Rh4CG245600

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0022214
Physical Location & Seq
Reverse (-)
49399480 .. 49404452
4973 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG245600.1

Sequence Viewer

Length: 996 bp
ATGGGTGGGTGCTTTCCTTGCTTTGGATCATCCAACAAGGAGGGTAGTGGTGGTGCTGGTGTGAAGGAAGTTAGCAAGAAGGAATCAGTTAAAGAAGGCTCAGCTACTCAGTCTCACCATGTTACCAGAGTTGGCTCAGATAAATCAAAATCTCGGAATGGTTCTGATCCTAAAAAGGAACCACCTATTCCGAAAGATGTACCTACAGCACACATTGCTGCACAAACATTTACTTTCCGGGAGCTTGCAGCTGCAACAAAGAATTTTAAGCCCGAGTGTTTATTGGGTGAAGGTGGTTTTGGACGTGTCTACAAAGGTCGCTTGGAGAGCACAGGGCAGATTACTGAAGTAGATGGGAATGCTGATGCACGACCACTTTTTAAAGATCGTAGGAAGTTCCCAAAAATGGCTGATCCACTACTACAGGGCCGTTATCCAATGCGGGGGCTTTACCAAGCTCTTGCTGTTGCAGCCATGTGCTTGCAGGAGCAAGCGGCTACAAGGCCTTTAATAGGAGATGTAGTGACTGCTCTTACATACTTGGCTTCTCAAACCTATGATCCAAATGCAGCTAGTGGTCACAGTAACAGAGTTGGTCCATCTACTCCTAGGCACAAGGACGATCGAAGGAACATGGGAGATGGGCTGGATAGCCCAGATGAGCCTGGACGAGGTGGACGGCATGGTAGCCATTCATACAGAAACTCACCTGATTTCAGAAGGAAAGATCCCAATAGGGACTTGGGTGCTGGTGTAGAATTAGGAAGGATTGAAACCGGTAACGGCTCTGGGAGGAAATGGGGTCTAGATGGGTTAGAGCAACAAGATTCTCAGAGGGACAGCCCTGTAAGTGCTGGGAGAGCAAGGGAAACTCTGCGAAATCGTGATCTAGATAGAGAACGTGCGGTGGCTGAGGCGAAGGTTTGGGGCGAAAACTGGAGAGAAAAGAAAAGAGCAAATGTGATGGGTGGTAGCTTTGATGGTACAAATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000187 GO:0001932 GO:0001934 GO:0002218 GO:0002221 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0002757 GO:0002758 GO:0002764 GO:0002831 GO:0002833 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009814 GO:0009816 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019220 GO:0019222 GO:0019538 GO:0023051 GO:0023052 GO:0023056 GO:0031224 GO:0031225 GO:0031226 GO:0031323 GO:0031325 GO:0031347 GO:0031349 GO:0031399 GO:0031401 GO:0032101 GO:0032103 GO:0032147 GO:0032268 GO:0032270 GO:0033674 GO:0035690 GO:0036211 GO:0038023 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0043902 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044425 GO:0044459 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0045859 GO:0045860 GO:0045937 GO:0046658 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0098542 GO:0140096 GO:1900150 GO:1900424 GO:1900426 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

331

Amino Acids

35.95

Weight (kDa)

9.44

Isoelectric Point (pI)

