Rh4DG386000

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0030110
Physical Location & Seq
Reverse (-)
60302674 .. 60305518
2845 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG386000.1

Sequence Viewer

Length: 855 bp
ATGCTTCACGTCTTGGTTTGTTCACTTCAGTGCAGAGATTATTGGTTTAATGCGGCTTGCAAGCCCAATAAACAAGACAGCCACAAAGTACAAAAGGAGAGGGAATGCACAAGCCCAACTCCACATATTGTCATGATGAGCTCTTTGAGATGCATAGCAAAACACCCAACAATTGCACTAGTAGACTCATCCACTACTCTCAAAGATCTCAAGCAAATCCACACCCAACTCCTTAATAACGGCGTCCTCAACGATCCTCACCACTCCGGCAATTTTGTTGCCACCGTTGCCGTCCGGAACCCCAACAATTTGGAATATTCCAACCGAATTCTGGACCAATGTGACAACCCAACTCTCTTCGCCTTCAACTCCATGATCAGGGCCTGTTCCAAATGCTCCGCACCCACTAAAAGCTTTCACTTTTACAGCAGAATCCTCCATTCACGTAACAATCTTCTCCCCGATAACTACACATTCAACTTCTTGGTTCGCACTTCTGCGCAGCTTTCGTCATTTGGGACTGGTCCTTCGGTCCATGGTGCATTGGTGAGGCACGGGTTTGAGAATGACCCGCATGTTCAAAGTGCTTTAATATTTATGTATGCGGAATTGGGTTGCTTGGAGCAATGTCATGGAGTGTTTAGCAGCACTGCCGAGCCTGACTTGGTGTGTCAAACTGCTATTGTGAGCGCTTGTGCCAAATGTGGGGATGTAGATTATGCACGGCAATTGTTCGATGAAATGCCTGAGAGAGACCCCATTGCTTGGAATGCGATGATTGCGGGGTACGCGCAATGTGGGAAGTCCAGGGAAGCTTTGGGTTTGTTCCATTTGATGCAAATGGAGGGTGAATGA

Protein Analysis

284

Amino Acids

31.68

Weight (kDa)

6.69

Isoelectric Point (pI)

