Rh4DG150500

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0031055
Physical Location & Seq
Forward (+)
30080181 .. 30080540
360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG150500.1

Sequence Viewer

Length: 360 bp
ATGCTGACCCACTCGTCAAGGGCCGCTGCTCTCTTCCTCTTTTTGCTCCTAACGCTCACCATCCTCCTCTCCGAGTGCTCCCCTTCTCTGCTACCCCTCTCCACCGATCTCATCTTCGGCCTCACCGCCGCACTCTTCTATATCCACTCCTTCATATCTCGAGAAGCTGCCTCCGTCCAGACCTCCGATCTACAGGCCAAGTGCGAAGAATGGATTTGTTTCAGGTTGCCGTTTGATCTGAGCTCATGGCCGACCTGGACCCAACAACTTCCAATTCTTGCCGCTCCCGCCGTCGAAACTCTTCTTGATTCGGACCTTGGGATAAGGAGAGCAGGGAGGCTTAGGTTAGGAGAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

119

Amino Acids

13.22

Weight (kDa)

5.04

Isoelectric Point (pI)

56.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031055)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000268.1_g000005
rosa_samantha Rh4DG150500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 13
AccBSI CCGCTC 1 cut(s) 284
AciI CCGC 5 cut(s) 24, 126, 129, 282, 288
AcoI YGGCCR 1 cut(s) 248
AjnI CCWGG 1 cut(s) 254
AluBI AGCT 2 cut(s) 167, 243
AluI AGCT 2 cut(s) 167, 243
Alw21I GWGCWC 2 cut(s) 80, 245
Ama87I CYCGRG 1 cut(s) 159
AoxI GGCC 4 cut(s) 21, 118, 195, 248
ApeKI GCWGC 2 cut(s) 26, 167
Asp700I GAANNNNTTC 1 cut(s) 300
AspS9I GGNCC 3 cut(s) 21, 258, 313
AsuHPI GGTGA 2 cut(s) 49, 115
AvaI CYCGRG 1 cut(s) 159
AvaII GGWCC 2 cut(s) 258, 313
BanII GRGCYC 1 cut(s) 245
Bbv12I GWGCWC 2 cut(s) 80, 245
BbvI GCAGC 2 cut(s) 13, 154
BccI CCATC 1 cut(s) 68
BceAI ACGGC 2 cut(s) 214, 275
BciT130I CCWGG 1 cut(s) 256
BfmI CTRYAG 1 cut(s) 191
BisI GCNGC 5 cut(s) 24, 27, 129, 168, 282
BlsI GCNGC 5 cut(s) 25, 28, 130, 169, 283
Bme1390I CCNGG 1 cut(s) 256
Bme18I GGWCC 2 cut(s) 258, 313
BmeT110I CYCGRG 1 cut(s) 159
BmgT120I GGNCC 3 cut(s) 21, 258, 313
BmiI GGNNCC 1 cut(s) 260
BmrFI CCNGG 1 cut(s) 256
Bpu10I CCTNAGC 1 cut(s) 341
BsaJI CCNNGG 1 cut(s) 316
BseBI CCWGG 1 cut(s) 256
BseDI CCNNGG 1 cut(s) 316
BseGI GGATG 1 cut(s) 60
BseMII CTCAG 1 cut(s) 230
BseRI GAGGAG 1 cut(s) 56
BseXI GCAGC 2 cut(s) 13, 154
BshFI GGCC 4 cut(s) 23, 120, 197, 250
BsiHKAI GWGCWC 2 cut(s) 80, 245
BsiHKCI CYCGRG 1 cut(s) 159
BsnI GGCC 4 cut(s) 23, 120, 197, 250
BsoBI CYCGRG 1 cut(s) 159
Bsp1286I GDGCHC 2 cut(s) 80, 245
Bsp143I GATC 3 cut(s) 106, 187, 235
BspACI CCGC 5 cut(s) 24, 126, 129, 282, 288
BspANI GGCC 4 cut(s) 23, 120, 197, 250
BspCNI CTCAG 1 cut(s) 231
BspLI GGNNCC 1 cut(s) 260
BsrBI CCGCTC 1 cut(s) 284
BssECI CCNNGG 1 cut(s) 316
BssMI GATC 3 cut(s) 106, 187, 235
BssT1I CCWWGG 1 cut(s) 316
Bst2UI CCWGG 1 cut(s) 256
Bst6I CTCTTC 3 cut(s) 38, 140, 306
BstDEI CTNAG 2 cut(s) 239, 341
BstF5I GGATG 1 cut(s) 60
BstKTI GATC 3 cut(s) 109, 190, 238
BstMBI GATC 3 cut(s) 106, 187, 235
BstMWI GCNNNNNNNGC 2 cut(s) 52, 287
BstNI CCWGG 1 cut(s) 256
BstSCI CCNGG 1 cut(s) 254
BstSFI CTRYAG 1 cut(s) 191
BstV1I GCAGC 2 cut(s) 13, 154
BsuRI GGCC 4 cut(s) 23, 120, 197, 250
BtsCI GGATG 1 cut(s) 60
Cfr13I GGNCC 3 cut(s) 21, 258, 313
CviAII CATG 1 cut(s) 246
CviJI RGCY 7 cut(s) 23, 120, 167, 197, 243, 250, 340
