Rh5AG225900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0029279
Physical Location & Seq
Reverse (-)
27031461 .. 27033414
1954 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG225900.1

Sequence Viewer

Length: 876 bp
ATGTGGAGGCGGACAGAGTTTCCGGCGGCGGCGGATTCAGGCTTTCTCCGGTGGAGGGGGGAGGTTGAGGTCGAGATCGAGCGGTCCTTTCTTGGCGGGGGAGTGAGCGAAATCCACGACGGTGGAGGAGTCCGAGTCGCTGAGGCCGGAGAGGGAACCGCCGGACGGAGAGGCGGCTGCCTTGACGCTTTTGTCCAGTTTGCCCATTTCGCGGGCGGCGAGGGGGCGATGGAGACCCGTCGTCGTCGTCGTCACCACGTTGTCGGTCCTGGCGAACGCGTCGAAGAAAAAGATGGGTCGCCCGAGGGGGAGCGCGTGGTAGGCACTCGCGGCGGTGAAGTAGCCCGCGTTGGCCCCCGCGAAACGGGGCGCTGCGAGGGTGAGGTCCAGGGCCGGAGGGGCCTGCGCCGGCGGGGACGAGGAGTCCACGGTGCTGGTCTGGCTGGGGCTGCGCGCCTGAGCCGCGGGCGGGGAATGATGGTTCTGTCTGCCGCCGCTGTTATTGTTGCCGCGGTTGTTTGTGTTCGCGTGAAGCTCGGCCGGCGTTGGGAAGTTGGTCTTAGCCTTAGCTCCACGGTACTCCCGCGCCGCCTTGTCGTACGCACGCGCCGCCTCCTCAGCCGTATCGAACGTGCCGAGCCAGACCCTCGTCTTCTTGCCAGGATCCCTGATCTCGGCAGCGTAGCGACCCCAAGGCCTCTTCCTGACGCCCCGATAACGGATCTCGAGCGAGTTCGGCTTCGGGTTCTGGGTCCCGGAGCCGGATTTTGCTGCCGTTTTCGGAGCCATGAGGTTGGTTTTTGTTTGATAGTGATTGTAGACGCAATAGAGAGAGAGGGAGAGAGAGAAGGAGAAAGAGCCTGTGAGGCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

291

Amino Acids

31.63

Weight (kDa)

10.72

Isoelectric Point (pI)

53.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029279)