41.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 255 - 283 5e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030110)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0440731
rosa_samantha Rh4DG386000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 501
AccB7I CCANNNNNTGG 1 cut(s) 765
AccI GTMKAC 1 cut(s) 183
AccII CGCG 1 cut(s) 791
AccIII TCCGGA 1 cut(s) 294
AciI CCGC 5 cut(s) 53, 399, 572, 605, 782
AclWI GGATC 1 cut(s) 248
AcsI RAATTY 1 cut(s) 327
AcuI CTGAAG 1 cut(s) 11
AcyI GRCGYC 1 cut(s) 243
AfaI GTAC 2 cut(s) 90, 788
AfeI AGCGCT 1 cut(s) 691
AfiI CCNNNNNNNGG 4 cut(s) 331, 378, 705, 765
AgsI TTSAA 3 cut(s) 367, 478, 581
AhlI ACTAGT 1 cut(s) 178
AjiI CACGTC 1 cut(s) 10
AjnI CCWGG 1 cut(s) 806
AleI CACNNNNGTG 1 cut(s) 28
AluBI AGCT 4 cut(s) 141, 414, 505, 815
AluI AGCT 4 cut(s) 141, 414, 505, 815
Alw21I GWGCWC 1 cut(s) 143
Alw26I GTCTC 1 cut(s) 747
AlwI GGATC 1 cut(s) 248
AlwNI CAGNNNCTG 1 cut(s) 384
Aor13HI TCCGGA 1 cut(s) 294
Aor51HI AGCGCT 1 cut(s) 691
AoxI GGCC 1 cut(s) 381
ApeKI GCWGC 2 cut(s) 502, 645
ApoI RAATTY 1 cut(s) 327
ArsI GACNNNNNNTTYG 2 cut(s) 511, 543
AspLEI GCGC 3 cut(s) 502, 692, 793
AspS9I GGNCC 4 cut(s) 334, 381, 524, 532
AsuHPI GGTGA 2 cut(s) 251, 559
AvaII GGWCC 3 cut(s) 334, 524, 532
BanII GRGCYC 1 cut(s) 143
Bbv12I GWGCWC 1 cut(s) 143
BbvI GCAGC 2 cut(s) 514, 657
BceAI ACGGC 3 cut(s) 256, 275, 740
BciT130I CCWGG 1 cut(s) 808
BclI TGATCA 1 cut(s) 375
BcoDI GTCTC 1 cut(s) 747
BcuI ACTAGT 1 cut(s) 178
BfaI CTAG 1 cut(s) 179
BfoI RGCGCY 1 cut(s) 693
BglII AGATCT 1 cut(s) 205
BisI GCNGC 3 cut(s) 54, 503, 646
BlsI GCNGC 3 cut(s) 55, 504, 647
Bme1390I CCNGG 1 cut(s) 808
Bme18I GGWCC 3 cut(s) 334, 524, 532
BmgBI CACGTC 1 cut(s) 10
BmgT120I GGNCC 4 cut(s) 334, 381, 524, 532
BmiI GGNNCC 1 cut(s) 299
BmrFI CCNGG 1 cut(s) 808
BmsI GCATC 2 cut(s) 140, 825
BpuEI CTTGAG 1 cut(s) 194
BsaAI YACGTR 1 cut(s) 446
BsaHI GRCGYC 1 cut(s) 243
BsaI GGTCTC 1 cut(s) 747
BsaJI CCNNGG 2 cut(s) 535, 807
BsaWI WCCGGW 1 cut(s) 294
Bsc4I CCNNNNNNNGG 4 cut(s) 331, 378, 705, 765
Bse1I ACTGG 1 cut(s) 526
Bse3DI GCAATG 3 cut(s) 632, 759, 800
BseAI TCCGGA 1 cut(s) 294
BseBI CCWGG 1 cut(s) 808
BseDI CCNNGG 2 cut(s) 535, 807
BseGI GGATG 2 cut(s) 188, 715
BseLI CCNNNNNNNGG 4 cut(s) 331, 378, 705, 765
BseMI GCAATG 3 cut(s) 632, 759, 800
BseMII CTCAG 1 cut(s) 738
BseNI ACTGG 1 cut(s) 526
BseXI GCAGC 2 cut(s) 514, 657
