Rh5AG436200

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0016128
Physical Location & Seq
Forward (+)
74946307 .. 74948812
2506 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG436200.1

Sequence Viewer

Length: 1527 bp
ATGGCTGCACCTCTCACTTCCAATTCATTCGTTCTAACTTCCTCACCCAATTCAAGAGCAACAACCACTCTCAGGAACCCCCGAAGGTTCACCGTCTTTGCCAAGAAGGCTGGACCCTTCTCTACATTTCGGCTTGGCAAGTCATCAGGGGATGTTGGTTCATCGTCCTCCGGCGAAGGCGAAGCCGATGACTCCAATTCAAGTCCATTCAAGTTTAATTTCGGGAAATTACCCGATGTCAAGTCTTTGATCCCCGTTGCGAGTAGGCCTTCTTTTTCATTTGGGACCTCCAGAAGCAAGGACCCTGGGACTGTGTTTGTTGCTGGTGCAACAGGGCAGGCTGGGGTCCGAATAGCACAGACCCTTCTGCGTGAAGGTTTTAGTGTGAGGGCTGGTGTGCCTGAACTGAGTGATGCCCAAGAATTGGCTCGTGTTGCTTCTAAATACAAGATCCTATCAAATGAGGAATCAAAACGCCTCAATGCTGTGGGGTCTACCTTCGAAGATGCTGAGACTATCGCCAAAGCCATCGGAAATGCCAGCAAAGTTGTTGTCACAATTGGCCCCTTCGAGAATGGTCCAACGACTGAAGTCACACCATCAGACGCCTTACAAGTAATCCAAGCTGCTCAGCTCGCCGGTGTCGGTCATGTGGCCATCGTCTATGATGGTAACATAGCAGGTGCATCGACTTACAATGTAGTAGATGGCATATCTTCATTCTTTAGCAACATCTTCTCGCGATCTCAGCCGTTGAGCATCACTGAATTGTTGCAAAACGTCATTCAAACAGACGTTGGCTACACTTTCATAAAGACAAGTTTGACGGATGATTTTTCGCCCGAGAGCTCATATAATGTTGTTGTGTCAGCTGAAGGAAACGGAGGTGCTAACGACTACAAAGTAGCCAAGTCTCAGATTGCTTCCCTGGTGGCAGATGTTTTCTCAAACACTTCTGTGGCAGAAAATAAGGTTGTGGAGGTATATACTGATCCATCAGCACCGTTGAGGCCTGTAGATCAACTTTTCAGTACAATCCCTGAGGATGGAAGAAGGAAAGCTTATGCAGAAGCGATTGCAAAGGCAAAGGCAGAAGAAGAGGCAATAATAGCTGCTGAGAGAGCTCGCAAAGCAGCCGAGGCAGCAAAGTCACTTGAAGAAGAAGTAAAGAAGCTATCCGAACAAGAAGTTCAAGTAAAGGCTGAGGCTGCAGGGGCCTCATTTGATGGCCTCGTAAGCAAAGCAAAAGGCTTTGGCTCAGGGCTGTCTTGGGATAAGTTTAGCTCACAACTTGCAGACACAGTTTCAAAATCCATGGAGACATCAAAAGTTCAGATTGCTACTGTAAGGGGACAGGCCAAGGCTAAGACACTGCCTCCGCTGAAAGCAGTTGCTAAGAAAGTTGCAACTCCGAAACCAAAGGAGGAGGCAAAGCCTAAATCCAAACAAACAGAGGCGAAGACTGAAGTGAGGAGGGTATTTGGTGGCTTGTTCACACAAGAGACTGTGTATGTTGATGATGATTGA

Protein Analysis

508

Amino Acids

53.72

Weight (kDa)

8.34

Isoelectric Point (pI)

