Rh4CG215100
HSP70 Family

Heat shock 70 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0033644
Physical Location & Seq
Reverse (-)
44550427 .. 44553311
2885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG215100.1

Sequence Viewer

Length: 1128 bp
ATGATTAGCTGCAGTGAAGCCAAGTTTGATTTTTGCATCGGATTCTCTTTCAGAGTTTCACCTGTCTCACTGGGACCTTCCAGTACAGGAAGCTCTAGAACTTTAGCCCCTTTGTATTTTCTCCCCATACTCTGCAATCTCACACTTCCAAACCCGAAGCGATCCTTCTCTTCTCTGCTCGCTCCTGTGATCGAAACTCACTTCAAGCCTTGCTGTATCGCTCAACTGTCAACTGAATGGCTGAGGCAGCATACACAGTGGCATCTGATAGTGAAAGCAGTCGGGAGGAGAAATCCTCATGCACCTTTCCTGAAACAACAATTGGAATTGATATTGGCACTTCACAATGCAGGGTTGCGATCTGGAATGGCTCCCAGCAATCAACTCTCTGCAGATCATGATATGTTATCCGGAGCTGCAATCTTCAACATGAAACGCTTAATTGGCCGAGTTGACACGGATCCAGTTGTCCACTCTAGCAAAAGCCTTCCATTTCTAGTACAAACATTGGACATTGGTGTCCGCCCATTTATTGCAGCCTTGGTTGGAAATTTCTGGAGATCCACCGCTCCTGAAGAAGTGCTGGCAATATTTTTGGTGGAACTTAGGGAAATGGCAGAAATTCAGTTAAAGCGGCCCATAAAAAATGTTGTTCTGACTCTGCCAGTTTCATTCAGCCGATTCCAGCTGACTCGGATTGAGAGAGCATGTGCCATGGCTGGCCTTCATATCCTGAAACTGATGCCAGAGCCAACTGCAGTGGCATTGTTATATACCTTATATGCAAAACAAAGTGGATTTCAGTCAACAGATAGGAATGTTCTAATCTTCAATATGGGTGCTGGATACTGTGATGTCGCCGTAAGTGTTACAGCTGAAGAGGCACCTCGAGGAATTTCCGAAATAATTGCCTTAACAGGAAGTGCCATTGGAGGAGAAGACTTGCTTCAGAATATGATGCGTCATCTGTTGCCAAATGCAGAGAATCTTTTGACAAGTCATGGAGTTGATGAAATCAAATCAAAGGGGTTACTTCGAGTTGCGACCCAGAATGCAATCCACAGTCTCTCCTCCCAAAATTCTGTTCAAATTGATGTTGACTTGGGAAATGGGATGAAAATCTGCAAG

Protein Analysis

376

Amino Acids

41.19

Weight (kDa)

8.91

Isoelectric Point (pI)

51.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 140 - 367 1.4e-28 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0033644)

