Rh5AG202000

Polycomb group protein EMBRYONIC FLOWER

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0022437
Physical Location & Seq
Forward (+)
23650824 .. 23652665
1842 bp
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UTR
Exon/CDS
Intron
Rh5AG202000.1

Sequence Viewer

Length: 291 bp
ATGCTTGATGATTTCGTTGATGTAACCAAAGATGAGAAGCAAATGATGCATATGTGGAACTCCTTCGTGAGAAAGCAACAGGTGTTGGCTGATGGCCACATTCCTTGGGCGTGTGAGGCATTTTCAAGATTGCATGCACATGAGCTTGTGCAATCCCCGGCCTTGATATGGTGTTGGAGATTATTTATGATCAAGTTGTGGAATCATGGTCTCCTGGATGCACGCAGTATGAACAACTGTAACATTATTCTTGAACAATATCCGAGCAAGGTTTTGGATCCGAAGAGTTGA

Protein Analysis

96

Amino Acids

11.37

Weight (kDa)

6.49

Isoelectric Point (pI)

56.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VEFS-Box PF09733 1 - 77 2.4e-34 VEFS-Box of polycomb protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022437)

Species Orthologous Gene IDs
malus_domestica MD11G1274400.v1.1
rosa_multiflora Rmu_sc0003221.1_g000059
rosa_samantha Rh5AG202000 Rh5CG220500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 272, 285
AcoI YGGCCR 1 cut(s) 94
AfiI CCNNNNNNNGG 1 cut(s) 168
AgsI TTSAA 2 cut(s) 126, 254
AjnI CCWGG 1 cut(s) 213
AluBI AGCT 1 cut(s) 145
AluI AGCT 1 cut(s) 145
Alw26I GTCTC 1 cut(s) 215
AlwI GGATC 2 cut(s) 272, 285
AoxI GGCC 2 cut(s) 94, 159
Asp700I GAANNNNTTC 1 cut(s) 62
AsuC2I CCSGG 1 cut(s) 158
BalI TGGCCA 1 cut(s) 96
BamHI GGATCC 1 cut(s) 277
BccI CCATC 1 cut(s) 86
BciT130I CCWGG 1 cut(s) 215
BclI TGATCA 1 cut(s) 189
BcnI CCSGG 1 cut(s) 158
BcoDI GTCTC 1 cut(s) 215
Bme1390I CCNGG 2 cut(s) 158, 215
BmiI GGNNCC 1 cut(s) 279
BmrFI CCNGG 2 cut(s) 158, 215
BmsI GCATC 2 cut(s) 36, 208
BpuMI CCSGG 1 cut(s) 158
BsaI GGTCTC 1 cut(s) 215
BsaJI CCNNGG 2 cut(s) 104, 156
Bsc4I CCNNNNNNNGG 1 cut(s) 168
BseBI CCWGG 1 cut(s) 215
BseDI CCNNGG 2 cut(s) 104, 156
BseGI GGATG 1 cut(s) 223
BseLI CCNNNNNNNGG 1 cut(s) 168
BshFI GGCC 2 cut(s) 96, 161
BsiSI CCGG 1 cut(s) 158
BslI CCNNNNNNNGG 1 cut(s) 168
BsmAI GTCTC 1 cut(s) 215
BsnI GGCC 2 cut(s) 96, 161
Bso31I GGTCTC 1 cut(s) 215
Bsp143I GATC 2 cut(s) 189, 277
BspANI GGCC 2 cut(s) 96, 161
BspLI GGNNCC 1 cut(s) 279
BspPI GGATC 2 cut(s) 272, 285
BspTNI GGTCTC 1 cut(s) 215
BssECI CCNNGG 2 cut(s) 104, 156
BssMI GATC 2 cut(s) 189, 277
BssT1I CCWWGG 1 cut(s) 104
Bst2UI CCWGG 1 cut(s) 215
Bst4CI ACNGT 1 cut(s) 239
Bst6I CTCTTC 1 cut(s) 278
BstAPI GCANNNNNTGC 1 cut(s) 46
BstC8I GCNNGC 2 cut(s) 135, 223
BstF5I GGATG 1 cut(s) 223
BstKTI GATC 2 cut(s) 192, 280
BstMAI GTCTC 1 cut(s) 215
BstMBI GATC 2 cut(s) 189, 277
BstMWI GCNNNNNNNGC 2 cut(s) 46, 116
BstNI CCWGG 1 cut(s) 215
BstNSI RCATGY 1 cut(s) 137
BstSCI CCNGG 2 cut(s) 156, 213
