Rh5AG106000

Belongs to the MCM family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0029285
Physical Location & Seq
Forward (+)
9706382 .. 9707043
662 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG106000.1

Sequence Viewer

Length: 483 bp
ATGGTGGCTAATGCATTGGCAGGTTCTCTTGTGAGGCCAAAAGTTGTTAAGAGTGTTCATTTCTGCCCTACAACCGGAGACATTACATCCAACATGGGTTTGCCTACAGGAACAGTTTATCCCACCAGGACTTTGTCAATGCAAGAAGTGCCTGAAAACTCTGCTCCTGGTCAGCTCCCAAGAACAGTGGATGTCATAGTTGAGGATGGCCTTGTAGATAAGTGCAAACCAGGAGACCGTGTGGCTGTTGTAGGGATTTACAAAGCTCTTCCTGGTAAAAGTAAGGGAAGTGTGAACGGAGTATTCAGGACGGTTCTCATAGCTAACAATGTTACTCAGCTCAACAAAGAGGCACAATCGCCACAGTACAGTGCTGAAGACCTGACGAATATAAAAAATATAGCAGAGAGAGATGATGCATTTGATCTGCTTGGCAATTCACTTGCACCTTCTATTTATGGGCATTCAGCCATTCATGGATAA

Protein Analysis

160

Amino Acids

16.92

Weight (kDa)

6.82

Isoelectric Point (pI)

25.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCM_OB PF17207 39 - 90 1.6e-14 MCM OB domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0029285)

