s) 866
Bsp143I GATC 7 cut(s) 316, 523, 766, 820, 916, 979, 1060
BspACI CCGC 2 cut(s) 856, 1010
BspDI ATCGAT 1 cut(s) 170
BspFNI CGCG 1 cut(s) 206
BspHI TCATGA 1 cut(s) 763
BspLI GGNNCC 1 cut(s) 893
BspOI GCTAGC 1 cut(s) 972
BspPI GGATC 5 cut(s) 324, 531, 828, 924, 1068
BspTI CTTAAG 1 cut(s) 152
BsrDI GCAATG 1 cut(s) 970
BsrI ACTGG 1 cut(s) 931
BssECI CCNNGG 6 cut(s) 51, 327, 396, 534, 603, 672
BssMI GATC 7 cut(s) 316, 523, 766, 820, 916, 979, 1060
BssSI CACGAG 1 cut(s) 942
BssT1I CCWWGG 6 cut(s) 51, 327, 396, 534, 603, 672
Bst2BI CACGAG 1 cut(s) 942
Bst2UI CCWGG 2 cut(s) 899, 938
Bst4CI ACNGT 2 cut(s) 731, 837
BstAFI CTTAAG 1 cut(s) 152
BstC8I GCNNGC 2 cut(s) 970, 996
BstDEI CTNAG 1 cut(s) 486
BstENI CCTNNNNNAGG 1 cut(s) 864
BstF5I GGATG 1 cut(s) 44
BstFNI CGCG 1 cut(s) 206
BstKTI GATC 7 cut(s) 319, 526, 769, 823, 919, 982, 1063
BstMBI GATC 7 cut(s) 316, 523, 766, 820, 916, 979, 1060
BstMWI GCNNNNNNNGC 4 cut(s) 969, 995, 1004, 1013
BstNI CCWGG 2 cut(s) 899, 938
BstNSI RCATGY 1 cut(s) 792
BstSCI CCNGG 2 cut(s) 897, 936
BstUI CGCG 1 cut(s) 206
BstV1I GCAGC 1 cut(s) 1019
BstV2I GAAGAC 1 cut(s) 939
BstX2I RGATCY 3 cut(s) 316, 523, 820
BstYI RGATCY 3 cut(s) 316, 523, 820
Bsu15I ATCGAT 1 cut(s) 170
BsuTUI ATCGAT 1 cut(s) 170
BtrI CACGTC 3 cut(s) 324, 393, 669
BtsCI GGATG 1 cut(s) 44
BtsI GCAGTG 1 cut(s) 183
BtsIMutI CAGTG 1 cut(s) 183
Cac8I GCNNGC 2 cut(s) 970, 996
CciI TCATGA 1 cut(s) 763
Cfr13I GGNCC 2 cut(s) 133, 892
ClaI ATCGAT 1 cut(s) 170
CseI GACGC 3 cut(s) 195, 212, 419
Csp6I GTAC 1 cut(s) 1052
CviAII CATG 7 cut(s) 30, 529, 764, 770, 789, 920, 1055
CviJI RGCY 7 cut(s) 274, 453, 481, 761, 808, 902, 989
CviKI_1 RGCY 7 cut(s) 274, 453, 481, 761, 808, 902, 989
CviQI GTAC 1 cut(s) 1052
DdeI CTNAG 1 cut(s) 486
DpnI GATC 7 cut(s) 318, 525, 768, 822, 918, 981, 1062
DpnII GATC 7 cut(s) 316, 523, 766, 820, 916, 979, 1060
Eco130I CCWWGG 6 cut(s) 51, 327, 396, 534, 603, 672
Eco47I GGWCC 2 cut(s) 133, 892
Eco57I CTGAAG 1 cut(s) 44
EcoNI CCTNNNNNAGG 1 cut(s) 864
EcoRII CCWGG 2 cut(s) 897, 936
EcoT14I CCWWGG 6 cut(s) 51, 327, 396, 534, 603, 672
ErhI CCWWGG 6 cut(s) 51, 327, 396, 534, 603, 672
FaeI CATG 7 cut(s) 33, 532, 767, 773, 792, 923, 1058
FatI CATG 7 cut(s) 29, 528, 763, 769, 788, 919, 1054
FbaI TGATCA 2 cut(s) 766, 979
Fnu4HI GCNGC 2 cut(s) 856, 1008
FokI GGATG 1 cut(s) 51
Fsp4HI GCNGC 2 cut(s) 856, 1008
FspBI CTAG 2 cut(s) 809, 969
GluI GCNGC 2 cut(s) 856, 1008
HgaI GACGC 3 cut(s) 195, 212, 419
Hin1II CATG 7 cut(s) 33, 532, 767, 773, 792, 923, 1058
HinfI GANTC 4 cut(s) 385, 490, 592, 752
HphI GGTGA 2 cut(s) 688, 941
Hpy166II GTNNAC 1 cut(s) 895
Hpy188I TCNGA 9 cut(s) 211, 362, 569, 638, 694, 786, 866, 979, 1030
Hpy188III TCNNGA 1 cut(s) 764
Hpy8I GTNNAC 1 cut(s) 895
HpyAV CCTTC 5 cut(s) 174, 381, 588, 657, 995
HpyCH4III ACNGT 2 cut(s) 731, 837
HpyCH4IV ACGT 7 cut(s) 65, 323, 342, 392, 549, 618, 668
HpyCH4V TGCA 5 cut(s) 45, 183, 781, 923, 994
HpyF10VI GCNNNNNNNGC 4 cut(s) 969, 995, 1004, 1013
HpyF3I CTNAG 1 cut(s) 486
HpySE526I ACGT 7 cut(s) 65, 323, 342, 392, 549, 618, 668