Species Orthologous Gene IDs
pyrus_communis pycom05g19060
rosa_samantha Rh5AG225900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 191
AccBSI CCGCTC 1 cut(s) 82
AccI GTMKAC 1 cut(s) 819
AclWI GGATC 3 cut(s) 658, 671, 729
AcoI YGGCCR 1 cut(s) 538
AcyI GRCGYC 1 cut(s) 708
AfaI GTAC 2 cut(s) 579, 600
AfiI CCNNNNNNNGG 9 cut(s) 55, 165, 211, 364, 469, 547, 674, 718, 761
AflIII ACRYGT 1 cut(s) 277
AhdI GACNNNNNGTC 2 cut(s) 240, 422
AjnI CCWGG 3 cut(s) 268, 387, 659
AleI CACNNNNGTG 1 cut(s) 120
AloI GAACNNNNNNTCC 2 cut(s) 465, 497
AluBI AGCT 2 cut(s) 535, 570
AluI AGCT 2 cut(s) 535, 570
Alw26I GTCTC 1 cut(s) 227
AlwI GGATC 3 cut(s) 658, 671, 729
Ama87I CYCGRG 2 cut(s) 302, 725
AoxI GGCC 6 cut(s) 144, 352, 391, 400, 538, 695
ApeKI GCWGC 5 cut(s) 177, 372, 449, 678, 771
AspLEI GCGC 7 cut(s) 315, 372, 408, 454, 456, 588, 609
AspS9I GGNCC 7 cut(s) 84, 266, 353, 385, 391, 400, 752
AsuC2I CCSGG 1 cut(s) 756
AsuHPI GGTGA 3 cut(s) 245, 347, 392
AvaI CYCGRG 2 cut(s) 302, 725
AvaII GGWCC 4 cut(s) 84, 266, 385, 752
BamHI GGATCC 1 cut(s) 663
BbsI GAAGAC 1 cut(s) 644
BbvCI CCTCAGC 2 cut(s) 141, 617
BbvI GCAGC 5 cut(s) 164, 359, 436, 690, 758
BccI CCATC 3 cut(s) 223, 287, 472
BceAI ACGGC 2 cut(s) 606, 759
BciT130I CCWGG 3 cut(s) 270, 389, 661
BcnI CCSGG 1 cut(s) 756
BcoDI GTCTC 1 cut(s) 227
BfoI RGCGCY 1 cut(s) 373
BglI GCCNNNNNGGC 2 cut(s) 399, 866
Bme1390I CCNGG 4 cut(s) 270, 389, 661, 756
Bme18I GGWCC 4 cut(s) 84, 266, 385, 752
BmeRI GACNNNNNGTC 2 cut(s) 240, 422
BmeT110I CYCGRG 2 cut(s) 302, 725
BmgT120I GGNCC 7 cut(s) 84, 266, 353, 385, 391, 400, 752
BmiI GGNNCC 8 cut(s) 157, 355, 401, 665, 753, 754, 760, 785
BmrFI CCNGG 4 cut(s) 270, 389, 661, 756
BoxI GACNNNNGTC 1 cut(s) 648
BpiI GAAGAC 1 cut(s) 644
Bpu10I CCTNAGC 4 cut(s) 141, 458, 566, 617
BpuMI CCSGG 1 cut(s) 756
BsaHI GRCGYC 1 cut(s) 708
BsaI GGTCTC 1 cut(s) 227
BsaJI CCNNGG 7 cut(s) 303, 388, 427, 463, 510, 573, 692
BsaWI WCCGGW 1 cut(s) 48
BsaXI ACNNNNNCTCC 2 cut(s) 120, 150
Bsc4I CCNNNNNNNGG 9 cut(s) 55, 165, 211, 364, 469, 547, 674, 718, 761
Bse118I RCCGGY 2 cut(s) 408, 540
Bse1I ACTGG 1 cut(s) 196
BseBI CCWGG 3 cut(s) 270, 389, 661
BseDI CCNNGG 7 cut(s) 303, 388, 427, 463, 510, 573, 692
BseLI CCNNNNNNNGG 9 cut(s) 55, 165, 211, 364, 469, 547, 674, 718, 761
BseMII CTCAG 3 cut(s) 132, 449, 631
BseNI ACTGG 1 cut(s) 196
BsePI GCGCGC 1 cut(s) 452
BseRI GAGGAG 3 cut(s) 141, 435, 605
BseX3I CGGCCG 1 cut(s) 538
BseXI GCAGC 5 cut(s) 164, 359, 436, 690, 758
