Rh5AG343400

Cell division cycle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0025532
Physical Location & Seq
Forward (+)
56217720 .. 56221701
3982 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG343400.1

Sequence Viewer

Length: 465 bp
ATGGAATTCCGCTGCTTTGTAAGGAATCAGAATTTAGTTGGAATTTCCCAACGCGAGGCCACAACCTTCTACCCTTCTCTGCTTCAAAGCAAAGAGCATCTGGGAGTGTTGATCGAAGAGTTCTTTATGGAAAATTTAAGGTCAAGATTCGAGTCAGAAAATTATACCTTTGATGTATATGTGACAAAGGACGAGCGAGTTAAGGTTATGGATTTCAACCCTTGGGGTGCATTTACACTGCCTCTGATGTTTAGATGGGAGGAACTGGAACAGAGTGATAATGAACAAGGGGATGGTGTGGACTTCAGAATTGTGGAGAGCCAGTGTGGTGTTAGGCCAGGTTTGAAGACAGCAGTGCCCTACGATTATGTGGACACTAGCGCTGGGAGTGGCTGGGACCAGTTTTTCAGGAACGCCGATAAAGAGTTACAACGGCAGAGTAGGTCTCCTGAAGCAGGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
D123

Protein Analysis

154

Amino Acids

17.95

Weight (kDa)

4.67

Isoelectric Point (pI)

