Rh4CG402300

Involved in the retrieval of endoplasmic reticulum membrane proteins from the early Golgi compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0021865
Physical Location & Seq
Forward (+)
65058545 .. 65060707
2163 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG402300.1

Sequence Viewer

Length: 654 bp
ATGGCTCTGACATTTTGCGCCTCCACTGTTATTTCCAAGCTCAGCTTCCTCATGCAGGAAAGCCGTCACAAGTCTTGCGTCGCAATCCAGCTTACTCAGATGTTTCCCCTTGATAGCAATACGCCAATATTAGATAGAAGAAGAGGGACCAACCGACCGACACACCTCGAGCAACGAACCCCAAGAGGGCCCCACAATGGACACCGGAGCGGCGGGAGTGGGTCTCGCCGCGTCGTCGTCGTCGGCGGAGGACCCGTCGTCTCGGGGGATCGCCGCTCGACGTCGTCCAGGCGTGGAGCTTCGGCGTGGAGCTTCGGCGTGTCGCGCCGATACCAGCACCTGCTCGACAAAACGACGCCGCACGTCCTCCACCGCTGGCTCGCAACCCTCGGCGTGGCCTTGGTCTACGTCCTCCGCGTCTACTTCGTCCAGGGATTCTACATCATGTCATACGGCTTGGGGATCTACCTCCTCAACTTGCTCATCGGCTTTCTCTCTCCTCAGGTCGACCCGGAGATCCACGACCTTTCCGACGGCCCCAGCCTCCCGACCCGCGGATCCGACGAGTTCCGCCCCTTCGTTCGACGCCTCCCCGAGTTTAAATTCTGGTACTCAATTACAAAGGCGTTTTGCATTGCTTTCTTGATGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006890 GO:0008150 GO:0016192 GO:0048193 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rer1

Protein Analysis

217

Amino Acids

24.44

Weight (kDa)

10.55

Isoelectric Point (pI)