BseYI CCCAGC 1 cut(s) 443
Bsh1285I CGRYCG 1 cut(s) 541
BshFI GGCC 6 cut(s) 146, 354, 393, 402, 540, 697
BsiEI CGRYCG 1 cut(s) 541
BsiHKCI CYCGRG 2 cut(s) 302, 725
BsiSI CCGG 9 cut(s) 23, 49, 147, 162, 394, 409, 541, 756, 762
BsiWI CGTACG 1 cut(s) 598
BslFI GGGAC 2 cut(s) 429, 738
BslI CCNNNNNNNGG 9 cut(s) 55, 165, 211, 364, 469, 547, 674, 718, 761
BsmAI GTCTC 1 cut(s) 227
BsmFI GGGAC 2 cut(s) 429, 738
BsnI GGCC 6 cut(s) 146, 354, 393, 402, 540, 697
Bso31I GGTCTC 1 cut(s) 227
BsoBI CYCGRG 2 cut(s) 302, 725
Bsp143I GATC 4 cut(s) 75, 663, 670, 721
BspANI GGCC 6 cut(s) 146, 354, 393, 402, 540, 697
BspCNI CTCAG 3 cut(s) 133, 450, 630
BspLI GGNNCC 8 cut(s) 157, 355, 401, 665, 753, 754, 760, 785
BspPI GGATC 3 cut(s) 658, 671, 729
BspTNI GGTCTC 1 cut(s) 227
BsrBI CCGCTC 1 cut(s) 82
BsrFI RCCGGY 2 cut(s) 408, 540
BsrI ACTGG 1 cut(s) 196
BssAI RCCGGY 2 cut(s) 408, 540
BssECI CCNNGG 7 cut(s) 303, 388, 427, 463, 510, 573, 692
BssHII GCGCGC 1 cut(s) 452
BssMI GATC 4 cut(s) 75, 663, 670, 721
BssNI GRCGYC 1 cut(s) 708
BssT1I CCWWGG 1 cut(s) 692
Bst2UI CCWGG 3 cut(s) 270, 389, 661
Bst4CI ACNGT 3 cut(s) 122, 431, 577
Bst6I CTCTTC 1 cut(s) 705
BstACI GRCGYC 1 cut(s) 708
BstC8I GCNNGC 8 cut(s) 214, 346, 404, 410, 454, 467, 542, 605
BstDEI CTNAG 5 cut(s) 141, 458, 560, 566, 617
BstDSI CCRYGG 4 cut(s) 427, 463, 510, 573
BstH2I RGCGCY 1 cut(s) 373
BstHHI GCGC 7 cut(s) 315, 372, 408, 454, 456, 588, 609
BstKTI GATC 4 cut(s) 78, 666, 673, 724
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 4 cut(s) 75, 663, 670, 721
BstMCI CGRYCG 1 cut(s) 541
BstNI CCWGG 3 cut(s) 270, 389, 661
BstPAI GACNNNNGTC 1 cut(s) 648
BstSCI CCNGG 4 cut(s) 268, 387, 659, 754
BstV1I GCAGC 5 cut(s) 164, 359, 436, 690, 758
BstV2I GAAGAC 1 cut(s) 644
BstX2I RGATCY 2 cut(s) 663, 721
BstXI CCANNNNNNTGG 3 cut(s) 122, 434, 794
BstYI RGATCY 2 cut(s) 663, 721
BstZI CGGCCG 1 cut(s) 538
BsuRI GGCC 6 cut(s) 146, 354, 393, 402, 540, 697
BtgI CCRYGG 4 cut(s) 427, 463, 510, 573
BtgZI GCGATG 1 cut(s) 242
Cac8I GCNNGC 8 cut(s) 214, 346, 404, 410, 454, 467, 542, 605
CfoI GCGC 7 cut(s) 315, 372, 408, 454, 456, 588, 609
Cfr10I RCCGGY 2 cut(s) 408, 540
Cfr13I GGNCC 7 cut(s) 84, 266, 353, 385, 391, 400, 752
Cfr42I CCGCGG 2 cut(s) 466, 513
CseI GACGC 4 cut(s) 194, 268, 716, 830
Csp6I GTAC 2 cut(s) 578, 599
CviAII CATG 1 cut(s) 788
CviQI GTAC 2 cut(s) 578, 599
DdeI CTNAG 5 cut(s) 141, 458, 560, 566, 617
DpnI GATC 4 cut(s) 77, 665, 672, 723