40.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022214)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g17410
pyrus_communis pycom13g14800
rosa_samantha Rh4AG231200 Rh4CG245600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 309
AciI CCGC 3 cut(s) 442, 494, 905
AclWI GGATC 5 cut(s) 34, 161, 407, 554, 722
AcsI RAATTY 1 cut(s) 262
AcuI CTGAAG 1 cut(s) 366
AfaI GTAC 2 cut(s) 201, 985
AfiI CCNNNNNNNGG 5 cut(s) 23, 406, 443, 512, 671
AflIII ACRYGT 1 cut(s) 304
AgeI ACCGGT 1 cut(s) 776
AgsI TTSAA 1 cut(s) 773
AjiI CACGTC 1 cut(s) 305
AjnI CCWGG 1 cut(s) 664
AjuI GAANNNNNNNTTGG 2 cut(s) 282, 314
AluBI AGCT 6 cut(s) 104, 244, 251, 458, 572, 975
AluI AGCT 6 cut(s) 104, 244, 251, 458, 572, 975
Alw21I GWGCWC 1 cut(s) 332
Alw26I GTCTC 1 cut(s) 117
AlwI GGATC 5 cut(s) 34, 161, 407, 554, 722
Ama87I CYCGRG 1 cut(s) 272
AoxI GGCC 2 cut(s) 427, 503
ApeKI GCWGC 5 cut(s) 218, 248, 251, 470, 569
ApoI RAATTY 1 cut(s) 262
AsiGI ACCGGT 1 cut(s) 776
AspA2I CCTAGG 1 cut(s) 608
AspS9I GGNCC 2 cut(s) 427, 596
AsuC2I CCSGG 1 cut(s) 239
AsuHPI GGTGA 3 cut(s) 107, 299, 699
AvaI CYCGRG 1 cut(s) 272
AvaII GGWCC 1 cut(s) 596
AvrII CCTAGG 1 cut(s) 608
Bbv12I GWGCWC 1 cut(s) 332
BbvCI CCTCAGC 1 cut(s) 912
BbvI GCAGC 5 cut(s) 205, 238, 260, 482, 581
BccI CCATC 6 cut(s) 347, 607, 635, 803, 958, 974
BceAI ACGGC 3 cut(s) 414, 695, 799
BciT130I CCWGG 1 cut(s) 666
BcnI CCSGG 1 cut(s) 239
BcoDI GTCTC 1 cut(s) 117
BfaI CTAG 4 cut(s) 573, 609, 806, 890
BfmI CTRYAG 2 cut(s) 204, 422
BisI GCNGC 6 cut(s) 219, 249, 252, 471, 495, 570
BlnI CCTAGG 1 cut(s) 608
BlpI GCTNAGC 1 cut(s) 100
BlsI GCNGC 6 cut(s) 220, 250, 253, 472, 496, 571
Bme1390I CCNGG 2 cut(s) 239, 666
Bme18I GGWCC 1 cut(s) 596
BmeT110I CYCGRG 1 cut(s) 272
BmgBI CACGTC 1 cut(s) 305
BmgT120I GGNCC 2 cut(s) 427, 596
BmiI GGNNCC 1 cut(s) 180
BmrFI CCNGG 2 cut(s) 239, 666
BmsI GCATC 1 cut(s) 355
BpmI CTGGAG 1 cut(s) 958
Bpu10I CCTNAGC 1 cut(s) 912
Bpu1102I GCTNAGC 1 cut(s) 100
BpuMI CCSGG 1 cut(s) 239
BsaJI CCNNGG 1 cut(s) 608
BsaWI WCCGGW 1 cut(s) 776
BsaXI ACNNNNNCTCC 2 cut(s) 507, 537
Bsc4I CCNNNNNNNGG 5 cut(s) 23, 406, 443, 512, 671
Bse118I RCCGGY 1 cut(s) 776
Bse1I ACTGG 1 cut(s) 941
Bse3DI GCAATG 1 cut(s) 213
BseBI CCWGG 1 cut(s) 666
BseDI CCNNGG 1 cut(s) 608
BseGI GGATG 1 cut(s) 29
BseLI CCNNNNNNNGG 5 cut(s) 23, 406, 443, 512, 671
BseMI GCAATG 1 cut(s) 213
BseMII CTCAG 5 cut(s) 114, 122, 150, 845, 903
BseNI ACTGG 1 cut(s) 941
BseXI GCAGC 5 cut(s) 205, 238, 260, 482, 581
BseYI CCCAGC 1 cut(s) 854
BsgI GTGCAG 1 cut(s) 204
Bsh1285I CGRYCG 1 cut(s) 625
BshFI GGCC 2 cut(s) 429, 505
BshTI ACCGGT 1 cut(s) 776
BsiEI CGRYCG 1 cut(s) 625
BsiHKAI GWGCWC 1 cut(s) 332
BsiHKCI CYCGRG 1 cut(s) 272
BsiSI CCGG 2 cut(s) 238, 777
BslFI GGGAC 2 cut(s) 752, 851
BslI CCNNNNNNNGG 5 cut(s) 23, 406, 443, 512, 671
BsmAI GTCTC 1 cut(s) 117
BsmFI GGGAC 2 cut(s) 752, 851
BsmI GAATGC 1 cut(s) 364