BstX2I RGATCY 1 cut(s) 277
BstYI RGATCY 1 cut(s) 277
BsuRI GGCC 2 cut(s) 96, 161
BtsCI GGATG 1 cut(s) 223
Cac8I GCNNGC 2 cut(s) 135, 223
CspCI CAANNNNNGTGG 2 cut(s) 86, 121
CviAII CATG 3 cut(s) 134, 140, 206
CviJI RGCY 4 cut(s) 89, 96, 145, 161
CviKI_1 RGCY 4 cut(s) 89, 96, 145, 161
DpnI GATC 2 cut(s) 191, 279
DpnII GATC 2 cut(s) 189, 277
EaeI YGGCCR 1 cut(s) 94
Eam1104I CTCTTC 1 cut(s) 278
EarI CTCTTC 1 cut(s) 278
Eco130I CCWWGG 1 cut(s) 104
Eco31I GGTCTC 1 cut(s) 215
EcoRII CCWGG 1 cut(s) 213
EcoT14I CCWWGG 1 cut(s) 104
EcoT22I ATGCAT 1 cut(s) 51
ErhI CCWWGG 1 cut(s) 104
FaeI CATG 3 cut(s) 137, 143, 209
FaiI YATR 8 cut(s) 51, 53, 135, 141, 169, 188, 207, 230
FatI CATG 3 cut(s) 133, 139, 205
FauNDI CATATG 1 cut(s) 51
FbaI TGATCA 1 cut(s) 189
FokI GGATG 1 cut(s) 230
HaeIII GGCC 2 cut(s) 96, 161
HapII CCGG 1 cut(s) 158
Hin1II CATG 3 cut(s) 137, 143, 209
HinfI GANTC 1 cut(s) 202
HpaII CCGG 1 cut(s) 158
Hpy188I TCNGA 2 cut(s) 264, 282
Hpy188III TCNNGA 3 cut(s) 67, 126, 251
HpyAV CCTTC 1 cut(s) 73
HpyCH4III ACNGT 1 cut(s) 239
HpyCH4V TGCA 5 cut(s) 49, 133, 137, 151, 221
HpyF10VI GCNNNNNNNGC 2 cut(s) 46, 116
Hsp92II CATG 3 cut(s) 137, 143, 209
Ksp22I TGATCA 1 cut(s) 189
Kzo9I GATC 2 cut(s) 189, 277
LpnPI CCDG 4 cut(s) 65, 171, 200, 227
LweI GCATC 2 cut(s) 36, 208
MaeIII GTNAC 2 cut(s) 22, 239
MalI GATC 2 cut(s) 191, 279
MboI GATC 2 cut(s) 189, 277
MflI RGATCY 1 cut(s) 277
MlsI TGGCCA 1 cut(s) 96
MluNI TGGCCA 1 cut(s) 96
MmeI TCCRAC 1 cut(s) 155
MnlI CCTC 1 cut(s) 109
Mox20I TGGCCA 1 cut(s) 96
Mph1103I ATGCAT 1 cut(s) 51
MroXI GAANNNNTTC 1 cut(s) 62
MscI TGGCCA 1 cut(s) 96
MslI CAYNNNNRTG 1 cut(s) 138
Msp20I TGGCCA 1 cut(s) 96
MspI CCGG 1 cut(s) 158
MspR9I CCNGG 2 cut(s) 158, 215
MvaI CCWGG 1 cut(s) 215
MwoI GCNNNNNNNGC 2 cut(s) 46, 116
NciI CCSGG 1 cut(s) 158
NdeI CATATG 1 cut(s) 51
NdeII GATC 2 cut(s) 189, 277
NlaIII CATG 3 cut(s) 137, 143, 209
NlaIV GGNNCC 1 cut(s) 279
NsiI ATGCAT 1 cut(s) 51
NspI RCATGY 1 cut(s) 137
PaeI GCATGC 1 cut(s) 137
PdmI GAANNNNTTC 1 cut(s) 62
PfeI GAWTC 1 cut(s) 202
PfoI TCCNGGA 1 cut(s) 213
Psp6I CCWGG 1 cut(s) 213
PspGI CCWGG 1 cut(s) 213
PspN4I GGNNCC 1 cut(s) 279
PsuI RGATCY 1 cut(s) 277
RseI CAYNNNNRTG 1 cut(s) 138
Sau3AI GATC 2 cut(s) 189, 277
ScrFI CCNGG 2 cut(s) 158, 215
SetI ASST 3 cut(s) 84, 147, 273
SfaNI GCATC 2 cut(s) 36, 208
SmiMI CAYNNNNRTG 1 cut(s) 138
SphI GCATGC 1 cut(s) 137
StyD4I CCNGG 2 cut(s) 156, 213
StyI CCWWGG 1 cut(s) 104
TaaI ACNGT 1 cut(s) 239
TfiI GAWTC 1 cut(s) 202
TspDTI ATGAA 1 cut(s) 245
XceI RCATGY 1 cut(s) 137
XmnI GAANNNNTTC 1 cut(s) 62
Zsp2I ATGCAT 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.