38.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 104 - 218 5e-06 NmrA-like family
NAD_binding_10 PF13460 109 - 317 2.4e-11 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016128)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46780
fragaria_vesca FvH4_3g36950
malus_domestica MD03G1086000.v1.1
prunus_persica Prupe.6G070200_v2.0.a1
pyrus_communis pycom03g06790 pycom11g08030
rosa_chinensis RchiOBHm_Chr5g0066371
rosa_laevigata RLG00000035855
rosa_multiflora Rmu_ssc0000249.1_g000009
rosa_roxburghii Rroxscaffold_1G00014770
rosa_samantha Rh5AG436200 Rh5BG452600 Rh5CG475000 Rh5DG467800
rosa_wichuraiana Rw5G040860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 671
Acc36I ACCTGC 1 cut(s) 671
AccB7I CCANNNNNTGG 1 cut(s) 424
AccI GTMKAC 1 cut(s) 494
AccII CGCG 1 cut(s) 742
AciI CCGC 1 cut(s) 1379
AclWI GGATC 3 cut(s) 244, 445, 986
AcoI YGGCCR 1 cut(s) 654
AcuI CTGAAG 3 cut(s) 609, 894, 1485
AcyI GRCGYC 1 cut(s) 606
AfaI GTAC 1 cut(s) 1033
AfiI CCNNNNNNNGG 3 cut(s) 72, 424, 1046
AgsI TTSAA 7 cut(s) 54, 201, 211, 788, 1157, 1193, 1308
AjnI CCWGG 2 cut(s) 304, 927
AleI CACNNNNGTG 1 cut(s) 956
AluBI AGCT 9 cut(s) 626, 634, 849, 872, 1061, 1112, 1124, 1174, 1284
AluI AGCT 9 cut(s) 626, 634, 849, 872, 1061, 1112, 1124, 1174, 1284
Alw21I GWGCWC 2 cut(s) 851, 1126
Alw26I GTCTC 4 cut(s) 506, 918, 1313, 1496
AlwI GGATC 3 cut(s) 244, 445, 986
Ama87I CYCGRG 1 cut(s) 842
AoxI GGCC 7 cut(s) 266, 562, 654, 1010, 1215, 1228, 1356
ApeKI GCWGC 6 cut(s) 5, 626, 1112, 1133, 1142, 1208
ArsI GACNNNNNNTTYG 2 cut(s) 537, 569
AspS9I GGNCC 7 cut(s) 113, 285, 301, 346, 563, 578, 1215
AsuHPI GGTGA 2 cut(s) 36, 82
AsuII TTCGAA 1 cut(s) 501
AvaI CYCGRG 1 cut(s) 842
AvaII GGWCC 5 cut(s) 113, 285, 301, 346, 578
AxyI CCTNAGG 1 cut(s) 1041
BalI TGGCCA 1 cut(s) 656
BanII GRGCYC 2 cut(s) 851, 1126
BauI CACGAG 1 cut(s) 429
BbsI GAAGAC 1 cut(s) 1466
Bbv12I GWGCWC 2 cut(s) 851, 1126
BbvCI CCTCAGC 1 cut(s) 1203
BbvI GCAGC 5 cut(s) 613, 1099, 1145, 1154, 1195
BccI CCATC 8 cut(s) 536, 607, 662, 665, 701, 1003, 1040, 1220
BceAI ACGGC 1 cut(s) 736
BcgI CGANNNNNNTGC 2 cut(s) 669, 703
BciT130I CCWGG 2 cut(s) 306, 929
BcoDI GTCTC 4 cut(s) 506, 918, 1313, 1496
BfmI CTRYAG 2 cut(s) 1014, 1209
