Rh6AG321900
HSP70 Family

Heat shock 70 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0033644
Physical Location & Seq
Forward (+)
52216899 .. 52218146
1248 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG321900.1

Sequence Viewer

Length: 1248 bp
ATGGCTGAGGCAGCATACACAGTGGCATCTGATAGTGAAACCAGTCTGGAGGAGAAATCCTCATGCACCTTTCCTGAAATAACAGTTGGAATTGATATTGGTACCTCACAATGCAGGGTTGCAATCTGGAATGGCTCCCAGGTTGAGATCCTTAAAAACAGTAGGAACCAGAAGATGATAAGTTCATATGTTACGTTCAAGGATGGAACTCCTTCTGGGGGACTAAGCAATCAACTCTCTGCAGATCATGATATATTATCCGGAGCTGCAATATTCAACATGAAACACTTAATTGGCCGAGTTGACACGGATCCAGTTGTCCACTCTAGCAAAAGCCTTCCATTTCTAGTACAAACATTGGACATTGGTGTCCGTCCATTTATTGCAGCCTTGGTTGGAAATTTCTGGAGATCCACCACTCCTGAAGAAGTTCTGGCAATATTTTTGGTGGAACTTAGGGAAATGGCAGAAATTCAGTTAAGGCGGCCCATAAAAAATGTTGTTATGACTGTGCCTGTTTCATTCAGCCGATTCCAGTTGACTCGGATTGAGAGAGCATGTGCCGTGGCTGGCCTTCATATCCTGAAACTGATGCCAGAACCAACTGCAGTGGCATTGTTATATACCTTATATACAAAACAAAGGGGATTTCAGTCAACAGATAGGAATGTTCTAATCTTCAATATGGGTGCAGGATACTGTGATGTTGCCATAAGTGTGACAGCTGAAGAGGCACCACGAGGAATTTCTGAAATAATTGCCTTAACAGGAAGTGCCATTGGAGGAGAAGACTTGCTTCAGAATATGATGCGTCATCTGTTGCCAAATGCAGAGAATCTTTTGACAAGTCATGGAGTTGACGAAATCAAATCAAAGGGGTTACTTCGAGTTGCAACACAGAATGCAATCCAGAGTCTCTCCTCCCAAAATTCTGTTCAAATTGATGTTGACTTGGGAAATGGGATGAAAATCTGCAAGGTAGTGGACCGAGAAGAATTTGAGGAAGTTAATCGTGCGGTATTTAATAAGTGTGTTAGCCTCATAATCCAGTGTTTGCATGATGCAAAGGTAAAACCTGAGGATGTGAACGATGTAGTACTTGTCGGTGGATGTTCATACATTCCAAAGATAAAAAAATCTTGTCATGAGTGTATGCAAGAAAGAGGAGCTTTACGAAGGGATGAACCCATTGGAAGCTGCTGTCTCTGGAGCAGCACTACAAGGATACGTAGCTTCAGGTGTTCATGA

Protein Analysis

415

Amino Acids

45.58

Weight (kDa)

6.28

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 29 - 386 1.5e-58 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0033644)