BsnI GGCC 2 cut(s) 429, 505
BsoBI CYCGRG 1 cut(s) 272
Bsp1286I GDGCHC 1 cut(s) 332
Bsp143I GATC 8 cut(s) 26, 166, 385, 412, 559, 622, 727, 886
Bsp1720I GCTNAGC 1 cut(s) 100
BspACI CCGC 3 cut(s) 442, 494, 905
BspANI GGCC 2 cut(s) 429, 505
BspCNI CTCAG 5 cut(s) 113, 121, 149, 844, 904
BspLI GGNNCC 1 cut(s) 180
BspPI GGATC 5 cut(s) 34, 161, 407, 554, 722
BsrDI GCAATG 1 cut(s) 213
BsrFI RCCGGY 1 cut(s) 776
BsrI ACTGG 1 cut(s) 941
BssAI RCCGGY 1 cut(s) 776
BssECI CCNNGG 1 cut(s) 608
BssMI GATC 8 cut(s) 26, 166, 385, 412, 559, 622, 727, 886
BssT1I CCWWGG 1 cut(s) 608
Bst2UI CCWGG 1 cut(s) 666
Bst4CI ACNGT 1 cut(s) 584
BstAPI GCANNNNNTGC 1 cut(s) 215
BstC8I GCNNGC 3 cut(s) 246, 482, 492
BstDEI CTNAG 5 cut(s) 100, 108, 136, 831, 912
BstENI CCTNNNNNAGG 2 cut(s) 510, 669
BstF5I GGATG 1 cut(s) 29
BstKTI GATC 8 cut(s) 29, 169, 388, 415, 562, 625, 730, 889
BstMAI GTCTC 1 cut(s) 117
BstMBI GATC 8 cut(s) 26, 166, 385, 412, 559, 622, 727, 886
BstMCI CGRYCG 1 cut(s) 625
BstMWI GCNNNNNNNGC 5 cut(s) 18, 215, 327, 470, 860
BstNI CCWGG 1 cut(s) 666
BstSCI CCNGG 2 cut(s) 237, 664
BstSFI CTRYAG 2 cut(s) 204, 422
BstV1I GCAGC 5 cut(s) 205, 238, 260, 482, 581
BstX2I RGATCY 1 cut(s) 727
BstYI RGATCY 1 cut(s) 727
BsuRI GGCC 2 cut(s) 429, 505
BtrI CACGTC 1 cut(s) 305
BtsCI GGATG 1 cut(s) 29
Cac8I GCNNGC 3 cut(s) 246, 482, 492
Cfr10I RCCGGY 1 cut(s) 776
Cfr13I GGNCC 2 cut(s) 427, 596
Csp6I GTAC 2 cut(s) 200, 984
CspAI ACCGGT 1 cut(s) 776
CviAII CATG 4 cut(s) 119, 475, 634, 683
CviQI GTAC 2 cut(s) 200, 984
DdeI CTNAG 5 cut(s) 100, 108, 136, 831, 912
DpnI GATC 8 cut(s) 28, 168, 387, 414, 561, 624, 729, 888
DpnII GATC 8 cut(s) 26, 166, 385, 412, 559, 622, 727, 886
DraI TTTAAA 1 cut(s) 382
Eco130I CCWWGG 1 cut(s) 608
Eco147I AGGCCT 1 cut(s) 505
Eco47I GGWCC 1 cut(s) 596
Eco57I CTGAAG 1 cut(s) 366
Eco88I CYCGRG 1 cut(s) 272
EcoNI CCTNNNNNAGG 2 cut(s) 510, 669
EcoRII CCWGG 1 cut(s) 664
EcoT14I CCWWGG 1 cut(s) 608
ErhI CCWWGG 1 cut(s) 608
FaeI CATG 4 cut(s) 122, 478, 637, 686
FaiI YATR 7 cut(s) 120, 476, 538, 558, 635, 684, 697
FaqI GGGAC 2 cut(s) 752, 851
FatI CATG 4 cut(s) 118, 474, 633, 682
FauI CCCGC 1 cut(s) 435
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 6 cut(s) 219, 249, 252, 471, 495, 570
FokI GGATG 1 cut(s) 16
Fsp4HI GCNGC 6 cut(s) 219, 249, 252, 471, 495, 570
FspBI CTAG 4 cut(s) 573, 609, 806, 890
GluI GCNGC 6 cut(s) 219, 249, 252, 471, 495, 570
GsaI CCCAGC 1 cut(s) 858
GsuI CTGGAG 1 cut(s) 958
HaeIII GGCC 2 cut(s) 429, 505
HapII CCGG 2 cut(s) 238, 777
Hin1II CATG 4 cut(s) 122, 478, 637, 686
HinfI GANTC 2 cut(s) 83, 827
HpaII CCGG 2 cut(s) 238, 777
HphI GGTGA 3 cut(s) 107, 299, 699
Hpy166II GTNNAC 2 cut(s) 310, 677
Hpy188I TCNGA 6 cut(s) 139, 156, 166, 192, 719, 834
Hpy188III TCNNGA 3 cut(s) 806, 884, 890
Hpy8I GTNNAC 2 cut(s) 310, 677
HpyAV CCTTC 8 cut(s) 58, 73, 89, 284, 621, 714, 759, 913
HpyCH4III ACNGT 1 cut(s) 584