BfuAI ACCTGC 1 cut(s) 671
BglI GCCNNNNNGGC 1 cut(s) 107
BisI GCNGC 6 cut(s) 6, 627, 1113, 1134, 1143, 1209
BlpI GCTNAGC 1 cut(s) 630
BlsI GCNGC 6 cut(s) 7, 628, 1114, 1135, 1144, 1210
Bme1390I CCNGG 2 cut(s) 306, 929
Bme18I GGWCC 5 cut(s) 113, 285, 301, 346, 578
BmeT110I CYCGRG 1 cut(s) 842
BmgT120I GGNCC 7 cut(s) 113, 285, 301, 346, 563, 578, 1215
BmiI GGNNCC 7 cut(s) 77, 115, 286, 303, 347, 565, 1216
BmrFI CCNGG 2 cut(s) 306, 929
BmsI GCATC 4 cut(s) 403, 496, 695, 768
BoxI GACNNNNGTC 1 cut(s) 590
BpiI GAAGAC 1 cut(s) 1466
BpmI CTGGAG 1 cut(s) 274
Bpu10I CCTNAGC 2 cut(s) 1203, 1258
Bpu1102I GCTNAGC 1 cut(s) 630
Bpu14I TTCGAA 1 cut(s) 501
BsaHI GRCGYC 1 cut(s) 606
BsaJI CCNNGG 6 cut(s) 304, 305, 927, 1137, 1314, 1359
BsaXI ACNNNNNCTCC 2 cut(s) 1360, 1390
Bsc4I CCNNNNNNNGG 3 cut(s) 72, 424, 1046
Bse118I RCCGGY 1 cut(s) 638
Bse21I CCTNAGG 1 cut(s) 1041
BseBI CCWGG 2 cut(s) 306, 929
BseDI CCNNGG 6 cut(s) 304, 305, 927, 1137, 1314, 1359
BseGI GGATG 3 cut(s) 157, 835, 1051
BseLI CCNNNNNNNGG 3 cut(s) 72, 424, 1046
BseRI GAGGAG 2 cut(s) 1439, 1486
BseXI GCAGC 5 cut(s) 613, 1099, 1145, 1154, 1195
BseYI CCCAGC 1 cut(s) 341
Bsh1236I CGCG 1 cut(s) 742
BshFI GGCC 7 cut(s) 268, 564, 656, 1012, 1217, 1230, 1358
BsiHKAI GWGCWC 2 cut(s) 851, 1126
BsiHKCI CYCGRG 1 cut(s) 842
BsiSI CCGG 2 cut(s) 171, 639
BslFI GGGAC 3 cut(s) 298, 322, 1365
BslI CCNNNNNNNGG 3 cut(s) 72, 424, 1046
BsmAI GTCTC 4 cut(s) 506, 918, 1313, 1496
BsmFI GGGAC 3 cut(s) 298, 322, 1365
BsnI GGCC 7 cut(s) 268, 564, 656, 1012, 1217, 1230, 1358
BsoBI CYCGRG 1 cut(s) 842
Bsp119I TTCGAA 1 cut(s) 501
Bsp1286I GDGCHC 2 cut(s) 851, 1126
Bsp143I GATC 5 cut(s) 249, 450, 743, 991, 1018
Bsp1720I GCTNAGC 1 cut(s) 630
Bsp19I CCATGG 1 cut(s) 1314
Bsp68I TCGCGA 1 cut(s) 742
BspACI CCGC 1 cut(s) 1379
BspANI GGCC 7 cut(s) 268, 564, 656, 1012, 1217, 1230, 1358
BspFNI CGCG 1 cut(s) 742
BspLI GGNNCC 7 cut(s) 77, 115, 286, 303, 347, 565, 1216
BspMAI CTGCAG 1 cut(s) 1213
BspMI ACCTGC 1 cut(s) 671
BspPI GGATC 3 cut(s) 244, 445, 986
BspT104I TTCGAA 1 cut(s) 501
BsrFI RCCGGY 1 cut(s) 638
BssAI RCCGGY 1 cut(s) 638
BssECI CCNNGG 6 cut(s) 304, 305, 927, 1137, 1314, 1359
BssMI GATC 5 cut(s) 249, 450, 743, 991, 1018
BssNI GRCGYC 1 cut(s) 606