Species Orthologous Gene IDs
rosa_samantha Rh4CG215100 Rh6AG321900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 368
Acc65I GGTACC 1 cut(s) 101
AccB1I GGYRCC 2 cut(s) 101, 733
AccIII TCCGGA 1 cut(s) 260
AciI CCGC 2 cut(s) 484, 1016
AclWI GGATC 4 cut(s) 142, 305, 318, 405
AcoI YGGCCR 1 cut(s) 295
AcsI RAATTY 5 cut(s) 400, 471, 744, 928, 995
AcuI CTGAAG 4 cut(s) 444, 747, 782, 1219
AfaI GTAC 3 cut(s) 103, 351, 1098
AfiI CCNNNNNNNGG 1 cut(s) 218
AgsI TTSAA 4 cut(s) 199, 277, 682, 938
AjnI CCWGG 1 cut(s) 138
AjuI GAANNNNNNNTTGG 4 cut(s) 69, 101, 918, 950
AluBI AGCT 5 cut(s) 266, 725, 1169, 1197, 1233
AluI AGCT 5 cut(s) 266, 725, 1169, 1197, 1233
Alw26I GTCTC 2 cut(s) 920, 1208
AlwI GGATC 4 cut(s) 142, 305, 318, 405
Aor13HI TCCGGA 1 cut(s) 260
AoxI GGCC 3 cut(s) 295, 485, 571
ApeKI GCWGC 5 cut(s) 11, 266, 386, 1197, 1212
ApoI RAATTY 5 cut(s) 400, 471, 744, 928, 995
Asp700I GAANNNNTTC 2 cut(s) 211, 429
Asp718I GGTACC 1 cut(s) 101
AspS9I GGNCC 2 cut(s) 486, 985
AvaII GGWCC 1 cut(s) 985
AxyI CCTNAGG 1 cut(s) 1077
BamHI GGATCC 1 cut(s) 310
BanI GGYRCC 2 cut(s) 101, 733
BauI CACGAG 1 cut(s) 738
BbsI GAAGAC 1 cut(s) 795
BbvCI CCTCAGC 1 cut(s) 6
BbvI GCAGC 5 cut(s) 23, 253, 398, 1184, 1224
BccI CCATC 1 cut(s) 197
BceAI ACGGC 1 cut(s) 548
BciT130I CCWGG 1 cut(s) 140
BciVI GTATCC 2 cut(s) 689, 1218
BcoDI GTCTC 2 cut(s) 920, 1208
BfaI CTAG 2 cut(s) 327, 347
BfmI CTRYAG 2 cut(s) 240, 606
BfuI GTATCC 2 cut(s) 689, 1218
BisI GCNGC 6 cut(s) 12, 267, 387, 485, 1198, 1213
BlsI GCNGC 6 cut(s) 13, 268, 388, 486, 1199, 1214
BmcAI AGTACT 1 cut(s) 1098
Bme1390I CCNGG 1 cut(s) 140
Bme18I GGWCC 1 cut(s) 985
BmgT120I GGNCC 2 cut(s) 486, 985
BmiI GGNNCC 5 cut(s) 103, 136, 167, 312, 735
BmrFI CCNGG 1 cut(s) 140
BmsI GCATC 4 cut(s) 35, 582, 798, 1051
BpiI GAAGAC 1 cut(s) 795
BplI GAGNNNNNCTC 2 cut(s) 44, 76
BpmI CTGGAG 3 cut(s) 68, 427, 1228
Bpu10I CCTNAGC 1 cut(s) 6
BsaAI YACGTR 1 cut(s) 1229
BsaBI GATNNNNATC 1 cut(s) 968
BsaJI CCNNGG 3 cut(s) 138, 390, 564
BsaWI WCCGGW 1 cut(s) 260
Bsc4I CCNNNNNNNGG 1 cut(s) 218
Bse1I ACTGG 4 cut(s) 42, 314, 535, 1048
Bse21I CCTNAGG 1 cut(s) 1077
Bse8I GATNNNNATC 1 cut(s) 968
BseAI TCCGGA 1 cut(s) 260
BseBI CCWGG 1 cut(s) 140
BseDI CCNNGG 3 cut(s) 138, 390, 564
BseGI GGATG 5 cut(s) 208, 969, 1087, 1115, 1186
BseJI GATNNNNATC 1 cut(s) 968
BseLI CCNNNNNNNGG 1 cut(s) 218
BseMII CTCAG 1 cut(s) 1068
BseNI ACTGG 4 cut(s) 42, 314, 535, 1048