BsgI GTGCAG 1 cut(s) 52
Bsh1236I CGCG 1 cut(s) 791
BshFI GGCC 1 cut(s) 383
BsiHKAI GWGCWC 1 cut(s) 143
BsiSI CCGG 2 cut(s) 267, 295
BslFI GGGAC 1 cut(s) 532
BslI CCNNNNNNNGG 4 cut(s) 331, 378, 705, 765
BsmAI GTCTC 1 cut(s) 747
BsmFI GGGAC 1 cut(s) 532
BsmI GAATGC 2 cut(s) 110, 775
BsnI GGCC 1 cut(s) 383
Bso31I GGTCTC 1 cut(s) 747
Bsp1286I GDGCHC 1 cut(s) 143
Bsp13I TCCGGA 1 cut(s) 294
Bsp143I GATC 3 cut(s) 205, 253, 375
Bsp19I CCATGG 1 cut(s) 535
BspACI CCGC 5 cut(s) 53, 399, 572, 605, 782
BspANI GGCC 1 cut(s) 383
BspCNI CTCAG 1 cut(s) 739
BspEI TCCGGA 1 cut(s) 294
BspFNI CGCG 1 cut(s) 791
BspHI TCATGA 1 cut(s) 132
BspLI GGNNCC 1 cut(s) 299
BspPI GGATC 1 cut(s) 248
BspTNI GGTCTC 1 cut(s) 747
BsrDI GCAATG 3 cut(s) 632, 759, 800
BsrI ACTGG 1 cut(s) 526
BssECI CCNNGG 2 cut(s) 535, 807
BssMI GATC 3 cut(s) 205, 253, 375
BssNI GRCGYC 1 cut(s) 243
BssT1I CCWWGG 1 cut(s) 535
Bst2UI CCWGG 1 cut(s) 808
Bst4CI ACNGT 1 cut(s) 286
Bst6I CTCTTC 1 cut(s) 362
BstACI GRCGYC 1 cut(s) 243
BstBAI YACGTR 1 cut(s) 446
BstC8I GCNNGC 2 cut(s) 58, 62
BstDEI CTNAG 1 cut(s) 747
BstDSI CCRYGG 1 cut(s) 535
BstF5I GGATG 2 cut(s) 188, 715
BstFNI CGCG 1 cut(s) 791
BstH2I RGCGCY 1 cut(s) 693
BstHHI GCGC 3 cut(s) 502, 692, 793
BstKTI GATC 3 cut(s) 208, 256, 378
BstMAI GTCTC 1 cut(s) 747
BstMBI GATC 3 cut(s) 205, 253, 375
BstMWI GCNNNNNNNGC 4 cut(s) 287, 770, 779, 788
BstNI CCWGG 1 cut(s) 808
BstNSI RCATGY 1 cut(s) 578
BstSCI CCNGG 1 cut(s) 806
BstUI CGCG 1 cut(s) 791
BstV1I GCAGC 2 cut(s) 514, 657
BstX2I RGATCY 1 cut(s) 205
BstXI CCANNNNNNTGG 1 cut(s) 310
BstYI RGATCY 1 cut(s) 205
BsuRI GGCC 1 cut(s) 383
BtgI CCRYGG 1 cut(s) 535
BtgZI GCGATG 1 cut(s) 788
BtrI CACGTC 1 cut(s) 10
BtsCI GGATG 2 cut(s) 188, 715
BtsI GCAGTG 1 cut(s) 648
BtsIMutI CAGTG 2 cut(s) 35, 648
Cac8I GCNNGC 2 cut(s) 58, 62
CaiI CAGNNNCTG 1 cut(s) 384
CciI TCATGA 1 cut(s) 132
CfoI GCGC 3 cut(s) 502, 692, 793
Cfr13I GGNCC 4 cut(s) 334, 381, 524, 532
CseI GACGC 1 cut(s) 232
Csp6I GTAC 2 cut(s) 89, 787
CviAII CATG 5 cut(s) 133, 373, 536, 575, 632
CviQI GTAC 2 cut(s) 89, 787
DdeI CTNAG 1 cut(s) 747
DpnI GATC 3 cut(s) 207, 255, 377
DpnII GATC 3 cut(s) 205, 253, 375
Eam1104I CTCTTC 1 cut(s) 362
EarI CTCTTC 1 cut(s) 362
Ecl136II GAGCTC 1 cut(s) 141
Eco130I CCWWGG 1 cut(s) 535
Eco24I GRGCYC 1 cut(s) 143
Eco31I GGTCTC 1 cut(s) 747
Eco47I GGWCC 3 cut(s) 334, 524, 532
Eco47III AGCGCT 1 cut(s) 691
