Rh4CG181800

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0026064
Physical Location & Seq
Forward (+)
39291783 .. 39299869
8087 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG181800.1

Sequence Viewer

Length: 984 bp
ATGTTTCATACTATCCTTTTGGACAAGGAGAATGTTGTGTCTTCAATATGGCTGGAAGAGGAATTGCCTTCATCGATTGGGTATTGCACTGCCTTGAAGATATTGGACGCGTCTGAAACTCCCATTGAAGAATTGCCTCTATCAATTAGGTATCTCACTTCTTTAAGAGAATTGAAGCTGTCTAACACTCGCATCAAAGAATTGCCTCTATCAATTGGGGATCTCACGTCCTTGGAGGAATTGAACGTTTCTAAAACTTCCATCAGAGAACTGCCTTCTTCAATTGGGAATCTCACGTCCTTGGTGGAATTGGACGCTTCTAAAACTCTCATCGAAGTATTGCCTTTGTCAATTGGCTATCTCACTTCTTTAAGAGAATTGAAGCTGTCTAAGACTCGCATCAAAGAATTGCCTCTATCAATTGGGGATCTCATGTCCTTGGAGGAATTGAACGTTTCTAAAACTTCCATCAGAGAACTGCCTTCTTCAATTGGGAATCTCACATCCTTGGTGGAATTGGACGTTTCTAAAACTTCCATCAGAGAACTGCCTTCTTCAATTGGGAAACTCACGTCCTTGGTGAAATTGGTCGCTTCTGAAACTCCCATCGAACCTTATTACGATAATGGCAACCGTATTGAATTAAAAGCGACAGAGTCATCAGCTCATGATCATGTATTTGTGCATCTGACATGTATTGCTTTCAAGGAGCTAGAGGTGTTGGATCTTGTGGTTCGACTGTTACCTATTATTTCCTGCCGCCCTGTCAGAGGGTTGCTCCTAAAAAGTTTCGGGGTCCACCTGGCTAATATGAAAGAGGATCATGCAACTGGTGAAGACCTGGCACGAGAAAGATATAGGCAGAGCAATGCTAGCATTTCTGATCAGGTGGCTTTGCACGCAGAAGGGGCAGCGGTAGCATCATTAGTATCAGATGACAGCAATTACGGGGAAGTACACGAGGATCATCATAATGGGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

327

Amino Acids

36.07

Weight (kDa)

5.08

Isoelectric Point (pI)

37.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 49 - 129 1.9e-17 Leucine-rich repeat region
LRR_4 PF12799 53 - 91 2.5e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 118 - 197 3.8e-18 Leucine-rich repeat region
LRR_14 PF23598 141 - 201 2.4e-13 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0026064)