BseRI GAGGAG 4 cut(s) 65, 798, 910, 1179
BseXI GCAGC 5 cut(s) 23, 253, 398, 1184, 1224
BsgI GTGCAG 1 cut(s) 711
BshFI GGCC 3 cut(s) 297, 487, 573
BshNI GGYRCC 2 cut(s) 101, 733
BsiSI CCGG 1 cut(s) 261
BslFI GGGAC 1 cut(s) 234
BslI CCNNNNNNNGG 1 cut(s) 218
BsmAI GTCTC 2 cut(s) 920, 1208
BsmFI GGGAC 1 cut(s) 234
BsmI GAATGC 1 cut(s) 907
BsnI GGCC 3 cut(s) 297, 487, 573
Bsp13I TCCGGA 1 cut(s) 260
Bsp143I GATC 4 cut(s) 147, 244, 310, 410
BspACI CCGC 2 cut(s) 484, 1016
BspANI GGCC 3 cut(s) 297, 487, 573
BspCNI CTCAG 1 cut(s) 1069
BspEI TCCGGA 1 cut(s) 260
BspHI TCATGA 3 cut(s) 247, 1144, 1244
BspLI GGNNCC 5 cut(s) 103, 136, 167, 312, 735
BspMAI CTGCAG 2 cut(s) 244, 610
BspPI GGATC 4 cut(s) 142, 305, 318, 405
BspT107I GGYRCC 2 cut(s) 101, 733
BsrI ACTGG 4 cut(s) 42, 314, 535, 1048
BssECI CCNNGG 3 cut(s) 138, 390, 564
BssMI GATC 4 cut(s) 147, 244, 310, 410
BssSI CACGAG 1 cut(s) 738
BssT1I CCWWGG 1 cut(s) 390
Bst2BI CACGAG 1 cut(s) 738
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 5 cut(s) 22, 85, 161, 511, 701
Bst6I CTCTTC 1 cut(s) 723
BstBAI YACGTR 1 cut(s) 1229
BstC8I GCNNGC 1 cut(s) 571
BstDEI CTNAG 4 cut(s) 6, 224, 455, 1077
BstDSI CCRYGG 1 cut(s) 564
BstF5I GGATG 5 cut(s) 208, 969, 1087, 1115, 1186
BstKTI GATC 4 cut(s) 150, 247, 313, 413
BstMAI GTCTC 2 cut(s) 920, 1208
BstMBI GATC 4 cut(s) 147, 244, 310, 410
BstMWI GCNNNNNNNGC 2 cut(s) 11, 731
BstNI CCWGG 1 cut(s) 140
BstNSI RCATGY 1 cut(s) 561
BstSCI CCNGG 1 cut(s) 138
BstSFI CTRYAG 2 cut(s) 240, 606
BstSNI TACGTA 1 cut(s) 1229
BstV1I GCAGC 5 cut(s) 23, 253, 398, 1184, 1224
BstV2I GAAGAC 1 cut(s) 795
BstX2I RGATCY 3 cut(s) 147, 310, 410
BstYI RGATCY 3 cut(s) 147, 310, 410
Bsu36I CCTNAGG 1 cut(s) 1077
BsuI GTATCC 2 cut(s) 689, 1218
BsuRI GGCC 3 cut(s) 297, 487, 573
BtgI CCRYGG 1 cut(s) 564
BtsCI GGATG 5 cut(s) 208, 969, 1087, 1115, 1186
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 3 cut(s) 27, 615, 1055
Cac8I GCNNGC 1 cut(s) 571
CciI TCATGA 3 cut(s) 247, 1144, 1244
Cfr13I GGNCC 2 cut(s) 486, 985
CseI GACGC 1 cut(s) 800
Csp6I GTAC 3 cut(s) 102, 350, 1097
CspCI CAANNNNNGTGG 2 cut(s) 591, 626
CviAII CATG 8 cut(s) 63, 248, 280, 558, 851, 1058, 1145, 1245
CviQI GTAC 3 cut(s) 102, 350, 1097
DdeI CTNAG 4 cut(s) 6, 224, 455, 1077
DpnI GATC 4 cut(s) 149, 246, 312, 412
DpnII GATC 4 cut(s) 147, 244, 310, 410
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
EaeI YGGCCR 1 cut(s) 295