CviKI_1 RGCY 7 cut(s) 23, 120, 167, 197, 243, 250, 340
DdeI CTNAG 2 cut(s) 239, 341
DpnI GATC 3 cut(s) 108, 189, 237
DpnII GATC 3 cut(s) 106, 187, 235
DrdI GACNNNNNNGTC 1 cut(s) 13
DseDI GACNNNNNNGTC 1 cut(s) 13
EaeI YGGCCR 1 cut(s) 248
Eam1104I CTCTTC 3 cut(s) 38, 140, 306
EarI CTCTTC 3 cut(s) 38, 140, 306
Ecl136II GAGCTC 1 cut(s) 243
Eco130I CCWWGG 1 cut(s) 316
Eco24I GRGCYC 1 cut(s) 245
Eco47I GGWCC 2 cut(s) 258, 313
Eco53kI GAGCTC 1 cut(s) 243
Eco88I CYCGRG 1 cut(s) 159
EcoICRI GAGCTC 1 cut(s) 243
EcoRII CCWGG 1 cut(s) 254
EcoT14I CCWWGG 1 cut(s) 316
EcoT38I GRGCYC 1 cut(s) 245
ErhI CCWWGG 1 cut(s) 316
FaeI CATG 1 cut(s) 249
FaiI YATR 3 cut(s) 141, 155, 247
FatI CATG 1 cut(s) 245
FauI CCCGC 1 cut(s) 295
Fnu4HI GCNGC 5 cut(s) 24, 27, 129, 168, 282
FokI GGATG 1 cut(s) 47
FriOI GRGCYC 1 cut(s) 245
Fsp4HI GCNGC 5 cut(s) 24, 27, 129, 168, 282
GluI GCNGC 5 cut(s) 24, 27, 129, 168, 282
HaeIII GGCC 4 cut(s) 23, 120, 197, 250
Hin1II CATG 1 cut(s) 249
HinfI GANTC 1 cut(s) 308
HphI GGTGA 2 cut(s) 49, 115
Hpy188I TCNGA 4 cut(s) 73, 187, 240, 313
Hpy188III TCNNGA 4 cut(s) 159, 161, 178, 305
Hpy99I CGWCG 1 cut(s) 296
HpyAV CCTTC 2 cut(s) 93, 160
HpyF10VI GCNNNNNNNGC 2 cut(s) 52, 287
HpyF3I CTNAG 2 cut(s) 239, 341
Hsp92II CATG 1 cut(s) 249
Kzo9I GATC 3 cut(s) 106, 187, 235
LmnI GCTCC 3 cut(s) 51, 83, 289
LpnPI CCDG 6 cut(s) 179, 191, 208, 241, 268, 318
Lsp1109I GCAGC 2 cut(s) 13, 154
MalI GATC 3 cut(s) 108, 189, 237
MbiI CCGCTC 1 cut(s) 284
MboI GATC 3 cut(s) 106, 187, 235
MboII GAAGA 5 cut(s) 25, 106, 127, 218, 293
MhlI GDGCHC 2 cut(s) 80, 245
MluCI AATT 1 cut(s) 273
MnlI CCTC 9 cut(s) 47, 74, 77, 107, 131, 181, 193, 330, 346
MroXI GAANNNNTTC 1 cut(s) 300
MspA1I CMGCKG 1 cut(s) 26
MspR9I CCNGG 1 cut(s) 256
MvaI CCWGG 1 cut(s) 256
MwoI GCNNNNNNNGC 2 cut(s) 52, 287
NdeII GATC 3 cut(s) 106, 187, 235
NlaIII CATG 1 cut(s) 249
NlaIV GGNNCC 1 cut(s) 260
PaeR7I CTCGAG 1 cut(s) 159
PdmI GAANNNNTTC 1 cut(s) 300
PfeI GAWTC 1 cut(s) 308
PkrI GCNGC 5 cut(s) 25, 28, 130, 169, 283
Psp124BI GAGCTC 1 cut(s) 245
Psp6I CCWGG 1 cut(s) 254
PspGI CCWGG 1 cut(s) 254
PspN4I GGNNCC 1 cut(s) 260
PspPI GGNCC 3 cut(s) 21, 258, 313
SacI GAGCTC 1 cut(s) 245
SatI GCNGC 5 cut(s) 24, 27, 129, 168, 282
Sau3AI GATC 3 cut(s) 106, 187, 235
Sau96I GGNCC 3 cut(s) 21, 258, 313
ScrFI CCNGG 1 cut(s) 256
SduI GDGCHC 2 cut(s) 80, 245
SetI ASST 7 cut(s) 169, 185, 227, 245, 257, 318, 347
SfcI CTRYAG 1 cut(s) 191
Sfr274I CTCGAG 1 cut(s) 159
SinI GGWCC 2 cut(s) 258, 313
SlaI CTCGAG 1 cut(s) 159
SmlI CTYRAG 1 cut(s) 159
SmoI CTYRAG 1 cut(s) 159
Sse9I AATT 1 cut(s) 273
SsiI CCGC 5 cut(s) 24, 126, 129, 282, 288
SstI GAGCTC 1 cut(s) 245
StyD4I CCNGG 1 cut(s) 254
StyI CCWWGG 1 cut(s) 316
TaqI TCGA 2 cut(s) 160, 294
TasI AATT 1 cut(s) 273
TauI GCSGC 3 cut(s) 26, 131, 284
TfiI GAWTC 1 cut(s) 308
TseI GCWGC 2 cut(s) 26, 167
TspDTI ATGAA 1 cut(s) 142
TspGWI ACGGA 1 cut(s) 163
VpaK11BI GGWCC 2 cut(s) 258, 313
XhoI CTCGAG 1 cut(s) 159
XmnI GAANNNNTTC 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.