BssSI CACGAG 1 cut(s) 429
BssT1I CCWWGG 2 cut(s) 1314, 1359
Bst2BI CACGAG 1 cut(s) 429
Bst2UI CCWGG 2 cut(s) 306, 929
Bst4CI ACNGT 6 cut(s) 94, 313, 1005, 1303, 1345, 1507
Bst6I CTCTTC 1 cut(s) 1092
BstACI GRCGYC 1 cut(s) 606
BstBI TTCGAA 1 cut(s) 501
BstC8I GCNNGC 4 cut(s) 339, 541, 636, 1126
BstDSI CCRYGG 1 cut(s) 1314
BstF5I GGATG 3 cut(s) 157, 835, 1051
BstFNI CGCG 1 cut(s) 742
BstKTI GATC 5 cut(s) 252, 453, 746, 994, 1021
BstMAI GTCTC 4 cut(s) 506, 918, 1313, 1496
BstMBI GATC 5 cut(s) 249, 450, 743, 991, 1018
BstNI CCWGG 2 cut(s) 306, 929
BstPAI GACNNNNGTC 1 cut(s) 590
BstSCI CCNGG 2 cut(s) 304, 927
BstSFI CTRYAG 2 cut(s) 1014, 1209
BstUI CGCG 1 cut(s) 742
BstV1I GCAGC 5 cut(s) 613, 1099, 1145, 1154, 1195
BstV2I GAAGAC 1 cut(s) 1466
BstX2I RGATCY 1 cut(s) 450
BstYI RGATCY 1 cut(s) 450
Bsu36I CCTNAGG 1 cut(s) 1041
BsuRI GGCC 7 cut(s) 268, 564, 656, 1012, 1217, 1230, 1358
BtgI CCRYGG 1 cut(s) 1314
BtsCI GGATG 3 cut(s) 157, 835, 1051
BtsI GCAGTG 1 cut(s) 1370
BtsIMutI CAGTG 2 cut(s) 762, 1370
BtuMI TCGCGA 1 cut(s) 742
BveI ACCTGC 1 cut(s) 671
Cac8I GCNNGC 4 cut(s) 339, 541, 636, 1126
Cfr10I RCCGGY 1 cut(s) 638
Cfr13I GGNCC 7 cut(s) 113, 285, 301, 346, 563, 578, 1215
CseI GACGC 1 cut(s) 614
Csp6I GTAC 1 cut(s) 1032
CviAII CATG 2 cut(s) 650, 1315
CviQI GTAC 1 cut(s) 1032
DpnI GATC 5 cut(s) 251, 452, 745, 993, 1020
DpnII GATC 5 cut(s) 249, 450, 743, 991, 1018
EaeI YGGCCR 1 cut(s) 654
Eam1104I CTCTTC 1 cut(s) 1092
EarI CTCTTC 1 cut(s) 1092
Ecl136II GAGCTC 2 cut(s) 849, 1124
Eco130I CCWWGG 2 cut(s) 1314, 1359
Eco147I AGGCCT 2 cut(s) 268, 1012
Eco24I GRGCYC 2 cut(s) 851, 1126
Eco47I GGWCC 5 cut(s) 113, 285, 301, 346, 578
Eco53kI GAGCTC 2 cut(s) 849, 1124
Eco57I CTGAAG 3 cut(s) 609, 894, 1485
Eco81I CCTNAGG 1 cut(s) 1041
Eco88I CYCGRG 1 cut(s) 842
EcoICRI GAGCTC 2 cut(s) 849, 1124
EcoO109I RGGNCCY 3 cut(s) 285, 301, 1215
EcoRII CCWGG 2 cut(s) 304, 927
EcoT14I CCWWGG 2 cut(s) 1314, 1359
EcoT38I GRGCYC 2 cut(s) 851, 1126
ErhI CCWWGG 2 cut(s) 1314, 1359
FaeI CATG 2 cut(s) 653, 1318
FalI AAGNNNNNCTT 2 cut(s) 1045, 1077
FaqI GGGAC 3 cut(s) 298, 322, 1365
FatI CATG 2 cut(s) 649, 1314
FblI GTMKAC 1 cut(s) 494
Fnu4HI GCNGC 6 cut(s) 6, 627, 1113, 1134, 1143, 1209