Species Orthologous Gene IDs
pyrus_communis pycom05g26500
rosa_samantha Rh5AG106000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 11
AcuI CTGAAG 1 cut(s) 396
AfaI GTAC 1 cut(s) 368
AfiI CCNNNNNNNGG 1 cut(s) 74
AjnI CCWGG 4 cut(s) 125, 166, 229, 271
AloI GAACNNNNNNTCC 2 cut(s) 103, 135
AluBI AGCT 4 cut(s) 175, 266, 323, 340
AluI AGCT 4 cut(s) 175, 266, 323, 340
Alw26I GTCTC 2 cut(s) 72, 228
AoxI GGCC 2 cut(s) 35, 208
BbsI GAAGAC 1 cut(s) 384
BccI CCATC 1 cut(s) 200
BciT130I CCWGG 4 cut(s) 127, 168, 231, 273
BcoDI GTCTC 2 cut(s) 72, 228
BfmI CTRYAG 1 cut(s) 105
BfuAI ACCTGC 1 cut(s) 11
Bme1390I CCNGG 4 cut(s) 127, 168, 231, 273
BmrFI CCNGG 4 cut(s) 127, 168, 231, 273
BmsI GCATC 1 cut(s) 406
BpiI GAAGAC 1 cut(s) 384
BsaI GGTCTC 1 cut(s) 228
BsaWI WCCGGW 1 cut(s) 74
BsaXI ACNNNNNCTCC 2 cut(s) 225, 255
Bsc4I CCNNNNNNNGG 1 cut(s) 74
BseBI CCWGG 4 cut(s) 127, 168, 231, 273
BseGI GGATG 3 cut(s) 86, 196, 211
BseLI CCNNNNNNNGG 1 cut(s) 74
BseMII CTCAG 1 cut(s) 350
BshFI GGCC 2 cut(s) 37, 210
BsiSI CCGG 1 cut(s) 75
BslI CCNNNNNNNGG 1 cut(s) 74
BsmAI GTCTC 2 cut(s) 72, 228
BsmI GAATGC 1 cut(s) 463
BsnI GGCC 2 cut(s) 37, 210
Bso31I GGTCTC 1 cut(s) 228
Bsp143I GATC 1 cut(s) 424
BspANI GGCC 2 cut(s) 37, 210
BspCNI CTCAG 1 cut(s) 349
BspMI ACCTGC 1 cut(s) 11
BspQI GCTCTTC 1 cut(s) 273
BspTNI GGTCTC 1 cut(s) 228
BssMI GATC 1 cut(s) 424
Bst2UI CCWGG 4 cut(s) 127, 168, 231, 273
Bst4CI ACNGT 6 cut(s) 115, 187, 239, 313, 366, 371
Bst6I CTCTTC 1 cut(s) 273
BstAPI GCANNNNNTGC 1 cut(s) 148
BstDEI CTNAG 1 cut(s) 336
BstF5I GGATG 3 cut(s) 86, 196, 211
BstKTI GATC 1 cut(s) 427
BstMAI GTCTC 2 cut(s) 72, 228
BstMBI GATC 1 cut(s) 424
BstMWI GCNNNNNNNGC 1 cut(s) 148
BstNI CCWGG 4 cut(s) 127, 168, 231, 273
BstSCI CCNGG 4 cut(s) 125, 166, 229, 271
BstSFI CTRYAG 1 cut(s) 105
BstV2I GAAGAC 1 cut(s) 384
BsuRI GGCC 2 cut(s) 37, 210
BtsCI GGATG 3 cut(s) 86, 196, 211
BtsIMutI CAGTG 2 cut(s) 192, 376
BveI ACCTGC 1 cut(s) 11
Csp6I GTAC 1 cut(s) 367
CspCI CAANNNNNGTGG 2 cut(s) 168, 203
CviAII CATG 2 cut(s) 94, 476
CviJI RGCY 9 cut(s) 8, 37, 175, 210, 245, 266, 323, 340, 470
CviKI_1 RGCY 9 cut(s) 8, 37, 175, 210, 245, 266, 323, 340, 470
CviQI GTAC 1 cut(s) 367
DdeI CTNAG 1 cut(s) 336
DpnI GATC 1 cut(s) 426
DpnII GATC 1 cut(s) 424
Eam1104I CTCTTC 1 cut(s) 273
EarI CTCTTC 1 cut(s) 273
Eco31I GGTCTC 1 cut(s) 228
Eco57I CTGAAG 1 cut(s) 396
EcoRII CCWGG 4 cut(s) 125, 166, 229, 271
EcoT22I ATGCAT 2 cut(s) 16, 421
FaeI CATG 2 cut(s) 97, 479
FaiI YATR 7 cut(s) 95, 197, 320, 392, 401, 459, 477
FatI CATG 2 cut(s) 93, 475
FokI GGATG 3 cut(s) 73, 203, 218
HaeIII GGCC 2 cut(s) 37, 210
HapII CCGG 1 cut(s) 75
Hin1II CATG 2 cut(s) 97, 479
HpaII CCGG 1 cut(s) 75
Hpy166II GTNNAC 1 cut(s) 295
Hpy188III TCNNGA 1 cut(s) 307
Hpy8I GTNNAC 1 cut(s) 295
HpyAV CCTTC 1 cut(s) 459
HpyCH4III ACNGT 6 cut(s) 115, 187, 239, 313, 366, 371
HpyCH4V TGCA 5 cut(s) 14, 142, 225, 419, 446
HpyF10VI GCNNNNNNNGC 1 cut(s) 148
HpyF3I CTNAG 1 cut(s) 336
Hsp92II CATG 2 cut(s) 97, 479
Kzo9I GATC 1 cut(s) 424
LguI GCTCTTC 1 cut(s) 273
LmnI GCTCC 2 cut(s) 169, 180
LweI GCATC 1 cut(s) 406
MaeIII GTNAC 1 cut(s) 331
MalI GATC 1 cut(s) 426
MboI GATC 1 cut(s) 424
MboII GAAGA 2 cut(s) 260, 389
MluCI AATT 1 cut(s) 436
MmeI TCCRAC 1 cut(s) 114
MnlI CCTC 3 cut(s) 27, 196, 343
Mph1103I ATGCAT 2 cut(s) 16, 421
MseI TTAA 1 cut(s) 48
MspI CCGG 1 cut(s) 75
MspR9I CCNGG 4 cut(s) 127, 168, 231, 273
Mva1269I GAATGC 1 cut(s) 463
MvaI CCWGG 4 cut(s) 127, 168, 231, 273
MwoI GCNNNNNNNGC 1 cut(s) 148
NdeII GATC 1 cut(s) 424
NlaIII CATG 2 cut(s) 97, 479
NsiI ATGCAT 2 cut(s) 16, 421
PciSI GCTCTTC 1 cut(s) 273
PctI GAATGC 1 cut(s) 463
PflFI GACNNNGTC 1 cut(s) 133
Psp6I CCWGG 4 cut(s) 125, 166, 229, 271
PspGI CCWGG 4 cut(s) 125, 166, 229, 271
PsyI GACNNNGTC 1 cut(s) 133
RsaI GTAC 1 cut(s) 368
RsaNI GTAC 1 cut(s) 367
SapI GCTCTTC 1 cut(s) 273
SaqAI TTAA 1 cut(s) 48
Sau3AI GATC 1 cut(s) 424
ScrFI CCNGG 4 cut(s) 127, 168, 231, 273
SetI ASST 7 cut(s) 25, 177, 268, 325, 342, 384, 451
SfaNI GCATC 1 cut(s) 406
SfcI CTRYAG 1 cut(s) 105
Sse9I AATT 1 cut(s) 436
StyD4I CCNGG 4 cut(s) 125, 166, 229, 271
TaaI ACNGT 6 cut(s) 115, 187, 239, 313, 366, 371
TasI AATT 1 cut(s) 436
TatI WGTACW 1 cut(s) 366
Tru1I TTAA 1 cut(s) 48
Tru9I TTAA 1 cut(s) 48
TscAI CASTG 2 cut(s) 192, 376
TspDTI ATGAA 2 cut(s) 47, 464
TspGWI ACGGA 1 cut(s) 312
TspRI CASTG 2 cut(s) 192, 376
Tth111I GACNNNGTC 1 cut(s) 133
Zsp2I ATGCAT 2 cut(s) 16, 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.