Species Orthologous Gene IDs
rosa_samantha Rh4AG170700 Rh4AG171000 Rh4CG181800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 110
AciI CCGC 2 cut(s) 760, 914
AclI AACGTT 2 cut(s) 246, 453
AclWI GGATC 5 cut(s) 228, 435, 732, 828, 972
AfaI GTAC 1 cut(s) 957
AfiI CCNNNNNNNGG 1 cut(s) 770
AflIII ACRYGT 2 cut(s) 108, 692
AjiI CACGTC 3 cut(s) 228, 297, 573
AjnI CCWGG 2 cut(s) 801, 840
AluBI AGCT 4 cut(s) 178, 385, 665, 712
AluI AGCT 4 cut(s) 178, 385, 665, 712
AlwI GGATC 5 cut(s) 228, 435, 732, 828, 972
ApeKI GCWGC 1 cut(s) 911
AspS9I GGNCC 1 cut(s) 796
AsuHPI GGTGA 2 cut(s) 592, 845
AsuNHI GCTAGC 1 cut(s) 872
AvaII GGWCC 1 cut(s) 796
BauI CACGAG 2 cut(s) 846, 959
BbsI GAAGAC 2 cut(s) 33, 843
BbvI GCAGC 1 cut(s) 923
BccI CCATC 4 cut(s) 269, 476, 545, 614
BciT130I CCWGG 2 cut(s) 803, 842
BclI TGATCA 2 cut(s) 670, 883
BfaI CTAG 2 cut(s) 713, 873
BisI GCNGC 2 cut(s) 760, 912
BlsI GCNGC 2 cut(s) 761, 913
Bme1390I CCNGG 2 cut(s) 803, 842
Bme18I GGWCC 1 cut(s) 796
BmgBI CACGTC 3 cut(s) 228, 297, 573
BmgT120I GGNCC 1 cut(s) 796
BmiI GGNNCC 1 cut(s) 797
BmrFI CCNGG 2 cut(s) 803, 842
BmsI GCATC 4 cut(s) 201, 408, 694, 929
BmtI GCTAGC 1 cut(s) 876
BpiI GAAGAC 2 cut(s) 33, 843
BplI GAGNNNNNCTC 2 cut(s) 762, 794
Bsa29I ATCGAT 1 cut(s) 74
BsaJI CCNNGG 5 cut(s) 231, 300, 438, 507, 576
Bsc4I CCNNNNNNNGG 1 cut(s) 770
Bse1I ACTGG 1 cut(s) 835
Bse3DI GCAATG 1 cut(s) 874
BseBI CCWGG 2 cut(s) 803, 842
BseCI ATCGAT 1 cut(s) 74
BseDI CCNNGG 5 cut(s) 231, 300, 438, 507, 576
BseGI GGATG 1 cut(s) 503
BseLI CCNNNNNNNGG 1 cut(s) 770
BseMI GCAATG 1 cut(s) 874
BseNI ACTGG 1 cut(s) 835
BseXI GCAGC 1 cut(s) 923
Bsh1236I CGCG 1 cut(s) 110
BshVI ATCGAT 1 cut(s) 74
BslI CCNNNNNNNGG 1 cut(s) 770
Bsp143I GATC 7 cut(s) 220, 427, 670, 724, 820, 883, 964
BspACI CCGC 2 cut(s) 760, 914
BspDI ATCGAT 1 cut(s) 74
BspFNI CGCG 1 cut(s) 110
BspHI TCATGA 1 cut(s) 667
BspLI GGNNCC 1 cut(s) 797
BspOI GCTAGC 1 cut(s) 876
BspPI GGATC 5 cut(s) 228, 435, 732, 828, 972
BsrDI GCAATG 1 cut(s) 874
BsrI ACTGG 1 cut(s) 835
BssECI CCNNGG 5 cut(s) 231, 300, 438, 507, 576
BssMI GATC 7 cut(s) 220, 427, 670, 724, 820, 883, 964
BssSI CACGAG 2 cut(s) 846, 959
BssT1I CCWWGG 5 cut(s) 231, 300, 438, 507, 576
Bst2BI CACGAG 2 cut(s) 846, 959
Bst2UI CCWGG 2 cut(s) 803, 842
Bst4CI ACNGT 2 cut(s) 635, 741
Bst6I CTCTTC 1 cut(s) 51
BstC8I GCNNGC 2 cut(s) 874, 900
BstDEI CTNAG 1 cut(s) 390
BstENI CCTNNNNNAGG 1 cut(s) 768
BstF5I GGATG 1 cut(s) 503
BstFNI CGCG 1 cut(s) 110