DpnII GATC 4 cut(s) 75, 663, 670, 721
DrdI GACNNNNNNGTC 1 cut(s) 191
DriI GACNNNNNGTC 2 cut(s) 240, 422
DseDI GACNNNNNNGTC 1 cut(s) 191
EaeI YGGCCR 1 cut(s) 538
EagI CGGCCG 1 cut(s) 538
Eam1104I CTCTTC 1 cut(s) 705
Eam1105I GACNNNNNGTC 2 cut(s) 240, 422
EarI CTCTTC 1 cut(s) 705
EciI GGCGGA 2 cut(s) 25, 47
EclXI CGGCCG 1 cut(s) 538
Eco130I CCWWGG 1 cut(s) 692
Eco147I AGGCCT 1 cut(s) 697
Eco31I GGTCTC 1 cut(s) 227
Eco47I GGWCC 4 cut(s) 84, 266, 385, 752
Eco52I CGGCCG 1 cut(s) 538
Eco88I CYCGRG 2 cut(s) 302, 725
EcoO109I RGGNCCY 2 cut(s) 400, 752
EcoRII CCWGG 3 cut(s) 268, 387, 659
EcoT14I CCWWGG 1 cut(s) 692
ErhI CCWWGG 1 cut(s) 692
FaeI CATG 1 cut(s) 791
FaiI YATR 1 cut(s) 789
FalI AAGNNNNNCTT 2 cut(s) 543, 575
FaqI GGGAC 2 cut(s) 429, 738
FatI CATG 1 cut(s) 787
FauI CCCGC 8 cut(s) 89, 205, 353, 365, 405, 458, 462, 591
FblI GTMKAC 1 cut(s) 819
GlaI GCGC 7 cut(s) 314, 371, 407, 453, 455, 587, 608
GsaI CCCAGC 1 cut(s) 447
HaeII RGCGCY 1 cut(s) 373
HaeIII GGCC 6 cut(s) 146, 354, 393, 402, 540, 697
HapII CCGG 9 cut(s) 23, 49, 147, 162, 394, 409, 541, 756, 762
HgaI GACGC 4 cut(s) 194, 268, 716, 830
HhaI GCGC 7 cut(s) 315, 372, 408, 454, 456, 588, 609
Hin1I GRCGYC 1 cut(s) 708
Hin1II CATG 1 cut(s) 791
Hin6I GCGC 7 cut(s) 313, 370, 406, 452, 454, 586, 607
HinP1I GCGC 7 cut(s) 313, 370, 406, 452, 454, 586, 607
HinfI GANTC 4 cut(s) 35, 129, 135, 423
HpaII CCGG 9 cut(s) 23, 49, 147, 162, 394, 409, 541, 756, 762
HphI GGTGA 3 cut(s) 245, 347, 392
Hpy166II GTNNAC 2 cut(s) 427, 820
Hpy188I TCNGA 2 cut(s) 134, 783
Hpy188III TCNNGA 3 cut(s) 73, 704, 725
Hpy8I GTNNAC 2 cut(s) 427, 820
Hpy99I CGWCG 6 cut(s) 122, 243, 246, 249, 252, 284
HpyAV CCTTC 1 cut(s) 842
HpyCH4III ACNGT 3 cut(s) 122, 431, 577
HpyCH4IV ACGT 2 cut(s) 258, 631
HpyF3I CTNAG 5 cut(s) 141, 458, 560, 566, 617
HpySE526I ACGT 2 cut(s) 258, 631
Hsp92I GRCGYC 1 cut(s) 708
Hsp92II CATG 1 cut(s) 791
HspAI GCGC 7 cut(s) 313, 370, 406, 452, 454, 586, 607
KflI GGGWCCC 1 cut(s) 752
KroI GCCGGC 2 cut(s) 408, 540
KroNI GCCGGC 2 cut(s) 410, 542
KspI CCGCGG 2 cut(s) 466, 513
Kzo9I GATC 4 cut(s) 75, 663, 670, 721
LmnI GCTCC 4 cut(s) 310, 575, 758, 783
Lsp1109I GCAGC 5 cut(s) 164, 359, 436, 690, 758
MaeII ACGT 2 cut(s) 258, 631
MaeIII GTNAC 1 cut(s) 251
MalI GATC 4 cut(s) 77, 665, 672, 723
MbiI CCGCTC 1 cut(s) 82
MboI GATC 4 cut(s) 75, 663, 670, 721
MboII GAAGA 3 cut(s) 296, 644, 692
MflI RGATCY 2 cut(s) 663, 721
MluI ACGCGT 1 cut(s) 277
MlyI GAGTC 3 cut(s) 138, 144, 432