Species Orthologous Gene IDs
rosa_samantha Rh4CG215100 Rh6AG321900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 518
AbsI CCTCGAGG 1 cut(s) 888
AccB1I GGYRCC 1 cut(s) 883
AccBSI CCGCTC 1 cut(s) 569
AccIII TCCGGA 1 cut(s) 410
AciI CCGC 3 cut(s) 523, 567, 634
AclWI GGATC 4 cut(s) 156, 455, 468, 555
AcoI YGGCCR 1 cut(s) 445
AcsI RAATTY 4 cut(s) 550, 621, 894, 1078
AcuI CTGAAG 3 cut(s) 594, 897, 932
AfaI GTAC 2 cut(s) 85, 501
AgsI TTSAA 4 cut(s) 205, 427, 832, 1088
AjuI GAANNNNNNNTTGG 4 cut(s) 305, 337, 1068, 1100
AluBI AGCT 5 cut(s) 9, 93, 416, 688, 875
AluI AGCT 5 cut(s) 9, 93, 416, 688, 875
Alw26I GTCTC 2 cut(s) 70, 1070
AlwI GGATC 4 cut(s) 156, 455, 468, 555
Ama87I CYCGRG 1 cut(s) 888
Aor13HI TCCGGA 1 cut(s) 410
AoxI GGCC 3 cut(s) 445, 635, 721
ApeKI GCWGC 4 cut(s) 9, 247, 416, 536
ApoI RAATTY 4 cut(s) 550, 621, 894, 1078
AspS9I GGNCC 2 cut(s) 74, 636
AsuHPI GGTGA 1 cut(s) 51
AvaI CYCGRG 1 cut(s) 888
AvaII GGWCC 1 cut(s) 74
BamHI GGATCC 1 cut(s) 460
BanI GGYRCC 1 cut(s) 883
BbsI GAAGAC 1 cut(s) 945
BbvCI CCTCAGC 1 cut(s) 242
BbvI GCAGC 3 cut(s) 259, 403, 548
BceAI ACGGC 1 cut(s) 845
BcgI CGANNNNNNTGC 2 cut(s) 890, 924
BciVI GTATCC 1 cut(s) 839
BcoDI GTCTC 2 cut(s) 70, 1070
BfaI CTAG 3 cut(s) 96, 477, 497
BfmI CTRYAG 3 cut(s) 10, 390, 756
BfuI GTATCC 1 cut(s) 839
BisI GCNGC 5 cut(s) 10, 248, 417, 537, 635
BlsI GCNGC 5 cut(s) 11, 249, 418, 538, 636
Bme18I GGWCC 1 cut(s) 74
BmeT110I CYCGRG 1 cut(s) 888
BmgT120I GGNCC 2 cut(s) 74, 636
BmiI GGNNCC 4 cut(s) 75, 372, 462, 885
BmrI ACTGGG 1 cut(s) 80
BmsI GCATC 4 cut(s) 45, 271, 732, 948
BmuI ACTGGG 1 cut(s) 80
BpiI GAAGAC 1 cut(s) 945
BplI GAGNNNNNCTC 2 cut(s) 280, 312
BpmI CTGGAG 1 cut(s) 577
Bpu10I CCTNAGC 1 cut(s) 242
BsaBI GATNNNNATC 1 cut(s) 1118
BsaJI CCNNGG 2 cut(s) 540, 714
BsaWI WCCGGW 1 cut(s) 410
BsaXI ACNNNNNCTCC 2 cut(s) 1052, 1082
Bse1I ACTGG 4 cut(s) 75, 81, 464, 665
Bse8I GATNNNNATC 1 cut(s) 1118
BseAI TCCGGA 1 cut(s) 410
BseDI CCNNGG 2 cut(s) 540, 714
BseGI GGATG 1 cut(s) 1119
BseJI GATNNNNATC 1 cut(s) 1118
BseMII CTCAG 1 cut(s) 233
BseNI ACTGG 4 cut(s) 75, 81, 464, 665
BseRI GAGGAG 3 cut(s) 301, 948, 1060
BseXI GCAGC 3 cut(s) 259, 403, 548
BseYI CCCAGC 1 cut(s) 374
BshFI GGCC 3 cut(s) 447, 637, 723
BshNI GGYRCC 1 cut(s) 883
BsiHKCI CYCGRG 1 cut(s) 888
BsiSI CCGG 1 cut(s) 411
BslFI GGGAC 1 cut(s) 87
BsmAI GTCTC 2 cut(s) 70, 1070
BsmFI GGGAC 1 cut(s) 87
BsmI GAATGC 1 cut(s) 1057
BsnI GGCC 3 cut(s) 447, 637, 723
BsoBI CYCGRG 1 cut(s) 888
Bsp13I TCCGGA 1 cut(s) 410
Bsp143I GATC 6 cut(s) 161, 189, 359, 394, 460, 560
Bsp19I CCATGG 1 cut(s) 714
BspACI CCGC 3 cut(s) 523, 567, 634
BspANI GGCC 3 cut(s) 447, 637, 723
BspCNI CTCAG 1 cut(s) 234
BspEI TCCGGA 1 cut(s) 410
BspHI TCATGA 1 cut(s) 397
BspLI GGNNCC 4 cut(s) 75, 372, 462, 885
BspMAI CTGCAG 3 cut(s) 14, 394, 760
BspPI GGATC 4 cut(s) 156, 455, 468, 555
BspT107I GGYRCC 1 cut(s) 883
BsrBI CCGCTC 1 cut(s) 569