BstKTI GATC 7 cut(s) 223, 430, 673, 727, 823, 886, 967
BstMBI GATC 7 cut(s) 220, 427, 670, 724, 820, 883, 964
BstMWI GCNNNNNNNGC 4 cut(s) 873, 899, 908, 917
BstNI CCWGG 2 cut(s) 803, 842
BstNSI RCATGY 1 cut(s) 696
BstSCI CCNGG 2 cut(s) 801, 840
BstUI CGCG 1 cut(s) 110
BstV1I GCAGC 1 cut(s) 923
BstV2I GAAGAC 2 cut(s) 33, 843
BstX2I RGATCY 3 cut(s) 220, 427, 724
BstYI RGATCY 3 cut(s) 220, 427, 724
Bsu15I ATCGAT 1 cut(s) 74
BsuTUI ATCGAT 1 cut(s) 74
BtrI CACGTC 3 cut(s) 228, 297, 573
BtsCI GGATG 1 cut(s) 503
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 1 cut(s) 87
Cac8I GCNNGC 2 cut(s) 874, 900
CciI TCATGA 1 cut(s) 667
Cfr13I GGNCC 1 cut(s) 796
ClaI ATCGAT 1 cut(s) 74
CseI GACGC 3 cut(s) 99, 116, 323
Csp6I GTAC 1 cut(s) 956
CviAII CATG 5 cut(s) 433, 668, 674, 693, 824
CviJI RGCY 8 cut(s) 52, 178, 357, 385, 665, 712, 806, 893
CviKI_1 RGCY 8 cut(s) 52, 178, 357, 385, 665, 712, 806, 893
CviQI GTAC 1 cut(s) 956
DdeI CTNAG 1 cut(s) 390
DpnI GATC 7 cut(s) 222, 429, 672, 726, 822, 885, 966
DpnII GATC 7 cut(s) 220, 427, 670, 724, 820, 883, 964
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
Eco130I CCWWGG 5 cut(s) 231, 300, 438, 507, 576
Eco47I GGWCC 1 cut(s) 796
EcoNI CCTNNNNNAGG 1 cut(s) 768
EcoRII CCWGG 2 cut(s) 801, 840
EcoT14I CCWWGG 5 cut(s) 231, 300, 438, 507, 576
ErhI CCWWGG 5 cut(s) 231, 300, 438, 507, 576
FaeI CATG 5 cut(s) 436, 671, 677, 696, 827
FatI CATG 5 cut(s) 432, 667, 673, 692, 823
FbaI TGATCA 2 cut(s) 670, 883
Fnu4HI GCNGC 2 cut(s) 760, 912
FokI GGATG 1 cut(s) 490
Fsp4HI GCNGC 2 cut(s) 760, 912
FspBI CTAG 2 cut(s) 713, 873
GluI GCNGC 2 cut(s) 760, 912
HgaI GACGC 3 cut(s) 99, 116, 323
Hin1II CATG 5 cut(s) 436, 671, 677, 696, 827
HinfI GANTC 4 cut(s) 289, 394, 496, 656
HphI GGTGA 2 cut(s) 592, 845
Hpy166II GTNNAC 2 cut(s) 799, 958
Hpy188I TCNGA 9 cut(s) 115, 266, 473, 542, 598, 690, 770, 883, 934
Hpy188III TCNNGA 1 cut(s) 668
Hpy8I GTNNAC 2 cut(s) 799, 958
HpyAV CCTTC 5 cut(s) 78, 285, 492, 561, 899
HpyCH4III ACNGT 2 cut(s) 635, 741
HpyCH4IV ACGT 6 cut(s) 227, 246, 296, 453, 522, 572
HpyCH4V TGCA 4 cut(s) 87, 685, 827, 898
HpyF10VI GCNNNNNNNGC 4 cut(s) 873, 899, 908, 917
HpyF3I CTNAG 1 cut(s) 390
HpySE526I ACGT 6 cut(s) 227, 246, 296, 453, 522, 572
Hsp92II CATG 5 cut(s) 436, 671, 677, 696, 827
Ksp22I TGATCA 2 cut(s) 670, 883
Kzo9I GATC 7 cut(s) 220, 427, 670, 724, 820, 883, 964
LmnI GCTCC 2 cut(s) 709, 783
LpnPI CCDG 9 cut(s) 38, 769, 777, 788, 815, 816, 827, 854, 872