MreI CGCCGGCG 1 cut(s) 408
MroNI GCCGGC 2 cut(s) 408, 540
MslI CAYNNNNRTG 1 cut(s) 120
MspA1I CMGCKG 3 cut(s) 465, 497, 512
MspI CCGG 9 cut(s) 23, 49, 147, 162, 394, 409, 541, 756, 762
MspR9I CCNGG 4 cut(s) 270, 389, 661, 756
MvaI CCWGG 3 cut(s) 270, 389, 661
NaeI GCCGGC 2 cut(s) 410, 542
NciI CCSGG 1 cut(s) 756
NdeII GATC 4 cut(s) 75, 663, 670, 721
NgoMIV GCCGGC 2 cut(s) 408, 540
NlaIII CATG 1 cut(s) 791
NlaIV GGNNCC 8 cut(s) 157, 355, 401, 665, 753, 754, 760, 785
NmeAIII GCCGAG 3 cut(s) 516, 654, 661
NmuCI GTSAC 1 cut(s) 251
OliI CACNNNNGTG 1 cut(s) 120
PaeR7I CTCGAG 1 cut(s) 725
PauI GCGCGC 1 cut(s) 452
PceI AGGCCT 1 cut(s) 697
PcsI WCGNNNNNNNCGW 4 cut(s) 216, 247, 270, 633
PdiI GCCGGC 2 cut(s) 410, 542
PfeI GAWTC 1 cut(s) 35
Pfl23II CGTACG 1 cut(s) 598
PfoI TCCNGGA 1 cut(s) 754
PleI GAGTC 3 cut(s) 137, 143, 431
PpsI GAGTC 3 cut(s) 137, 143, 431
PpuMI RGGWCCY 1 cut(s) 752
PshAI GACNNNNGTC 1 cut(s) 648
Psp5II RGGWCCY 1 cut(s) 752
Psp6I CCWGG 3 cut(s) 268, 387, 659
PspFI CCCAGC 1 cut(s) 443
PspGI CCWGG 3 cut(s) 268, 387, 659
PspLI CGTACG 1 cut(s) 598
PspN4I GGNNCC 8 cut(s) 157, 355, 401, 665, 753, 754, 760, 785
PspPI GGNCC 7 cut(s) 84, 266, 353, 385, 391, 400, 752
PspPPI RGGWCCY 1 cut(s) 752
PsuI RGATCY 2 cut(s) 663, 721
PteI GCGCGC 1 cut(s) 452
RsaI GTAC 2 cut(s) 579, 600
RsaNI GTAC 2 cut(s) 578, 599
RseI CAYNNNNRTG 1 cut(s) 120
SacII CCGCGG 2 cut(s) 466, 513
Sau3AI GATC 4 cut(s) 75, 663, 670, 721
Sau96I GGNCC 7 cut(s) 84, 266, 353, 385, 391, 400, 752
SchI GAGTC 3 cut(s) 138, 144, 432
ScrFI CCNGG 4 cut(s) 270, 389, 661, 756
SetI ASST 8 cut(s) 66, 72, 261, 387, 537, 572, 634, 795
SfiI GGCCNNNNNGGCC 1 cut(s) 399
Sfr274I CTCGAG 1 cut(s) 725
Sfr303I CCGCGG 2 cut(s) 466, 513
SgrAI CRCCGGYG 1 cut(s) 408
SgrBI CCGCGG 2 cut(s) 466, 513
SinI GGWCC 4 cut(s) 84, 266, 385, 752
SlaI CTCGAG 1 cut(s) 725
SmiMI CAYNNNNRTG 1 cut(s) 120
SmlI CTYRAG 1 cut(s) 725
SmoI CTYRAG 1 cut(s) 725
SseBI AGGCCT 1 cut(s) 697
StuI AGGCCT 1 cut(s) 697
StyD4I CCNGG 4 cut(s) 268, 387, 659, 754
StyI CCWWGG 1 cut(s) 692
TaaI ACNGT 3 cut(s) 122, 431, 577
TaiI ACGT 2 cut(s) 261, 634
TaqI TCGA 5 cut(s) 72, 78, 282, 627, 726
TaqII GACCGA 1 cut(s) 254
TfiI GAWTC 1 cut(s) 35
TseFI GTSAC 1 cut(s) 251
TseI GCWGC 5 cut(s) 177, 372, 449, 678, 771
Tsp45I GTSAC 1 cut(s) 251
TspGWI ACGGA 2 cut(s) 181, 734
VpaK11BI GGWCC 4 cut(s) 84, 266, 385, 752
XhoI CTCGAG 1 cut(s) 725
XmiI GTMKAC 1 cut(s) 819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.