57.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
D123 PF07065 1 - 127 1.5e-40 D123
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025532)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002311.1_g000025
rosa_roxburghii Rroxscaffold_1G00061080
rosa_samantha Rh5AG343400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 446
Acc36I ACCTGC 1 cut(s) 446
AccII CGCG 1 cut(s) 54
AciI CCGC 1 cut(s) 10
AcsI RAATTY 4 cut(s) 5, 31, 42, 133
AcuI CTGAAG 1 cut(s) 289
AfeI AGCGCT 1 cut(s) 382
AfiI CCNNNNNNNGG 2 cut(s) 55, 455
AgsI TTSAA 3 cut(s) 86, 217, 346
AjnI CCWGG 1 cut(s) 337
Alw26I GTCTC 1 cut(s) 450
Aor51HI AGCGCT 1 cut(s) 382
AoxI GGCC 2 cut(s) 57, 335
ApeKI GCWGC 1 cut(s) 12
ApoI RAATTY 4 cut(s) 5, 31, 42, 133
AspLEI GCGC 1 cut(s) 383
AspS9I GGNCC 1 cut(s) 397
AvaII GGWCC 1 cut(s) 397
BaeGI GKGCMC 1 cut(s) 360
BbsI GAAGAC 1 cut(s) 353
BccI CCATC 2 cut(s) 249, 287
BceAI ACGGC 1 cut(s) 449
BciT130I CCWGG 1 cut(s) 339
BcoDI GTCTC 1 cut(s) 450
BfaI CTAG 1 cut(s) 378
BfoI RGCGCY 1 cut(s) 384
BfuAI ACCTGC 1 cut(s) 446
BisI GCNGC 1 cut(s) 13
BlsI GCNGC 1 cut(s) 14
Bme1390I CCNGG 1 cut(s) 339
Bme18I GGWCC 1 cut(s) 397
BmgT120I GGNCC 1 cut(s) 397
BmiI GGNNCC 1 cut(s) 398
BmrFI CCNGG 1 cut(s) 339
BmsI GCATC 1 cut(s) 106
BpiI GAAGAC 1 cut(s) 353
BplI GAGNNNNNCTC 2 cut(s) 430, 462
BsaI GGTCTC 1 cut(s) 450
BsaJI CCNNGG 1 cut(s) 221
BsaXI ACNNNNNCTCC 2 cut(s) 308, 338
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 455
Bse1I ACTGG 3 cut(s) 270, 322, 400
BseBI CCWGG 1 cut(s) 339
BseDI CCNNGG 1 cut(s) 221
BseGI GGATG 1 cut(s) 298
BseLI CCNNNNNNNGG 2 cut(s) 55, 455
BseNI ACTGG 3 cut(s) 270, 322, 400
BseSI GKGCMC 1 cut(s) 360
BseYI CCCAGC 2 cut(s) 383, 393
Bsh1236I CGCG 1 cut(s) 54
BshFI GGCC 2 cut(s) 59, 337
BslFI GGGAC 1 cut(s) 410
BslI CCNNNNNNNGG 2 cut(s) 55, 455
BsmAI GTCTC 1 cut(s) 450
BsmFI GGGAC 1 cut(s) 410
BsnI GGCC 2 cut(s) 59, 337
Bso31I GGTCTC 1 cut(s) 450
Bsp1286I GDGCHC 1 cut(s) 360
Bsp143I GATC 1 cut(s) 111
BspACI CCGC 1 cut(s) 10
BspANI GGCC 2 cut(s) 59, 337
BspFNI CGCG 1 cut(s) 54
BspLI GGNNCC 1 cut(s) 398
BspMI ACCTGC 1 cut(s) 446
BspTNI GGTCTC 1 cut(s) 450
BsrI ACTGG 3 cut(s) 270, 322, 400
BssECI CCNNGG 1 cut(s) 221
BssMI GATC 1 cut(s) 111
BssT1I CCWWGG 1 cut(s) 221
Bst2UI CCWGG 1 cut(s) 339
Bst6I CTCTTC 1 cut(s) 111
BstENI CCTNNNNNAGG 1 cut(s) 453
BstF5I GGATG 1 cut(s) 298
BstFNI CGCG 1 cut(s) 54
BstH2I RGCGCY 1 cut(s) 384
BstHHI GCGC 1 cut(s) 383
BstKTI GATC 1 cut(s) 114
BstMAI GTCTC 1 cut(s) 450
BstMBI GATC 1 cut(s) 111
BstNI CCWGG 1 cut(s) 339
BstSCI CCNGG 1 cut(s) 337
BstSLI GKGCMC 1 cut(s) 360
BstUI CGCG 1 cut(s) 54
BstV2I GAAGAC 1 cut(s) 353
BsuRI GGCC 2 cut(s) 59, 337
BtsCI GGATG 1 cut(s) 298
BtsI GCAGTG 2 cut(s) 236, 360
BtsIMutI CAGTG 3 cut(s) 236, 329, 360
BveI ACCTGC 1 cut(s) 446
CfoI GCGC 1 cut(s) 383
Cfr13I GGNCC 1 cut(s) 397