FokI GGATG 3 cut(s) 164, 842, 1058
FriOI GRGCYC 2 cut(s) 851, 1126
Fsp4HI GCNGC 6 cut(s) 6, 627, 1113, 1134, 1143, 1209
GluI GCNGC 6 cut(s) 6, 627, 1113, 1134, 1143, 1209
GsaI CCCAGC 1 cut(s) 345
GsuI CTGGAG 1 cut(s) 274
HaeIII GGCC 7 cut(s) 268, 564, 656, 1012, 1217, 1230, 1358
HapII CCGG 2 cut(s) 171, 639
HgaI GACGC 1 cut(s) 614
Hin1I GRCGYC 1 cut(s) 606
Hin1II CATG 2 cut(s) 653, 1318
HindIII AAGCTT 1 cut(s) 1059
HinfI GANTC 2 cut(s) 191, 467
HpaII CCGG 2 cut(s) 171, 639
HphI GGTGA 2 cut(s) 36, 82
Hpy166II GTNNAC 3 cut(s) 90, 495, 1494
Hpy188I TCNGA 7 cut(s) 350, 533, 604, 918, 1180, 1335, 1413
Hpy188III TCNNGA 6 cut(s) 54, 73, 223, 291, 571, 741
Hpy8I GTNNAC 3 cut(s) 90, 495, 1494
HpyCH4III ACNGT 6 cut(s) 94, 313, 1005, 1303, 1345, 1507
HpyCH4IV ACGT 2 cut(s) 780, 795
HpyCH4V TGCA 9 cut(s) 8, 329, 686, 775, 1067, 1079, 1211, 1295, 1406
HpySE526I ACGT 2 cut(s) 780, 795
Hsp92I GRCGYC 1 cut(s) 606
Hsp92II CATG 2 cut(s) 653, 1318
Kzo9I GATC 5 cut(s) 249, 450, 743, 991, 1018
Lsp1109I GCAGC 5 cut(s) 613, 1099, 1145, 1154, 1195
LweI GCATC 4 cut(s) 403, 496, 695, 768
MaeII ACGT 2 cut(s) 780, 795
MaeIII GTNAC 4 cut(s) 553, 592, 671, 1149
MalI GATC 5 cut(s) 251, 452, 745, 993, 1020
MboI GATC 5 cut(s) 249, 450, 743, 991, 1018
MboII GAAGA 9 cut(s) 515, 708, 727, 1062, 1106, 1109, 1169, 1172, 1471
MfeI CAATTG 1 cut(s) 558
MflI RGATCY 1 cut(s) 450
MhlI GDGCHC 2 cut(s) 851, 1126
MlsI TGGCCA 1 cut(s) 656
MluCI AATT 8 cut(s) 22, 49, 196, 217, 227, 422, 558, 767
MluNI TGGCCA 1 cut(s) 656
MlyI GAGTC 1 cut(s) 185
MmeI TCCRAC 1 cut(s) 605
Mox20I TGGCCA 1 cut(s) 656
MscI TGGCCA 1 cut(s) 656
MseI TTAA 1 cut(s) 216
MslI CAYNNNNRTG 1 cut(s) 956
Msp20I TGGCCA 1 cut(s) 656
MspA1I CMGCKG 2 cut(s) 872, 1381
MspI CCGG 2 cut(s) 171, 639
MspR9I CCNGG 2 cut(s) 306, 929
MunI CAATTG 1 cut(s) 558
MvaI CCWGG 2 cut(s) 306, 929
MvnI CGCG 1 cut(s) 742
NcoI CCATGG 1 cut(s) 1314
NdeII GATC 5 cut(s) 249, 450, 743, 991, 1018
NlaIII CATG 2 cut(s) 653, 1318
NlaIV GGNNCC 7 cut(s) 77, 115, 286, 303, 347, 565, 1216
NmeAIII GCCGAG 1 cut(s) 1162
NmuCI GTSAC 3 cut(s) 553, 592, 1149
NruI TCGCGA 1 cut(s) 742
NspV TTCGAA 1 cut(s) 501
OliI CACNNNNGTG 1 cut(s) 956
PaqCI CACCTGC 1 cut(s) 671
PasI CCCWGGG 1 cut(s) 305