Eam1104I CTCTTC 1 cut(s) 723
EarI CTCTTC 1 cut(s) 723
Eco105I TACGTA 1 cut(s) 1229
Eco130I CCWWGG 1 cut(s) 390
Eco47I GGWCC 1 cut(s) 985
Eco57I CTGAAG 4 cut(s) 444, 747, 782, 1219
Eco81I CCTNAGG 1 cut(s) 1077
EcoRII CCWGG 1 cut(s) 138
EcoT14I CCWWGG 1 cut(s) 390
ErhI CCWWGG 1 cut(s) 390
FaeI CATG 8 cut(s) 66, 251, 283, 561, 854, 1061, 1148, 1248
FalI AAGNNNNNCTT 4 cut(s) 780, 812, 1153, 1185
FaqI GGGAC 1 cut(s) 234
FatI CATG 8 cut(s) 62, 247, 279, 557, 850, 1057, 1144, 1244
FauNDI CATATG 1 cut(s) 187
Fnu4HI GCNGC 6 cut(s) 12, 267, 387, 485, 1198, 1213
FokI GGATG 5 cut(s) 215, 976, 1094, 1122, 1193
Fsp4HI GCNGC 6 cut(s) 12, 267, 387, 485, 1198, 1213
FspBI CTAG 2 cut(s) 327, 347
GluI GCNGC 6 cut(s) 12, 267, 387, 485, 1198, 1213
GsuI CTGGAG 3 cut(s) 68, 427, 1228
HaeIII GGCC 3 cut(s) 297, 487, 573
HapII CCGG 1 cut(s) 261
HgaI GACGC 1 cut(s) 800
Hin1II CATG 8 cut(s) 66, 251, 283, 561, 854, 1061, 1148, 1248
HincII GTYRAC 5 cut(s) 304, 540, 657, 859, 949
HindII GTYRAC 5 cut(s) 304, 540, 657, 859, 949
HinfI GANTC 4 cut(s) 531, 541, 835, 913
HpaII CCGG 1 cut(s) 261
Hpy166II GTNNAC 8 cut(s) 304, 322, 540, 657, 859, 949, 985, 1087
Hpy188I TCNGA 4 cut(s) 31, 546, 751, 801
Hpy8I GTNNAC 8 cut(s) 304, 322, 540, 657, 859, 949, 985, 1087
HpyAV CCTTC 4 cut(s) 222, 347, 584, 1170
HpyCH4III ACNGT 5 cut(s) 22, 85, 161, 511, 701
HpyCH4IV ACGT 2 cut(s) 194, 1228
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 731
HpyF3I CTNAG 4 cut(s) 6, 224, 455, 1077
HpySE526I ACGT 2 cut(s) 194, 1228
Hsp92II CATG 8 cut(s) 66, 251, 283, 561, 854, 1061, 1148, 1248
Kpn2I TCCGGA 1 cut(s) 260
KpnI GGTACC 1 cut(s) 105
Kzo9I GATC 4 cut(s) 147, 244, 310, 410
LmnI GCTCC 4 cut(s) 140, 263, 1166, 1209
Lsp1109I GCAGC 5 cut(s) 23, 253, 398, 1184, 1224
LweI GCATC 4 cut(s) 35, 582, 798, 1051
MaeI CTAG 2 cut(s) 327, 347
MaeII ACGT 2 cut(s) 194, 1228
MaeIII GTNAC 3 cut(s) 190, 718, 879
MalI GATC 4 cut(s) 149, 246, 312, 412
MboI GATC 4 cut(s) 147, 244, 310, 410
MboII GAAGA 6 cut(s) 184, 437, 670, 740, 800, 1004
MflI RGATCY 3 cut(s) 147, 310, 410
MluCI AATT 9 cut(s) 90, 291, 400, 471, 744, 756, 928, 939, 995
MlyI GAGTC 2 cut(s) 535, 922
MmeI TCCRAC 2 cut(s) 67, 376
MroI TCCGGA 1 cut(s) 260
MroXI GAANNNNTTC 2 cut(s) 211, 429
MseI TTAA 6 cut(s) 153, 290, 479, 764, 1008, 1023
MslI CAYNNNNRTG 1 cut(s) 716
MspA1I CMGCKG 1 cut(s) 725
MspI CCGG 1 cut(s) 261
MspR9I CCNGG 1 cut(s) 140
Mva1269I GAATGC 1 cut(s) 907