BsrI ACTGG 4 cut(s) 75, 81, 464, 665
BssECI CCNNGG 2 cut(s) 540, 714
BssMI GATC 6 cut(s) 161, 189, 359, 394, 460, 560
BssT1I CCWWGG 2 cut(s) 540, 714
Bst4CI ACNGT 4 cut(s) 228, 258, 851, 1064
Bst6I CTCTTC 2 cut(s) 175, 873
BstC8I GCNNGC 3 cut(s) 180, 585, 721
BstDEI CTNAG 2 cut(s) 242, 605
BstDSI CCRYGG 1 cut(s) 714
BstF5I GGATG 1 cut(s) 1119
BstKTI GATC 6 cut(s) 164, 192, 362, 397, 463, 563
BstMAI GTCTC 2 cut(s) 70, 1070
BstMBI GATC 6 cut(s) 161, 189, 359, 394, 460, 560
BstMWI GCNNNNNNNGC 3 cut(s) 247, 444, 881
BstNSI RCATGY 1 cut(s) 711
BstSFI CTRYAG 3 cut(s) 10, 390, 756
BstV1I GCAGC 3 cut(s) 259, 403, 548
BstV2I GAAGAC 1 cut(s) 945
BstX2I RGATCY 2 cut(s) 460, 560
BstYI RGATCY 2 cut(s) 460, 560
BsuI GTATCC 1 cut(s) 839
BsuRI GGCC 3 cut(s) 447, 637, 723
BtgI CCRYGG 1 cut(s) 714
BtsCI GGATG 1 cut(s) 1119
BtsI GCAGTG 2 cut(s) 19, 765
BtsIMutI CAGTG 4 cut(s) 19, 68, 263, 765
Cac8I GCNNGC 3 cut(s) 180, 585, 721
CciI TCATGA 1 cut(s) 397
Cfr13I GGNCC 2 cut(s) 74, 636
CseI GACGC 1 cut(s) 950
Csp6I GTAC 2 cut(s) 84, 500
CspCI CAANNNNNGTGG 2 cut(s) 741, 776
CviAII CATG 6 cut(s) 299, 398, 430, 708, 715, 1001
CviQI GTAC 2 cut(s) 84, 500
DdeI CTNAG 2 cut(s) 242, 605
DpnI GATC 6 cut(s) 163, 191, 361, 396, 462, 562
DpnII GATC 6 cut(s) 161, 189, 359, 394, 460, 560
DrdI GACNNNNNNGTC 1 cut(s) 518
DseDI GACNNNNNNGTC 1 cut(s) 518
EaeI YGGCCR 1 cut(s) 445
Eam1104I CTCTTC 2 cut(s) 175, 873
EarI CTCTTC 2 cut(s) 175, 873
EciI GGCGGA 1 cut(s) 512
Eco130I CCWWGG 2 cut(s) 540, 714
Eco47I GGWCC 1 cut(s) 74
Eco57I CTGAAG 3 cut(s) 594, 897, 932
Eco88I CYCGRG 1 cut(s) 888
EcoO109I RGGNCCY 1 cut(s) 74
EcoT14I CCWWGG 2 cut(s) 540, 714
ErhI CCWWGG 2 cut(s) 540, 714
FaeI CATG 6 cut(s) 302, 401, 433, 711, 718, 1004
FalI AAGNNNNNCTT 4 cut(s) 149, 181, 930, 962
FaqI GGGAC 1 cut(s) 87
FatI CATG 6 cut(s) 298, 397, 429, 707, 714, 1000
Fnu4HI GCNGC 5 cut(s) 10, 248, 417, 537, 635
Fsp4HI GCNGC 5 cut(s) 10, 248, 417, 537, 635
FspBI CTAG 3 cut(s) 96, 477, 497
GluI GCNGC 5 cut(s) 10, 248, 417, 537, 635
GsaI CCCAGC 1 cut(s) 378
GsuI CTGGAG 1 cut(s) 577
HaeIII GGCC 3 cut(s) 447, 637, 723
HapII CCGG 1 cut(s) 411
HgaI GACGC 1 cut(s) 950
Hin1II CATG 6 cut(s) 302, 401, 433, 711, 718, 1004
HincII GTYRAC 4 cut(s) 231, 454, 807, 1099
HindII GTYRAC 4 cut(s) 231, 454, 807, 1099
HinfI GANTC 5 cut(s) 42, 658, 681, 691, 985
HpaII CCGG 1 cut(s) 411
HphI GGTGA 1 cut(s) 51
Hpy166II GTNNAC 5 cut(s) 231, 454, 472, 807, 1099
Hpy188I TCNGA 7 cut(s) 41, 53, 267, 657, 696, 901, 951
Hpy188III TCNNGA 9 cut(s) 96, 283, 310, 363, 398, 411, 556, 572, 733
Hpy8I GTNNAC 5 cut(s) 231, 454, 472, 807, 1099
HpyAV CCTTC 4 cut(s) 87, 175, 497, 734
HpyCH4III ACNGT 4 cut(s) 228, 258, 851, 1064
HpyF10VI GCNNNNNNNGC 3 cut(s) 247, 444, 881
HpyF3I CTNAG 2 cut(s) 242, 605
Hsp92II CATG 6 cut(s) 302, 401, 433, 711, 718, 1004
Kpn2I TCCGGA 1 cut(s) 410
Kzo9I GATC 6 cut(s) 161, 189, 359, 394, 460, 560