PceI AGGCCT 2 cut(s) 268, 1012
PfeI GAWTC 1 cut(s) 467
PflMI CCANNNNNTGG 1 cut(s) 424
PkrI GCNGC 6 cut(s) 7, 628, 1114, 1135, 1144, 1210
PleI GAGTC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 185
PpuMI RGGWCCY 2 cut(s) 285, 301
PshAI GACNNNNGTC 1 cut(s) 590
Psp124BI GAGCTC 2 cut(s) 851, 1126
Psp5II RGGWCCY 2 cut(s) 285, 301
Psp6I CCWGG 2 cut(s) 304, 927
PspFI CCCAGC 1 cut(s) 341
PspGI CCWGG 2 cut(s) 304, 927
PspN4I GGNNCC 7 cut(s) 77, 115, 286, 303, 347, 565, 1216
PspPI GGNCC 7 cut(s) 113, 285, 301, 346, 563, 578, 1215
PspPPI RGGWCCY 2 cut(s) 285, 301
PstI CTGCAG 1 cut(s) 1213
PsuI RGATCY 1 cut(s) 450
PvuII CAGCTG 1 cut(s) 872
RruI TCGCGA 1 cut(s) 742
RsaI GTAC 1 cut(s) 1033
RsaNI GTAC 1 cut(s) 1032
RseI CAYNNNNRTG 1 cut(s) 956
SacI GAGCTC 2 cut(s) 851, 1126
SaqAI TTAA 1 cut(s) 216
SatI GCNGC 6 cut(s) 6, 627, 1113, 1134, 1143, 1209
Sau3AI GATC 5 cut(s) 249, 450, 743, 991, 1018
Sau96I GGNCC 7 cut(s) 113, 285, 301, 346, 563, 578, 1215
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 2 cut(s) 306, 929
SduI GDGCHC 2 cut(s) 851, 1126
SfaNI GCATC 4 cut(s) 403, 496, 695, 768
SfcI CTRYAG 2 cut(s) 1014, 1209
SfuI TTCGAA 1 cut(s) 501
SgrAI CRCCGGYG 1 cut(s) 638
SinI GGWCC 5 cut(s) 113, 285, 301, 346, 578
SmiMI CAYNNNNRTG 1 cut(s) 956
Sse9I AATT 8 cut(s) 22, 49, 196, 217, 227, 422, 558, 767
SseBI AGGCCT 2 cut(s) 268, 1012
SsiI CCGC 1 cut(s) 1379
SstI GAGCTC 2 cut(s) 851, 1126
StuI AGGCCT 2 cut(s) 268, 1012
StyD4I CCNGG 2 cut(s) 304, 927
StyI CCWWGG 2 cut(s) 1314, 1359
TaaI ACNGT 6 cut(s) 94, 313, 1005, 1303, 1345, 1507
TaiI ACGT 2 cut(s) 783, 798
TaqI TCGA 3 cut(s) 501, 570, 689
TaqII GACCGA 1 cut(s) 635
TasI AATT 8 cut(s) 22, 49, 196, 217, 227, 422, 558, 767
TatI WGTACW 1 cut(s) 1031
TfiI GAWTC 1 cut(s) 467
Tru1I TTAA 1 cut(s) 216
Tru9I TTAA 1 cut(s) 216
TscAI CASTG 2 cut(s) 769, 1377
TseFI GTSAC 3 cut(s) 553, 592, 1149
TseI GCWGC 6 cut(s) 5, 626, 1112, 1133, 1142, 1208
Tsp45I GTSAC 3 cut(s) 553, 592, 1149
TspDTI ATGAA 5 cut(s) 15, 150, 267, 708, 799
TspGWI ACGGA 2 cut(s) 842, 897
TspRI CASTG 2 cut(s) 769, 1377
Van91I CCANNNNNTGG 1 cut(s) 424
VpaK11BI GGWCC 5 cut(s) 113, 285, 301, 346, 578
XmiI GTMKAC 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.