Hsp92II CATG 7 cut(s) 33, 532, 767, 773, 792, 923, 1058
Ksp22I TGATCA 2 cut(s) 766, 979
Kzo9I GATC 7 cut(s) 316, 523, 766, 820, 916, 979, 1060
LmnI GCTCC 2 cut(s) 805, 879
LpnPI CCDG 9 cut(s) 149, 865, 873, 884, 911, 912, 923, 950, 968
Lsp1109I GCAGC 1 cut(s) 1019
LweI GCATC 4 cut(s) 297, 504, 790, 1025
MaeI CTAG 2 cut(s) 809, 969
MaeII ACGT 7 cut(s) 65, 323, 342, 392, 549, 618, 668
MaeIII GTNAC 1 cut(s) 837
MalI GATC 7 cut(s) 318, 525, 768, 822, 918, 981, 1062
MboI GATC 7 cut(s) 316, 523, 766, 820, 916, 979, 1060
MboII GAAGA 6 cut(s) 205, 236, 366, 573, 642, 944
MfeI CAATTG 6 cut(s) 309, 378, 447, 516, 585, 654
MflI RGATCY 3 cut(s) 316, 523, 820
MluI ACGCGT 1 cut(s) 204
MlyI GAGTC 2 cut(s) 484, 761
MmeI TCCRAC 1 cut(s) 798
MnlI CCTC 9 cut(s) 243, 312, 325, 519, 532, 805, 860, 907, 1051
MseI TTAA 6 cut(s) 98, 122, 153, 260, 467, 740
MslI CAYNNNNRTG 2 cut(s) 768, 1068
MspA1I CMGCKG 1 cut(s) 1010
MspCI CTTAAG 1 cut(s) 152
MspR9I CCNGG 2 cut(s) 899, 938
MunI CAATTG 6 cut(s) 309, 378, 447, 516, 585, 654
MvaI CCWGG 2 cut(s) 899, 938
MvnI CGCG 1 cut(s) 206
MwoI GCNNNNNNNGC 4 cut(s) 969, 995, 1004, 1013
NdeII GATC 7 cut(s) 316, 523, 766, 820, 916, 979, 1060
NheI GCTAGC 1 cut(s) 968
NlaIII CATG 7 cut(s) 33, 532, 767, 773, 792, 923, 1058
NlaIV GGNNCC 1 cut(s) 893
NspI RCATGY 1 cut(s) 792
PagI TCATGA 1 cut(s) 763
PciI ACATGT 1 cut(s) 788
PfeI GAWTC 2 cut(s) 385, 592
PflFI GACNNNGTC 1 cut(s) 751
PkrI GCNGC 2 cut(s) 857, 1009
PleI GAGTC 2 cut(s) 484, 760
PpsI GAGTC 2 cut(s) 484, 760
PscI ACATGT 1 cut(s) 788
Psp1406I AACGTT 2 cut(s) 342, 549
Psp6I CCWGG 2 cut(s) 897, 936
PspGI CCWGG 2 cut(s) 897, 936
PspN4I GGNNCC 1 cut(s) 893
PspPI GGNCC 2 cut(s) 133, 892
PsuI RGATCY 3 cut(s) 316, 523, 820
PsyI GACNNNGTC 1 cut(s) 751
RsaI GTAC 1 cut(s) 1053
RsaNI GTAC 1 cut(s) 1052
RseI CAYNNNNRTG 2 cut(s) 768, 1068
SaqAI TTAA 6 cut(s) 98, 122, 153, 260, 467, 740
SatI GCNGC 2 cut(s) 856, 1008
Sau3AI GATC 7 cut(s) 316, 523, 766, 820, 916, 979, 1060
Sau96I GGNCC 2 cut(s) 133, 892
SchI GAGTC 2 cut(s) 484, 761
ScrFI CCNGG 2 cut(s) 899, 938
SfaNI GCATC 4 cut(s) 297, 504, 790, 1025
SinI GGWCC 2 cut(s) 133, 892
SmiMI CAYNNNNRTG 2 cut(s) 768, 1068
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
SsiI CCGC 2 cut(s) 856, 1010
SspMI CTAG 2 cut(s) 809, 969
StyD4I CCNGG 2 cut(s) 897, 936
StyI CCWWGG 6 cut(s) 51, 327, 396, 534, 603, 672
TaaI ACNGT 2 cut(s) 731, 837
TaiI ACGT 7 cut(s) 68, 326, 345, 395, 552, 621, 671
TaqI TCGA 4 cut(s) 170, 429, 705, 832
TatI WGTACW 1 cut(s) 1051
TauI GCSGC 1 cut(s) 858
TfiI GAWTC 2 cut(s) 385, 592
Tru1I TTAA 6 cut(s) 98, 122, 153, 260, 467, 740
Tru9I TTAA 6 cut(s) 98, 122, 153, 260, 467, 740
TscAI CASTG 1 cut(s) 190
TseI GCWGC 1 cut(s) 1007
TspDTI ATGAA 4 cut(s) 17, 87, 156, 923
TspRI CASTG 1 cut(s) 190
Tth111I GACNNNGTC 1 cut(s) 751
Vha464I CTTAAG 1 cut(s) 152
VpaK11BI GGWCC 2 cut(s) 133, 892
XagI CCTNNNNNAGG 1 cut(s) 864
XceI RCATGY 1 cut(s) 792
XspI CTAG 2 cut(s) 809, 969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.