58.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rer1 PF03248 107 - 216 6.6e-46 Rer1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021865)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0343311 RchiOBHm_Chr7g0237871
rosa_samantha Rh4BG387700 Rh4CG402300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 348
AatII GACGTC 1 cut(s) 284
Acc36I ACCTGC 1 cut(s) 348
AccBSI CCGCTC 2 cut(s) 210, 276
AccI GTMKAC 3 cut(s) 405, 420, 507
AccII CGCG 4 cut(s) 231, 325, 417, 555
AclWI GGATC 5 cut(s) 276, 470, 511, 552, 565
AcsI RAATTY 1 cut(s) 602
AcyI GRCGYC 3 cut(s) 281, 356, 586
AfaI GTAC 1 cut(s) 611
AfiI CCNNNNNNNGG 5 cut(s) 55, 186, 197, 394, 554
AhdI GACNNNNNGTC 1 cut(s) 257
AjiI CACGTC 1 cut(s) 364
AjnI CCWGG 2 cut(s) 287, 429
AjuI GAANNNNNNNTTGG 2 cut(s) 29, 61
AluBI AGCT 5 cut(s) 40, 45, 91, 299, 312
AluI AGCT 5 cut(s) 40, 45, 91, 299, 312
Alw26I GTCTC 2 cut(s) 228, 265
AlwI GGATC 5 cut(s) 276, 470, 511, 552, 565
AlwNI CAGNNNCTG 1 cut(s) 340
Ama87I CYCGRG 3 cut(s) 167, 262, 593
AoxI GGCC 3 cut(s) 188, 396, 535
ApaI GGGCCC 1 cut(s) 192
ApoI RAATTY 1 cut(s) 602
AspLEI GCGC 2 cut(s) 20, 327
AspS9I GGNCC 5 cut(s) 147, 188, 189, 251, 536
AsuC2I CCSGG 1 cut(s) 512
AvaI CYCGRG 3 cut(s) 167, 262, 593
AvaII GGWCC 2 cut(s) 147, 251
AxyI CCTNAGG 1 cut(s) 501
BaeGI GKGCMC 1 cut(s) 192
BamHI GGATCC 1 cut(s) 557
BanII GRGCYC 1 cut(s) 192
BceAI ACGGC 3 cut(s) 48, 469, 550
BciT130I CCWGG 2 cut(s) 289, 431
BcnI CCSGG 1 cut(s) 512
BcoDI GTCTC 2 cut(s) 228, 265
BfuAI ACCTGC 1 cut(s) 348
BisI GCNGC 4 cut(s) 211, 229, 274, 359
BlpI GCTNAGC 1 cut(s) 41
BlsI GCNGC 4 cut(s) 212, 230, 275, 360
Bme1390I CCNGG 3 cut(s) 289, 431, 512
Bme18I GGWCC 2 cut(s) 147, 251
BmeRI GACNNNNNGTC 1 cut(s) 257
BmeT110I CYCGRG 3 cut(s) 167, 262, 593
BmgBI CACGTC 1 cut(s) 364
BmgT120I GGNCC 5 cut(s) 147, 188, 189, 251, 536
BmiI GGNNCC 6 cut(s) 148, 190, 191, 253, 538, 559
BmrFI CCNGG 3 cut(s) 289, 431, 512
BplI GAGNNNNNCTC 2 cut(s) 208, 240
Bpu1102I GCTNAGC 1 cut(s) 41
BpuMI CCSGG 1 cut(s) 512
BsaHI GRCGYC 3 cut(s) 281, 356, 586
BsaI GGTCTC 1 cut(s) 228
BsaJI CCNNGG 4 cut(s) 388, 399, 430, 553
BsaWI WCCGGW 1 cut(s) 204
BsaXI ACNNNNNCTCC 2 cut(s) 240, 270
Bsc4I CCNNNNNNNGG 5 cut(s) 55, 186, 197, 394, 554
Bse21I CCTNAGG 1 cut(s) 501
Bse3DI GCAATG 1 cut(s) 633
BseBI CCWGG 2 cut(s) 289, 431
BseDI CCNNGG 4 cut(s) 388, 399, 430, 553
BseLI CCNNNNNNNGG 5 cut(s) 55, 186, 197, 394, 554
BseMI GCAATG 1 cut(s) 633
BseMII CTCAG 3 cut(s) 55, 110, 515
BseRI GAGGAG 2 cut(s) 461, 489
BseSI GKGCMC 1 cut(s) 192
BseYI CCCAGC 1 cut(s) 539
Bsh1236I CGCG 4 cut(s) 231, 325, 417, 555
Bsh1285I CGRYCG 1 cut(s) 158
BshFI GGCC 3 cut(s) 190, 398, 537
BsiEI CGRYCG 1 cut(s) 158
BsiHKCI CYCGRG 3 cut(s) 167, 262, 593
BsiSI CCGG 2 cut(s) 205, 512
BslFI GGGAC 1 cut(s) 160
BslI CCNNNNNNNGG 5 cut(s) 55, 186, 197, 394, 554
BsmAI GTCTC 2 cut(s) 228, 265
BsmBI CGTCTC 1 cut(s) 265
BsmFI GGGAC 1 cut(s) 160
BsnI GGCC 3 cut(s) 190, 398, 537
Bso31I GGTCTC 1 cut(s) 228
BsoBI CYCGRG 3 cut(s) 167, 262, 593
Bsp120I GGGCCC 1 cut(s) 188
Bsp1286I GDGCHC 1 cut(s) 192
Bsp143I GATC 4 cut(s) 268, 462, 516, 557