Lsp1109I GCAGC 1 cut(s) 923
LweI GCATC 4 cut(s) 201, 408, 694, 929
MaeI CTAG 2 cut(s) 713, 873
MaeII ACGT 6 cut(s) 227, 246, 296, 453, 522, 572
MaeIII GTNAC 1 cut(s) 741
MalI GATC 7 cut(s) 222, 429, 672, 726, 822, 885, 966
MboI GATC 7 cut(s) 220, 427, 670, 724, 820, 883, 964
MboII GAAGA 8 cut(s) 33, 68, 109, 140, 270, 477, 546, 848
MfeI CAATTG 6 cut(s) 213, 282, 351, 420, 489, 558
MflI RGATCY 3 cut(s) 220, 427, 724
MluI ACGCGT 1 cut(s) 108
MlyI GAGTC 2 cut(s) 388, 665
MmeI TCCRAC 1 cut(s) 702
MseI TTAA 3 cut(s) 164, 371, 644
MslI CAYNNNNRTG 2 cut(s) 672, 972
MspA1I CMGCKG 1 cut(s) 914
MspR9I CCNGG 2 cut(s) 803, 842
MunI CAATTG 6 cut(s) 213, 282, 351, 420, 489, 558
MvaI CCWGG 2 cut(s) 803, 842
MvnI CGCG 1 cut(s) 110
MwoI GCNNNNNNNGC 4 cut(s) 873, 899, 908, 917
NdeII GATC 7 cut(s) 220, 427, 670, 724, 820, 883, 964
NheI GCTAGC 1 cut(s) 872
NlaIII CATG 5 cut(s) 436, 671, 677, 696, 827
NlaIV GGNNCC 1 cut(s) 797
NspI RCATGY 1 cut(s) 696
PagI TCATGA 1 cut(s) 667
PciI ACATGT 1 cut(s) 692
PfeI GAWTC 2 cut(s) 289, 496
PflFI GACNNNGTC 1 cut(s) 655
PkrI GCNGC 2 cut(s) 761, 913
PleI GAGTC 2 cut(s) 388, 664
PpsI GAGTC 2 cut(s) 388, 664
PscI ACATGT 1 cut(s) 692
Psp1406I AACGTT 2 cut(s) 246, 453
Psp6I CCWGG 2 cut(s) 801, 840
PspGI CCWGG 2 cut(s) 801, 840
PspN4I GGNNCC 1 cut(s) 797
PspPI GGNCC 1 cut(s) 796
PsuI RGATCY 3 cut(s) 220, 427, 724
PsyI GACNNNGTC 1 cut(s) 655
RsaI GTAC 1 cut(s) 957
RsaNI GTAC 1 cut(s) 956
RseI CAYNNNNRTG 2 cut(s) 672, 972
SaqAI TTAA 3 cut(s) 164, 371, 644
SatI GCNGC 2 cut(s) 760, 912
Sau3AI GATC 7 cut(s) 220, 427, 670, 724, 820, 883, 964
Sau96I GGNCC 1 cut(s) 796
SchI GAGTC 2 cut(s) 388, 665
ScrFI CCNGG 2 cut(s) 803, 842
SfaNI GCATC 4 cut(s) 201, 408, 694, 929
SinI GGWCC 1 cut(s) 796
SmiMI CAYNNNNRTG 2 cut(s) 672, 972
SsiI CCGC 2 cut(s) 760, 914
SspMI CTAG 2 cut(s) 713, 873
StyD4I CCNGG 2 cut(s) 801, 840
StyI CCWWGG 5 cut(s) 231, 300, 438, 507, 576
TaaI ACNGT 2 cut(s) 635, 741
TaiI ACGT 6 cut(s) 230, 249, 299, 456, 525, 575
TaqI TCGA 4 cut(s) 74, 333, 609, 736
TatI WGTACW 1 cut(s) 955
TauI GCSGC 1 cut(s) 762
TfiI GAWTC 2 cut(s) 289, 496
Tru1I TTAA 3 cut(s) 164, 371, 644
Tru9I TTAA 3 cut(s) 164, 371, 644
TscAI CASTG 1 cut(s) 94
TseI GCWGC 1 cut(s) 911
TspDTI ATGAA 2 cut(s) 60, 827
TspRI CASTG 1 cut(s) 94
Tth111I GACNNNGTC 1 cut(s) 655
VpaK11BI GGWCC 1 cut(s) 796
XagI CCTNNNNNAGG 1 cut(s) 768
XceI RCATGY 1 cut(s) 696
XspI CTAG 2 cut(s) 713, 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.