MvaI CCWGG 1 cut(s) 140
MwoI GCNNNNNNNGC 2 cut(s) 11, 731
NdeI CATATG 1 cut(s) 187
NdeII GATC 4 cut(s) 147, 244, 310, 410
NlaIII CATG 8 cut(s) 66, 251, 283, 561, 854, 1061, 1148, 1248
NlaIV GGNNCC 5 cut(s) 103, 136, 167, 312, 735
NmeAIII GCCGAG 1 cut(s) 323
NmuCI GTSAC 1 cut(s) 718
NspI RCATGY 1 cut(s) 561
PagI TCATGA 3 cut(s) 247, 1144, 1244
PctI GAATGC 1 cut(s) 907
PdmI GAANNNNTTC 2 cut(s) 211, 429
PfeI GAWTC 2 cut(s) 531, 835
PkrI GCNGC 6 cut(s) 13, 268, 388, 486, 1199, 1214
PleI GAGTC 2 cut(s) 535, 921
PpsI GAGTC 2 cut(s) 535, 921
Ppu21I YACGTR 1 cut(s) 1229
Psp6I CCWGG 1 cut(s) 138
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 5 cut(s) 103, 136, 167, 312, 735
PspPI GGNCC 2 cut(s) 486, 985
PstI CTGCAG 2 cut(s) 244, 610
PsuI RGATCY 3 cut(s) 147, 310, 410
PvuII CAGCTG 1 cut(s) 725
RsaI GTAC 3 cut(s) 103, 351, 1098
RsaNI GTAC 3 cut(s) 102, 350, 1097
RseI CAYNNNNRTG 1 cut(s) 716
SaqAI TTAA 6 cut(s) 153, 290, 479, 764, 1008, 1023
SatI GCNGC 6 cut(s) 12, 267, 387, 485, 1198, 1213
Sau3AI GATC 4 cut(s) 147, 244, 310, 410
Sau96I GGNCC 2 cut(s) 486, 985
ScaI AGTACT 1 cut(s) 1098
SchI GAGTC 2 cut(s) 535, 922
ScrFI CCNGG 1 cut(s) 140
SfaNI GCATC 4 cut(s) 35, 582, 798, 1051
SfcI CTRYAG 2 cut(s) 240, 606
SinI GGWCC 1 cut(s) 985
SmiMI CAYNNNNRTG 1 cut(s) 716
SnaBI TACGTA 1 cut(s) 1229
Sse9I AATT 9 cut(s) 90, 291, 400, 471, 744, 756, 928, 939, 995
SsiI CCGC 2 cut(s) 484, 1016
SspI AATATT 2 cut(s) 273, 441
SspMI CTAG 2 cut(s) 327, 347
StyD4I CCNGG 1 cut(s) 138
StyI CCWWGG 1 cut(s) 390
TaaI ACNGT 5 cut(s) 22, 85, 161, 511, 701
TaiI ACGT 2 cut(s) 197, 1231
TaqI TCGA 1 cut(s) 886
TaqII GACCGA 1 cut(s) 1002
TasI AATT 9 cut(s) 90, 291, 400, 471, 744, 756, 928, 939, 995
TatI WGTACW 2 cut(s) 349, 1096
TauI GCSGC 1 cut(s) 487
TfiI GAWTC 2 cut(s) 531, 835
Tru1I TTAA 6 cut(s) 153, 290, 479, 764, 1008, 1023
Tru9I TTAA 6 cut(s) 153, 290, 479, 764, 1008, 1023
TscAI CASTG 3 cut(s) 27, 615, 1055
TseFI GTSAC 1 cut(s) 718
TseI GCWGC 5 cut(s) 11, 266, 386, 1197, 1212
Tsp45I GTSAC 1 cut(s) 718
TspDTI ATGAA 8 cut(s) 174, 296, 510, 566, 980, 1104, 1197, 1233
TspGWI ACGGA 2 cut(s) 323, 362
TspRI CASTG 3 cut(s) 27, 615, 1055
VpaK11BI GGWCC 1 cut(s) 985
XapI RAATTY 5 cut(s) 400, 471, 744, 928, 995
XceI RCATGY 1 cut(s) 561
XmnI GAANNNNTTC 2 cut(s) 211, 429
XspI CTAG 2 cut(s) 327, 347
ZrmI AGTACT 1 cut(s) 1098
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.