Rh4BG196300

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0023086
Physical Location & Seq
Reverse (-)
33858266 .. 33859496
1231 bp
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UTR
Exon/CDS
Intron
Rh4BG196300.1

Sequence Viewer

Length: 243 bp
ATGTCTGTCGGCAGTGGTCTCCAGAACCGCCGCGGCAAATTTGATGTCGACGCGTTACATAATAAGAGGGCTGAGATAAGGCCTGATGAAGCTATCTTTCTGAAGTTTAATAACAAAGTCCCTAGTTTCGGATTGCGGTTGGACAGGACGGATTTGAATCTTGATTATGCAGCCAGATATTCAACTGAGATTCCAGATGTGTACGAGATGTCTCGAGACTGTTTTTCTGGAGTGATGAACTAG

Protein Analysis

80

Amino Acids

9.17

Weight (kDa)

6.55

Isoelectric Point (pI)

55.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G6PD_C PF02781 25 - 70 6.1e-06 Glucose-6-phosphate dehydrogenase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0023086)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002438.1_g000019
rosa_samantha Rh4AG198300 Rh4BG196300 Rh4DG196100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 48
AccII CGCG 2 cut(s) 33, 53
AciI CCGC 4 cut(s) 28, 31, 33, 136
AcsI RAATTY 1 cut(s) 38
AcuI CTGAAG 1 cut(s) 122
AfaI GTAC 1 cut(s) 203
AfiI CCNNNNNNNGG 1 cut(s) 128
AflIII ACRYGT 1 cut(s) 51
AgsI TTSAA 2 cut(s) 157, 183
AluBI AGCT 1 cut(s) 92
AluI AGCT 1 cut(s) 92
Alw26I GTCTC 3 cut(s) 23, 210, 216
Ama87I CYCGRG 1 cut(s) 213
AoxI GGCC 1 cut(s) 80
ApeKI GCWGC 1 cut(s) 170
ApoI RAATTY 1 cut(s) 38
ArsI GACNNNNNNTTYG 2 cut(s) 30, 62
AvaI CYCGRG 1 cut(s) 213
BbvI GCAGC 1 cut(s) 182
BcoDI GTCTC 3 cut(s) 23, 210, 216
BfaI CTAG 2 cut(s) 123, 241
BisI GCNGC 3 cut(s) 31, 34, 171
BlsI GCNGC 3 cut(s) 32, 35, 172
BmeT110I CYCGRG 1 cut(s) 213
BpmI CTGGAG 1 cut(s) 5
BsaBI GATNNNNATC 1 cut(s) 156
BsaI GGTCTC 1 cut(s) 23
BsaJI CCNNGG 1 cut(s) 31
Bsc4I CCNNNNNNNGG 1 cut(s) 128
Bse8I GATNNNNATC 1 cut(s) 156
BseDI CCNNGG 1 cut(s) 31
BseJI GATNNNNATC 1 cut(s) 156
BseLI CCNNNNNNNGG 1 cut(s) 128
BseMII CTCAG 2 cut(s) 63, 177
BseXI GCAGC 1 cut(s) 182
Bsh1236I CGCG 2 cut(s) 33, 53
BshFI GGCC 1 cut(s) 82
BsiHKCI CYCGRG 1 cut(s) 213
BslFI GGGAC 1 cut(s) 104
BslI CCNNNNNNNGG 1 cut(s) 128
BsmAI GTCTC 3 cut(s) 23, 210, 216
BsmFI GGGAC 1 cut(s) 104
BsnI GGCC 1 cut(s) 82
Bso31I GGTCTC 1 cut(s) 23
BsoBI CYCGRG 1 cut(s) 213
BspACI CCGC 4 cut(s) 28, 31, 33, 136
BspANI GGCC 1 cut(s) 82
BspCNI CTCAG 2 cut(s) 64, 178
BspFNI CGCG 2 cut(s) 33, 53
BspTNI GGTCTC 1 cut(s) 23
BssECI CCNNGG 1 cut(s) 31
Bst4CI ACNGT 1 cut(s) 221
BstDEI CTNAG 2 cut(s) 72, 186
BstDSI CCRYGG 1 cut(s) 31
BstFNI CGCG 2 cut(s) 33, 53
BstMAI GTCTC 3 cut(s) 23, 210, 216