CviJI RGCY 4 cut(s) 59, 321, 337, 393
CviKI_1 RGCY 4 cut(s) 59, 321, 337, 393
DpnI GATC 1 cut(s) 113
DpnII GATC 1 cut(s) 111
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 221
Eco31I GGTCTC 1 cut(s) 450
Eco47I GGWCC 1 cut(s) 397
Eco47III AGCGCT 1 cut(s) 382
Eco57I CTGAAG 1 cut(s) 289
EcoNI CCTNNNNNAGG 1 cut(s) 453
EcoRI GAATTC 1 cut(s) 5
EcoRII CCWGG 1 cut(s) 337
EcoT14I CCWWGG 1 cut(s) 221
ErhI CCWWGG 1 cut(s) 221
FaiI YATR 6 cut(s) 128, 165, 178, 180, 209, 369
FaqI GGGAC 1 cut(s) 410
Fnu4HI GCNGC 1 cut(s) 13
FokI GGATG 1 cut(s) 305
Fsp4HI GCNGC 1 cut(s) 13
FspBI CTAG 1 cut(s) 378
GlaI GCGC 1 cut(s) 382
GluI GCNGC 1 cut(s) 13
GsaI CCCAGC 2 cut(s) 387, 397
HaeII RGCGCY 1 cut(s) 384
HaeIII GGCC 2 cut(s) 59, 337
HhaI GCGC 1 cut(s) 383
Hin6I GCGC 1 cut(s) 381
HinP1I GCGC 1 cut(s) 381
HinfI GANTC 3 cut(s) 25, 147, 152
Hpy166II GTNNAC 2 cut(s) 301, 373
Hpy188I TCNGA 4 cut(s) 30, 157, 246, 308
Hpy188III TCNNGA 3 cut(s) 144, 409, 449
Hpy8I GTNNAC 2 cut(s) 301, 373
HpyAV CCTTC 2 cut(s) 76, 84
HpyCH4V TGCA 1 cut(s) 230
HspAI GCGC 1 cut(s) 381
Kzo9I GATC 1 cut(s) 111
LweI GCATC 1 cut(s) 106
MaeI CTAG 1 cut(s) 378
MaeIII GTNAC 2 cut(s) 181, 426
MalI GATC 1 cut(s) 113
MboI GATC 1 cut(s) 111
MboII GAAGA 2 cut(s) 128, 358
MhlI GDGCHC 1 cut(s) 360
MluCI AATT 6 cut(s) 5, 31, 42, 133, 160, 309
MlyI GAGTC 1 cut(s) 161
MmeI TCCRAC 1 cut(s) 19
MnlI CCTC 3 cut(s) 49, 252, 253
MseI TTAA 3 cut(s) 137, 201, 463
MspA1I CMGCKG 1 cut(s) 12
MspR9I CCNGG 1 cut(s) 339
MvaI CCWGG 1 cut(s) 339
MvnI CGCG 1 cut(s) 54
NdeII GATC 1 cut(s) 111
NlaIV GGNNCC 1 cut(s) 398
NmuCI GTSAC 1 cut(s) 181
PaqCI CACCTGC 1 cut(s) 446
PfeI GAWTC 2 cut(s) 25, 147
PkrI GCNGC 1 cut(s) 14
PleI GAGTC 1 cut(s) 160
PpsI GAGTC 1 cut(s) 160
Psp6I CCWGG 1 cut(s) 337
PspFI CCCAGC 2 cut(s) 383, 393
PspGI CCWGG 1 cut(s) 337
PspN4I GGNNCC 1 cut(s) 398
PspPI GGNCC 1 cut(s) 397
SaqAI TTAA 3 cut(s) 137, 201, 463
SatI GCNGC 1 cut(s) 13
Sau3AI GATC 1 cut(s) 111
Sau96I GGNCC 1 cut(s) 397
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 1 cut(s) 339
SduI GDGCHC 1 cut(s) 360
SetI ASST 7 cut(s) 68, 143, 170, 207, 343, 446, 460
SfaNI GCATC 1 cut(s) 106
SinI GGWCC 1 cut(s) 397
Sse9I AATT 6 cut(s) 5, 31, 42, 133, 160, 309
SsiI CCGC 1 cut(s) 10
SspMI CTAG 1 cut(s) 378
StyD4I CCNGG 1 cut(s) 337
StyI CCWWGG 1 cut(s) 221
TaqI TCGA 2 cut(s) 114, 150
TasI AATT 6 cut(s) 5, 31, 42, 133, 160, 309
TfiI GAWTC 2 cut(s) 25, 147
Tru1I TTAA 3 cut(s) 137, 201, 463
Tru9I TTAA 3 cut(s) 137, 201, 463
TscAI CASTG 3 cut(s) 243, 329, 360
TseFI GTSAC 1 cut(s) 181
TseI GCWGC 1 cut(s) 12
Tsp45I GTSAC 1 cut(s) 181
TspDTI ATGAA 1 cut(s) 297
TspRI CASTG 3 cut(s) 243, 329, 360
VpaK11BI GGWCC 1 cut(s) 397
XagI CCTNNNNNAGG 1 cut(s) 453
XapI RAATTY 4 cut(s) 5, 31, 42, 133
XspI CTAG 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.