Eco53kI GAGCTC 1 cut(s) 141
Eco57I CTGAAG 1 cut(s) 11
EcoICRI GAGCTC 1 cut(s) 141
EcoO109I RGGNCCY 1 cut(s) 381
EcoRI GAATTC 1 cut(s) 327
EcoRII CCWGG 1 cut(s) 806
EcoT14I CCWWGG 1 cut(s) 535
EcoT22I ATGCAT 1 cut(s) 155
EcoT38I GRGCYC 1 cut(s) 143
ErhI CCWWGG 1 cut(s) 535
FaeI CATG 5 cut(s) 136, 376, 539, 578, 635
FaqI GGGAC 1 cut(s) 532
FatI CATG 5 cut(s) 132, 372, 535, 574, 631
FauI CCCGC 2 cut(s) 579, 775
FbaI TGATCA 1 cut(s) 375
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 3 cut(s) 54, 503, 646
FokI GGATG 2 cut(s) 175, 722
FriOI GRGCYC 1 cut(s) 143
Fsp4HI GCNGC 3 cut(s) 54, 503, 646
FspBI CTAG 1 cut(s) 179
FspI TGCGCA 1 cut(s) 501
GlaI GCGC 3 cut(s) 501, 691, 792
GluI GCNGC 3 cut(s) 54, 503, 646
HaeII RGCGCY 1 cut(s) 693
HaeIII GGCC 1 cut(s) 383
HapII CCGG 2 cut(s) 267, 295
HgaI GACGC 1 cut(s) 232
HhaI GCGC 3 cut(s) 502, 692, 793
Hin1I GRCGYC 1 cut(s) 243
Hin1II CATG 5 cut(s) 136, 376, 539, 578, 635
Hin6I GCGC 3 cut(s) 500, 690, 791
HinP1I GCGC 3 cut(s) 500, 690, 791
HindIII AAGCTT 2 cut(s) 412, 813
HinfI GANTC 2 cut(s) 185, 432
HpaII CCGG 2 cut(s) 267, 295
HphI GGTGA 2 cut(s) 251, 559
Hpy166II GTNNAC 2 cut(s) 23, 184
Hpy188III TCNNGA 3 cut(s) 133, 295, 332
Hpy8I GTNNAC 2 cut(s) 23, 184
HpyAV CCTTC 2 cut(s) 373, 537
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4IV ACGT 2 cut(s) 9, 445
HpyCH4V TGCA 8 cut(s) 33, 60, 108, 153, 176, 542, 722, 838
HpyF10VI GCNNNNNNNGC 4 cut(s) 287, 770, 779, 788
HpyF3I CTNAG 1 cut(s) 747
HpySE526I ACGT 2 cut(s) 9, 445
Hsp92I GRCGYC 1 cut(s) 243
Hsp92II CATG 5 cut(s) 136, 376, 539, 578, 635
HspAI GCGC 3 cut(s) 500, 690, 791
Kpn2I TCCGGA 1 cut(s) 294
Ksp22I TGATCA 1 cut(s) 375
Kzo9I GATC 3 cut(s) 205, 253, 375
LmnI GCTCC 2 cut(s) 401, 622
Lsp1109I GCAGC 2 cut(s) 514, 657
LweI GCATC 2 cut(s) 140, 825
MaeI CTAG 1 cut(s) 179
MaeII ACGT 2 cut(s) 9, 445
MaeIII GTNAC 2 cut(s) 341, 446
MalI GATC 3 cut(s) 207, 255, 377
MboI GATC 3 cut(s) 205, 253, 375
MboII GAAGA 2 cut(s) 349, 446
MfeI CAATTG 2 cut(s) 171, 728
MflI RGATCY 1 cut(s) 205
MhlI GDGCHC 1 cut(s) 143
MluCI AATT 6 cut(s) 171, 271, 307, 327, 608, 728
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 1 cut(s) 345
MnlI CCTC 6 cut(s) 93, 257, 267, 446, 543, 838
Mph1103I ATGCAT 1 cut(s) 155
MroI TCCGGA 1 cut(s) 294
MseI TTAA 3 cut(s) 48, 234, 590
MslI CAYNNNNRTG 1 cut(s) 28
MspI CCGG 2 cut(s) 267, 295
MspR9I CCNGG 1 cut(s) 808
MunI CAATTG 2 cut(s) 171, 728