BstUI CGCG 2 cut(s) 33, 53
BstV1I GCAGC 1 cut(s) 182
BsuRI GGCC 1 cut(s) 82
BtgI CCRYGG 1 cut(s) 31
BtsI GCAGTG 1 cut(s) 19
BtsIMutI CAGTG 1 cut(s) 19
Cfr42I CCGCGG 1 cut(s) 34
CseI GACGC 1 cut(s) 59
Csp6I GTAC 1 cut(s) 202
CviJI RGCY 4 cut(s) 71, 82, 92, 173
CviKI_1 RGCY 4 cut(s) 71, 82, 92, 173
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 2 cut(s) 72, 186
Eco147I AGGCCT 1 cut(s) 82
Eco31I GGTCTC 1 cut(s) 23
Eco57I CTGAAG 1 cut(s) 122
Eco88I CYCGRG 1 cut(s) 213
FaiI YATR 2 cut(s) 60, 168
FaqI GGGAC 1 cut(s) 104
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 3 cut(s) 31, 34, 171
Fsp4HI GCNGC 3 cut(s) 31, 34, 171
FspBI CTAG 2 cut(s) 123, 241
GluI GCNGC 3 cut(s) 31, 34, 171
GsuI CTGGAG 1 cut(s) 5
HaeIII GGCC 1 cut(s) 82
HgaI GACGC 1 cut(s) 59
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HinfI GANTC 2 cut(s) 157, 190
Hpy166II GTNNAC 2 cut(s) 49, 202
Hpy188I TCNGA 2 cut(s) 102, 131
Hpy188III TCNNGA 6 cut(s) 22, 161, 194, 213, 215, 228
Hpy8I GTNNAC 2 cut(s) 49, 202
Hpy99I CGWCG 1 cut(s) 53
HpyCH4III ACNGT 1 cut(s) 221
HpyCH4V TGCA 1 cut(s) 170
HpyF3I CTNAG 2 cut(s) 72, 186
KspI CCGCGG 1 cut(s) 34
LpnPI CCDG 6 cut(s) 35, 96, 130, 187, 207, 213
Lsp1109I GCAGC 1 cut(s) 182
MaeI CTAG 2 cut(s) 123, 241
MaeIII GTNAC 1 cut(s) 54
MluCI AATT 1 cut(s) 38
MluI ACGCGT 1 cut(s) 51
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 1 cut(s) 60
MseI TTAA 1 cut(s) 108
MspA1I CMGCKG 1 cut(s) 33
MvnI CGCG 2 cut(s) 33, 53
PaeR7I CTCGAG 1 cut(s) 213
PceI AGGCCT 1 cut(s) 82
PfeI GAWTC 2 cut(s) 157, 190
PkrI GCNGC 3 cut(s) 32, 35, 172
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
SacII CCGCGG 1 cut(s) 34
SalI GTCGAC 1 cut(s) 47
SaqAI TTAA 1 cut(s) 108
SatI GCNGC 3 cut(s) 31, 34, 171
SetI ASST 1 cut(s) 94
Sfr274I CTCGAG 1 cut(s) 213
Sfr303I CCGCGG 1 cut(s) 34
SgrBI CCGCGG 1 cut(s) 34
SlaI CTCGAG 1 cut(s) 213
SmlI CTYRAG 1 cut(s) 213
SmoI CTYRAG 1 cut(s) 213
Sse9I AATT 1 cut(s) 38
SseBI AGGCCT 1 cut(s) 82
SsiI CCGC 4 cut(s) 28, 31, 33, 136
SspMI CTAG 2 cut(s) 123, 241
StuI AGGCCT 1 cut(s) 82
TaaI ACNGT 1 cut(s) 221
TaqI TCGA 2 cut(s) 48, 214
TasI AATT 1 cut(s) 38
TauI GCSGC 2 cut(s) 33, 36
TfiI GAWTC 2 cut(s) 157, 190
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TscAI CASTG 1 cut(s) 19
TseI GCWGC 1 cut(s) 170
TspDTI ATGAA 1 cut(s) 102
TspGWI ACGGA 1 cut(s) 164
TspRI CASTG 1 cut(s) 19
XapI RAATTY 1 cut(s) 38
XhoI CTCGAG 1 cut(s) 213
XmiI GTMKAC 1 cut(s) 48
XspI CTAG 2 cut(s) 123, 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.