Bsp1720I GCTNAGC 1 cut(s) 41
BspANI GGCC 3 cut(s) 190, 398, 537
BspCNI CTCAG 3 cut(s) 54, 109, 514
BspFNI CGCG 4 cut(s) 231, 325, 417, 555
BspLI GGNNCC 6 cut(s) 148, 190, 191, 253, 538, 559
BspMI ACCTGC 1 cut(s) 348
BspPI GGATC 5 cut(s) 276, 470, 511, 552, 565
BspTNI GGTCTC 1 cut(s) 228
BsrBI CCGCTC 2 cut(s) 210, 276
BsrDI GCAATG 1 cut(s) 633
BssECI CCNNGG 4 cut(s) 388, 399, 430, 553
BssMI GATC 4 cut(s) 268, 462, 516, 557
BssNI GRCGYC 3 cut(s) 281, 356, 586
BssT1I CCWWGG 1 cut(s) 399
Bst2UI CCWGG 2 cut(s) 289, 431
Bst4CI ACNGT 1 cut(s) 28
Bst6I CTCTTC 1 cut(s) 136
BstACI GRCGYC 3 cut(s) 281, 356, 586
BstC8I GCNNGC 2 cut(s) 377, 381
BstDEI CTNAG 3 cut(s) 41, 96, 501
BstDSI CCRYGG 1 cut(s) 553
BstENI CCTNNNNNAGG 1 cut(s) 53
BstFNI CGCG 4 cut(s) 231, 325, 417, 555
BstHHI GCGC 2 cut(s) 20, 327
BstKTI GATC 4 cut(s) 271, 465, 519, 560
BstMAI GTCTC 2 cut(s) 228, 265
BstMBI GATC 4 cut(s) 268, 462, 516, 557
BstMCI CGRYCG 1 cut(s) 158
BstMWI GCNNNNNNNGC 1 cut(s) 324
BstNI CCWGG 2 cut(s) 289, 431
BstSCI CCNGG 3 cut(s) 287, 429, 510
BstSLI GKGCMC 1 cut(s) 192
BstUI CGCG 4 cut(s) 231, 325, 417, 555
BstX2I RGATCY 3 cut(s) 462, 516, 557
BstYI RGATCY 3 cut(s) 462, 516, 557
Bsu36I CCTNAGG 1 cut(s) 501
BsuRI GGCC 3 cut(s) 190, 398, 537
BtgI CCRYGG 1 cut(s) 553
BtrI CACGTC 1 cut(s) 364
BtsIMutI CAGTG 1 cut(s) 24
BveI ACCTGC 1 cut(s) 348
Cac8I GCNNGC 2 cut(s) 377, 381
CaiI CAGNNNCTG 1 cut(s) 340
CfoI GCGC 2 cut(s) 20, 327
Cfr13I GGNCC 5 cut(s) 147, 188, 189, 251, 536
Cfr42I CCGCGG 1 cut(s) 556
CseI GACGC 5 cut(s) 67, 220, 364, 406, 594
Csp6I GTAC 1 cut(s) 610
CviAII CATG 2 cut(s) 52, 445
CviQI GTAC 1 cut(s) 610
DdeI CTNAG 3 cut(s) 41, 96, 501
DpnI GATC 4 cut(s) 270, 464, 518, 559
DpnII GATC 4 cut(s) 268, 462, 516, 557
DraI TTTAAA 1 cut(s) 601
DriI GACNNNNNGTC 1 cut(s) 257
Eam1104I CTCTTC 1 cut(s) 136
Eam1105I GACNNNNNGTC 1 cut(s) 257
EarI CTCTTC 1 cut(s) 136
EciI GGCGGA 2 cut(s) 261, 560
Eco130I CCWWGG 1 cut(s) 399
Eco24I GRGCYC 1 cut(s) 192
Eco31I GGTCTC 1 cut(s) 228
Eco47I GGWCC 2 cut(s) 147, 251
Eco81I CCTNAGG 1 cut(s) 501
Eco88I CYCGRG 3 cut(s) 167, 262, 593
EcoNI CCTNNNNNAGG 1 cut(s) 53
EcoO109I RGGNCCY 3 cut(s) 188, 189, 251
EcoRII CCWGG 2 cut(s) 287, 429
EcoT14I CCWWGG 1 cut(s) 399
EcoT38I GRGCYC 1 cut(s) 192
ErhI CCWWGG 1 cut(s) 399
Esp3I CGTCTC 1 cut(s) 265
FaeI CATG 2 cut(s) 55, 448
FaiI YATR 3 cut(s) 53, 446, 451
FalI AAGNNNNNCTT 2 cut(s) 29, 61
FaqI GGGAC 1 cut(s) 160
FatI CATG 2 cut(s) 51, 444
FauI CCCGC 2 cut(s) 206, 560
FblI GTMKAC 3 cut(s) 405, 420, 507
Fnu4HI GCNGC 4 cut(s) 211, 229, 274, 359
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 4 cut(s) 211, 229, 274, 359
GlaI GCGC 2 cut(s) 19, 326
GluI GCNGC 4 cut(s) 211, 229, 274, 359
GsaI CCCAGC 1 cut(s) 543
HaeIII GGCC 3 cut(s) 190, 398, 537
HapII CCGG 2 cut(s) 205, 512
HgaI GACGC 5 cut(s) 67, 220, 364, 406, 594
HhaI GCGC 2 cut(s) 20, 327
Hin1I GRCGYC 3 cut(s) 281, 356, 586
Hin1II CATG 2 cut(s) 55, 448
Hin6I GCGC 2 cut(s) 18, 325
HinP1I GCGC 2 cut(s) 18, 325
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HinfI GANTC 1 cut(s) 435
HpaII CCGG 2 cut(s) 205, 512
Hpy166II GTNNAC 3 cut(s) 406, 421, 508