LmnI GCTCC 4 cut(s) 187, 376, 413, 574
Lsp1109I GCAGC 3 cut(s) 259, 403, 548
LweI GCATC 4 cut(s) 45, 271, 732, 948
MaeI CTAG 3 cut(s) 96, 477, 497
MaeIII GTNAC 2 cut(s) 868, 1029
MalI GATC 6 cut(s) 163, 191, 361, 396, 462, 562
MbiI CCGCTC 1 cut(s) 569
MboI GATC 6 cut(s) 161, 189, 359, 394, 460, 560
MboII GAAGA 6 cut(s) 162, 415, 587, 820, 890, 950
MfeI CAATTG 1 cut(s) 320
MflI RGATCY 2 cut(s) 460, 560
MluCI AATT 9 cut(s) 320, 326, 441, 550, 621, 894, 906, 1078, 1089
MlyI GAGTC 2 cut(s) 652, 685
MmeI TCCRAC 1 cut(s) 526
MnlI CCTC 8 cut(s) 237, 279, 306, 874, 884, 897, 926, 1081
MroI TCCGGA 1 cut(s) 410
MseI TTAA 3 cut(s) 440, 629, 914
MspA1I CMGCKG 2 cut(s) 688, 875
MspI CCGG 1 cut(s) 411
MunI CAATTG 1 cut(s) 320
Mva1269I GAATGC 1 cut(s) 1057
MwoI GCNNNNNNNGC 3 cut(s) 247, 444, 881
NcoI CCATGG 1 cut(s) 714
NdeII GATC 6 cut(s) 161, 189, 359, 394, 460, 560
NlaIII CATG 6 cut(s) 302, 401, 433, 711, 718, 1004
NlaIV GGNNCC 4 cut(s) 75, 372, 462, 885
NmeAIII GCCGAG 1 cut(s) 473
NspI RCATGY 1 cut(s) 711
PaeR7I CTCGAG 1 cut(s) 888
PagI TCATGA 1 cut(s) 397
PctI GAATGC 1 cut(s) 1057
PfeI GAWTC 3 cut(s) 42, 681, 985
PkrI GCNGC 5 cut(s) 11, 249, 418, 538, 636
PleI GAGTC 2 cut(s) 652, 685
PpsI GAGTC 2 cut(s) 652, 685
PpuMI RGGWCCY 1 cut(s) 74
Psp5II RGGWCCY 1 cut(s) 74
PspFI CCCAGC 1 cut(s) 374
PspN4I GGNNCC 4 cut(s) 75, 372, 462, 885
PspPI GGNCC 2 cut(s) 74, 636
PspPPI RGGWCCY 1 cut(s) 74
PspXI VCTCGAGB 1 cut(s) 888
PstI CTGCAG 3 cut(s) 14, 394, 760
PsuI RGATCY 2 cut(s) 460, 560
PvuII CAGCTG 2 cut(s) 688, 875
RsaI GTAC 2 cut(s) 85, 501
RsaNI GTAC 2 cut(s) 84, 500
SaqAI TTAA 3 cut(s) 440, 629, 914
SatI GCNGC 5 cut(s) 10, 248, 417, 537, 635
Sau3AI GATC 6 cut(s) 161, 189, 359, 394, 460, 560
Sau96I GGNCC 2 cut(s) 74, 636
SchI GAGTC 2 cut(s) 652, 685
SfaNI GCATC 4 cut(s) 45, 271, 732, 948
SfcI CTRYAG 3 cut(s) 10, 390, 756
Sfr274I CTCGAG 1 cut(s) 888
SinI GGWCC 1 cut(s) 74
SlaI CTCGAG 1 cut(s) 888
SmlI CTYRAG 1 cut(s) 888
SmoI CTYRAG 1 cut(s) 888
Sse9I AATT 9 cut(s) 320, 326, 441, 550, 621, 894, 906, 1078, 1089
SsiI CCGC 3 cut(s) 523, 567, 634
SspI AATATT 1 cut(s) 591
SspMI CTAG 3 cut(s) 96, 477, 497
StyI CCWWGG 2 cut(s) 540, 714
TaaI ACNGT 4 cut(s) 228, 258, 851, 1064
TaqI TCGA 3 cut(s) 192, 889, 1036
TasI AATT 9 cut(s) 320, 326, 441, 550, 621, 894, 906, 1078, 1089
TatI WGTACW 2 cut(s) 83, 499
TauI GCSGC 1 cut(s) 637
TfiI GAWTC 3 cut(s) 42, 681, 985
Tru1I TTAA 3 cut(s) 440, 629, 914
Tru9I TTAA 3 cut(s) 440, 629, 914
TscAI CASTG 4 cut(s) 19, 75, 263, 765
TseI GCWGC 4 cut(s) 9, 247, 416, 536
TspDTI ATGAA 4 cut(s) 446, 660, 716, 1026
TspGWI ACGGA 1 cut(s) 473
TspRI CASTG 4 cut(s) 19, 75, 263, 765
VpaK11BI GGWCC 1 cut(s) 74
XapI RAATTY 4 cut(s) 550, 621, 894, 1078
XbaI TCTAGA 1 cut(s) 95
XceI RCATGY 1 cut(s) 711
XhoI CTCGAG 1 cut(s) 888
XspI CTAG 3 cut(s) 96, 477, 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.