HpyCH4IV ACGT 2 cut(s) 304, 901
HpyCH4V TGCA 7 cut(s) 221, 248, 254, 368, 470, 484, 569
HpyF10VI GCNNNNNNNGC 5 cut(s) 18, 215, 327, 470, 860
HpyF3I CTNAG 5 cut(s) 100, 108, 136, 831, 912
HpySE526I ACGT 2 cut(s) 304, 901
Hsp92II CATG 4 cut(s) 122, 478, 637, 686
Kzo9I GATC 8 cut(s) 26, 166, 385, 412, 559, 622, 727, 886
LmnI GCTCC 2 cut(s) 241, 487
Lsp1109I GCAGC 5 cut(s) 205, 238, 260, 482, 581
LweI GCATC 1 cut(s) 355
MaeI CTAG 4 cut(s) 573, 609, 806, 890
MaeII ACGT 2 cut(s) 304, 901
MaeIII GTNAC 5 cut(s) 121, 523, 578, 584, 779
MalI GATC 8 cut(s) 28, 168, 387, 414, 561, 624, 729, 888
MboI GATC 8 cut(s) 26, 166, 385, 412, 559, 622, 727, 886
MflI RGATCY 1 cut(s) 727
MhlI GDGCHC 1 cut(s) 332
MluCI AATT 2 cut(s) 262, 758
MmeI TCCRAC 1 cut(s) 57
MnlI CCTC 5 cut(s) 34, 665, 786, 828, 907
MseI TTAA 4 cut(s) 90, 267, 381, 509
MspA1I CMGCKG 1 cut(s) 251
MspI CCGG 2 cut(s) 238, 777
MspR9I CCNGG 2 cut(s) 239, 666
Mva1269I GAATGC 1 cut(s) 364
MvaI CCWGG 1 cut(s) 666
MwoI GCNNNNNNNGC 5 cut(s) 18, 215, 327, 470, 860
NciI CCSGG 1 cut(s) 239
NdeII GATC 8 cut(s) 26, 166, 385, 412, 559, 622, 727, 886
NlaIII CATG 4 cut(s) 122, 478, 637, 686
NlaIV GGNNCC 1 cut(s) 180
NmuCI GTSAC 2 cut(s) 523, 578
PceI AGGCCT 1 cut(s) 505
PctI GAATGC 1 cut(s) 364
PfeI GAWTC 2 cut(s) 83, 827
PfoI TCCNGGA 1 cut(s) 237
PinAI ACCGGT 1 cut(s) 776
PkrI GCNGC 6 cut(s) 220, 250, 253, 472, 496, 571
Ple19I CGATCG 1 cut(s) 625
Psp6I CCWGG 1 cut(s) 664
PspFI CCCAGC 1 cut(s) 854
PspGI CCWGG 1 cut(s) 664
PspN4I GGNNCC 1 cut(s) 180
PspPI GGNCC 2 cut(s) 427, 596
PsuI RGATCY 1 cut(s) 727
PvuI CGATCG 1 cut(s) 625
PvuII CAGCTG 1 cut(s) 251
RsaI GTAC 2 cut(s) 201, 985
RsaNI GTAC 2 cut(s) 200, 984
SaqAI TTAA 4 cut(s) 90, 267, 381, 509
SatI GCNGC 6 cut(s) 219, 249, 252, 471, 495, 570
Sau3AI GATC 8 cut(s) 26, 166, 385, 412, 559, 622, 727, 886
Sau96I GGNCC 2 cut(s) 427, 596
ScrFI CCNGG 2 cut(s) 239, 666
SduI GDGCHC 1 cut(s) 332
SfaNI GCATC 1 cut(s) 355
SfcI CTRYAG 2 cut(s) 204, 422
SinI GGWCC 1 cut(s) 596
Sse9I AATT 2 cut(s) 262, 758
SseBI AGGCCT 1 cut(s) 505
SsiI CCGC 3 cut(s) 442, 494, 905
SspMI CTAG 4 cut(s) 573, 609, 806, 890
StuI AGGCCT 1 cut(s) 505
StyD4I CCNGG 2 cut(s) 237, 664
StyI CCWWGG 1 cut(s) 608
TaaI ACNGT 1 cut(s) 584
TaiI ACGT 2 cut(s) 307, 904
TaqI TCGA 1 cut(s) 625
TasI AATT 2 cut(s) 262, 758
TauI GCSGC 1 cut(s) 497
TfiI GAWTC 2 cut(s) 83, 827
Tru1I TTAA 4 cut(s) 90, 267, 381, 509
Tru9I TTAA 4 cut(s) 90, 267, 381, 509
TseFI GTSAC 2 cut(s) 523, 578
TseI GCWGC 5 cut(s) 218, 248, 251, 470, 569
Tsp45I GTSAC 2 cut(s) 523, 578
TspDTI ATGAA 1 cut(s) 684
VpaK11BI GGWCC 1 cut(s) 596
XagI CCTNNNNNAGG 2 cut(s) 510, 669
XapI RAATTY 1 cut(s) 262
XbaI TCTAGA 2 cut(s) 805, 889
XcmI CCANNNNNNNNNTGG 1 cut(s) 739
XmaJI CCTAGG 1 cut(s) 608
XmiI GTMKAC 1 cut(s) 309
XspI CTAG 4 cut(s) 573, 609, 806, 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.