Hpy188I TCNGA 4 cut(s) 9, 99, 532, 562
Hpy188III TCNNGA 2 cut(s) 547, 643
Hpy8I GTNNAC 3 cut(s) 406, 421, 508
HpyAV CCTTC 1 cut(s) 586
HpyCH4III ACNGT 1 cut(s) 28
HpyCH4IV ACGT 3 cut(s) 281, 363, 408
HpyCH4V TGCA 2 cut(s) 55, 633
HpyF10VI GCNNNNNNNGC 1 cut(s) 324
HpyF3I CTNAG 3 cut(s) 41, 96, 501
HpySE526I ACGT 3 cut(s) 281, 363, 408
Hsp92I GRCGYC 3 cut(s) 281, 356, 586
Hsp92II CATG 2 cut(s) 55, 448
HspAI GCGC 2 cut(s) 18, 325
KspI CCGCGG 1 cut(s) 556
Kzo9I GATC 4 cut(s) 268, 462, 516, 557
LmnI GCTCC 3 cut(s) 207, 296, 309
MaeII ACGT 3 cut(s) 281, 363, 408
MaeIII GTNAC 1 cut(s) 65
MalI GATC 4 cut(s) 270, 464, 518, 559
MbiI CCGCTC 2 cut(s) 210, 276
MboI GATC 4 cut(s) 268, 462, 516, 557
MboII GAAGA 2 cut(s) 150, 153
MflI RGATCY 3 cut(s) 462, 516, 557
MhlI GDGCHC 1 cut(s) 192
MluCI AATT 2 cut(s) 602, 615
MmeI TCCRAC 2 cut(s) 555, 585
MseI TTAA 1 cut(s) 600
MspA1I CMGCKG 2 cut(s) 375, 555
MspI CCGG 2 cut(s) 205, 512
MspR9I CCNGG 3 cut(s) 289, 431, 512
MvaI CCWGG 2 cut(s) 289, 431
MvnI CGCG 4 cut(s) 231, 325, 417, 555
MwoI GCNNNNNNNGC 1 cut(s) 324
NciI CCSGG 1 cut(s) 512
NdeII GATC 4 cut(s) 268, 462, 516, 557
NlaIII CATG 2 cut(s) 55, 448
NlaIV GGNNCC 6 cut(s) 148, 190, 191, 253, 538, 559
NmeAIII GCCGAG 1 cut(s) 369
NmuCI GTSAC 1 cut(s) 65
PaeR7I CTCGAG 1 cut(s) 167
PaqCI CACCTGC 1 cut(s) 348
PcsI WCGNNNNNNNCGW 2 cut(s) 414, 528
PfeI GAWTC 1 cut(s) 435
PflFI GACNNNGTC 1 cut(s) 283
PkrI GCNGC 4 cut(s) 212, 230, 275, 360
PpuMI RGGWCCY 1 cut(s) 251
Psp5II RGGWCCY 1 cut(s) 251
Psp6I CCWGG 2 cut(s) 287, 429
PspFI CCCAGC 1 cut(s) 539
PspGI CCWGG 2 cut(s) 287, 429
PspN4I GGNNCC 6 cut(s) 148, 190, 191, 253, 538, 559
PspOMI GGGCCC 1 cut(s) 188
PspPI GGNCC 5 cut(s) 147, 188, 189, 251, 536
PspPPI RGGWCCY 1 cut(s) 251
PspXI VCTCGAGB 1 cut(s) 167
PstNI CAGNNNCTG 1 cut(s) 340
PsuI RGATCY 3 cut(s) 462, 516, 557
PsyI GACNNNGTC 1 cut(s) 283
RsaI GTAC 1 cut(s) 611
RsaNI GTAC 1 cut(s) 610
SacII CCGCGG 1 cut(s) 556
SalI GTCGAC 1 cut(s) 506
SaqAI TTAA 1 cut(s) 600
SatI GCNGC 4 cut(s) 211, 229, 274, 359
Sau3AI GATC 4 cut(s) 268, 462, 516, 557
Sau96I GGNCC 5 cut(s) 147, 188, 189, 251, 536
ScrFI CCNGG 3 cut(s) 289, 431, 512
SduI GDGCHC 1 cut(s) 192
Sfr274I CTCGAG 1 cut(s) 167
Sfr303I CCGCGG 1 cut(s) 556
SgrBI CCGCGG 1 cut(s) 556
SinI GGWCC 2 cut(s) 147, 251
SlaI CTCGAG 1 cut(s) 167
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
Sse9I AATT 2 cut(s) 602, 615
SspI AATATT 1 cut(s) 129
StyD4I CCNGG 3 cut(s) 287, 429, 510
StyI CCWWGG 1 cut(s) 399
TaaI ACNGT 1 cut(s) 28
TaiI ACGT 3 cut(s) 284, 366, 411
TaqI TCGA 5 cut(s) 168, 278, 345, 507, 583
TaqII GACCGA 1 cut(s) 172
TasI AATT 2 cut(s) 602, 615
TauI GCSGC 4 cut(s) 213, 231, 276, 361
TfiI GAWTC 1 cut(s) 435
Tru1I TTAA 1 cut(s) 600
Tru9I TTAA 1 cut(s) 600
TscAI CASTG 1 cut(s) 31
TseFI GTSAC 1 cut(s) 65
Tsp45I GTSAC 1 cut(s) 65
TspRI CASTG 1 cut(s) 31
Tth111I GACNNNGTC 1 cut(s) 283
VpaK11BI GGWCC 2 cut(s) 147, 251
XagI CCTNNNNNAGG 1 cut(s) 53
XapI RAATTY 1 cut(s) 602
XhoI CTCGAG 1 cut(s) 167
XmiI GTMKAC 3 cut(s) 405, 420, 507
ZraI GACGTC 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.