Mva1269I GAATGC 2 cut(s) 110, 775
MvaI CCWGG 1 cut(s) 808
MvnI CGCG 1 cut(s) 791
MwoI GCNNNNNNNGC 4 cut(s) 287, 770, 779, 788
NcoI CCATGG 1 cut(s) 535
NdeII GATC 3 cut(s) 205, 253, 375
NlaIII CATG 5 cut(s) 136, 376, 539, 578, 635
NlaIV GGNNCC 1 cut(s) 299
NmeAIII GCCGAG 1 cut(s) 679
NmuCI GTSAC 1 cut(s) 341
NsbI TGCGCA 1 cut(s) 501
NsiI ATGCAT 1 cut(s) 155
NspI RCATGY 1 cut(s) 578
OliI CACNNNNGTG 1 cut(s) 28
PagI TCATGA 1 cut(s) 132
PctI GAATGC 2 cut(s) 110, 775
PfeI GAWTC 1 cut(s) 432
PflMI CCANNNNNTGG 1 cut(s) 765
PkrI GCNGC 3 cut(s) 55, 504, 647
PleI GAGTC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 179
Ppu21I YACGTR 1 cut(s) 446
Psp124BI GAGCTC 1 cut(s) 143
Psp6I CCWGG 1 cut(s) 806
PspGI CCWGG 1 cut(s) 806
PspN4I GGNNCC 1 cut(s) 299
PspPI GGNCC 4 cut(s) 334, 381, 524, 532
PstNI CAGNNNCTG 1 cut(s) 384
PsuI RGATCY 1 cut(s) 205
RsaI GTAC 2 cut(s) 90, 788
RsaNI GTAC 2 cut(s) 89, 787
RseI CAYNNNNRTG 1 cut(s) 28
SacI GAGCTC 1 cut(s) 143
SaqAI TTAA 3 cut(s) 48, 234, 590
SatI GCNGC 3 cut(s) 54, 503, 646
Sau3AI GATC 3 cut(s) 205, 253, 375
Sau96I GGNCC 4 cut(s) 334, 381, 524, 532
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 1 cut(s) 808
SduI GDGCHC 1 cut(s) 143
SetI ASST 6 cut(s) 12, 143, 416, 448, 507, 817
SfaNI GCATC 2 cut(s) 140, 825
SinI GGWCC 3 cut(s) 334, 524, 532
SmiMI CAYNNNNRTG 1 cut(s) 28
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
SpeI ACTAGT 1 cut(s) 178
Sse9I AATT 6 cut(s) 171, 271, 307, 327, 608, 728
SsiI CCGC 5 cut(s) 53, 399, 572, 605, 782
SspI AATATT 2 cut(s) 317, 594
SspMI CTAG 1 cut(s) 179
SstI GAGCTC 1 cut(s) 143
StyD4I CCNGG 1 cut(s) 806
StyI CCWWGG 1 cut(s) 535
TaaI ACNGT 1 cut(s) 286
TaiI ACGT 2 cut(s) 12, 448
TaqI TCGA 1 cut(s) 735
TaqII GACCGA 1 cut(s) 520
TasI AATT 6 cut(s) 171, 271, 307, 327, 608, 728
TatI WGTACW 1 cut(s) 88
TauI GCSGC 1 cut(s) 56
TfiI GAWTC 1 cut(s) 432
Tru1I TTAA 3 cut(s) 48, 234, 590
Tru9I TTAA 3 cut(s) 48, 234, 590
TscAI CASTG 2 cut(s) 35, 655
TseFI GTSAC 1 cut(s) 341
TseI GCWGC 2 cut(s) 502, 645
Tsp45I GTSAC 1 cut(s) 341
TspDTI ATGAA 1 cut(s) 753
TspRI CASTG 2 cut(s) 35, 655
Van91I CCANNNNNTGG 1 cut(s) 765
VpaK11BI GGWCC 3 cut(s) 334, 524, 532
XapI RAATTY 1 cut(s) 327
XceI RCATGY 1 cut(s) 578
XcmI CCANNNNNNNNNTGG 2 cut(s) 328, 814
XmiI GTMKAC 1 cut(s) 183
XspI CTAG 1 cut(s) 179
Zsp2I ATGCAT 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.